• Title/Summary/Keyword: Span 20

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Development of Bovine Nuclear Transfer Embryos Using Life-span Extended Donor Cells Transfected with Foreign Gene

  • Hwang, Seongsoo;Choi, Eun Joo;You, Seungkwon;Choi, Yun-Jaie;Min, Kwan-Sik;Yoon, Jong-Taek
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.11
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    • pp.1574-1579
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    • 2006
  • This study was performed to determine the developmental potentials of nuclear transfer (NT) embryos using life-span extended cells transfected with a foreign gene as donor cells. A life-span extended bovine embryonic fibroblast cell line was transfected with an expression vector in which the human type II collagen (BOMAR) and ear fibroblasts were used as a donor cell. Cytogenetic analysis was performed to analyze the chromosomal abnormality of donor cells. The fusion rate of 1.8 kV/cm for $15{\mu}sec$ given twice was significantly higher than that of other groups (p<0.05) and the embryos lysed were significantly higher after 1.8 kV/cm for $20{\mu}sec$ given once compared to other groups (p<0.01). The blastocyst development in the ear cell group was statistically significant compared to both BOMAR groups (p<0.01). Both BOMAR groups cultured more than 40 passages (>40 passages) had a lower number of chromosomes; however, fresh granulosa cell (GC) and BOMAR groups cultured less than 20 passages had normal chromosome numbers. Both >40 passages BOMAR groups had numerous obscure debris in metaphase spreads. The transfected foreign gene was expressed in all BOMAR groups, but not in the GC group. Based on these results, the lower developmental potential of NT embryos using life-span extended donor cells transfected with a foreign gene might be a cause of chromosomal abnormality in donor cells.

Nonlinear dynamic performance of long-span cable-stayed bridge under traffic and wind

  • Han, Wanshui;Ma, Lin;Cai, C.S.;Chen, Suren;Wu, Jun
    • Wind and Structures
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    • v.20 no.2
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    • pp.249-274
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    • 2015
  • Long-span cable-stayed bridges exhibit some features which are more critical than typical long span bridges such as geometric and aerodynamic nonlinearities, higher probability of the presence of multiple vehicles on the bridge, and more significant influence of wind loads acting on the ultra high pylon and super long cables. A three-dimensional nonlinear fully-coupled analytical model is developed in this study to improve the dynamic performance prediction of long cable-stayed bridges under combined traffic and wind loads. The modified spectral representation method is introduced to simulate the fluctuating wind field of all the components of the whole bridge simultaneously with high accuracy and efficiency. Then, the aerostatic and aerodynamic wind forces acting on the whole bridge including the bridge deck, pylon, cables and even piers are all derived. The cellular automation method is applied to simulate the stochastic traffic flow which can reflect the real traffic properties on the long span bridge such as lane changing, acceleration, or deceleration. The dynamic interaction between vehicles and the bridge depends on both the geometrical and mechanical relationships between the wheels of vehicles and the contact points on the bridge deck. Nonlinear properties such as geometric nonlinearity and aerodynamic nonlinearity are fully considered. The equations of motion of the coupled wind-traffic-bridge system are derived and solved with a nonlinear separate iteration method which can considerably improve the calculation efficiency. A long cable-stayed bridge, Sutong Bridge across the Yangze River in China, is selected as a numerical example to demonstrate the dynamic interaction of the coupled system. The influences of the whole bridge wind field as well as the geometric and aerodynamic nonlinearities on the responses of the wind-traffic-bridge system are discussed.

