• 한국수산과학회지
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• 제31권5호
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• pp.767-773
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• 1998

1996년 6월부터 10월까지 적조다발해역 마산만에서 C. polykrikoides 살조세균의 분포와 분리된 110 균주 중 살조능이 우수한 Micrococcus sp. LG-1의 살조특성을 요약하면 다음과 같다. 현장해수에서 C. polykrikoides 살조세균은 $10^2\~10^3$cells/ml의 범위로, C. polykrikoides 적조가 발생한 9월에 $4.8\times10^3$cells/ml로 가장 높았으며, 적조가 소멸하는 10월에 $2.0\times10^2$cells/ml로 감소하는 경향을 나타내었다. C. polykrikoides에 대한 Micrococcus sp. LG-1의 살조특성을 혼합배양을 통하여 조사하였다. 잠복기와 대수증식기의 C. polykrikoides의 배양액에 Micrococcus sp. LG-1을 접종한 결과 각각 6일과 5일 만에 사멸되어, 대수증식기의 C. polykrikoides가 빠르게 사멸되었다. 또한, Micro-coccus sp. LG-1의 개체수와 배양여과액의 농도에 비례하여 C. polyklikoides의 사멸효과가 높게 나타났으며, 배양여과액은 최종농도가 2.5\%$일 경우는 접종 후 5.5시, $5\%$에서는 3.5시간, 10과 $20\%$에서는 1.5시간 그리고 $30\%$에서는 1시간만에 모두 사멸하였다. Alexandrium tamarense, Prorocentrum micans, Scrippsiella trochoidea. ana Gymnodinium sanguineum, Cochlodinium polykrikoides의 5종의 적조원인 편모조류에 대한 Micrococcus sp. LG-1의 살조특이성은 C. polykrikoides에만 살조효과를 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.388-398
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• 1998

A bacterium which is resistant to both mercury and cadmium, and also capable of utilizing phenol as a carbon and energy source, was isolated from the Kumho River sediments near Kangchang Bridge, Taegu, Korea. The isolate was labeled Pseudomonas sp. KM10 and characterized. The bacteria grew in 4 mM $CdCl_2$and in $70{\mu}M$ $HgCl_2$. The bacteria efficiently removed over 90% of 1 g/l phenol within 30 h. In the presence of 1.250 g/l phenol, the growth of the microorganism was slightly retarded and the microorganism could not tolerate 1.5 g/l phenol. Curing of plasmid from the bacteria was carried out to generate a plasmidless strain. Subsequent experiments localized the genes for phenol degradation in plasmid and the genes for mercury resistance and cadmium resistance on the chromosome. Dot hybridization and Southern hybridization under low stringent conditions were performed to identify the DNA homology. These results showed significant homologies between the some sequence of the chromosome of Pseudomonas sp. KM10 and merR of Shigella flexneri R 100, and between the some sequence of the chromosome of Pseudomonas sp. KM10 and cadA of Staphylococcus aureus pI258. The mechanism of cadmium resistance was efflux, similar to that of S. aureus pI258 cadA, and the mechanism of mercury resistance was volatilization, similar to that of S. flexneri R100 mer.

• 자연과학논문집
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• 제8권1호
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• pp.33-39
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• 1995

Klebsiella sp. L-10을 N-methyl-N'-nitro-N''-nitrosoguanidine 과 ethylmethane sulfornate 및 자외선 등으로 돌연변이시켜 히아루론산 복합체의 수율이 친주보다 약 2.5배 증대된 NTG 50 변이주를 얻었다. NTG 50변이주를 이용한 히아루론산 복합체의 최적 생산배지 조성은 효모 추출물 0.1%, 트립톤 3%, 포도당 3%, $K_2HPO_4$ 와 $KH_2PO_4$ 각각 30nM 이었고 배양조건은 배지의 초기 pH 6.0-6.5, 배양온도 $37^{\circ}C$, 24시간의 진탕배양이었으며 이때 배양액 리터당 2900mg의 히아루론산 복합체가 생산되었다.

• Animal Systematics, Evolution and Diversity
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• 제23권1호
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• pp.35-38
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• 2007

Two new sponges, Euryspongia coreana n, sp, and E. regularis n, sp., collected from Hataedo, Ulleungdo and Chujado, Korea by SCUBA diving during the period from 2001 to 2005. Euryspongia coreana n. sp, is very close to E. lactea Row, 1911 and E. arenaria Bergquist, 1961 in skeletal structure, but primary and secondary fibres of the new species are thicker than those of E. lactea, E. arenaria is cored with mostly spicule fragments but E. coreana n. sp. is cored with mainly sand. E. regularis n. sp. is readily distinguished from other eurypsongian sponges. by its thickly encrusting growth form, yellowish ivory colour, and thickness of fibres. The bright yellow color and regular ladder-like skeletal structure are major features distinguishing this E. regularis n. sp. from other euryspongian sponges. Most species are brown, violet, red or orange in color except for E. lactea (milky white), E. arenaria (biscuit) and E. coreana n. sp. (yellowish ivory). Other euryspongian sponges have regularly or irregularly well developed secondary fibres, but E. regularis n. sp. has simple and regular secondary fibres.

• 생명과학회지
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• 제12권6호
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• pp.745-751
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• 2002

원유 분해능이 강력한 해양균주를 얻고자 유류오염 지역으로부터 crude oil을 탄소원으로 이용하는 수십 종을 분리하였다. 분리된 균주중 원유분해능 및 biosurfactant 생성능이 우수한 균주를 선별하여, 형태학적, 생화학적 및 생리학적 특성을 조사한 후 Bacizzus sp. LSC11로 동정하였다. Bacillus sp. LSC11 균주 배양액의 표면장력은 최저 32mN/m까지 감소되었다. Biosurfactant의 CMC값은 0.0035% (w/v) 인 것으로 나타났다. Bacillus sp. LSC11가 생산하는 biosurfactant의 유화활성은 대두유에서 최대였으며, 원유에서도 높은 편이였다. 유화안정성은 합성계면활성제와 비슷하거나 우수하였다.

