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Distribution of Arbuscular Mycorrhizal Fungi in the Soil grown Tomato Plants under Greenhouse (시설재배 토마토 토양에서 Arbuscular 균근균의 분포)

  • Cho Ja-Yong;Kim Jin-Seop;Yang Seung-Yul
    • Korean Journal of Organic Agriculture
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    • v.14 no.2
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    • pp.219-228
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    • 2006
  • This study was conducted to examine the distribution of arbuscular mycorrhizal fungi (AMF) in the soil grown tomato plants in Damyang districts. We collected twenty one soil samples from the rhizosphere of tomato plants which were grown under structure. Number of spores/g in the soil sized over $500{\mu}m,\;355{\sim}500{\mu}m,\;251{\sim}354{\mu}m,\;107{\sim}250{\mu}m\;and\;45{\sim}106{\mu}m$ were 0.01, 0.02, 0.09, 0.9, and 2.0. Total number of spores/g in the fresh soil were 3.02. Mycorrhizal root infection by vesicles, hyphae and arbuscules were 18.0%, 6.0% and 2.0%. To identify the genus of arbuscular mycorrhizal fungi, isolated mycorrhizal spores from the soil grown tomato plants were inoculated into the host plant of sudangrass and mass propagated for 4 months. As a result of identification, mycorrhizal spores were identified as Glomus sp., Gigaspora sp. and Acaulospora sp.

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Harmful Microorganisms Occurred on the Bed-logs of Several Quercus spp. for Shiitake Cultivation (표고재배시 여러 종류의 참나무골목에 발생되는 해균상)

  • Park, Won-Chull;Ka, Kang-Hyeon;Yoon, Kab-Hee;Ryu, Sung-Ryul;Lee, Bong-Hun
    • The Korean Journal of Mycology
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    • v.35 no.1
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    • pp.33-36
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    • 2007
  • An attempt was made to investigate the status of harmful microorganisms occurring on different kinds of oak bed-logs during shiitake cultivation. As a result, totally 14 species of harmful microorganisms, including Trametes versicolor, were confirmed. Twelve kinds of harmful microorganisms were observed on Quercus acutissima, 9 kinds on Q. mongolica and 10 kinds on Q. aliena. Diatrype stigma, Hypoxylon truncatum, Hypoxylon sp. and Trichoderma sp. occupied 75.1% of the total harmful fungi occurred on Q. acutissima. H. truncatum and Trichoderma sp. occupied 71.2% of the total harmful fungi occurred on Q. mongolica. On Q. aliena, the occurrence of H. truncatum, Trichoderma sp. and Hypoxylon howeianum was 80.3%. D. stigma and Hypoxylon sp. were observed exclusively on Q. acutissima bed-logs, and the outbreak ratios were 51.6% and 13.1%, respectively. H. truncatum was observed on 46.6% of Q. aliena bed-logs and Trichoderma sp. was observed on 30.3% of Q. mongolica bed-logs.

Adsorption Properties and Production of Biosorbent from Bacillus sp. SK31 (Bacillus sp. SK3l의 생물흡착제 흡착특성 및 생산)

  • 서현호;김형갑
    • Journal of Korea Soil Environment Society
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    • v.4 no.2
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    • pp.33-43
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    • 1999
  • A strain (designated SK3l) which produces an excellent adsorption substance was isolated from soil samples and identified as Bacillus specied. The major adsorption substance (biosorbent SK3l) produced by Bacillus sp. SK31 was purified by ethanol precipitation and cetylpyridinium (CPC) precipitation. The adsorption charactics of zinc and lead ions on bioadsorbent SK3l were investigated. The equilibrium isotherms showed that bioadsorbent SK3l took up zinc and lead from aqueous solutions to the extent of about 52 mg/g and 112 mg/g. respectively. The culture conditions at the flask level of Bacillus sp. SK3l were investigated for the production of polysaccharide bioadsorbent, SK3l. The optimum pH and temperature for sorbent production were 7.5 and $30^{\circ}C$, respectively. The important carbon and nitrogen sources for sorbent formation were glucose and ammonium nitrate, respectively. In the optimized medium, sorbent production was improved three folds in comparison with the basal medium. In the jar fermenter, the highest sorbent production was obtained at 60 h cu1tivation time and the amount of biosorbent SK3l at that time was 9.2 g/$m\ell$.

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Studied on the Antibacterial, Antifungal Components in Some Korean Marine Sponges (한국산 해면류중의 항균, 항곰팡이 물질에 관한 연구)

  • LEE Jong-Soo;KIM In-Soo;MOON Soo-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.24 no.3
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    • pp.193-202
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    • 1991
  • Antimicrobial substances were screened by paper disk plate method in marine sponges, Halichondria okadai, Halichendria sp., H iaponica and Haliclona Pemollis, collected from the south coast of Korea. Antibacterial components were detected in two species, H okadai and Halichondria sp.. Three components such as benzoic acid, okadaic acid(OA) and dinophysistoxin-1(DTX1) were identified from these sponges as the antimicrobial compounds by MS and NMR spectral data. OA$(550{\~}600{\mu}g/kg)$ and $(400{\~}490{\mu}g/kg)$ were determined from the wet H okadai and Halichondria sp., respectively, by using fluorometric HPLC analysis with 9-anthryldiazomethane(ADAM) as fluorescent labelling reagent.

