• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.51 no.1
• /
• pp.81-88
• /
• 2006

The 117 soybean cultivars were collected from nine provinces in Korea, and various seed quality traits along with isoflavone contents were evaluated to elucidate their relationship. The 100-seed weight of the black soybean (31.2 g) was significantly higher (p<0.05) than yellow soybeans (28.6 g). The composition of genistein, daidzein, and glycitein accounted for 75.8, 22.8, and 1.4 % of total isoflavone in yellow soybean cultivars, while their compositions in black soybeans were 58.5, 39.7, and 1.8%, respectively. The mean contents of total isoflavone in yellow and black soybean were $l,561.6{\mu}g\;g^{-1}\;and\;l,018.3{\mu}g\;g^{-1}$. The isofalvone content showed significant variation among cultivars when classified by the seed size. In the yellow soybeans, total isoflavone content was higher in small size soybean cultivars $(1,776.0{\mu}g\;g^{-1})$ and medium size soybean cultivars $(1,714.3{\mu}g\;g^{-1})$ compared to large size ones $(1,518.5{\mu}g\;g^{-1})$. Genistein content was proved as the major factor determining the relationship between isoflavone content and 100-seed weights (r =-0.206*). Daidzein and glycitein, however, showed no significant relationship with the 100-seed weights. Isoflavone content was not significantly correlated with color parameters L (lightness) and a (redness) values, but color parameter b (yellowness) was positively correlated with glycitein (r=0.264*) in the yellow soybeans, while its negative correlation between daidzein (r=-0.245*) and total isoflavone (r=-0.256*) were observed in black soybeans. However, these findings suggested that the seed color value may not serve as an effective parameter for estimating the isoflavone intensity of the soybeans. Variation of protein and lipid contents between yellow soybeans (n=58) and black soybeans (n=59) was relatively stable, however, protein and lipid contents have no significant relationship with isoflavone content.

• Journal of Life Science
• /
• v.16 no.6
• /
• pp.949-954
• /
• 2006

Soybean [Glycine max (L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvement of the quality and quantity of soybean seed constituents is one of the most important objectives in soybean breeding. Protein content and seed size are important properties to determine the quality of tofu and soy sprouts respectively. The objective of this study was to identify quantitative trait loci (QTLs) that control seed weight, protein and oil content in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RlL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.8, 0.78 and 0.71, respectively. Four independent QTLs for seed weight were identified from linkage group (LG) F, I and K. Five QTL for protein content were located on LG D1b, E, H, I and L. Oil content was related with six QTLs located on LG D1b, E, G, I, J and N. Protein and oil content have three common QTLs on LG D1b, E and I. Thus, we identified major loci improving soybean seed quality.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.2
• /
• pp.158-164
• /
• 1994

These experiment were conducted to evaluate the environmentally acceptable polymers, and 10 polymers were used in these study, and to investigate conductivity, germination percentage, water uptake of polymeric coating soybean seed. The conductivity of polymeric coating seed is higher than that of none coating seed and the highest conductivity was obtained with waterlock coating seed among the 10 polymer coating seed. As the soaking time was long, the conductivity was increased. The conductivity of large seed was higher than that of small seed, and that of long period storage seed was higher than that of short period storage seed. The effects of seed coating polymers on uptake water were various, and daran 8600 inhibited uptake water of low quality seed. The waterlock, captan, klucel and sacrust was rised germination percentage, and daran 8600 was declined germination percentage, and the effect of coating polymers on germination percentage of low quality seed was higher than that of high quality seed.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.28 no.3
• /
• pp.351-357
• /
• 1983

To study the relationships between soybean seed yield and meteorological elements, the investigation into the important agronomic characters of Bongeui cultivar and climatic factors such as precipitation, rainy days, and temperature from 1970 to 1981 was made. The results obtained were summarized as follows: 1) Coefficients of variabilities for pod-filling rate, seed yield, number of pods per plant, and seed weight were about 38%, 30%, 30%, and 5.5%, respectively. 2) Weather conditions mainly in July and August influenced soybean production. Particularly, high temperature played an important role in soybean yield reduction. 3) Correlation coefficients between maximum, minimum, or average temperature of July (and, in the case of August, maximum temperature only) and yield or seed weight, and between those of August and podfilling rate were significantly negative. 4) Regression equations between average temperature of July or maximum temperature of August and yield were Y=-42.46X +1200.86 and Y=-37.95X + 1210.42, respectively. 5) High temperature during the flowering stage affected soybean seed yield reduction significantly.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.38 no.4
• /
• pp.336-342
• /
• 1993

To obtain the basic information necessary for the development of soybean varieties well adaptable to upland-paddy field rotational croppings, the difference of growth characteristics between upland and paddy-field including yield potentials of current recommending soybean varieties were evaluated. The growth characteristics, both above and under-ground, which were measured at flowering stage were generally greater in paddy-field but the number of root nodules was much greater in upland, thus the artificial inoculation was practically recommended for soybean growing in paddy-fields. Mean seed yield was generally higher in paddy-fields than in upland. All soybean varieties showed higher seed yield in the early planting date, April 20, were somewhat susceptible to soybean mosaic virus(SMV), thus they could be escaped from the disasterous endemic necrotic soybean mosaic virus(SMV-N). Soybean varieties showed over 4.0 tons/ha seed yield in the paddy-field were Williams 79, Union, SS77053, and Namhaekong. At the same time, Jangyeobkong and Danyeobkong were the most stable soybean varieties among the tested soybean varieties with less than 10% of coefficient variation values in all planting dates in paddy fields. Compared with Hwangkeumkong which is most widely being cultivated on farmer's fields, soybean varieties showed high yields in paddy-field were higher in plant height, less in the number of branches, and more in the number of nodes on main stem. At the same time, they had medium seed size which would bring the good germination and stands. Disease resistance especially for necrotic soybean mosaic virus was also one of the most decisive factors in seed yields for the early planted soybeans.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.43 no.2
• /
• pp.105-112
• /
• 1998