• Animal Systematics, Evolution and Diversity
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• 제30권4호
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• pp.248-259
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• 2014

This paper describes eight new species of the genus Coscinoderma from Chuuk Island, the Federated States of Micronesia. This genus is characterized by very fine, meandering uncored secondary fibres. All the new Coscinoderma species are compared with eight other valid species from tropical regions. Coscinoderma folium n. sp. is characterized by its large thickly foliate shape, and cored primary fibres are easily found between secondary fibres in choanosome. Coscinoderma lacium n. sp. differs in having round conules and no cored primary fibres. Coscinoderma cavernosa n. sp. is distinguished from other species by its cavernous shape and long sharp conules. Coscinoderma wenoa n. sp. is characterized by long conules and primary fibres. Coscinoderma mappula n. sp. differs in having a thick sand crust in the choanosome. Coscinoderma bakusi n. sp. is characterized by having several oscules at the top of the sponge and color changed grey to dark brown in alcohol. Coscinoderma pollax n. sp. is characterized by having a small thumb shape. Coscinoderma truki n. sp. similar to C. pollax in shape but differs in cored primary fibres with many spicules.

• The Plant Pathology Journal
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• 제22권4호
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• pp.381-385
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• 2006

Antifungal activity of Korean and Chinese lichen-forming fungi(LFF) was evaluated against plant pathogenic fungi of Botryosphaeria dothidea, Botrytis cinerea, Diaporthe actinidiae, Pestalotiopsis longiseta, Pythium sp., Rhizoctonia solani, and Sclerotium cepivorum. The LFF were isolated from Cladonia scabriuscula, Melanelia sp., Nephromopsis asahinae, Nephromopsis pallescens, Parmelia laevior, Pertusaria sp., Ramalina conduplicans, Ramalina sinensis, Ramalina sp., Umbilicaria proboscidea and Vulpicida sp. with discharged spore method. The isolates were deposited in the herbarium of Korean Lichen Research Institute(KoLRI) in Sunchon National University. The LFF of Melanelia sp., P. laevior, Pertusaria sp., R. conduplican and Ramalina sp. exhibited strong antifungal activity against all of the pathogenic fungi examined. Among them, LFF of P. laevior showed more than 90% of inhibition in fungal hyphae growth, compared with control. The results imply that LFF can be served as a promising bioresource to develop novel biofungicides. Mass cultivation of the LFF is now under progress in laboratory conditions for chemical identification of antifungal substances.

• Preventive Nutrition and Food Science
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• 제17권3호
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• pp.217-222
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• 2012

This study was carried out to investigate the isolation and characterization of lactic acid bacteria (LAB) from naturally fermented sauce-type kimchi. Sauce-type kimchi was prepared with fresh, chopped ingredients (Korean cabbage, radish, garlic, ginger, green onion, and red pepper). The two isolated bacteria from sauce-type kimchi were identified as Pediococcus pentosaceus and Lactobacillus brevis by 16S rDNA sequencing and tentatively named Pediococcus sp. IJ-K1 and Lactobacillus sp. IJ-K2, respectively. Pediococcus sp. IJ-K1 was isolated from the early and middle fermentation stages of sauce-type kimchi whereas Lactobacillus sp. IJ-K2 was isolated from the late fermentation stage. The resistance of Pediococcus sp. IJ-K1 and Lactobacillus sp. IJ-K2 to artificial gastric and bile acids led to bacterial survival rates that were 100% and 84.21%, respectively.

• 생명과학회지
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• 제12권5호
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• pp.561-565
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• 2002

Pseudomonas sp. EL-041는 페놀로 적응된 활성오니로부터 분리되었으며, 페놀을 분해할 수 있는 동시에 trick-loroethylene(TCE)을 공동대사할 수 있는 세균이다. 본 연구에서는 Pseudomonas sp. EL-041에서 발현되는 trick-loroethylene 분해효소의 종류를 효소활성 측정 및 specific phenol oxygenase gene의 DNA sequencing을 통하여 조사하였으며, 그 결과 본 균주의 trichloroethylene 분해효소는 monooxygenase였으며, phenol hydroxylase로 추정되었다.

• The Plant Pathology Journal
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• 제21권3호
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• pp.221-228
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• 2005

A total of 179 isolates of Trichoderma spp. were collected from oyster mushroom substrates in Korea. On the basis of morphological and cultural characteristics, Trichoderma isolates were divided into seven groups, namely T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, referred to as Trichoderma sp. 1 and 2. The predominant species was Trichoderma sp. 2 (n=86) followed by Trichoderma sp. 1 (n=52). Trichoderma sp. 1 and 2 were morphologically distinct not only from the other species of Trichoderma reported but also from each other in the characteristics such as mycelial growth rate, colony appearance, shape of conidia and conidiophores and branching pattern of phialides, although branching pattern of phialides of Trichoderma sp. 1 was similar to that of T. harzianum. In virulence test, the degree for compost colonization of Trichoderma sp. 2 was significantly greater than that of the other Trichoderma species. Trichoderma sp. 2 was found to be the main cause of green mold disease in oyster mushroom production. More work including molecular characterization is needed to confirm the species of Trichoderma sp. 1 and 2.