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Effect of Plant Growth Regulators and Medium Supplements on Plant Regeneration from Stolon of Zoysia matrella Merr  

Kim, Kyung-Hee (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University)
Kim, Yong-Goo (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University)
Heo, Sung-Hyun (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University)
Bae, Eun-Ji (Southern Forest Research Center, Korea Forest Research Institute)
Lee, Kwang-Soo (Southern Forest Research Center, Korea Forest Research Institute)
Park, Nam-Chang (Southern Forest Research Center, Korea Forest Research Institute)
Lee, Byung-Hyun (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University)
Publication Information
Asian Journal of Turfgrass Science / v.25, no.2, 2011 , pp. 184-189 More about this Journal
Abstract
To optimize tissue culture conditions for genetic transformation of Zoysia matrella (L.) Merr., we investigated the effects of different plant growth regulators and medium supplements on plant regeneration using stolon explants excised from mature plant grown in a green house. Plant regeneration frequency was 33.3% when stolon tissues with a node were cultured on the regeneration medium supplemented with 0.5 mg/L 2,4-D and 1 mg/L of kinetin. Comparing the basal media tested, MS medium showed higher plant regeneration performance than N6 or SH medium. Addition of 5 mg/L $AgNO_3$ with 10 mg/L cysteine improved frequency of plant regeneration up to 40%. Among different carbon sources, 3% sucrose was found to show the best for regeneration frequency. This rapid and efficient plant regeneration system would be useful for using genetic transformation experiments of manilagrass without intervening callus-mediated regeneration.
Keywords
Plant regeneration; Stolon; Zoysia matrella;
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