• Archives of Pharmacal Research
• /
• v.28 no.7
• /
• pp.816-822
• /
• 2005

Catharsius protease-2 (CPM-2) was isolated from the body of dung beetles, Catharsius molossus, using a three step purification process (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel blue). The purified CPM-2, having a molecular weight of 24 kDa, was assessed homogeneously by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CPM-2 was composed of X Val Gin Asp Phe Val Glu Glu lie Leu. CPM-2 was inactivated by $Cu^{2+}\;and\;Zn^{2+}$ and strongly inhibited by typical serine proteinase inhibitors such as TLCK, soybean trypsin inhibitor, aprotinin, benzamidine, and ${\alpha}_1$-antitrypsin. However, EDTA, EGTA, cysteine, $\beta$-mercaptoethanol, E64, and elastatinal had little effect on enzyme activity. In addition, antiplasmin and antithrombin III were not sensitive to CPM-2. Based on the results of a fibrinolytic activity test, CPM-2 readily cleaved $A{\alpha}-$ and $B{\beta}$-chains of fibrinogen and fibrin, and y-chain of fibrinogen more slowly. The nonspecific action of the enzyme resulted in extensive hydrolysis, releasing a variety of fibrinopeptides of fibrinogen and fibrin. Polyclonal antibodies of CPM-2 were reactive to the native form of antigen. The ELISA was applied to detect quantities, in nanograms, of the antigen in CPM-2 protein.

• Korean Journal of Microbiology
• /
• v.26 no.4
• /
• pp.305-311
• /
• 1988

The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168 $spr^{r}$ chromosome at the frequency of $1.0\times 10^{-5}-5.0\times 10^{-7}$ and the transconjugante were selected on the yeast extract-mannitol plate containing kanamycin ($50{\mu}$g/ml) and spectinomycin ($100{\mu}$g/ml) after 8-9 days incubation. All transconjugants we tested were found to contain Tn 5 DNA on their genome, which was confirmed by Southern hybridization experiments. R. japonicum RNa75, which had been selected through plant test, was found to be defective in symbiotic nitrogen fixing ability and the production of leghemoglobin in soybean nodules formed by the inoculation of this mutant. In addition, this mutant strain hardly developed nitrogenase activity asymbiotically in contrast with the wild type strain, indicating that some nitrogen fixing gene might be blocked in this strain and the production of leghemoglobin could be decreased by the interference in nitrogen fixing genes.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.24 no.6
• /
• pp.1010-1015
• /
• 1995

Extent of reduction in protein digestibility by Godulbaegi(lxeris sonchifolia H.) kimchi powder appeared to be related to the kimchi weight-to-protein weight ratio, the kind of protein sources, part of plants and fementation period. In vitro digestibilities of protein were significantly(p<0.05) reduced as the freeze dried kimchi powder weight-to-protein ration increased from 0.5 : 1.0 to 1.0 : 1.0 for all Godulbaegi samples and protein sources. Overall digestibility of protein sources in the presence of kimchi powder, the reduction ranked in the following order : soybean>casein>beef>squid. Lower(p<0.005) reduction occurred for each protein source when raw plant was exchanged for kimchi products. Some greater reduction of digestibility was noted in young plants and leaf samples than ripe or root samples. Trypsin inhibitor, which expressed as soybena trypsin inhibitor, was inversely related(r=0.8437) to in vitro protein digestibility of casein in the presence of Godulbaegi kimchi powder. More than three times of total polyphenols contained in leaves than in roots. Young leaves had 30% more total polyphenols(37.64mg/g sample) than that in ripe ones. Soaking in 5% NaCl solution for 24 hrs was markedly reduced in total polyphenols as 73% for leaves and 33% for roots. Remarkable reduction in total polyphenols was not checked during fermentation followed after soaking. Trypsin inhibitor content correlated well(r=0.8873) with total polyphenols in all of Godulbaegi samples.

