• The Plant Pathology Journal
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• v.26 no.2
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• pp.130-138
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• 2010

Orchid fleck virus (OFV) is an unassigned plant virus in the family Rhabdoviridae. OFV was isolated from Cymbidium sp. showing oval necrotic lesions on their leaves in Korea, and designated as OFV-NHHS1. The complete nucleotide sequence of the RNA1 (6,413 nt) (GenBank accession no. AB516442) and RNA2 (6,001 nt) (GenBank accession no. AB516441) was determined in this study. RNA1 and RNA2 contained five and one ORF respectively. RNA1 encodes nucleocapsid (N) of 49 kDa, ORF2 of 26 kDa, ORF3 of 38 kDa, ORF4 of 20 kDa and glycoprotein (G) of 61 kDa proteins, whereas RNA2 encodes a single polymerase of 212 kDa. OFV-NHHS1 shared extremely high similarity of 98.6-100% and 98.9-99.6% in nucleotidle and amino acid sequences with a Japanese isolate, OFV-so, respectively. However, the N, G and L of OFV-NHHS1 revealed 6.9-19.3%, 7.3-12.0%, and 13.4-26.6% identities to those of 29 Rhabdoviruses, respectively. To survey the infection rate of OFV in commercial orchids in Korea, 51 Cymbidium sp., 10 Phalaenopsis sp., 22 Oncidium sp. and 21 Dendrobium sp. plants that showed typical viral symptoms were collected. RT-PCR with specific primers for detection of Cymbidium mosaic virus (CymMV), ORSV and OFV showed high infection rate by ORSV alone and double infection by ORSV and CymMV. One of the orchids tested was infected with OFV. This is the first report of the complete nucleotide sequences of OFV isolated in Korea.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.121-126
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• 1988

Efficacy of praBiquantel (CesocideB injection) by intramuscular (1.M.) route against cestode infections was evaluated. Total 93 domestic or laboratory animals such as dogs, cats, rats, mice, goats, deers and chickens were used. Animals were infected with Dipylidium caninum, Spirometra sp. , Taenia pisiformis, Taenia taeniaeformis, Hymenolepis nana, Moniegia expanse, Moniexia sp. or Raillietina sp. A single dose of prasiquantel, 6 mg/kg of body weight, was highly effective (97.9%) against cestodes of various kinds disregarding the host species or their intensity of infection. At higher dose above 6 mg/kg, the cure rate was 100%. All the cestodes treated were expelled from the host within 48 hours. The discharged proglottides were damaged severely except Hymenolepis nana and Moniegia expanse. Intramuscular injection of this drug evoked a brief pain response in a dog, but no other side reactions were observed.

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.400-407
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• 2009

The purpose of this study is to investigate hypotensive effect of BDR-29, new herbal preparation of Cassiae Semen, Prunellae Spica, Uncariae Ramulus et Uncus, and Tribuli Semen, in stroke-prone spontaneously hypertensive rats (SHR-SP). SHR-SP were treated with BDR-29 at a dose of 100, 200 mg/kg/day orally for 13 weeks. In the BDR-29 treat group, mean blood pressure and systolic blood pressure were significantly reduced (p<0.05). In phenylephrine-precontracted arota and carotid artery, BDR-29 induced endothelium-dependent vascular relaxation. Hematological findings and biochemical examination revealed no evidence of specific toxicity related to BDR-29. In addition, BDR-29 was markedly attenuated intima-media thickness of thoracic aorta with progression of atherosclerosis. SHR-SP were treated with BDR-29 were significantly increased eNOS expression in arota. These results indicated that BDR-29 improves blood pressure as well as initial atherosclerotic lesion.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.279-284
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• 2010

Four leptocephali (TL 109.8~129.7 mm) of the family Ophichthidae, collected from Sangju in the South Sea of Korea, were identified using morphological and molecular methods. Our four leptocephali were similar to Ophichthus sp. based on morphological characters: the melanophores present in series of eight gut swellings; total myomeres 144~151; body depth in total length less than 10%; eight or nine black horizontal bands present from anus to caudal fin base. On the other hand, our four leptocephali were identified to Pisodonophis sp. based on 826 base pairs of 12S rRNA gene nucleotide sequences. Our results showed that morphology-based identification did not agree with molecular identification, indicating difficulty in differentiating morphologically between Ophichthus and Pisodonophis leptocephali. We, herein, firstly describe morphological characteristics of Pisodonophis sp. leptocephali from Korea.

