The Journal of Natural Sciences (자연과학논문집)
- Volume 8 Issue 1
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- Pages.33-39
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- 1995
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- 1225-6196(pISSN)
Production of High Viscous Hyaluronic Acid Complex from Klebsiella sp. L-10 NTG 50
Klebsiella sp. L-10의 NTG 50 변이주에 의한 고점성 히아루론산 복합체의 생산
- Lee, Hyaung-Sook (Dept. of Genetic Engineering, Paichai University) ;
- Choi, Young-Jun (Dept. of Genetic Engineering, Paichai University) ;
- Lee, Jong-soo (Dept. of Genetic Engineering, Paichai University)
- Published : 1995.09.20
Abstract
Klebsiella sp. L-10 was treated with physical and chemical mutagens, and one of the NTG mutant which increased hyaluronic acid complex yield 2.5 folds was selected. The yield of hyaluronic acid complex from Klebsiella sp. L-10 NTG 50 mutant reached maximum level I the YPD medium containing 0.1% yeast extract, 3% Bacto-tryptone, 3% dextrose, each 30mM of
Klebsiella sp. L-10을 N-methyl-N'-nitro-N''-nitrosoguanidine 과 ethylmethane sulfornate 및 자외선 등으로 돌연변이시켜 히아루론산 복합체의 수율이 친주보다 약 2.5배 증대된 NTG 50 변이주를 얻었다. NTG 50변이주를 이용한 히아루론산 복합체의 최적 생산배지 조성은 효모 추출물 0.1%, 트립톤 3%, 포도당 3%,