The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Morphological and Cultural Characteristics of Trichoderma spp. Associated with Green Mold of Oyster Mushroom in Korea

  • Park, Myung-Soo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National Unversity) ;
  • Seo, Geon-Sik (Department of Industrial Crops, Korea National Agricultural College) ;
  • Lee, Kang-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae, Kyung-Sook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yu, Seung-Hun (Department of Applied Biology, College of Agriculture and Lkife Sciences, Chungnam National Unversity)
  • Published : 2005.09.01
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Abstract

A total of 179 isolates of Trichoderma spp. were collected from oyster mushroom substrates in Korea. On the basis of morphological and cultural characteristics, Trichoderma isolates were divided into seven groups, namely T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, referred to as Trichoderma sp. 1 and 2. The predominant species was Trichoderma sp. 2 (n=86) followed by Trichoderma sp. 1 (n=52). Trichoderma sp. 1 and 2 were morphologically distinct not only from the other species of Trichoderma reported but also from each other in the characteristics such as mycelial growth rate, colony appearance, shape of conidia and conidiophores and branching pattern of phialides, although branching pattern of phialides of Trichoderma sp. 1 was similar to that of T. harzianum. In virulence test, the degree for compost colonization of Trichoderma sp. 2 was significantly greater than that of the other Trichoderma species. Trichoderma sp. 2 was found to be the main cause of green mold disease in oyster mushroom production. More work including molecular characterization is needed to confirm the species of Trichoderma sp. 1 and 2.

Keywords

References

  1. Chet, I. and Inbar, J. 1994. Biological control of fungal pathogens. Appl. Biochem. Biotechnol. 48:37-43 https://doi.org/10.1007/BF02825358
  2. Choi, I. Y., Hong, S. B. and Yadav, M. C. 2003. Molecular and morphological characterization of green mold, Trichoderma spp. isolated from oyster mushrooms. Mycobiology 31:74-80 https://doi.org/10.4489/MYCO.2003.31.2.074
  3. Fletcher, J. T. 1990. Trichoderma and Penicillium disease of Agaricus bisporus. A literature review for the Horticultural Development Council. London: ADAS
  4. Gam, W. and Bissett, J. 1998. Morphology and identification of Trichoderma. In: Harman, G E. and Kubicek, C. P., eds. Trichoderma and Gliocladium Vol. 1. Basic biology, taxonomy and genetics. London: Taylor & Francis. pp. 3-34
  5. Goltapeh, E M. and Danesh, T. R. 2000. Studies on interaction between Trichoderma species and Agaricus bisporus mycelium. Science and Cultivation of Edible Fungi 661-666
  6. Grogan, H. M. and Gaze, R. H. 1995. Growth of Trichoderma harzianum in traditional and experimental compost. In: Elliot, T. J. eds. Mushroom science XIV, Vol. 2. Balkema, Rotterdam, pp. 653-660
  7. Hjeljord, L. and Tronsmo, A. 1998. Trichoderma and Gliocladium in biological control: an overview. In: Harman, G. E. and Kubicek, C. P., eds. Trichoderma and Gliocladium Vol. 2. Enzymes, Biological Control Commercial applications. London: Taylor & Francis. pp. 131-151
  8. Kubicek, C. P. and Penttila, M. E. 1998. Regulation of production of plant polysaccharide degrading enzyme by Trichoderma. In: Harman, G. E. and Kubicek, C. P., eds. Trichoderma and Gliocladium Vol. 2. Enzymes, Biological Control Commercial applications. London: Taylor & Francis. pp. 49-71
  9. Morris, E. and Doyle, O. 1995. A profile of Trichoderma species I-mushroom compost production. Mushroom Sci. 14:611-618
  10. Nirenberg, H. I. 1976. Untersuchungen uber die morphologische und biologische Differenzierung in der Fusarillm-Sektion Liseola. Mitt Biol Bundesanstalt fur Land-Forstw Berlin-Dahlem. 169:1-117
  11. Ospina-Giraldo, M. D., Royse, D. M., Thon, J. R., Chen, X. and Romaine, C. P. 1998. Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90:76-81 https://doi.org/10.2307/3761014
  12. Rinker, D. L. 1993. Trichoderma green mold: a seminar by Dr. Donald Betterley, Monterey Labs. Mushroom News 44:20-23
  13. Romaine, C. P., Royse, D. J., Wuest, P. J. and Beyer, D. M. 1996. Mushroom green mold: cause, edaphic factors and control. Mushroom News 44:20-23
  14. Samuels, G. J. 1996. Trichoderma: a review of biology and systematics of genus. Mycol. Res. 100:923-935 https://doi.org/10.1016/S0953-7562(96)80043-8
  15. Samuels, G. J., Dodd, S. L., Gams, W., Castleburry, L. A. and Petrini, O. 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94: 146-170 https://doi.org/10.2307/3761854
  16. Seaby, D. A. 1987. Infection of mushroom compost by Trichoderma species. Mushroom J. 179:355-361
  17. Seaby, D. A. 1996. Differentiation of Trichoderma taxa associated with mushroom production. Plant Pathol. 45:905-912 https://doi.org/10.1111/j.1365-3059.1996.tb02901.x
  18. Sivasithamparam, K. and Ghisalberti, E. L. 1998. Secondary metabolism in Trichoderma and Gliocladium. In: Harman, G. E. and Kubicek, C. P., eds. Trichoderma and Gliocladium Vol. 1. Basic biology, taxonomy and genetics. London: Taylor & Francis. pp. 139-191

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