• Journal of Environmental Science International
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• v.25 no.3
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• pp.425-438
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• 2016

This study carried out zeta potential measurements of the Microcystis sp. under various solutions condition and investigated the characteristics of Microcystis sp. through the size control of microbubbles to eliminate algae that causes problems in aquatic ecosystems and human activities. DAF process was adopted and several coagulants were used to remove the Microcystis sp. CCD Camera was used to measure and analyze the size of microbubble, and fluorescent microscope was used to observe the particle, algae species and community. Zeta potential behavior of the algae was analyzed by using ELS-Z. Lab-scale and pilot-scale experiments were conducted to test flotation process. Polyaluminium chloride(PAC) coagulant was used, and the removal efficiency of the algae was assessed through Chlorophyll-a analysis. In the Lab-scale experiment, 2.2 ppm, 11 ppm, 22 ppm, and 44 ppm of polyaluminium chloride was injected to coagulate the algae. The coagulated algae was floated by the microbubble. The microbubbles in the experiments were generated at a air pressure of 450 ~ 550 kPa. The microbubble size was controlled in $36{\mu}m$, $100{\mu}m$, and $200{\mu}m$, respectively by using different diffuser. The results of lab-scale experiments on flotation plant indicated that the average removal rate was about 90% or above for 11 ppm, 22 ppm, and 44 ppm of polyaluminium chloride. On the other hand, in the pilot-scale experiment, the removal efficiency was in the range of 85% to 95% in all dose ranges of polyalumium chloride and aluminium sulfate coagulants.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.482-488
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• 2010

Three specimens of Cynoglossidae larvae were collected from the southern Korean Sea in May and August of 2009, and were identified using morphological and molecular analysis. Specimens were divided into two groups based on the number of elongated dorsal fin rays on the top of the head: Cynoglossidae sp. A was defined as having two elongated dorsal fin rays, while Cynoglossidae sp. B possessed a single elongated dorsal fin ray. One specimen of Cynoglossidae sp. A, a post-larva with a notochord length (NL) of 5.8 mm was thought to be a Cynoglossus joyneri larva based on the presence of 115 dorsal pterogiophores, 85 anal pterogiophores, and 50 myomeres. Two specimens of Cynoglossidae sp. B, a 4.1 mm NL larva and a 11.3 mm NL juvenile, were thought to be Cynoglossus abbreviatus based on the presence of yolk in the former and 133 dorsal fin rays, 105 anal fin rays, and 63 myomeres in the latter. To test this morphology-based identification, molecular analysis was conducted using 419-422 bp of mitochondrial DNA 16S rRNA. Cynoglossidae sp. A was clearly matched to a Cynoglossus joyneri adult (d=0.000) and Cynoglossidae sp. B clustered closely with Cynoglossus abbreviatus adults (d=0.002). A neighbor-joining tree supported this robust relationship (bootstrap value=100%). Therefore, these molecular data validate the morphological identification of the two Cynoglossidae larval species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.139-143
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• 1999

The antimicrobial action of bacteriocin produced by lactobacillus sp. GM7311 against three Gram negative bacteria, Proteus mirabilis, Vibrio parahaemolyticus, and Escherichia coli, was investigated, When the bacteriocin was added to the culture at different stages, viable cells of all of the indicator strains tested were decreased, even though the most inhibited indicator cell growth stages were different. Transmission electron microscopic observation of indicator strains treated with bacteriocin revealed cell Iysis, indicating the cell membrane appears to be the primary site of action. The amino acids concentration of indicator strains treated with bacteriocin were diminished and fatty acids compositions were changed as compared with controls.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.2
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• pp.77-85
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• 2006

Entomopathogenic nematodes (EPNs) have been used as biological control agents for control of various agro-forest insect pests, and are especially effective against soil-dwelling insect pests. Effect of soil moisture on pathogenicity of commercial EPNs for white grub control was evaluated in laboratory, pots, and golf courses. Pathogenicity of EPNs in sand column was variable depending on depth, soil moisture, and EPN species or strain. All tested EPNs (Heterorhabditis sp. GSNUH1, Heterorhabditis sp. GSNUH2, Steinernema carpocapsae GSN1, and S. longicaudum Nonsan strain) showed similar pathogenicity against the bait insect, great wax moth (Galleria mellonella) larva at 2 cm deep at a given soil moisture. However, pathogenicity of the Heterorhabditis sp. GSNUH1 strain was decreased with increasing soil moisture. Pathogenicity of S. carpocapsae GSN1 strain was the lowest in 3% soil moisture (v/w) at 7 cm depth. However, there was no difference in pathogenicity between Heterorhabditis sp. GSNUH2 and S. longicaudum Nonsan strain. Although pathogenicity of Heterorhabditis sp. KCTC 0991BP strain showed no difference against the 2nd instar of Exomala orientalis, that of the S. carpocapsae GSN1 strain was decreased in the laboratory depending on soil moisture. Highly pathogenic strain EPN, Heterorhabditis sp. KCTC 0991BP strain, showed higher pathogenicity at 100 mm irrigation than non-irrigation or 10 mm irrigation. However, poor pathogenic strain EPN, S. carpocapsae GSN1 strain, was not different in pathogenicity from the 2nd instar of Exomala orientalis in creeping bentgrass (Agrostis palustris) depending on irrigation amount in the pot. Pathogenicity of EPNs in field experiment at the tee of Ulsan golf club showed a similar trend to that in the pot experiment.

