• Title/Summary/Keyword: Southern plants

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A Study of the Improvement Plan and Real Condition Estimation of Fire Protection Safety Management for Power Plants in Korea (국내발전소 소방안전관리 운영실태조사 및 개선방안에 관한 연구)

  • Kang, Gil-Soo;Choi, Jae-wook
    • Fire Science and Engineering
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    • v.31 no.2
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    • pp.61-73
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    • 2017
  • The Fukushima Nuclear Disaster in 2011 and California Power Failure in 2001 are examples of the importance of the power plant safety management that caused huge national loss with a power-related mass casualty incident. In a situation where humans cannot live without electricity, efforts to strengthen the systematic firefighting safety management in power plants that produce electricity with large amounts of hazardous materials as fuel, such as nuclear energy, coal and gas, are essential to protect life and prevent property loss and stable economic growth from fire explosion accident or radiation leak due to the negligence of safety management and natural disasters such as earthquakes, which has recently become an issue. This study examined the operating situation of firefighting safety management in power plants with firefighting officials employed by five power generation companies including Korea Southern Power Co., Ltd. and Korea Hydro & Nuclear Power Co. Ltd., which are in charge of the domestic power supply. As a result, for the systematic firefighting safety management of power plants, improvement plans were drawn, including the development of an effective business manual and a comprehensive management system, the substantiality of firefighting safety education, and the strengthening of seismic designs to prepare for earthquakes.

Soil Chemical Properties and Plant Mineral Contents in Plastic Film House in Southern Part of Korea (남부지방(南部地方) 시설재배지(施設栽培地) 토양의 화학성(化學性)과 작물의 양분함량(養分含量))

  • Ha, Ho-Sung;Yang, Min-Sug;Lee, Hyub;Lee, Yong-Bok;Sohn, Bo-Kyoon;Kang, Ui-Gum
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.3
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    • pp.272-279
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    • 1997
  • Chemical properties of soils and mineral contents in plants grown at seventy one regions located in southern part of Korea were surveyed to obtain the basic information for sustainable management of plastic film house soils. The averaged soil chemical properties showed optimum levels in pH, organic matter, available boron, manganese, zinc contents, whereas available phosphate and exchangeable rations were highly accumulated. Available phosphate showed highly positive correlation with continuous cropping period. The contents of phosphate, potassium, calcium, and magnesium in the top soils were ranged from 912(Pusan)~1.796mg (Masan) ; $1.52(Pusan){\sim}2.93cmol^+$(Sunchon) ; $7.44(Masan){\sim}18.58cmol^+$(Changweon) ; $2.82(Changnyong){\sim}6.24cmol^+/kg$(Changweon), respectively. There were high levels of phosphorus and potassium in the leaves of plastic film house plants of pepper, cucumber, pimento, chrysanthemum, and carnation. And calcium and magnesium were more than two fold high in fruiting vegetables than flowering plants showing antagonistic utilization against potassium.

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Increment of fructan biosynthesis in rice by transformation of 1-sst and 1-fft genes isolated from jerusalem artichoke (Helianthus tuberosus L.) (돼지감자 유래 1-sst와 1-fft 유전자의 형질전환 발현에 의한 벼의 fructan 생합성 증진)

  • Kang, Kwon-Kyoo;Song, Beom-Heon;Lee, Gyong-A;Lee, Hye-Jung;Park, Jin-Ha;Jung, Yu-Jin;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.37 no.1
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    • pp.102-109
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    • 2010
  • Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in $T_2$ generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.

Acquisition of Thermotolerance in the Transgenic Plants with BcHSP17.6 cDNA (BcHSP17.6 cDNA의 도입에 의한 형질전환된 식물의 내열성 획득)

  • Ki Yong Kim;Min Sup Chung;Jin Ki Jo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.17 no.4
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    • pp.379-386
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    • 1997
  • Recombinant plasmid, pBKH4, containing NPT II and P35S-BcHSP17.6 was constructed by ligation of Bum H I -digested pBKSl-l and BcHSP 17.6 (thermotolerance gene) 6om pBLH4. The tobacco leaf disc was cocultivated with transformed Agmbacterium tumefaciens bearing pBKH4 for 24 hours and transformed shoots were selected on MS-n/B medium containing $100\;{\mu\textrm{g}}/ml$ of kanamycin. Heat-killing temperature of Nicotima tabacum was $50^{\circ}$ for >15min, and transformed tobacco plants with BcHSP17.6 cDNA exhibited thermotolerance at the heat-killing temperature. The transgenic plants were analyzed by Southern blot hybridization with the probe of ${\alpha}^{_32}P$ labelled BcHSP17.6 cDNA. Transcription and expression level of BcHSP17.6 cDNA were also continued by Northern blot analysis and Ouchterlony double immunodiffusion assay. In this study, we suggest that the BcHSP17.6 cDNA introduced to tobacco plant is related to thenuoto-lerance and 17.6-kD LMW HSP acts as a protector from heat damage in plants.

