• Asian-Australasian Journal of Animal Sciences
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• v.32 no.11
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• pp.1725-1733
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• 2019

Objective: Evaluate the partial replacement of soybean meal with different protein sources in piglet feed during the nursery phase in terms of digestibility of feed, nitrogen balance, growth performance and blood parameters. Methods: Experiment I involved 24 crossbred entire male pigs with an initial body weight (BW) of $18.28{\pm}0.7kg$ and used a randomized complete block design consisting of 3 treatments (fish meal, FM; soybean protein concentrate, SPC; and soybean meal, SBM) and 8 replicates, with 1 pig per experimental unit. Experiment II involved 1,843 crossbred male and female pigs with an initial BW of $6.79{\pm}0.90kg$ and was based on a completely randomized design with a $2{\times}3$ factorial arrangement (2 sexes and 3 protein sources) and 13 replicates. Results: The results of Exp. I indicate effects (p<0.05) of dietary protein sources on digestible protein (FM, 17.84%; SPC, 16.72%, and SBM, 18.13%) and on total nitrogen excretion (TNE, $g/kg\;BW^{0.75}/d$) in which pigs fed with SBM-based feed had TNE values that were 5.36% and 3.72% greater than SPC and FM, respectively. In the Exp. II, there was difference (p<0.01) between sexes in the pre-starter I and starter phases, and total period in average daily feed intake (ADFI), which were greater in females, and between the protein sources, ADFI, final weight and daily weight gain. For urea in the pre-starter II and starter phases and glucose in the pre-starter II phase, there was a difference (p<0.05) between protein sources and between sexes, in starter phase in urea concentrations (females: 57.11 mg/dL and males: 50.60 mg/dL). Conclusion: The use of SBM as only protein source influences larger TNE ($g/kg\;BW^{0.75}/d$), reduces the growth performance of piglets and increases plasma urea concentrations in prestarter II phase.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2000.11a
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• pp.97-99
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• 2000

Considerable beneficial effects on osteoporosis from soy intake in postmenopausal women are being reported (Murkies et al.1998, Arjmandi et al. 1996). Isoflavones(IF), one of phytoestrogenic substances in the soybean, have been suggested for the effect. Our preliminary study showed that even the young chicks fed IF-rich diet tended to have higher bone ash content than those fed IF-poor diet. IF have been reported to decrease bone ash content than those fed IF-poor diet, IF have been reported to decrease bone loss by reducing bone resorption. Soybean meal has been one of the most important protein sources in poultry diets. We assume that the IF intake through dietary soybean meal could give meaningful influences on the birds. This study was carried out to determine effects of dietary IF from soybean sources on bone development in young chicks. Soy protein concentrate (SPC, IF-poor)and an IF concentrate(Phyto-Nutramin) were formulated together with purified-type ingredients to provide three different levels(25, 240 and 480 mg/kg) of total IF. Control diet(240 mg/kg) was prepared with isolated soyprotein(ISP, IF-rich). The diets were fed to 96, 3-day old, layer-type, male chicks, divided into 4 treatments with 3 replications for 3 weeks. Chicks fed the ISP diet had better gain/feed ratios than fed the SPC diets. Birds fed the diets with higher levels of IF tended to show higher values in serum total and ionized Ca% and tibial bone density, length % ash, stiffness and strain. This trend however, appeared less significant at the end of third week. No noticeable differences in sizes of comb and testicle and serum alkaline phosphatase activities were observed among the dietary groups. These results suggest that dietary isoflavones from soybean sources could be associated with chemical composition and physical properties of bone in sizes of comb and testicle and serum alkaline phosphatase activities were observed among the dietary groups. These results suggest that dietary isoflavones from soybean sources could be associated with chemical composition and physical properties of bone in young chicks.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.4
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• pp.519-529
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• 2015

