• 한국작물학회지
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• 제60권2호
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• pp.248-252
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• 2015

This study was conducted to compare the growth, inorganic components, and proximate components of Codonopsis lanceolata grown in 10 regions of Korea for selecting superior species and breeding by crossing. Among the all tested lines, the shortest plant height (217.12 cm) was observed from the Ulleungdo region line (No. 4) while the longest (273.9 cm) was observed from Hwasun region line (No. 9). In addition, the lines of central and northern region (No. 1~No. 7) tend to have shorter plant height than those of southern region (No. 8~No. 9) except Jejudo region line (No. 10). Flowering tends to be late towards southern region, and lines in central and northern regions were started flowering about 2 weeks earlier than those in southern regions. However, the heaviest root weight was 13.1 g, found in only Jejudo line (No. 10) whereas there was no significant difference found in the other regions which have a range of 8.3~11.0 g. The inorganic components were varied in each line, however, proportion of macroelements, such as K, Ca, and P, was the largest for every line. Especially for Heongseong region line (No. 2), had larger proportion of macroelements than the others. There was a difference of proximate compositions of Codonopsis lanceolata, except the moisture content, among all regions, however, it was generally shown that the content of crude protein (1.31~3.76%) and crude fiber (2.18~3.12%) was the highest.

• 식물조직배양학회지
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• 제27권6호
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• pp.441-445
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• 2000

저인산 조건하에서 인산 흡수 효율이 높은 벼 품종을 개발하고자 담배의 인산수송자 유전자를 벼에 형질전환하였다. 동진벼로부터 유도된 callus를 담배의 인산수송자 유전자가 도입된 A. tumefaciens LBA 4404와 공동배 양한 후, 선발배지에서 증식된 callus를 250 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA가 첨가된 MS배지에 옮겨 약 2주 후부터 소식물체를 얻었다. Carbenicillin 250 mg/L 첨가된 MS 기본배지에서 소식물체의 발근을 유도하여 재분화 식물체를 얻었다. 선발된 callus는 약 52%의 식물체 재력화율을 보였다. 선발된 재분화 식물체에 대한 PCR 분석을 수행하여 형질전환 벼 식물체에 담배의 인산수송자 유전자가 삽입되었음을 확인하였다. 형질전환체의 genonlic DNA로부터 PCR에 의해 증폭된 DNA에 대한 Southern blot분석 결과, 대조 식물체에서는 band가 검출되지 않았으나 형질전환 식물체에서는 인산수송자 유전자와 동일한 1.7kb의 band가 검출되어 외래유전자인 담배 인산수송자 유전자가 안전적으로 도입되었음이 확인되었다.

• Journal of Plant Biotechnology
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• 제30권4호
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• pp.335-339
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• 2003

pSK109를 포함하고 있는 Agrobacterium LBA4004을 통하여 국화 'Shuho-no-chikara'에 LEAFY 유전자을 도입하였다. 엽절편체의 생존율은 kanamycin 10mg/L가 첨가된 배지에서 생존율 약 22%, 절편체의 재분화율이 10%정도였다. 그러나 kanamycin 20mg/L가 첨가된 배지에서는 엽절편은 약 5%정도 생존하였으나 전혀 재분화가 나타나지 않았다. 따라서 kanamycin 10mg/L를 1차 선발배지로, 20mg/L를 2차 선발배지로 선발하였다. Agrobacterium LBA4404와 국화 엽절편체를 3일간 공동배양 하는 것이 형질전환에 효과적이었다. LEAFY유전자가 삽입된 pSK109 vector를 포함한 Agrobacterium LBA4404의 형질전환 효율은 2차 선발까지 약 2.8% 였으며, Southern blot한 결과 0.13%만이 형질 전환체로 확인되었다. 형질 전환체 한계통은 포장에서 약 1주일 정도 조기개화하였고 화형은 정상이었다.

• 한국동물위생학회지
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• 제36권3호
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• pp.151-155
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• 2013

Since a large number of Akabane and bovine ephemeral fever (BEF) infection occurred in the southern part of Korea in 2010, recent information about seroprevalence of Akabane virus (AKAV) and bovine ephemeral fever virus (BEFV) has been required for preventing both diseases. In this study, serological assay against AKAV and BEFV using virus neutralization assay was conducted using 1,743 bovine sera collected from Namwon, Miryang, Yeongju and Uljin which located in Southern part of Korea from March to May in 2012. The overall seropositive rates for AKAV and BEFV were found to be 49.8% and 1.2%, respectively. The regional distribution of seroprevalence for AKAV ranged from 18.1% to 63.7%. Seroprevalences of AKAV were 63.7% in Miryang, 62.3% in Uljin, 50.7% in Namwon, and 18.1% in Yeongju. The seropositive rates for AKAV in southern part of Korea were higher than the annual average at the national level. On the other hand, seropositive rates of BEFV in four regions were from 0.3 to 3.1%. In detail, regional seroprevalences were 3.1% in Miryang, 2.0% in Uljin, and 1.7% in Yeongju, and 0.3% in Namwon. Even only one year after massive outbreaks, overall seropositive rates were very low, similar to the annual average at the nation level. This result indicates that many number of cattle infected with BEFV may be replaced by new born calf or cattle in farm may not be immunized with vaccines. To prevent another epidemic, a national wide warning should be issued and more aggressive control measure must be implied. Recent global warming phenomenon could lead to more vigorous activity of haematophagous vectors and it is possible that arboviral diseases such as AKAV and BEFV are increased. Therefore, continuous sero-monitoring and extensive vaccination combined with control of haematophagous vectors are important to effectively prevent and control diseases caused by AKAV and BEFV.

