• Journal of Ecology and Environment
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• 제42권1호
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• pp.30-42
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• 2018

Background: The southern and eastern parts of the Gobi Desert area are a unique dry ecosystem with a diverse regional desert, semi-desert, and mountain dry steppe flora. This area habitat is located at the overlap of different floristic regions; on its northeast side, Central Asian desert flora is dominating, and on the eastern side, East Asian flora is observed. The comprehensive survey was carried out to find the floral diversity of the medicinal plants on the region. Methods: All recorded species in this study were based on the collected voucher specimens between June and August in the year 2017. Results: We recorded 23 families, 57 genera, and 78 species of vascular plants. The families Asteraceae (15 species), Fabaceae (10 species), and Amaranthaceae (10 species) were represented most in the study area, while Caragana (5 species), Salsola (4 species), and Arnebia (3 species) were the most common genera found. Conclusion: Conservation status for remarkable species was also reviewed based on the literature. Around the study area, 24 species as "sub-endemic," 10 species as "very rare," 4 species as "rare," 1 species as "alien," 13 species as "relict," 10 species as "Red Book," 2 species as "endangered (EN)," 3 species as "vulnerable (VU)," 3 species as "near threatened (NT)," and 2 species as "least concern (LC)" plants are growing.

• 아시안잔디학회지
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• 제25권2호
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• pp.184-189
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• 2011

금잔디(Zoysia matrella (L.) Merr.)의 포복경 조직으로부터 식물체 재분화를 위한 최적 조직배양조건을 확립하기 위하여 온실에서 재배한 식물체의 포복경 절편체로부터 식물체 재분화에 미치는 몇 가지 요인을 조사하였다. 포복경 조직으로부터 식물체 재분화에 있어서 기본배지로는 MS배지가 N6배지나 SH배지에 비해 높은 효율을 보였으며, 배지에 첨가되는 식물생장조절물질로는 0.5 mg/L 2,4-D와 1 mg/L of kinetin을 첨가했을 때 33.3%로 가장 높은 재분화율을 보였다. 또한 배지에 첨가되는 탄소원 종류로는 sucrose를 3% (v/v) 농도로 첨가해 주었을 때 재분화 효율이 향상되었으며, 재분화배지에 첨가되는 항산화물질로는 5 mg/L $AgNO_3$와 10 mg/L cysteine을 동시에 첨가해 주었을 때 40%의 가장 높은 식물체 재분화 효율을 나타내어 재분화효율이 향상되었다. 본 연구를 통하여 확립한 금잔디의 포복경 재분화 시스템은 캘러스 배양기간 없이 단기간 내에 재분화 식물체를 높은 효율로 얻을 수 있음으로써 유전자 형질전환을 통한 잔디의 분자육종에 있어서 유용하게 사용되어질 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제31권4호
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• pp.319-323
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• 2004

Euphorbia lathyris의 유관세포로부터 분비되는 수용성 latex단백질을 10% SDS-polyacrylamide 전기영동으로 분리하여 특징적으로 잘 나타나고 있는 ELp65, ELp55, ELp43, ELp32 그리고 ELp23 등의 주요단백질을 확인하였다. 이들 latex 주요단백질들 중에서 ELp65는 ammonium sulfate 침전, gel permeation chromatography 그리고 ion exchange chromatography 등의 방법으로 순수 분리하였는데 ELp65의 N-terminal amino acid 서열분석에 의하면 이는 토마토의 p69a subtilisin-like pretense의 mature peptide의 앞부분과 강한 유사성을 지니고 있었으며 식물방어와 관련된 기능이 제시되었다. PCR증폭에 의하여 클로닝된 토마토의 p69a DNA를 probe로 이용하여 Southern blot hybridization을 수행한 결과 E. lathyris genome은 토마토의 subtilisin-like proteases와 유사한 정보를 지닐 수도 있는 3-5 유전자들로 구성된 gene family가 분석되었다.

• Plant Resources
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• 제5권2호
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• pp.104-108
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• 2002

In order to obtain basic information for selecting early maturing rice varieties which is suitable for early cropping before Alisma plantago in the southern part of Korea. Six rice varieties were grown from May to August in 2002 at Youngjeon Experiment Field, Sunchon and yield components and yield of plants were investingated. Early maturing rice cv. Grubyeo showed higher rough rice yield than any other varieties used in the experiment. It showed high yield components, such as culm length, panicle length, number of panicles per plant, number of spikelets per panicle and ratio of ripened grains. Therefore, it was concluded that Grubyeo was the most suitable variety with high yield for the cultivation before growing of Alisma plantago at the southern part of Korea. The heritability of culm length number of spikelets per panicle and rough rice yield were high and that of panicle length number of panicle per plant, ratio of ripened grain and 1,000 grain wt. of milled rice were low. According to the result of path coefficient analysis, characters highly correlated with rough rice yield showed large direct effects on rough rice yield.