The aims of this study were to determine the effects of feeding medium-roasted soybean meal (SBM) and corn dried distiller's grains with solubles (CDDGS) in dairy cows on milk production and income over feed costs. A randomized complete block design experiment was conducted with 24 crossbred multiparous Holstein Friesian dairy cows in early- and mid-lactation. Four dietary treatments were as follows: basal diet without feed substitute (Control), 7.17% dry matter (DM) roasted SBM replaced for concentrate (R-SBM), 11.50% DM CDDGS replaced for concentrate (DDGS), and 3.58% DM roasted SBM plus 5.75% DM CDDGS replaced for concentrate (SB-DG). The roasted SBM was produced using a medium-heated treatment at $100^{\circ}C$ for 180 min. Dry matter intake was not affected by feeding high rumen undegradable protein (RUP) sources, but the replacement of roasted SBM and CDDGS for concentrate significantly improved (p<0.001) RUP intake (0.90, 0.86, and 0.88 kg/d corresponding to R-SBM, DDGS, and SB-DG) compared to the control (0.61 kg/d). Feeding roasted SBM and CDDGS alone or in combination had no significant effect on milk composition of dairy cows (p>0.05), whereas milk yield was significantly increased by 3.08 kg/d in the SB-DG group relative to the control group (p<0.01). Net income was meaningfully increased (p<0.05) from 4th week post feeding, the SB-DG group reached the greatest net income ($3.48/head/d) while the control group had the lowest value ($2.60/head/d). In conclusion, the use of CDDGS alone or in combination with medium-roasted SBM as substitute for concentrate in lactating dairy cattle diet led to improved milk production and net income over feed costs without affecting total dry matter intake and milk composition, while feeding medium-roasted SBM seemed to show intermediate values in almost parameters.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.5
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• pp.598-606
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• 2010

This study investigated the nutritional quality of soybean meal (SBM) fermented by Aspergillus ($FSBM_A$) and/or followed by Lactobacillus fermentation ($FSBM_{A+L}$). Both fermented products significantly improved protein utilization of SBM with higher trichloroacetic acid (TCA) soluble true protein content, in vitro protein digestibility and available lysine content, especially in $FSBM_{A+L}$. Moreover, $FSBM_{A+L}$ produced a huge amount of lactic acid resulting in lower pH as compared to the unfermented SBM or soybean protein concentrate (SPC) (p<0.05). $FSBM_A$ and $FSBM_{A+L}$ raised 4.14% and 9.04% of essential amino acids and 5.38% and 9.37% of non-essential amino acids content, respectively. The ${\alpha}$-galactoside linkage oligosaccharides such as raffinose and stachyose content in $FSBM_A$ and $FSBM_{A+L}$ decreased significantly. The results of soluble protein fractions and distribution showed that the ratio of small protein fractions (<16 kDa) were 42.6% and 63.5% for $FSBM_A$ and $FSBM_{A+L}$, respectively, as compared to 7.2% for SBM, where the ratio of large size fractions (>55 kDa, mainly ${\beta}$-conglycinin) decreased to 9.4%, 5.4% and increased to 38.8%, respectively. There were no significant differences in ileal protein digestibility regardless of treatment groups. SPC inclusion in the diet showed a better protein digestibility than the SBM diet. In summary, soybean meal fermented by Aspergillus, especially through the consequent Lactobacillus fermentation, could increase the nutritional value as compared with unfermented SBM and is compatible with SPC.

• Animal Bioscience
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• v.36 no.9
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• pp.1384-1392
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• 2023

Objective: Replacing soybean meal (SBM) with cricket (Gryllus bimaculatus) meal pellets (CMP) in concentrate diets was investigated for feed efficiency, ruminal fermentation and microbial protein synthesis in Thai native beef cattle. Methods: Four male beef cattle were randomly assigned to treatments using a 4×4 Latin square design with four levels of SBM replaced by CMP at 0%, 33%, 67%, and 100% in concentrate diets. Results: Results revealed that replacement of SBM with CMP did not affect dry matter (DM) consumption, while digestibilities of crude protein, acid detergent fiber and neutral detergent fiber were significantly enhanced (p<0.05) but did not alter digestibility of DM and organic matter. Increasing levels of CMP up to 100% in concentrate diets increased ruminal ammoniacal nitrogen (NH₃-N) concentrations, blood urea nitrogen, total volatile fatty acids and propionate concentration (p<0.05), whereas production of methane and protozoal populations decreased (p<0.05). Efficiency of microbial nitrogen protein synthesis increased when SBM was replaced with CMP. Conclusion: Substitution of SBM with CMP in the feed concentrate mixture at up to 100% resulted in enhanced nutrient digestibility and rumen fermentation efficiency, with increased volatile fatty acids production, especially propionate and microbial protein synthesis, while decreasing protozoal populations and mitigating rumen methane production in Thai native beef cattle fed a rice straw-based diet.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.9
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• pp.1285-1293
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• 2005

