• Journal of Ecology and Environment
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• 제43권1호
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• pp.9-13
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• 2019

We investigated the pollinators of Centipede's foot orchid (Cleisostoma scolopendrifolium) at five locations in southern Korea. Only one species of megachilid bee (Megachile yasumatsui) was observed at three of the sites (Mokpo, Haenam, and Wando). We assumed that the megachilid bee worked as a sole pollinator of C. scolopendrifolium based on the visiting behavior of the bees the attached pollinia. Fruits were observed at the three locations that bees visited: 74% fruit set at Mokpo, 59% at Wando, and 31% at Haenam. By contrast, at two of the locations where megachilid bees did not visit the plants (Naju and Jindo), there were no fruits set. The differences in fruit set rates at the three orchid populations where bees were observed appear to be related to bee abundance and flower visitation rate rather than to differences in flower abundance. The pollination interaction between C. scolopendrifolium and megachilid bees appears to involve sexual deception since only male bees were attracted to the flowers. The underlying mechanism involved in this interaction needs investigation.

• 한국자원식물학회지
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• 제29권2호
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• pp.225-234
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• 2016

조선시대 15세기부터 19세기에 편찬된 5개의 문헌, 세종실록지리지, 신증동국여지승람, 탐라지, 남환박물, 제주계록에 실린 문헌을 해제 분석하고, 제주도에 서식하는 자원이 되는 관속식물을 조사 분석해 고전명과 현대명을 분석 정리하여 관련된 전통지식을 파악하였다. 그 결과 65과 112속 118종 13변종으로 총131분류군이 확인되어 현 제주도 자생식물의 7.3%에 해당하는 식물들이 조사되었다. 이 중 21.4%가 남방계식물이었다. 5개의 문헌 중 식물종류가 가장 많이 기록된 문헌은 남환박물로, 89분류군이 기록되었다. 고전식물명을 현대식물명의 일치여부를 분석 조사하였고, 아울러 그 중 문제가 되었던 식물들에 관해 논의하였다.

• Journal of Plant Biotechnology
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• 제38권4호
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• pp.272-277
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• 2011

Plants expressing Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant EPSPS. In order to develop herbicide-resistant rice, we prepared transgenic rice plants with CP4 EPSPS gene under the control of CaMV 35S promoter for over-expression. A recombinant plasmid was transformed into rice via Agrobacterium-mediated transformation. A large number of transgenic rice plants were obtained with glyphosate and most of the transformants showed fertile. The integration and expression of CP4 EPSPS gene from regenerated plants was analyzed by Southern and northern blot analysis. The transgenic rice plants had CP4 EPSPS enzyme activity levels more than 15-fold higher than the wild-type plants. EPSPS enzyme activity of transgenic rice plants was also identified by strip-test method. Field trial of transgenic rice plants further confirmed that they can be selectively survived at 100% by spay of glyphosate (Roundup$^{(R)}$) at a regular dose used for conventional rice weed control.

• 한국환경복원기술학회지
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• 제13권2호
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• pp.24-41
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• 2010

Distributional characteristics of vascular plants and plants selection for revegetation in bare ground of lakeside was investigated in nine artificial lakes. The vascular plants were composed of 64 families, 194 genera, 268 species, 3 subspecies, 30 varieties, 3 forma, totaling 304 taxa. Dominant species of nine investigated areas were represented as Ambrosia trifida, Calystegia hederacea, Matricaria matricariodes, Xanthium italicum. Dominant species of three parts based on the location of artificial lakes were as follows:Ambrosia trifida, Bidens tripartita, Calystegia hederacea, Equisetum arvense, Setaria viridis, Spergula arvensis, Torilis japonica in northern part (Lake Paro and Soyang); Calystegia hederacea, Matricaria matricariodes, Rumex crispus, Xanthium italicum in middle part (Lake Chungju, Daecheong, Andong and Imha); and Ambrosia artemisiifolia, Bidens frondosa, Chenopodium glaucum, Miscanthus sinensis var. purpurascens, Persicaria pubescens, Setaria viridis in southern part(Lake Juam, Hapcheon and Jinyang). Among the 304 taxa, Korean endemic, endangered and rare plants were not investigated. The naturalized plants were 46 taxa, and life form was Th-$R_5-D_4$-e type. Above the results, Calystegia hederacea, Equisetum arvense, Xanthium italicum, Matricaria matricariodes, and Spergula arvensis were considered with proper species for revegetation in bare ground of lakeside in artificial lakes.