• Applied Biological Chemistry
• /
• v.32 no.3
• /
• pp.255-264
• /
• 1989

The minimum concentrations of organic solvents inducing phytotoxicity were investigated after spraying or flooding solvent solutions to annual plants. In the foliar treatment of upland test, solvents with high-boiling point or low-polarity induced severe phytotoxicity. Especially, aromatic solvents induced severe phytotoxicity. The most sensitive plant to phytotoxicity was soybean. In the pre-emergence treatment of upland test, the solvents with high-boiling point only induced phytotoxicity at high concentration. The degree of phytotoxicity induced by foliar spray in the condition of paddy field was similiar to that of upland test, but in the pre-emergence test, the former was more severe than the latter. Among 6 treatments, phytotoxicity induced by solvents was most severe in the pre-emergence test under the condition of flooding paddy field. Generally, alcohols induced severe phytotoxicity, especially, ethanol at 0.001%.

• Korean Journal of Soil Science and Fertilizer
• /
• v.46 no.2
• /
• pp.81-86
• /
• 2013

For organic farming, green manure crops such as leguminous forages and barley have been broadly used to improve soil fertility and soil physical and chemical properties by repeatedly cutting and mulching them directly as winter crop in the field in the rotation. In this investigation we selected 78 agricultural farm corporations as well as individual organic farmhouses related to crop rotation from greenmanure crops to main crops in order to analyze the relationship of cropping system between main crops and green manure crops. The results showed that the green manure crops were divided into two groups as leguminous and nonleguminous crops, representing that those are limited to specific climate and farming systems of regions. Also the 10 or less green manure crops including sudangrass, hairyvetch, italian ryegrass, sorghun, buckwheat, oat, pea, rye, clover, and canola which belong to leguminous crops which are presently cultivated from the organic farmhouses within the rotational crop system. We also confirmed that the major main crops are sweet potato, soybean, corn, tobacco, spinach from usage frequency analyzed by NetMiner H 2.6 which was used to estimate the rotational cropping system among the green manure crops and main crops.

• Korean Journal of Soil Science and Fertilizer
• /
• v.46 no.2
• /
• pp.73-80
• /
• 2013

Due to the fact that possible risk associated with soil-crop-food chain transfer, metal contamination in croplands has become a major topic of wide concern. Accumulation of toxic metals in edible parts of crops grown in contaminated soils has been reported from number of crops including rice, soybean, wheat, maize, and vegetables. Therefore, in order to ensure food safety, measures are needed to be taken in mitigating metal pollution and subsequent uptake by crop plants. Present paper critically reviewed some of the cost effective remediation techniques used in minimizing metal uptake by crops grown in contaminated soils. Liming with different materials such as limestone ($CaCO_3$), burnt lime (CaO), slaked lime [$Ca(OH)_2$], dolomite [$CaMg(CO_3)_2$], and slag ($CaSiO_3$) has been widely used because they could elevate soil pH rendering metals less-bioavailable for plant uptake. Zn fertilization, use of organic amendments, crop rotation and water management are among the other techniques successfully employed in reducing metal uptake by crop plants. However, irrespectively the mitigating measure used, heterogeneous accumulation of metals in different crop species is often reported. The inconsistency might be attributed to the genetic makeup of the crops for selective uptake, their morphological characteristics, position of edible parts on the plants in respect of their distance from roots, crop management practices, the season and to the soil characteristics. However, a sound conclusion in this regard can only be made when more scientific evidence is available on case-specific researches, in particular from long-term field trials which included risks and benefits analysis also for various remediation practices.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.10 no.2
• /
• pp.110-114
• /
• 1990

Although corn is regarded as the most feasible forage corp, its relatively low content of protein is the critical we a kness for animal feeding. Many researches have been carried out to improve protein level in corn forage, however, there are no indicatable results but corn-legume intercropping. Plot test and proximate analysis were fullfill to compare dry matter yield and available nutrients of silage corn mono-culture system with those of corn-legume intercropping system of forage plant and silage. The MO culture system were observed by two stage of maturity, milk stage (Aug. 3), yellow stage (Aug. 24), and obtained following results. 1. Dry matter yields per 10 a at milk stage in corn mono-culture system was 596.2kg and corn-legume intercropping systems were 609.0 kg (corn-cowpea), 591.0 kg (corn-soybean) and 563.1 kg (corn-frenchbean), respectively. And comparable to them, 1508.9 kg (corn mono.), 1482.8 kg (corn-cowpea), 1482.6 kg (cornsoybean), 1379.1 kg (corn-frenchbean) were harvested at yellow stage. 2. The general trends of chemical composition by stages of maturity in corn mono-culture systems were higher than that of corn-legume intercropping system. 3. Crude protein content in corn-legume silages were significantly higher than corn mono-culture at yellow stage, except corn-frenchbean intercropping system. 4. Crude fiber content in corn-legume silage was higher than corn mono-culture silage harvested at yellow stage. 5. Consequently, corn-legume intercropping improved nutritional quality of silage than that of corn-monocultivated silage.