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Generation of Transgenic Plant (Nicotiana tabacum var. Petit Havana SR1) harboring Bacillus thuringiensis Insecticidal Crystal Protein Gene, cry II A (Bacillus thuringiensis 살충성 결정단백질 유전자(cry II A)의 형질전환 식물 제작)

  • 이정민;류종석;권무식
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.5
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    • pp.305-311
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    • 1997
  • Bacillus thuringiensis, a gram-positive soil bacterium, is characterized by its ability to produce crystalline inclusions during sporulation. The crystal proteins exhibit a highly specific insecticidal activity. An insecticidal crystal protein (ICP), Cry II A, is specifically toxic to both lepidopteran and dipteran insects. In this study, tobacco plants transformed by the cry II A gene have been generated. The Cry II A crystal protein was purified from E. coli JM103 harboring cry II A gene by differential solubility. The activated Cry II A was prepared by tryptic digestion. The purified protoxin (70 kDa) and the activated toxin (50 kDa) were analyzed by SDS-PAGE. To generate the transgenic tobacco having cry II A gene, the cry II A gene was subcloned to a plant expression vector, pSRL2, having two CaMV 35S promoters. The recombinant plasmid was transformed into tobacco (N. tabacum var. Petit Havana SR1) by Agrobacterium-mediated leaf disc transformation. Through the regeneration, six putative transgenic tobacco plants were obtained and three transformants were confirmed by Southern blot analysis. It has been found that one plant had single copy of cry II A gene, another had two copies of the gene, and the third had a truncated gene. After the immunochemical confirmation of cry II A expression in plants, the transgenic tobacco plants will be used to study the genetics of future generation with the insecticidal crystal protein gene cry II A.

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Transplantation of Koelreuteria paniculata by Sea Current (모감주나무의 해류에 의한 전파)

  • 이영노
    • Journal of Plant Biology
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    • v.1 no.1
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    • pp.11-20
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    • 1958
  • I. Assumption: Mo-kam-ju, Koelreuteria paniculata is a woody plant mainly cistributed over Northern China. Its sporadic growth along the beaches of Korea and Japan is assumed to have been sowed by the seeds transported adrift on the current across the ocean. II. Use and Growth of the Plant: In China, this plant has been cultivated from early times mainly at temples, it seeds being used as rosaries, its flowers for yellow dyes and medicine for the eyes, and its leaves for black dyes. In Korea and Japan, these plants have been cultivated at temples and used as the material for rosaries. No natural growth of these plants was reported until 1919. III. Discoveries: In this domestic area, Dr. Chung Tae Hyun discovered the plant on the beach between Cho-Do and Chang-san-kot, Hwang-Hae Prov. in 1920. The reporter discovered them on the beach at Buk-Ni, Duk-jok-Do in 1948, at An-Hung in 1956 and on the beach at An-min-Do in 1957. In the Japan area, it was discovered for the first time twenty years ago, mainly along the coast line of the Japan Sea and some along the eastern coast line, at Subo, Yamaguchi Prefecture facing the Pacific Ocean. IV. Study and Experiment: A. Seed The seed coat is thick, non-permeable and floatable. A number of seeds were immersed in artificial sea water and fresh water separately. The seeds remained there for a hundred and forty-five (145) days from April 6, 1957 to August 29, 57. Thirty one seeds out of the fifty immersed in salt water and twenty seeds out of the fifty immersed in fresh water remained on the surface of the water, proving them to be non-permeable to both sea and fresh water. Of course, these had retained their germinating capabilities. Five (5) seeds out of twenty from the fresh water and six (6) out of thirty-one from the sea water were successfully germinated after a hole had been drilled in the seed coat and they had been planted. Thus their floating capability, non-permeability and germinating capability after a possible 145 day trip on the flowing currents has been proved satisfactory according to the assumption made above. B. Current As shown in the Data 2 and 3, the sea current initiated in the Pohai Sea flows westward down along the coast line of Korea reaching the southern part during the autumn and winter seasons. This fact also is in favor of the reporter's assumption. V. Discussion and Conclusion: The reporter concludes that, as discussed above, Koelreuteria paniculata which originates in Northern China is transported adrift on the flowing sea current to our western coast line, and also the coast lines of Shantung and Kuangtung of China and it is germinated on the sandy beaches forming new plants. Thus, the seeds drifted down on the southern beach of Korea and have been transported to the Japanese coast, adrift on the Tae-Ma current. Upon fruition, the seeds of the plants which settle on the coasts of western Korea and Japan will migrate to new places. It can be, however, assumed that while the thickness and nonpermeability of the coat enables the long travel in the water, this also can compose a difficulty in germination, consequently in developing a new distribution of this species.