A Study on the Optimal Span Width In the Bridge Main Span of Incheon 2nd Bridge (2) Economic Analysis on Port Operational Efficiency according to Traffic Schemes in the Bridge Main Span of Incheon 2nd Bridge (인천항 제2연륙교 주경간의 적정 교각폭 결정에 관한 연구 (2) 제2연륙교 주경간의 통항방식에 따른 항만운영효율의 경제성 분석)

  • Koo Ja-Yun;Kim Seok-Jae;Jang Eun-Kyu
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2004.11a
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    • pp.337-342
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    • 2004
  • A construction project of Incheon 2nd bridge, which is connected between the Incheon Song-Do New Town and the Incheon International Airport in Young-Jong-Do, have been proposed by the private capital in 1999. But the optimal span width in the bridge main span have not been decided in spite of the three investigations into the feasibility of ship's safe transit in this planned bridge. In this paper, we study the economic analysis on port operational efficiency according to traffic schemes, me-way or two-way of vessels over 10,000G/T, in the bridge main span of this bridge. In this comparative result, total queueing time due to the one-way in the bridge main span is evaluated 20,362 hours in 2011 and 24,544 hours in 2020. Therefore the demurrage cost and the accumulation cost of freight are evaluated 19.7 billion won in 2011, and 23.3 billion won in 2020, then total accumulated costs during 33 years from 2008 until 2040 are evaluated about 768..9 billion won.

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Behavior Analysis of IPM Bridge and Rahmen Bridge (토압분리형 교량과 라멘교의 거동분석)

  • Shin, Keun-Sik;Han, Heui-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.4
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    • pp.597-605
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    • 2019
  • IPM bridge is an integral bridge that can be applied from span 30.0m up to 120.0m, the shape conditions of IPM bridge is also applicable to the rahmen bridge. In this study, to perform the structural analysis of Rahmen bridge and IPM Bridge, the researchers compared the distribution types such as load, moment, and displacement of those bridges. Structural analysis was carried out on four span models ranging from single span bridges to four spans of 120.0 m, based on span length of 30.0 m. Structural analysis was carried out on those bridge with span 30.0m up to 120.0m. The conclusions drawn from this study are as follows. 1) The bending moments were calculated to be large for the Rahmen bridge, and the horizontal displacements were estimated to be large for the IPM bridge. 2) Since the bending moments are derived by the span length rather than the extension of the bridge, the permissible bending moment for the span length should be considered in the design. 3) The pile bent of the IPM bridge did not exceed the plastic moment of the steel pipe pile at 120.0m span, but because the horizontal displacement in the shrinkage direction is close to 25mm, the design considerations are needed. 4) In the actual design, it is important to ensure stability against member forces, so review of the negative moment is most important.

Competitive Displacement of Methylcellulose from Oil-Water Interface by Various Emulsifiers (저분자량 유화제 첨가에 의한 계면 흡착 메칠셀룰로오스의 경쟁이탈 특성 연구)

  • Hong, Soon-Taek
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.10
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    • pp.1271-1277
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    • 2008
  • Competitive displacement of methylcellulose (MC) absorbed at the oil-water interface was investigated by interfacial composition, surface shear viscosity, or surface tension measurements. It was found that all emulsifiers could competitively displace the interfacial MC from the oil-water interface but their behaviors were different from each other. With Tween 20 added to MC emulsion (1 wt% MC, 10 wt% n-tetradecane, 20 mM bis-tris, pH 7), MC load was steadily decreased with increasing concentrations of the emulsifier, as confirmed by surface shear viscosity measurements; moreover, there was complete MC displacement from the emulsion droplet surface at high concentration (0.1 wt%). The oil-soluble Span 80 was found to show a synergism with MC at the interface, which resulted in higher MC load at relatively low emulsifier concentrations ($\leq$0.05 wt%). At a higher emulsifier concentration (0.1 wt%) limited MC displacement was observed. These results were well supported by surface shear viscosity measurements. With water-soluble SDS, MC load was decreased with increasing concentrations of the emulsifier. Unlike Tween 20, however, it was found that at high concentrations (> 0.1 wt%), there was still some MC remaining at the droplet surface. Surface tension measurements are suggestive of an interfacial complex between MC and SDS.

