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Detection Methods for Genetically Modified Soybeans  

Sohn, Seong-Han (Division of plant Biotechnology, National Institute of Agricultural Biotechnology)
Jeong, Soon-Il (Experiment and Research Institute, National Agricultural Products Quality Management Service)
Yoon, Mun-Sup (Division of Genetic Resources, National Institute of Agricultural Biotechnology)
Kim, Tae-San (Division of plant Biotechnology, National Institute of Agricultural Biotechnology)
Park, Yong-Hwan (Division of plant Biotechnology, National Institute of Agricultural Biotechnology)
Kim, Young-Mi (Division of plant Biotechnology, National Institute of Agricultural Biotechnology)
Publication Information
Applied Biological Chemistry / v.45, no.4, 2002 , pp. 185-189 More about this Journal
Abstract
Along with the worldwide rapid increase of the cultivation area and commercial production of genetically modified (GM) crops, the amount of GM grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-photphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of EPSPS transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conducted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.
Keywords
genetically modified soybean; herbicide-tolerance; glyphosate; bioassay; PCR; qualitative analysis;
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