• 한국자원식물학회지
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• 제27권4호
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• pp.333-336
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• 2014

지중해 연안의 남유럽과 서남아시아 원산으로 전 세계에 널리 퍼져 분포하는 귀화식물인 Trifolium resupinatum L.(콩과)을 전라남도 진도의 의신천 제방에서 발견하였다. 이 귀화식물은 같은 속의 식물들에 비해 뒤집힌 꽃을 갖는 특징으로 쉽게 구분되며, 이 특징에 따라 거꿀꽃토끼풀이라는 국명을 새로 지었다.

• 식물분류학회지
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• 제52권3호
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• pp.196-200
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• 2022

Verbena bracteata Cav. ex Lag. & Rodr. (Verbenaceae), native to North America and an alien plant in China and Japan, was newly found in central and southern areas of Korea. This species can be distinguished from other Verbena taxa in Korea by having larger floral bracts and stems that are prostrate or procumbent to decumbent-ascending. Verbena bracteata grows in dry and sunny grasslands or along roadsides. A precise description, illustration, photographs, and a key to related taxa are provided.

• The Korean Journal of Ecology
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• 제17권3호
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• pp.391-413
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• 1994

The northernmost type of the monogolian oak forests in the Russian Far East was studied in terma of syntaxonomy and synchorology. Hand-sorting method, computer program TWIN-SPAN, CANOCO and SYN-TAX III were engaged for the classification and data analysis. Correlation between plant communitie and environmental factors was analyzed by DCA (Detrended Correspondence Analysis) using NeCD (Net Contribution Degree) of species. Three plant communities were recognized: the Abieti holophyllae-Quercetum mongolicae, the Lespedezo-quercetum mongolicas and the Rosa ussuriensis-Quercus mongolica communit. They were included into the alliance Jeffersonio-Quercion monogolicae which is representative to the cool-Temperate forests mixed by conierous and broadleaved trees in southern Sikhote Alin. Human impact was signified as the most important factor to interpret the disjunction of plant communities of which DCA ordination yielded sharp contrasting objects (i.e. stands and species). In the amhi-Tonghae region, alaogous vegetation types such as the Jeffersonio-Quercion monogolicae of Russian Far East, the Quercion grosseserratae of Japanese Hokkaido and the Pino koraiensis-Quercion monogolicae of Koream peninusula were reviewed in terms of similarity of species composition and stratum structure. It is signified that the Jeffersonio-Quercion monogolicae was one of the continental types more similar to Korean alliance that to Japanese one.

• Journal of Photoscience
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• 제12권2호
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• pp.75-82
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• 2005

Rumex acetosa L. is a dioecious flowering plant with well developed sex chromosome system: 2n = 12 + XX in the female plants and 2n = 12 + XY1Y2 in the male plants. To isolate sex-linked DNA, we carried out chromosome micromanipulation, followed by DOP-PCR, AFLP of the PCR products, reverse Southern hybridization and sequence analysis. From 500 AFLP specific clones, 13 X-chromosome and 5 Y-chromosome specific clones were obtained. Except one clone RADAX-239 ($\underline{R}umex\;\underline{a}-\underline{D}OP-PCR-\underline{A}FLP-\underline{Y}-chromosome\;specific$), all clones appear to be R. acetosa plant-specific sequences and non-coding sequences. Southern blot analysis using these clones could not discriminate genomic DNAs either from male or female plants. Results of this study imply that both autosome-origin and degeneration of sex chromosomes are prevalent in plant systems.

• 한국약용작물학회지
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• 제15권5호
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• pp.357-361
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• 2007

This study was conducted to evaluate the vegetation structure of the Peucedanum japonicum community by the phytosociological method of floristic composition table and cluster analysis on the southern coast of Korea. The vegetation of the Peucedanum japonicum community was classified mainly into 2 communities such as the Miscanthus sinensis community and the Lysimachia mauritiana-Rosa wichuraiana community. The Carex boottiana and Sedum oryzifolium community were classified as the lower rank of Miscanthus sinensis community. On level 1 of the cluster analysis of plant species, they were classified into Lysimachia mauritiana and Rosa wichuraiana group, also Miscanthus sinensis, Carex boottiana and Sedum oryzifolium in Peucedanum japonicum community, which is similar to the community classification shown in the synoptic table. On level 1 of the cluster analysis of relev, inland coast with Jejudo was Lysimachia mauritiana and Rosa wichuraiana of group such as level 1 of the cluster analysis of plant species, and island coast with Geomundo was Miscanthus sinensis Carex boottiana and Sedum oryzifolium of group such as cluster analysis of plant species.

• 식물조직배양학회지
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• 제28권1호
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• pp.47-52
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• 2001

구기자나무의 효과적인 재분화조건을 바탕으로 염류내성유 전자인 Bet A와 Bet B유전자의 도입을 시도하였다. 구기자나무의 절편체를 재료로 kinetin 1 mg/L, IBA 0.05 mg/L가 첨가된 MS배지에 2일간 전배양한 후 Agrobacterium과 공조배양 및 선발배지에서의 배양으로 kanamycin에 내성을 갖는 잠정적인 형질전환체를 유도하였다. 형질전환체는 PCR 기법 및 Southern blot분석으로 Bet A와 Bet B 유전자 전이를 확인하였고, 도입된 유전자의 발현은 RT-PCR 방법을 사용하여 확인하였다.