Detergency and Water Wetting/Retention Properties of Soiled Cotton Cloths in Nonionic Surfactant Solutions (비이온계 계면활성제 수용액에서 면 오염포의 습윤특성과 세척성)

  • Kim, Chun-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.3 s.162
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    • pp.433-439
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    • 2007
  • The effects of nonionic surfactants on detergency and water wetting/retention properties of soiled cotton cloths were reported. Two different soiled cloths were used. soiled cotton cloth 1 was made in the lab. with carbon black, tripalmitin, n-dodecane & palmitic acid on Korea Apparel Testing & Research Institute(KATRI) cotton testcloth and soiled cotton cloth 2(EMPA 101) was purchased from Testfabrics, Inc., USA. The following nonionic surfactants; l.e., Span 20, Tween 20, 40, 60, 80, 21, 61, 81, 65, & 85, were used in the study. The water retention ratio(W/H) values of soiled cotton cloths were decreased, whereas the water contact angle values of soiled cloth were not changed markedly compared with those of unsoiled testcloths. The wetting and water retention of soiled cotton cloth 1 was improved with addition of nonoinic surfactants. The surfactants which have more hydrophilic characterictics or unsaturated hydrophobe tails were effective in improving wetting and water retention properties of soiled cotton cloth 1. The water contact angle values of soiled cotton cloths were extremely low with Span 20, presumably due to the high adsorption density or the surfactant. The detergency of soiled cloths were low in Span 20 and high in Tween 20, 40, 60 & 80 0.1g/dl surfactant solutions. Nonionic surfactants having higher ethylene oxide contents resulted in better detergency. In the range studied, the wetting and water retention of soiled cotton cloths did not show any particular relation to the detergency, whereas the surfactant characteristics, especially HLB values, influenced the detergency of soiled cotton cloths.

Fiber optic shape sensor system for a morphing wing trailing edge

  • Ciminello, Monica;Ameduri, Salvatore;Concilio, Antonio;Dimino, Ignazio;Bettini, Paolo
    • Smart Structures and Systems
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    • v.20 no.4
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    • pp.441-450
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    • 2017
  • The objective of this work is to present a conceptual design and the modelling of a distributed sensor system based on fiber optic devices (Fiber Bragg Grating, FBG), aimed at measuring span-wise and chord-wise variations of an adaptive (morphing) trailing edge. The network is made of two different integrated solutions for revealing deformations of the reference morphing structure. Strains are confined to typical values along the span (length) but they are expected to overcome standard ranges along the chord (width), up to almost 10%. In this case, suitable architectures may introduce proper modulations to keep the measured deformation low while preserving the information content. In the current paper, the designed monitoring system combines the use of a span-wise fiber reinforced patch with a chord-wise sliding beam. The two elements make up a closed grid, allowing the reconstruction of the complete deformed shape under the acceptable assumption that the transformation refers to regular geometry variations. Herein, the design logic and some integration issues are reported. Preliminary experimental test results are finally presented.

Wind Pressure Spectra for Circular Closed and Open Dome Roofs (원형 밀폐 및 개방형 돔 지붕의 풍압 스펙트럼)

  • Cheon, Dong-jin;Kim, Yong-Chul;Lee, Jong-Ho;Yoon, Sung-Won
    • Journal of Korean Association for Spatial Structures
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    • v.20 no.2
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    • pp.69-76
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    • 2020
  • Wind tunnel tests were conducted to analyze the wind fluctuating pressures on a circular closed and open dome roof with a low span rise. Two dome models with various geometric parameters (height/span ratios and open ratios) were used for fixed span rise ratio dome and wind pressure spectrum were analyzed. The applicability was examined in comparison with the spectral model proposed in the previous studies. The analysis results show that the wind pressure spectrum of open dome roof tends to increase power in the high frequency range and the second peak is found in the area different from the closed dome roof. In addition, according to the comparison analysis with the previous proposed spectral model, it was found that it is not applicable to the closed and open dome roofs with low rise ratio due to the different peak frequencies.