• Molecules and Cells
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• v.23 no.3
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• pp.370-378
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• 2007

A superoxide dismutase was purified 62-fold in seven steps to homogeneity from Methylobacillus sp. strain SK1, an obligate methanol-oxidizing bacterium, with a yield of 9.6%. The final specific activity was 4,831 units per milligram protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. The molecular weight of the native enzyme was estimated to be 44,000. Sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 23,100. The isoelectric point of the purified enzyme was found to be 4.4. Maximum activity of the enzyme was measured at pH 8. The enzyme was stable at pH range from 6 to 8 and at high temperature. The enzyme showed an absorption peak at 280 nm with a shoulder at 292 nm. Hydrogen peroxide and sodium azide, but not sodium cyanide, was found to inhibit the purified enzyme. The enzyme activity in cell-free extracts prepared from cells grown in manganese-rich medium, however, was not inhibited by hydrogen peroxide but inhibited by sodium azide. The activity in cell extracts from cells grown in iron-rich medium was found to be highly sensitive to hydrogen peroxide and sodium azide. One mol of native enzyme was found to contain 1.1 g-atom of iron and 0.7 g-atom of manganese. The N-terminal amino acid sequence of the purified enzyme was Ala-Tyr-Thr-Leu-Pro-Pro-Leu-Asn-Tyr-Ala-Tyr. The superoxide dismutase of Methylobacillus sp. strain SK1 was found to have antigenic sites identical to those of Methylobacillus glycogenes enzyme. The enzyme, however, shared no antigenic sites with Mycobacterium sp. strain JC1, Methylovorus sp. strain SS1, Methylobacterium sp. strain SY1, and Methylosinus trichosproium enzymes.

• Research in Plant Disease
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• v.12 no.2
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• pp.91-98
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• 2006

A filtrate powder, designated as KNF2022, produced from culture broth of Acinetobacter sp. KTB3 was tested for their inhibitory effects on Pepper mild mottle virus (PMMoV) infection to Nicotiana glutinosa or N. tabacum cv. Xanthi nc. When 1/100 dilution with distilled water was treated to the plants and PMMoV was inoculated, the inhibition was estimated to be 94.3 and 95.6%, respectively. The same concentrations of KNF2022 inhibited infections of Pepper mottle virus (PepMoV) and Cucumber mosaic virus (CMV) on Chenopodium amaranticolor by 97.1 and 92.5%, respectively. Duration of inhibitory activity of the filtrate powder from Acinetobacter sp. culture broth against PMMoV infection on N. glutinosa was maintained for 2 days at 80% inhibition level, however, the inhibitory effect was diminished from 4 days after treatment to 50% levels. To evaluate inhibitory effects on systemic host plants of the antiviral agent, symptom developments of PMMoV, PepMoV and CMV on KNF2022-treated pepper plants were investigated. Delayed symptom developments until 10 days after inoculation (DAI) were observed for all the three viruses when the viruses were inoculated individually, and these delayed symptom development effects were maintained until 30 DAI in case of PepMoV. Moreover, PepMoV was not detected by RT-PCR and ELISA until 30 DAI. These delayed symptom development effects were diminished in all combinations of three virus co-inoculations due to synergism of three viruses on symptom developments. Inhibitory effect of KNF2022 was verified under electron microscopic examinations using purified virus preparations. Particles of PMMoV and PepMoV were observed on specimens from 5 min after KNF2022 treatment, and the particle sizes were reached in the range of 200-250 nm and 400-600 nm, respectively. Furthermore, the viral particles were destructed and particle sizes were reached in the range of 100-150 nm and 300-500 nm, respectively, on 60 min after treatments. Reduction of local lesion numbers on N. tabacum cv. Xanthi nc and C. amaranticolor were accompanied with reduction of virus particle sizes. In the case of CMV destructed particle numbers were also increased according to incubation period after KNF2022 treatment and local lesions on C. amaranticolor were reduced.