• Molecules and Cells
• /
• v.37 no.6
• /
• pp.480-486
• /
• 2014

Growth restriction by antibiotics is a common feature that pathogenic bacteria must overcome for survival. The struggle of bacteria to escape from growth restriction eventually results in development of antibiotic-resistance through the expression of a set of genes. Here we found that some physiologically important transcriptional regulators of Pseudomonas aeruginosa including QscR, a quorum sensing (QS) receptor, SoxR, a superoxide sensor-regulator, and AntR, a regulator of anthranilate-related secondary metabolism, are activated by various growth-restricted conditions. We generated the growth-restricted conditions by various methods, such as overexpression of PA2537 and treatment with antibiotics or disinfectants. The overexpression of PA2537, encoding an acyltransferase homologue, tightly restricted the growth of P. aeruginosa and significantly activated QscR during the growth restriction. Similarly, treatments with gentamycin, tetracycline, and ethanol also activated QscR near their minimal inhibitory concentrations (MICs). Some non-QS regulators, such as AntR and SoxR, were also activated near the MICs in the same conditions. However, LasR and PqsR, other QS receptors of P. aeruginosa, were not activated, suggesting that only a specific set of transcriptional regulators is activated by growth restriction. Since paraquat, a superoxide generator, significantly activated QscR and AntR, we suggest that the oxidative stress generated by growth restriction may be partly involved in this phenomenon.

• Animal Bioscience
• /
• v.37 no.4
• /
• pp.609-621
• /
• 2024

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

• Proceedings of the KIPE Conference
• /
• 2008.06a
• /
• pp.15-17
• /
• 2008

저온 플라즈마를 이용한 탈황탈질 시스템은 한 개의 반응기에서 오염물질을 동시에 제거함으로써 설비가 매우 compact하고 운전비가 저렴한 장점을 가지고 있다. 본 논문은 펄스 고전압 방전에 의한 저온플라즈마를 이용하여 Sox, Nox동시 제거를 위한 전원장치의 회로 구성과 전원장치의 동작특성을 설명하였다. 그리고 당사의 탈황탈질 시스템 기술현황 및 향후계획을 논의하였다.

• Proceedings of the KIPE Conference
• /
• 2008.06a
• /
• pp.157-159
• /
• 2008

Generally, Capacitor charging power supply (CCPS) consist of the full-bridge inverter, LC resonant tank and capacitance load. The multi-connection systems are normally used for high voltage or high power application. In this paper, conventional parallel operation resonant type and proposed series operation PWM type CCPS for pulse corona occurrence of DeSOx, DeNOx system using magnetic pulse compressor make a comparison. The effect of proposed CCPS is verified by circuit characteristics analysis of parallel operation resonant type and series operation PWM type, and it is confirmed by simulation result.

• Korean Journal of Microbiology
• /
• v.55 no.3
• /
• pp.309-312
• /
• 2019

Comamonas sp. NLF-7-7 was isolated from biofilter of wastewater treatment plant. The whole-genome sequence of Comamonas sp. NLF-7-7 was analyzed using the PacBio RS II and Illumina HiSeqXten platform. The genome comprises a 3,333,437 bp chromosome with a G + C content of 68.04%, 3,197 total genes, 9 rRNA genes, and 49 tRNA genes. This genome contained pollutants degradation and floc forming genes such as sulfur oxidization pathway (SoxY, SoxZ, SoxA, and SoxB) and floc forming pathway (EpsG, EpsE, EpsF, EpsG, EpsL, and glycosyltransferase), respectively. The Comamonas sp. NLF-7-7 can be used to the purification of wastewater.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2005.06a
• /
• pp.335-335
• /
• 2005

• Particle and aerosol research
• /
• v.15 no.1
• /
• pp.1-13
• /
• 2019

Current desulfurization and denitrification technologies have reached a considerable level in terms of reduction efficiency. However, when compared with the simultaneous reduction technology, the individual reduction technologies have issues such as economic disadvantages due to the difficulty to scale-up apparatus, secondary pollution from wastewater/waste during the treatment process, requirement of large facilities for post-treatment, and increased installation costs. Therefore, it is necessary to enable practical application of simultaneous SOx and NOx treatment technologies to remove two or more contaminants in one process. The present study analyzes a technology capable of maintaining simultaneous treatment of SOx and NOx even at low temperatures due to the electrochemically generated strong oxidation of the wet-pulse complex system. This system also reduces unreacted residual gas and secondary products through the wet scrubbing process. It addresses common problems of the existing fuel gas treatment methods such as SDR, SCR, and activated carbon adsorption (i.e., low treatment efficiency, expensive maintenance cost, large installation area, and energy loss). Experiments were performed with varying variables such as pulse voltage, reaction temperature, chemicals and additives ratios, liquid/gas ratio, structure of the aeration cleaning nozzle, and gas inlet concentration. The performance of individual and complex processes using the wet-pulse discharge reaction were analyzed and compared.

• Applied Chemistry for Engineering
• /
• v.7 no.2
• /
• pp.269-279
• /
• 1996

The porous armophous catalysts, composed of $SiO_2-TiO_2-V_2O_5$ were prepared through SOL-GEL process by differing the preparation parameters. Selected the optimum SOL-GEL catalyst which has the best NO removal activity through the screening test, and repeated coating it on the cordierite monolith which is the freely through and inert. And examined the NOx, SOx simultaneous removal effect under S. C. R. condition. In addition to its characterization performances by the DTA, TGA, BET, $NH_3$ and NO TPD, ESCA, XRD etc.

• Proceedings of the Zoological Society Korea Conference
• /
• 2000.10a
• /
• pp.59.3-60
• /
• 2000

No Abstract, See Full Text

• Proceedings of the KAIS Fall Conference
• /
• 2009.05a
• /
• pp.848-850
• /
• 2009

화석에너지 고갈로 인한 유가 상승으로 폐기물 에너지화가 이슈화 되고 있다. 폐기물 고형 연료 RDF는 에너지 문제를 해결하기위한 대안 중 하나로 수송성, 저장성, 연소안정성이 우수하나 환경오염 물질의 발생이 문제가 된다. 이러한 오염물질을 처리하기 위한 배가스 오염 물질처리를 위한 plant 설비 비용과 시간이 많이 투자된다. Aspen plus는 mass energy balance와 화학적평형, 열역학을 이용하여 공정 모사를 할 수 있는 프로그램으로 검증되었다. RDF의 삼성분, 원소분석, 발열량을 입력을 통해 HCl, SOx, NOx, CO의 배출량을 예측하고 이에 맞는 SNCR, SDA 등과 같은 반응기를 적용 하므로써 다양한 배가스 처리를 모사 할 수 있다. NOx를 제어하기위에 urea주입과 SOx와 HCl을 제거하기 위한 CaO를 주입을 모사 하므로써 실제 운영 적용 전 단계에 역할로 유용한 도구로 판단된다.