• Clinical and Experimental Reproductive Medicine
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• 제47권3호
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• pp.186-193
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• 2020

Objective: The effectiveness of Staphylococcus protein A (SPA) in improving the penetration ability of sperm and reducing antisperm antibody (ASA) titers in immunologically infertile males was evaluated. Methods: Seminal fluid samples were obtained from 15 infertile men, and ASA titers were assessed with the latex agglutination test. Identification of immunoglobulin (Ig) classes and characterization of the antigens involved in the immune response were performed using indirect immunofluorescence. Local ASAs typically present as a mixture of IgG and IgA classes. The capillary tube penetration method was used to assess the capability of spermatozoa to penetrate the cervical mucus (CM). Results: ASAs associated with the neck region of sperm showed a significantly lower migration distance in the CM of infertile females than ASAs associated with the head or tail segments. ASA-positive seminal fluid exhibited significant increases in the mean migration distance (2.6 ± 1.4 cm vs. 1.54 ± 1.1 cm, respectively; p< 0.001) and sperm concentration (174 ± 121.0 × 10³/mL vs. 101 ± 93.7 × 10³/mL, respectively; p= 0.033) after treatment with SPA compared to pre-treated samples. A significant reduction (p< 0.01) in the recorded ASA titer was detected. Conclusion: These results indicate that SPA can be used as a sorting regimen for insemination programs. However, further studies are warranted to assess its influence on pregnancy rate.

• 한국콘텐츠학회논문지
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• 제7권12호
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• pp.191-198
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• 2007

본 연구의 목적은 수안보 온천제의 참관객들이 축제의 운영성과, 행사내용, 축제 이미지, 편의시설에 대한 평가와 이에 대한 호응도와 추천의도를 알아보기 위함이다. 연구의 결과는 다음과 같다. 첫째, 축제평가에서 60대의 노인계층이 축제를 긍정적으로 평가하였다. 이는 온천축제의 성격상 노인계층에게 많이 어필되기 때문이다. 따라서 노인계층에게 소구될 수 있는 전통차 만들기, 허브 마사지의 건강 체험중심의 Eco-therapy 프로그램을 제공함으로써 더 많이 유인할 수 있을 것이다. 둘째, 축제 이미지와 편의시설은 높은 호응도뿐만 아니라, 주변지인들에게 강력한 추천의도를 갖고 있는 것으로 나타났다. 활쏘기, 말타기, 가마타기, 사극복장입기의 전통체험중심의 내용을 통하여 이미지 제고와 편안히 쉴 수 있는 편의 및 휴식공간을 제공해야 할 것이다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 춘계학술대회
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• pp.219-222
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• 2013

현재 부채널 공격은 P. Kocher에 의해 제안된 공격 방법으로 암호 알고리즘 자체의 안전성이 높다고 하더라도 암호 알고리즘이 구현된 방법이나 구현된 환경에 따라 적용이 가능한 공격법이다. 이러한 부채널 공격은 정보보호 장치의 전력(전압), 실행시간, 오류 출력, 소리 등의 변화를 측정해 각종 정보의 이진코드를 읽어내어 통계적인 방법으로 중요한 정보를 분석함은 물론 위 변조까지 가능하다. 따라서 본 논문에서는 부채널 공격 기법 중에 하나인 SPA 공격 기법과 DPA공격 기법을 살펴보고 현재까지 나온 최신 대응 기술들을 분석하고자 한다.

• 대한안전경영과학회지
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• 제7권4호
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• pp.141-163
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• 2005

The international SPICE (Software Process Improvement and Capability determination) Project ISO/IEC 15504(SPICE : Software Process Improvement and Capability determination) is an emerging International Standard on SPA(Software Process Assessment). A prime motivation for developing this standard has been the perceived need for an internationally recognized software process assessment framework that pulls together the existing public and proprietary models and methods. A SPICE assessment can be considered as one of representative SPA model since assessors assign ratings to indicators and metrics to measure the capability of software process. But this models doesn't provide a systematic measurement procedures and dynamic method for SPI(Software Process Improvement). Through the evaluation of SPICE is capable of providing a substantiated basis for using the notion of capability, as well as providing information for nacessary improvements to the standard using 6sigma process. As a result, this paper propose a measurement procedure and guidelines for application of 6sigma process to guarantee the reliability in SPI and suggest the structure to support SPI on overall organization.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2011년도 30주년 정기총회 및 국제심포지엄
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• pp.188-195
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• 2011

Red lateritic soils are typically low in total organic carbon (TOC) and available phosphorus (AP) content and continuous fertilization is required to obtain desired crop yield. In this experiment, cattle manure in three forms (air-dried, composted and vermicomposted) were applied to red lateritic soil to study their effect on TOC and AP content of soil and probable mechanism of P-solubilization as affected by these treatments were also studied. Vermicompost was the most effective to solubilize insoluble P in red lateritic soil (Alfisols) as compared to other organic amendments (air-dried cattle manure and compost). The highest SPA in vermicompost-treated soil attributed to the comparatively higher concentration of all the three SPA isozymes in these soils. The maximum P-solubilization in these soils might be attributed to the highest SPA and presence of several organic acids like citric, lactic and oxalic acids in vermicompost-treated soils. Since, vermicompost application also increased TOC, mineralizable N and exchangeable K content of soil, vermicompost could be considered as the most rational organic amendment to improve chemical properties of red lateritic soils.