• 식물분류학회지
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• 제38권3호
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• pp.315-332
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• 2008

서해 중부지역에서의 상록활엽식물의 분포를 밝히기 위해 2006년 5월부터 2008년 3월까지 인천광역시에 속하는 136개 도서를 조사하였다. 이번 조사에서 28개 유인도와 49개 무인도 등 총 77개 도서에서 12종의 상록활엽식물이 조사되었다. 이 중 가장 흔한 종은 사철나무, 보리밥나무, 동백나무, 도깨비쇠고비 등이었다. 이들 식물은 또한 조사지역중 최북단에 위치한, $N 37^{\circ}60^{\prime}$ 부근 백령도에서도 자란다. 한편 송악, 붉가시나무, 참식나무, 마삭줄 등 4종은 $N 37^{\circ}10^{\prime}$ 부근 덕적면까지만 발견된다. 그러나 이들 식물은 육지에서는 한반도 남쪽이나 서남해안 지역까지 자란다. 상록활엽식물이 이런 고위도 도서지역까지 분포하는 것은 서해 난류의 영향으로 보인다. 현지조사와 문헌을 기초로 각 도서 별 상록활엽식물의 분포를 기재하였다.

• 한국자원식물학회지
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• 제14권3호
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• pp.259-276
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• 2001

서운산(574m)은 지리적으로 북위 36$^{\circ}$54'~ 36$^{\circ}$50', 동경 127$^{\circ}$ 16'~127$^{\circ}$19'사이에 위치하며 경기도와 충청북도에 걸쳐 있다. 본 연구는 2000년 3월~10월까지 9차례에 걸쳐 실시한 현지조사의 결과를 종합하여 소산 식물의 목록을 작성하였다. 조사지의 소산 식물은 90과 268속 383종 49변종 5품종 2교잡종 등 총 439종류로 확인되었다. 산림청 지정의 희귀 및 멸종위기 식물은 천마, 뻐국나리, 삼백초, 태백제비꽃 등이 조사되었으며, 특산식물은 총 12종으로 전체 소산식물의 2.7%를 차지하였다. 귀화식물은 총 24종류로 전체 소산식물의 5.5%에 해당되었다. 서운산에서 조사된 자원식물의 용도별 구분은 식용자원식물이 239종류(54.4%), 약용 자원식물이 270종류(61.5%), 관상용 자원식물이 217종류(49.4%), 목재용 자원식물이 46종류(10.5%), 사료용 자원식물이 56종류(12.8%)로 구분되었다.

• Journal of Plant Biotechnology
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• 제2권1호
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• pp.35-41
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• 2000

Transgenic cabbage (Brassica oleracea ssp. capitata) plants resistant to the commercial herbicide Bast $a^{R}$ were obtained by Agrobacterium tumefaciens - mediated transformation. Hypocotyl segments of in vitro grown plants were infected with Agrobacterium tumefaciens LBA 4404 harboring plasmid pMOG6-Bar which contains hpt and bar genes. Explants were cultured on callus induction medium (MS basal medium + 1 mg/L NAA + 2 mg/L BA + 2 mg/L AgN $O_3$+ 100 mg/L carbenicillin + 250 mg/L cefotaxime) supplemented with 15 mg/L hygromycin. Hygromycin resistant calluses were transferred to shoot regeneration medium (MS basal medium + 0.1 mg/L NAA + 2 mg/L BA + 3% sucrose + 2 mg/L AgN $O_3$+ 15 mg/L hygromycin + 250 mg/L cefotaxime + 100 mg/L carbenicillin). In order to induce roots, elongated shoots were placed on the MS medium without plant growth regulators and hygromycin. Southern blot analysis of several putative transgenic plants indicated that one to five intact copies of Apt and bar genes were incorporated into the genome. Expression of bar gene was confirmed by Northern blot analysis and by herbicide resistant phenotype. Seed progeny from self-pollinated transformants expressed the herbicide resistance and showed Mendelian segregation of the introduced gene.e.

• 한국초지조사료학회지
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• 제20권2호
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• pp.97-102
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• 2000

To increase thennotolerance of forage crops, transgenic rice plants as a model for transformation of monocots were generated. A cDNA encoding the chloroplast-localized small heat shock protein (small HSP) of rice, Oshsp21, was introduced into rice plants via Agrobacterium-mediated gene transfer system. Calli induced from scutella were co-cultivated with a A. tumefaciens strain EHAlOl canying a plasmid, pIGhsp21. A large number of transgenic plants were regenerated on a medium containing hygromycin. Integration of Oshsp2l gene was confirmed by PCR and Southern blot analyses with genomic DNA. Northern blot and immunoblot analyses revealed that the Oshsp21 gene was constitutively expressed and accumulated as mature protein in transgenic plants. Effects of constitutive expression of the OshspZl on thermotolerance were first probed with the chlorophyll fluorescence. Results indicate that inactivation of electron transport reactions in photosystem I1 (PSII), were mitigated by constitutive expression of the Oshsp21. These results suggest that the chloroplast small HSP plays an important role in protecting photosynthetic machinery during heat stress. (Key words : Thermotolerance, Rice, Transgenic, cDNA)

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.233-236
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• 2006

Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.

• 한국식물병리학회지
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• 제14권5호
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• pp.536-542
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• 1998

Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (Cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophyica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorephic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observe by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different form different isolates due to the genetic variations.