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Development of Bialaphos-Resistant Transgenic Rice Using Agrobacterium tumefaciens (Agrobacterium tumefaciens를 이용한 bialaphos 저항성 형질전환 벼의 개발)

  • 이효연;이춘환;김호일;한원동;최지은;김진호;임용표
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.4
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    • pp.283-288
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    • 1998
  • The bialaphos is a potent inhibitor of glutamine synthease in higher plants and is used as a non-selective herbicide. We have used the bialaphos resistant gene(Bar) encoding for an acetyltransferase isolated from Streptomyces hygroscopicus SF1293. Callus derived from mature seeds of rice(Oryza sativa L. cv. Dong Jin) were co-cultivated with Agrobacterium tumefaciens EHA101 carring a plasmid pGPTV-HB containing genes for hygromycin resistance (HygR) and Bar. Transgenic plants showing in vitro resistance to 50 mg/L hygromycin and 10 mg/L bialaphos were obtained by using a two-step selection/regeneration procedure. Transformation efficiency of rice was about 30% which was as high as reported in other dicotyledons. Progenies ($\textrm{T}_{1}$ generation) derived from primary transformant of 17 lines were segregated with a 3 resistant : 1 sensitive ratio in medium containing hygromycin and bialaphos. Stable integration of Bar gene into chromosomal DNA was proven by Southern blot analysis of genomic DNA isolated from $\textrm{T}_{2}$ progenies. Transgenic plants ($\textrm{T}_{3}$) grown in the field were resistant to bialaphos (Basta) at a dosage lethal to wild type plants.

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The List of Vascular Plants at Junam Wetland in Changwon City (창원시 주남 습지의 관속식물상)

  • Lee, Ki-Suk;Cho, Min-Gi;Moon, Hyun-Shik;Jeon, Kwon-Seok
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.15 no.2
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    • pp.67-75
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    • 2013
  • The purpose of this study was to collect the basic data on establishing the conservation plan for the domestic wetland through flora and life form analysis at Junam wetland. The vascular plants were recorded as 222 taxa; 58 families, 167 genera, 200 species, 3 subspecies, 1 forma and 18 varieties. Among 8 life forms, Therophytes (Th), Hemicryptophytes (H) and Hydrophytes (HH) showed high percentage, and these species comprised 71.1% of the whole flora at Junam wetland. One taxa (Euryale ferox) rare plants designated by Korea Forest Service were observed. The naturalized plants were 38 taxa including Fallopia dumetora, Oenothera odorata, Ambrosia artemisiifolia var. elatior and so forth, and naturalized ratio was 17.1%. The naturalized made up about 45 percent of the species composition. The specific plants by floristic region were 13 taxa including Euryale ferox., Hydrocharis dubia, Prunus davidiana and so forth.

Development of Transgenic Tall Fescue Plants from Mature Seed-derived Callus via Agrobacterium-mediated Transformation

  • Lee, Sang-Hoon;Lee, Dong-Gi;Woo, Hyun-Sook;Lee, Byung-Hyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1390-1394
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    • 2004
  • We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. Transformation efficiency was influenced by the A. tumefaciens strain, addition of the phenolic compound acetosyringone and duration of vacuum treatment. Of the three A. tumefaciens strains tested, EHA101/pIG121Hm was found to be most effective followed by GV3101/pIG121Hm and LBA4404/pIG121Hm for transient GUS expression after 3 days co-cultivation. Inclusion of 100 $\mu$M acetosyringone in both the inoculation and co-cultivation media lead to an improvement in transient GUS expression observed in targeted calli. Vacuum treatment during infection of calli with A. tumefaciens strains increased transformation efficiency. The highest stable transformation efficiency of transgenic plants was obtained when mature seed-derived calli infected with A. tumefaciens EHA101/pIG121Hm in the presence of 100 $\mu$M acetosyringone and vacuum treatment for 30 min. Southern blot analysis indicated integration of the transgene into the genome of tall fescue. The transformation system developed in this study would be useful for Agrobacterium-mediated genetic transformation of tall fescue plants with genes of agronomic importance.

Improvement of Transformation Efficiencies using Agrobacterium-Mediated Transformation of Korean Rice

  • Cho, Joon-Hyeong;Lee, Jang-Yong;Kim, Yong-Wook;Lee, Myoung-Hoon;Park, Seong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.61-68
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    • 2004
  • A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.