• Korean journal of applied entomology
• /
• v.60 no.3
• /
• pp.277-286
• /
• 2021

The natural enemy was identified as a parasitic wasp, Binodoxys communis Gahan. B. communis taxonomically belongs to the family braconidae, subfamily aphidinae, and genera binodoxys. It develops normally at 17.5 to 32.5℃, and not at 35℃. Depending on the temperature, the longest development period of B. communis was 21.9 days at 17.5℃, and shortest was 6.46 days at 32.5℃. The effective accumulated temperature was 175.44 DD for females as well as males; however, the developmental threshold temperature was 10.87℃ and 10.74℃ for females and males, respectively. This parasitic wasp was found to prefer cotton aphids and soybean aphids as its prey.

• Korean Journal of Environmental Agriculture
• /
• v.13 no.3
• /
• pp.241-250
• /
• 1994

This research was carried out to investigate how the growth of corn was affected by the heavy metal contents in soils near the old zinc-mining sites, by analyzing correlation between the growth of corn and heavy metal contents in soils collected from Yonghari Ilwoulmyun YongyangGun in Kyeongpook province in 1993. The results obtained were summarized as follows. 1. The contents of heavy metals such as Zn, Cu, Pb, Cd, and as in the Youngyang area were very high compared with those in a normal area. Heavy metal contents in soils collected from $2.0{\sim}2.5Km$ distance from the mining area were the highest, and those from 3.0 Km than those from 1.5 Km were even higher. 2. For heavy metal contents in leaves of all surveyed crops, Zn, Pb, Cu and As were the highest in soybean, followed by corn and rice. 3. Growth parameters of corn in polluted fields were comparatively poor and heavy metal contents in soils of the respective sites were higher than those in fields where rice was cultivated 8 years ago and irrigation was not done previously. 4. Heavy metal contents in the leaf part of corn plant showed a similar tendency to those in soils, being the highest among the different parts of corn plant, and they were in the decreasing order of Zn > Cu > As > Cd > Pb in each part. But the differences of metal contents in each part varied. 5. Generally, a negative relationship existed between the growth of corn and heavy metal contents in soil, of which Cu and Pb were significantly correlated with plant height, ear height, diameter of stem, ear length and yield of corn. 6. There existed a positive correlation between the contents of Pb,Cd and As in soils and those in the different parts of corn plant. The higher contents of Pb, Cd, and As in soil, the more those in corn plant incressed. The contents of Pb and As in corn grains showed a highly significant positive correlation with Cd and As contents in soils.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.29 no.4
• /
• pp.691-700
• /
• 2000

The purpose of this study was to estimate food intakes of the long-lived elderly and to obtain the data for establishing dietary guidelines that may be recommended for the general population for the sake of longvity. The subjects of the study were 224 elderly people of age over 85 years living in Kyungpook Sung-Ju area who have no problem in daily living. The food consumption survey was carried out seasonally by the repeated 24-hr recall method for one year. The subject group for this study was composed of 58 males and 166 females, the average age being 87 years old. Food intakes of the winter were more than any other seasons. The mean daily total food intake per capita was 594.4g, 513.5 g (86.4%) from plant foods and 79.3 g(13.3%) from animal foods. The sequence of high intakes of food groups were cereals, vegetables, fruits, fishes, legumes and meats. Boiled white rice, beef soup, soybean paste soup, soybean paste stew, broiled yellow croaker, kimchi, ra myon and broiled noodles were consumed most frequently. In conclusion, the subjects consumed much more plants foods than animal foods and consumed more natural and seasonal foods than processed foods and also showed food habits of eating small meals. Dietary habits of eating small meals containing abundant amount of fresh plant foods might partially contribute to the longevity of the subjects.