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A Study on the Combustion Characteristics of Paraffin wax/LDPE Blended fuel (Paraffin wax/LDPE 혼합 연료의 연소 특성에 관한 연구)

  • Kim, Soo-Jong;Cho, Jung-Tae;Lee, Jung-Pyo;Moon, Hee-Jang;Sung, Hong-Gye;Kim, Jin-Kon
    • Journal of the Korean Society of Propulsion Engineers
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    • v.14 no.2
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    • pp.29-38
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    • 2010
  • The experimental study on paraffin wax/LDPE blended fuel for hybrid rocket was performed. Various combustion characteristics of blended fuel were compared with pure paraffin, HTPB, HDPE and SP-1a fuel in order to evaluate the performance of blended fuel. The regression rate of lab-scale and large-scale motor using pure paraffin fuel was increased by 10.2 and 9.8 factor when respectively compared to that of HDPE. The regression rate factor of blended fuel was 3.4 in which the regression rate of blended fuel was higher than that of HTPB and HDPE, but lower than that of pure paraffin, SP-1a fuel. The values of characteristic velocity and specific impulse of blended fuel was higher than those of pure paraffin, HTPB and HDPE, and almost the same as SP-1a fuel. As these results, it was confirmed that blended fuel can be an effective solid fuel for hybrid rocket.

Disinfection of Culture Water Supply by Ozonization I. Susceptibility of Some Fish-Pathogenic Bacteria Isolated From Cultured Marine Fish (오존처리법에 의한 양어용수 살균에 대하여 I. 해산어류 병원세균의 오존 감수성)

  • Oh, Myung-Joo;Kim, Heung-Yoon;Cho, Hyun-Soh
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.42-48
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    • 1999
  • The disinfectant effects of total residual oxidants (TROs) produced by ozonization of natural sea water were investigated against fish pathogenic bacteria isolated from flounder and red seabream. The concentration of 0.1 mg TROs/liter was stable for 20 min in filtered natural seawater, and those of 0.3 and 0.5 mg TROs/liter were also stable for more 1 hr. Disinfectant effects of TRO against Edwardsiella tarda, Vibrio sp., Streptococcus sp. and Staphylococcus sp. were observed with a concentration of 0.1 mg/liter for 180 sec, and the treatment killed more than 99.9% of bacterial cells. With TROs of 0.3 to 0.5 mg/ liter, the viable cells of the bacteria were reduced by more than 99.99% in 60 sec.

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Dispersion Stability Determination of Saengshik Beverage by Optical Methods (광학적 방법에 의한 생식음료의 분산 안정성 측정)

  • Lee, Ju-Yeon;Mok, Chulkyoon
    • Food Engineering Progress
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    • v.14 no.1
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    • pp.41-48
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    • 2010
  • An optical method was introduced to investigate the dispersion stability of Saengshik beverages (SB) containing 3.7-11.7% Saengshik powder (SP). Time course changes in backscattering light flux (BSLF) from SB were monitored by a Turbiscan. BSLF in the bottom and top layers of SB increased by forming sediment and foam, respectively, while that in the middle layer decreased by clarifying. With SP levels, sedimentation in SB was retarded, but the height of total sedimentation layer was increased. A logarithmic model was developed to fit to the changes in BSLF with time, showing determination coefficients of 0.979-0.988. The levels of SP in SB influenced the migration speed of the clarification front as well as the numbers of separated layers; 2 layers in SB containing 3.7% SP, 3 in 5.7-9.7%, and 4 in 11.7%. Formation of clear layers started after 17-29 min and continued for 22-53 min. The clarification was retarded most in SB containing 7.7% SP, indicating the most stable SP level for the SB preparation.

Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea (닭의난초(Epipactis thunbergii)에 공생하는 난 균근균의 분리 및 동정)

  • Han, Han-Kyeol;Chung, Jae-Min;Cho, Yong-Chan;Kim, Dae-Shin;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.41 no.1
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    • pp.9-13
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    • 2013
  • In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.

Remediation of PAH-Polluted Soil by Pseudomonas sp. Adhered on PU Foam (PU매체에 부착한 유류분해 bacteria를 이용한 오염토양 처리)

  • Cho Dae-Chul;Huh Nam-Soo;Kwon Sung-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.3
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    • pp.458-464
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    • 2006
  • Bioremediation has been recognized as one of the best tools for hydrocarbon contaminated soil and nearby groundwater which had been heavily polluted in industrial areas. Degradation of PAHs in PAH-polluted loam soil were investigated under polyurethane foam environment with adsorbed bacteria Pseudomonas sp. (KCCM 40055) in order to acquire vital data for the environmentally-friendly process and material. macroporous commercial polyurethane foam that is widely used for microbial attachment in such as sewage treatment was selected for experiments. We also examined the microbial adherence upon the media. SR9-35C/G among the PU samples showed the highest degree of attachment and bioconversion. The conversion efficiency increased with moisture content of soil.

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Studies on the pediculosis of Korean native goats (Capra hircus coreanae) in Chonnam area (전남지방에서 사육되는 흑염소의 이감염증에 관한 연구)

  • Kwag, Hyoung-Su;Lee, Jeong-Chi;Shin, Sung-Shik;Kim, Sang-Ki;Lee, Chai-Yong;Lee, Chung-Gil
    • Korean Journal of Veterinary Research
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    • v.42 no.4
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    • pp.531-536
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    • 2002
  • A total of 576 fatteners selected randomly from 6 Korean native goat farms in Chonnam area were examined for louse infestation from March 1997 to February 1998. The lice collected from the goats were identified. Four goat farms were selected and 20 goats were randomly chosen from each farm; blood samples were taken from the goats seasonally and examined hematologically and chemically. It was found that 495 out of 576 goats (85.9%) were infested with lice. The infestation rate in one of the 6 farms reached 92.9%. Two species of lice were identified; Linognathus sp. (sucking lice) and Bovicola sp. (biting lice). Goats infested with lice showed higher values in white blood cell, neutrophil and eosinophil counts than the goats not infested counterparts; same trend was seen with total protein, albumin and globulin concentrations. The results of this study indicate that louse infestation in the goat should be investigated extensively in view of the goat farming.