• The Plant Pathology Journal
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• 제33권3호
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• pp.238-248
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• 2017

Pokkah Boeng is a serious disease of sugarcane, which can lead to devastating yield losses in crop-producing regions, including southern China. However, there is still uncertainty about the causal agent of the disease. Our aim was to isolate and characterize the pathogen through morphological, physiological, and molecular analyses. We isolated sugarcane-colonizing fungi in Fujian, China. Isolated fungi were first assessed for their cell wall degrading enzyme capabilities, and five isolates were identified for further analysis. Internal transcribed spacer sequencing revealed that these five strains are Fusarium, Alternaria, Phoma, Phomopsis, and Epicoccum. The Fusarium isolate was further identified as F. verticillioides after Calmodulin and EF-$1{\alpha}$ gene sequencing and microscopic morphology study. Pathogenicity assay confirmed that F. verticillioides was directly responsible for disease on sugarcane. Co-inoculation of F. verticillioides with other isolated fungi did not lead to a significant difference in disease severity, refuting the idea that other cellulolytic fungi can increase disease severity as an endophyte. This is the first report characterizing pathogenic F. verticillioides on sugarcane in southern China.

• 한국산림과학회지
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• 제113권1호
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• pp.66-72
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• 2024

Determining the nutrient stoichiometry in plant organs is critical for understanding nutrient uptake and cycling in forest ecosystems. This study evaluated nutrient concentrations and stoichiometry in various plant organs (stem, bark, branches, and foliage) of species found in four warm-temperate forests in southern Korea. Cryptomeria japonica D. Don (CJ), Quercus serrata Thunb. (QS), evergreen broadleaved tree species (EB), and bamboo spp. (BB) were destructively sampled to measure nutrient (C, N, and P) concentrations in the plant organs. The mean C concentration in the stem was significantly higher in CJ than in QS, BB, or EB, whereas the C concentration in the foliage was the lowest in BB. The mean foliar N and P concentrations were higher in BB than in EB or CJ. The mean stem C:N and C:P ratios were highest in CJ but were lowest in the foliage of BB. Overall, stems of all species showed a strong positive correlation between C concentration and dry weight, but a negative correlation between N and dry weight. The N and P concentrations of foliage and bark were strongly correlated, whereas those of the stem and branches were poorly correlated. Positive correlations were detected between the C:N and C:P ratios in bark and foliage. These results indicate the existence of intraspecific differences in nutrient requirements in warm-temperate forest species and add to the understanding of nutrient uptake and storage patterns in the organs of species growing in warm-temperate forests.

• The Korean Journal of Ecology
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• 제25권5호
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• pp.341-348
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• 2002

본 연구는 환경부의 '전국 무인도서 자연환경조사 보고서(1999년∼2001년)'에서 발췌한 261개의 무인 도서를 대상으로 섬의 지리적 환경과 섬에 분포하는 관속식물의 종수 및 분포사이의 상관성을 구명하고자 수행하였다. 261개 도서의 평균 면적은 75,000㎡이며, 면적의 분포범위는 최소 1,l00㎡에서 최대 961,200㎡였다. 육지와의 최단거리 평균은 14.9km(150m∼51.4km)이었다. 261개 도서에서 관찰된 전체 종수는 30과 1,109 종으로, 각 도서당 관찰된 종수의 평균은 98.7종이었다. 자생식물은 1,003종(90.4%)이었으며, 귀화식물은 106종(9.6%)로 조사되었다. 그리고 조사된 전체 종수 중에서 국화과가 114종으로 가장 많았으며, 벼과 (90종), 콩과(54종), 장미과(53종) 순으로 나타났다. 261개 섬은 출현한 식물종의 구성에 근거하여 다차원척도분석을 실시한 결과 '남해그룹(130개 섬)'과 '서해그룹(131개 섬)'의 두 지역으로 뚜렷이 구분할 수 있었다. 서해그룹에 속하는 섬들은 남해그룹의 섬들에 비해 면적이 더 넓었으나(서해그룹 평균=93,000㎡, 남해그룹 평균=57,000㎡), 도서당 출현 종수는 적었다(서해그룹 평균=192, 남해그룹 평균=233). 또한 서해그룹보다는 남해그룹에만 한정적으로 분포하는 종들이 두 그룹을 구분하는데 더욱 큰 영향을 미치는 것으로 나타나서, 위도에 따른 식물 분포를 반영하고 있다. 출현하는 식물 종수에 가장 큰 영향을 미치는 인자는 섬의 면적이었으며, 육지와의 최단거리와 인간의 간섭 인자 순으로 나타났다. 식물종수에 미치는 환경인자들은 서해그룹과 남해그룹에서 각기 다르게 나타났으며, 남해그룹의 섬들이 서해그룹의 섬들에 비하여 육지와의 거리가 가깝고 단위면적당 출현 종수가 많았기 때문에 보다 다양한 환경인자들에 의해 민감하게 영향을 받는 것으로 생각된다. 따라서 이상의 결과들은 무인도서 생태계 보존을 위해 중요하게 활용될 것으로 기대되며, 앞으로 인간간섭과 귀화식물종이 무인도서 생태계에 미치는 영향에 대한 연구가 계속적으로 이루어질 것이다.

