• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.200-201
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.212-213
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• 2005

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.5
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• pp.980-983
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• 1999

The contents of sterols and stanols in vegetable oils and mist oil were analyzed by gas chromatography using a capillary column. The total sterol contents showed high values of 0.67~0.89g/100g in corn oil, rice bran oil, red pepper seed oil and sesame oil. Mist oil, a byproduct of soybean oil manufacture, was a suitable raw material for the production of stanol since it showed high sterol content (10.2g/100g). In the hydrogenation of sterol contained in mist oil using Pd catalyst, the effects saponification of oil were examined. The conversion of sterol to stanol was improved by a factor of 4~5 through saponification of oil, compared to the reaction without saponification.

• Proceedings of the Membrane Society of Korea Conference
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• 1994.04a
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• pp.24-25
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• 1994

한식간장은 콩을 주원료로 하여 콩속의 성분을 수용성단백질 및 아미노산으로 변화시켜만든 조미료로서, 콩으로 메주를 만든다음 20일 동안 배양해서 만든 종국(Seed)과 혼합후 발효시킴으로써 제조되며 발효공정을 통해 Total Nitrogen이 증가되게 된다. 이러한 한식간장은 탈지대두를 사용하는 양조간장과는 달리 메주를 쑤어서 만들기 때문에 탈지가 되지 않은 제조과정으로 인하여 여과 및 공정중 발생하는 잡물질의 제거가 어렵게 된다. 제조공정중 여과공정을 거치게 되는데 여과포를 사용한 압착공정으로 이루어지며 여과된 간장에는 발효에 사용된 균$\cdot$효모등이 $10^7$개/ml 수준으로 존재하여 저장안정성 및 품질보전을 위해 열처리 과정을 거치게 된다. 이 열처리가 끝난 간장은 방치$\cdot$냉각시켜 첨가제를 혼합한 후 포장된다. 냉각과정중 2차 침전물이 생성되기도 하며 멸균이 확실치 않거나 2차 오염등으로 인한 발효로 인해 $CO_2$ Gas가 발생하기도 하여 품질이 나빠지는 원인이 된다. 이러한 열살균방식은 에너지를 많이 소비하고 열살균시 간장성분으로 인한 Scale 형성등 문제점을 안고 있다. 또한 열살균을 통해 살균은 할 수 있지만 사균, 분해물질, 간장에 함유된 고약한 냄새를 풍기는 잡성분의 제거는 불가능하다.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.531-535
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• 1998

This study was conducted to test the effect of chitosan, grape fruit seed extracts(GFSE) and sodium hypochloride gas on the control of mycotoxin producing pathogens occurred kin postharvest grains. Among the treatments, sodium hypochloride gas showed the highest control effect on wheat, soybean and corn see maintained in natural conditions after postharvest and GFSE had a little control effect, but chitosan treatment had no effect. Sodium hypochloride gas exhibited the strongest control effect on the major mycotoxin producing pathogens such as Penicillium spp. Aspergillus spp. and Fusarium spp., whereas GFSE had a little control effect. Sodium hypochloride gas appeared to be effective when the grains were treated with this gas more than 24 hours.

• Plant Resources
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• v.8 no.2
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• pp.116-121
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• 2005

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous or methanol extracts and plant residues from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine its allelopathic effects, and the results showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Aqueous leaf extracts of $40g\;L^{-1}$ were completely inhibitory on root growth of alfalfa, while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Leaf residue incorporation at $100g\;kg^{-1}$ into soil on seedling growth of barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) inhibited both shoot and root fresh weights of barnyard grass by 94 and $96\%$, respectively. Methanol extracts from BuOn and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did those from hexane and water fractions. The results based on bioassay of extracts and residues show that X. occidentale had potent an allelopathic activity against other plant species.

• YAKHAK HOEJI
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• v.27 no.2
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• pp.169-176
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• 1983

The verification of genuine sesame oil can be examined by determination of the ratio of fatty acid. Fatty acids were extracted from the saponifiable substance of sesame oils. Fatty acids were methylated with the 14% boron trifluoride methanol solution and injected into a gas chromatograph with Unisole 3000 column and finally determined the molecular weight by mass spectrometry. The fatty acids in laboratory prepared sesame oils were composed mainly of oleic acid 36.7-42.8% and linoleic acid 39.0-46.6%, including palmitic acid 7.9-9.l%, stearic acid 4.1-5.6%, linoleic acid 0.1-3.0%, arachidic acid 0.5-1.0% and eicosenoic acid 0.1-0.5%. The above results allow the estimation of genuine sesame oil, mixed with rape seed oil, soybean oil, perilla oil, etc. In 53 samples, 14 samples were estimated as genuine and it was found that erucic acid was contained in 31 samples, linoeic acid was highly contained in 14, high quantity of linolenic acid was in 7 and palmitic and oleic acid were highly involved in 3.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.338-342
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• 2015

There has been known C3G (Cyanidin-3-Glucoside), D3G (Delphinidin-3-Glucoside), and Pt3G (Petunidin-3-Glucoside) were main anthocyanin pigments in black-seeded soybean. Anthocyanin contents of total 1,032 black-seeded soybean germplasms were analyzed by HPLC. Average of total anthocyanin content was 11.67 mg/g on the all materials ranged from 0.54 to 23.45 mg/g. Mean value of C3G, D3G, and Pt3G contents in all black-seeded soybeans were 8.81, 1.78 and 0.79 mg/g, respectively. Environmental conditions influenced anthocyanin contents during seed development. Delayed flowering, especially of later maturing germplasms, has been showed to result in increased anthocyanin content. So, prolonged maturation period germplasm is generally higher than that of shorten genotypes. It may be concluded that the higher levels of anthocyanin content was associated with the late dates of harvest maturity. Also larger seeds showed high anthocyanin contents than smaller. That inclination is similar in C3G's occasion because C3G content contribute highly to total anthocyanin content than other pigments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.416-423
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• 1997

To obtain the basic information about the translocation of seed reserves of soybean[Glycine max(L.) Merrill] cultivars, 'Hill', 'Paldalkong' and 'Jangyeobkong' , the dry matter changes of emerging organs during the germination and seedling development were observed at the research farm of College of Natural Resources, Korea University, on May 26, June 5 and June 14. Mean fresh weight, dry weight and moisture content of three soybean cultivars were increased until the V2 stage in all seedling parts except cotyledons. Cotyledon dry weight was continuously decreased and the decreased amount at the V2 stage was about 80% of the cotyledon dry weight at the germination stage. Structural component of cotyledons was 22.2% of the cotyledon dry weight at the germination stage. Metabolic components of cotyledons were markedly decreased until V2 stage, and about 91% of cotyledon metabolic components at germination stage was utilized. However, those of whole seedling were increased after the V1 stage. Therefore, it appeared that role of cotyledons as nutrient supplier for germination and seedling growth was important until the V1 stage especially.

• Applied Biological Chemistry
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• v.45 no.4
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• pp.185-189
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• 2002

Along with the worldwide rapid increase of the cultivation area and commercial production of genetically modified (GM) crops, the amount of GM grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-photphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of EPSPS transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conducted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.