Seeds of medicinal soybean [Glycine max(L.) Merill] are characterized by a black seed coat, white stripe at hilum border, yellow cotyledon, and very small seed weight. Production of this medicinal soybean has recently increased as a consumption increased. The objective of this study was to evaluate the growth characteristics and seed yield of collected medicinal soybeans and to obtain basic information on production practices and breeding materials. The collected medicinal soybean lines were cultivated at three locations for two years. Twenty-seven lines were planted at the Research Farm, Korea University, Namyangju city, on May 23, and at the Research Farm, Kyungpook National University, Taegu, on May 20, 1995. In 1996 field experiments, forty-four lines were planted on May 25, at Research Farm, Korea University, and twenty-seven lines among those were planted on June 7 at the Research Farm of National Yeongnam Agricultural Experiment Station, Milyang city. The investigated lines had purple or white flower. Flowering and maturing dates were similar or later than those of the control cultivars. Branch number was greater for the investigated lines. One hundred-seed weight of the lines ranged from 8.5 to 15.0 g. Mean seed yields ranged from 1.54 to 2.89 MT/ha. Nine lines of the investigated medicinal soybeans showed higher yield capacity than the control cultivars. Further research should be done on the improvement of the production system and breeding program of medicinal soybeans.

• Journal of Plant Biotechnology
• /
• v.47 no.2
• /
• pp.118-123
• /
• 2020

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F₂ seeds were obtained. A 3:1 segregation ratio was observed during F₂ seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F₂ seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ²=2.87, P=0.5 - 0.1). From nine F₂ seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

• Journal of Life Science
• /
• v.8 no.4
• /
• pp.437-442
• /
• 1998

Seed and leaf size is the important morphological traits considered by plant breeder and is the important yield components in soybean. The objective of this research was to know the relationship between seed size and leaf size in 66 $F_2$ populations derived from half diallel mating system with 12 soybean strains, representing distinct seed and leaf size. The range of seed size for 12 parents used was 6.7 to 43.8 g/100 seed. Leaf width leaf length ranged 5.7 to 8.6 cm and 9.4 to 12.9 cm, respectively. Leaf width was highly correlated with leaf length with an R square of 0.754 in the $F_2$ generation. The $F_2$ regression` coefficient indicated that leaves were, on average 1.4 times greater length than in width . Leaf size (width) was highly correlated (r.0.91) with seed size (g/100 seed) in the $F_2$ generation with an R square of 0.833. Our results indicate postive correlation within seed and leaf size is common in $F_2$ segregating populations derived from crossing with soybean. The strong liner relationship we observed between leaf size and seed size in $F_2$ segragating population is useful in that in that indirect selection for a secondary character may be superior to direct selection for the primary character.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.5
• /
• pp.666-670
• /
• 2008

Lovastatin (Mevinolin, Monacolin K) is a well-known drug for the therapy of hypercholesterolemia. It is an important fungal secondary metabolite as it inhibits 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) which catalyzes a major rate-limiting step in the biosynthesis of cholesterol. Both soybeans and black soybeans with Aspergillus terreus ATCC 20542 mutant were used as the seed culture for the mass production of lovastatin. The production of lovastatin in soybean seed culture of Asp. terreus was twofold compared to that of black soybean seed culture. The effect of two different vessels (petri dish and Erlenmeyer flask) on lovastatin production was also studied. The production of lovastatin on petri dish was tenfold to that of Erlenmeyer flask. Furthermore, the most lovastatin production on rice bran was achieved when the soybean seed culture was treated by heat shock at $30^{\circ}C$ for 1 hour, representing 82% of HMG-CoA reductase inhibition in the koji extract. We estimated that the heat treated soybean seed culture could be a new method for the mass production of lovastatin.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.61 no.1
• /
• pp.57-63
• /
• 2016

Soyasaponin I, II, III and V contents were investigated in seed, cotyledons and sprouts of soybean (Glycine max (L.) Merill) subjected to germination over five days. High-performance liquid chromatography coupled with tandem mass spectrometric (HPLC-MS/MS) method was used to evaluate the content of soyasaponins. Soyasaponins contents were different according to the varieties. Germination of soybeans dramatically increased soyasaponin contents in soybean sprouts in a time-dependent manner. Cotyledons had a higher contents of soyasaponins compared to dried seed (p<0.05). After five days of germination, Soyasaponin I and II increased 10 times higher after germination. Soyasaponin I and II are major metabolites in cotyledons and hypocotyls. Soyasaponin III and V were also detected in seed and increased depended on the germination stage. Soyasaponin V was at its highest levels in the hypocotyl, almost 7 times higher than the initial content in soybean seeds. Therefore, the germination of soybean sprouts significantly increased soyasaponin content.