• 한국식물병리학회지
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• 제13권2호
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• pp.118-124
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• 1997

The two viruses of barley yellow mosaic(BaYMV) and barley mild mosaic virus (BaMMV) were detected by ELISA from barley plants with virus-like symptoms which were collected from 16 locations in southern Korea, during 1995 and 1996. Both viruses occurred in southern Korea. Barley plants at Chongdo and Koseong were infected with BaMMV, while those infected with BaYMV were at Kurye and Taegu. After sowing 50 barley cultivars at habitually infected fields in 10 locations, the susceptibility and resistance to BaYMV and BaMMV were screened with antiserum tests. The cultivars of Albori, Alchanbori, Daejinbori, Jokangbori, Milyangbori, Boeunkwamek, Naehanssalbori, Olssalbori, Weossalbori, Dusan 29 and Deogndohyangchonkwa showed positive reaction to BaYMV antiserum, while Saeolbori, Chalbori, Jinjukwa and Baegjinkwa showed positive reaction to BaMMV. Nonsankwa 1-6 and wheat cultivars of Chongkeymil, Dahongmil, Grumil, Urimil, Jochonhomil, Sinkeyhomil showed negative reactions to both viruses. The rest cultivars were infected both with BaYMV and BaMMV. Sap inoculations to barleyplants with the two viruses of BaYMV isolated in Haenam and BaMMV isolated at National Honam Agricultural Station, expressed lower infection rate than those grown in the virus-infected fields.

• The Plant Pathology Journal
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• 제28권4호
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• pp.428-432
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• 2012

We determined the complete genome sequence of a Vietnamese isolate of Southern rice black-streaked dwarf virus (SRBSDV). Whole genome comparisons and phylogenetic analysis showed that the genome of the Vietnamese isolate shared high nucleotide sequence identities of over 97.5% with those of the reported Chinese isolates, confirming a common origin of them. Moreover, the greatest divergence between different SRBSDV isolates was found in the segments S1, S3, S4 and S6, which differs from the sequence alignment results between SRBSDV and Rice black streaked dwarf virus (RBSDV), implying that SRBSDV evolved in a unique way independent of RBSDV. This is the first report of a complete nucleotide sequence of SRBSDV from Vietnam and our data provides new clues for further understanding of molecular variation and epidemiology of SRBSDV in Southeast Asia.

• The Plant Pathology Journal
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• 제32권2호
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• pp.102-111
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• 2016

Olive culture is very important in the Mediterranean Basin. A severe outbreak of Olive Quick Decline Syndrome (OQDS) caused by Xylella fastidiosa infection was first noticed in 2013 on olive trees in the southern part of Apulia region (Lecce province, southern Italy). Studies were carried out for detection and diversity evaluation of the Apulian strain of Xylella fastidiosa. The presence of the pathogen in olive samples was detected by PCR amplifying the 16S rDNA, gyrase B subunit (gyrB) and HL hypothetical protein genes and single nucleotide polymorphisms (SNPs) assessment was performed to genotype X. fastidiosa. Twelve SNPs were recorded over gyrB and six SNPs were found for HL gene. Less variations were detected on 16S rDNA gene. Only gyrB and HL provided sufficient information for dividing the Apulian X. fastidiosa olive strains into subspecies. Using HL nucleotide sequences was possible to separate X. fastidiosa into subspecies pauca and fastidiosa. Whereas, nucleotide variation present on gyrB gene allowed separation of X. fastidiosa subsp. pauca from the other subspecies multiplex and fastidiosa. The X. fastidiosa strain from Apulia region was included into the subspecies pauca based on three genes phylogenetic analyses.