The present study was conducted to evaluate and compare the effects of various animal and plant protein sources on piglet' performance, digestibility of amino acids and gut morphology in weaned pigs until 28 days after weaning. The plant protein sources used were soybean meal (SBM), fermented soy protein (FSP), rice protein concentrate (RPC); and animal protein sources tested were, whey protein concentrate (WPC) and fishmeal (FM). Iso-proteinous (21%) diets were formulated and lysine (1.55%) content was similar in all the diets. The level of each protein source added was 6% by replacing SBM to the same extent from the control diet containing 15% SBM. The ADG was higher (p<0.05) in the groups fed animal proteins as compared with plant proteins at all the levels of measurement, except during 15-28 days. The highest ADG was noted in WPC and FM fed diets and lowest in SBM fed diet. The feed intake was higher in animal protein fed groups than plant proteins at all phases, but the feed:gain ratio was not affected by protein sources except during overall (0 to 14 day) measurement which was improved (p<0.05) in animal protein fed diets compared to plant protein sources. The digestibilities of gross energy, dry matter and crude protein were higher in animal protein fed groups than for plant protein fed sources. The apparent ileal digestibilities of essential amino acids like Leu, Thr, and Met were significantly (p<0.05) higher in animal proteins fed animals as compared with plant protein fed animals. But the apparent fecal digestibilities of essential amino acids like Arg and Ile were significantly higher (p<0.05) in plant protein diets than animal protein sources. The villous structure studied by scanning electron microscope were prominent, straight finger-like, although shortened and densely located in FM fed group as compared with others. The lactic acid bacteria and C. perfringens counts were higher in caecal contents of pigs fed plant proteins than the animal proteins. Overall, it could be concluded that animal protein sources in the present study showed better effects on growth performance, nutrient digestibility and gut morphology than plant protein sources.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.1
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• pp.44-49
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• 2003

Effects of supplementation with ruminal epithelial cells on fiber-degrading activity and cell growth of Ruminococcus albus (R. albus, strain 7) was tested using a basal substrate of rice straw and formulated concentrate. Cultures of R. albus alone and R. albus with rumen protozoa were grown at $39^{\circ}C$ for 48 h with an 8.4% crude protein (CP) substrate, 33% of the CP supplemented with either ruminal epithelial cells or defatted soybean meal. The ruminal epithelial cells had lower amounts of rumen soluble and degradable protein fractions as compared to defatted soybean meal, as determined by an enzymatic method, and the same was found with amino acid composition of protein hydrolysates. Ruminal epithelial cells were directly utilized by the R. albus, and resulted in greater growth of cell-wall free bacteria compared to defatted soybean meal. The effect of epithelial cells on bacterial growth was enhanced by the presence of rumen protozoa. In consistency with cultures of R. albus and R. albus with rumen protozoa, fermentative parameters such as dry matter degradability and total volatile fatty acid did not differ between supplementation with ruminal epithelial cells or defatted soybean meal.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.1004-1009
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• 2006

Four male crossbred native beef cattle (average body weight of 427.7 kg) were randomly allocated to four types of cassoy-urea pellet as a source of protein in concentrate according to a $4{\times}4$ Latin square design to determine effect of diets on ruminal fermentation and nutrient digestibility. The four types of cassoy-urea pellets contained cassava hay, soybean meal, urea and binding agent at 79.2:19.8:0:1 (27.9% CP dry matter), 78.4:19.6:1:1 (30.4% CP), 77.6:19.4:2:1 (33.0% CP) and 99:0:0:1 (23.8% CP) for dietary treatments; 1, 2, 3 and 4, respectively. All four concentrate mixtures contained similar crude protein levels (11% CP) and were fed to animals in two equal parts (0.5% of body weight per day) while urea-treated rice straw (5% urea) was given ad libitum. The experiment revealed that dietary concentrate treatments had no effect on dry matter intake while digestibilities of neutral-detergent fiber and crude protein were higher (p<0.05) in cattle fed dietary treatments 1, 2 and 3 than in cattle fed dietary treatment 4. Ruminal ammonia-nitrogen ($NH_3$-N), was higher and acetic acid concentration (C2) and ratio of C2 to propionic acid (C3) were lower (p<0.05) in cattle fed dietary treatments 1, 2 and 3 than in those on treatment 4. It is concluded that use of cassoy-urea pellet as a protein source in concentrates for cattle resulted in improvement of digestibility, ruminal fermentation and rumen ecology. Further research using cassoy-urea pellet in feeding trials with milking cows and fattening beef should be undertaken.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.4
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• pp.374-381
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• 2019