• International Journal of Oral Biology
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• 제44권2호
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• pp.31-36
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• 2019

Streptococcus mutans is one of the important bacteria that forms dental biofilm and cause dental caries. Virulence genes in S. mutans can be classified into the genes involved in bacterial adhesion, extracellular polysaccharide formation, biofilm formation, sugar uptake and metabolism, acid tolerance, and regulation. The genes involved in bacterial adhesion are gbps (gbpA, gbpB, and gbpC) and spaP. The gbp genes encode glucan-binding protein (GBP) A, GBP B, and GBP C. The spaP gene encodes cell surface antigen, SpaP. The genes involved in extracellular polysaccharide formation are gtfs (gtfB, gtfC, and gtfD) and ftf, which encode glycosyltransferase (GTF) B, GTF C, and GTF D and fructosyltransferase, respectively. The genes involved in biofilm formation are smu630, relA, and comDE. The smu630 gene is important for biofilm formation. The relA and comDE genes contribute to quorumsensing and biofilm formation. The genes involved in sugar uptake and metabolism are eno, ldh, and relA. The eno gene encodes bacterial enolase, which catalyzes the formation of phosphoenolpyruvate. The ldh gene encodes lactic acid dehydrogenase. The relA gene contributes to the regulation of the glucose phosphotransferase system. The genes related to acid tolerance are atpD, aguD, brpA, and relA. The atpD gene encodes $F_1F_0$-ATPase, a proton pump that discharges $H^+$ from within the bacterium to the outside. The aguD gene encodes agmatine deiminase system and produces alkali to overcome acid stress. The genes involved in regulation are vicR, brpA, and relA.

• 정보보호학회논문지
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• 제13권3호
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• pp.35-43
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• 2003

타원곡선 암호시스템에서 스칼라 곱셈의 랜덤화는 부-채널공격 대응방법의 기본적인 개념 중의 하나이다. 본 논문에서는 랜덤 m-ary 리코딩 알고리듬에 기반한 랜덤 m-ary 방법으로 단순/차분 전력분석 공격의 대응 방법을 제안한다. 제안 방법은 표준의 m-ary 방법보다 부가적인 연산량이 요구되지만 비밀키와 독립적인 소모전력을 생성한다. 따라서 랜덤한 윈도우 사이즈를 이용한 연산 과정이 SPA/DPA 공격에 대응할 수 있으므로 제안하는 대응방법은 스마트카드의 부-채널공격에 향상된 안전성을 제공한다.

• 정보보호학회논문지
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• 제14권4호
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• pp.135-140
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• 2004

ID 기반 암호 시스템과 전력 분석 공격(Power Analysis Attack)은 모두 각각의 분야에서 활발한 연구가 진행되는 주제이다. 특히 DPA(Differential Power Analysis) 공격(2)은 스마트카드와 같은 저전력 장치에 대한 가장 강력한 공격방식으로 취급되어 왔다. 그러나 ID 기반 암호 시스템과 전력 분석 공격은 각기 독립적으로 연구되고 있다. 본 논문에서는 전력 분석 공격이 ID 기반 암호 시스템의 안전도에 미치는 영향에 대해 분석한다. 그 결과로, pairing을 사용하는 ID 기반 암호 시스템의 경우 DPA에 대한 대응책 없이 SPA에 대한 대응책만으로도 충분히 안전하다는 것을 보인다.

• 자동차안전학회지
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• 제14권1호
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• pp.45-54
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• 2022

Recently, the introduction of Cooperative Intelligent Transport Systems (C-ITS) has been attempted to solve the limitation of only the sensor of the vehicle itself. For example, vehicles traveling at intersections can drive more safely through C-ITS. By using V2X communication of WAVE and LTE, the driver can receive the status and time of traffic lights. However, LTE has a larger transmission delay time than WAVE, so timimg data may not match in real time. In this paper, using the SPaT message, it was confirmed that LTE has a larger C-ITS service transmission delay time than WAVE. Finally, it was confirmed that the timing data of SPaT provided by LTE corrected by the algorithm is similar to SPaT provided by WAVE. It was confirmed that safer intersection driving is possible based on real-time.

• Clinical and Experimental Reproductive Medicine
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• 제21권1호
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• pp.1-11
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• 1994

The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.