This study was conducted to examine a mixture of plant protein sources as a fish meal (FM) substitute. Two feeding trials were carried out using similar dietary formulations but different FM levels. In Experiments 1 and 2, the basal diets were formulated to contain 65% and 60% of FM, respectively. The other five diets were formulated replacing FM by 10, 15, 20, 25 and 30% with a mixture of soybean meal, wheat gluten and soy protein concentrate. Three synthetic amino acids (lysine, threonine and methionine) were added to the test diet. Groups of fish in experiment 1 ($6.76{\pm}0.03g$) and experiment 2 ($32.5{\pm}0.1g$) were fed one of the experimental diets for 7 and 9 weeks, respectively. Each experiment was carried out in triplicate. There were no significant differences among groups in terms of growth performance, feed utilization, survival or hematological parameters in either experiment. The results indicated that a mixture of soybean meal, wheat gluten and soy protein concentrate, supplemented with three synthetic amino acid, can replace fish meal by up to 30% in diets for juvenile olive flounder.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.11
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• pp.1598-1604
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• 2001

Eight $12.4{\pm}0.6kg$ initial body weight crossbred barrows were used to determine the effect of soybean galactooligosaccharides on nutrient and energy digestibility, and digesta transit time. Four dietary treatments were utilized in this trial. Treatment one was a corn-soybean meal based diet (SBM) containing raffinose and stachyose at the levels of 0.16% and 0.75%, respectively. Treatment two (control) was a corn-HP300 (soybean concentrate protein) diet. In treatments three and four, 1.1% and 2.2% commercial stachyose was added to the control diet to provide total dietary stachyose at the levels of 1% and 2%, respectively. The soybean galactooligosaccharides (raffinose + stachyose) level in treatment one was slightly lower compared to that in treatment three. Three collection periods were run with two pigs for each treatment/period. There was a 4 d adjustment period followed by a 3 d collection period. The results showed that the nitrogen retention (86.79%) of pigs fed treatment two diet was higher than that of pigs fed treatment one by 5.2% (p<0.05). The nitrogen retention of treatment three was intermediate 83.09%. The apparent fecal digestibility of all amino acids in treatment two was numerically highest, followed by treatments three and four. However, there were no significant difference among groups (p>0.05). The dry matter (DM), organic matter (OM), crude protein (CP), and crude fiber (CF) digestibility numerically decreased as the soybean galactooligosaccharides level increased, but were not significantly different (p>0.05). Chromium content in feces (from the inclusion of 0.3% chromic oxide in the diets) differed among treatments (p<0.05) at 15 h, 18 h, and 21 h after eating. This showed that the digesta transit time was differed significantly among treatments. Treatment four was the shortest, followed by treatment three, SBM and control. The results demonstrated that in the absence of antinutritional factors and soybean antigen protein, inclusion of 1% and 2% stachyose in corn-HP300 diet has no significant effect on the digestibility of DM, OM, CP, CF and amino acids. When the soybean galactooligosaccharide level in diet one and diet three were adjusted to be almost the same, antinutritional factors such as trypsin inhibitor and soybean antigen protein could decrease the nutrient digestibility and nitrogen retention rate of diet. High levels of soybean galactooligosaccharides shortened the digesta transit time in the intestinal tract. This trial suggested that the total level of soybean galactooligosaccharides (stachyose+raffinose) in the weanling piglet diet is better not to exceed 1% when common soybean meal is used as main protein source.