• Journal of Veterinary Science
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• 제21권6호
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• pp.73.1-73.10
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• 2020

Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. Objectives: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. Methods: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. Results: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX₂CX₂₉CX₂C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. Conclusions: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1.

• 한국의류산업학회지
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• 제24권1호
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• pp.95-107
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• 2022

In reality, the distinction between the Korean traditional culture and the cultures of other countries is at a delicate boundary. Additionally in the wake of the recent socio-cultural confrontation between Korea and China, it has become necessary to establish the foundation and area of Korean traditional culture and to actively utilize the importance of improving awareness of Korean traditional culture. To reorganize the reckless use of the gold foil pattern shown in the rental hanbok, data on the museum's collection of gold leaf patterns were collected and analyzed. Based on the gold foil, Gilsang characters such as Phoenix pattern, Crane pattern, Bat pattern, Flower pattern, Fruit pattern and recovery advice were extracted through references. The traditional gold foil pattern was reconstructed and relocated to design the gold leaf patch. Based on the collection and analysis of the museum's relics, the Wonsam & Daedae, Dangeui, Sranchima, Sagyusam, Jeonbok, Bokgun, and Daenggi were produced. Therefore, we present the possibility of producing gold foil and modern methods for producing gold foil using laser cutting techniques that can express refinement and complexity well, and gold foil thermal transfer paper with retouchable effects. Additionally, we would like to reflect upon the practicality and the convenience to modern people by considering the complexity and hassle of the traditional gold foil production process, and the disadvantages of processes that require relatively longer time. It intends to help revitalize the market of Korean traditional clothing and fashion products.

• Structural Monitoring and Maintenance
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• 제9권1호
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• pp.29-42
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• 2022

In mountainous areas of China, concrete poles with connectors are widely employed in power transmission due to its convenience of manufacture and transportation. The bearing capacity of the poles must have degenerated over time, and most of the steel connectors have been corroded. Carbon fiber reinforced polymer (CFRP) offers a durable, light-weight alternative in strengthening those poles that have served for many years. In this paper, the bearing capacity and failure mechanism of CFRP sheet strengthened existing reinforced concrete poles with corrosion steel connectors were investigated. Four poles were selected to conduct flexural capacity test. Two poles were strengthened by single-layer longitudinal CFRP sheet, one pole was strengthened by double-layer longitudinal CFRP sheets and the last specimen was not strengthened. Results indicate that the failure is mainly bond failure between concrete and the external CFRP sheet, and the specimens fail in a brittle pattern. The cross-sectional strains of specimens approximately follow the plane section assumption in the early stage of loading, but the strain in the tensile zone no longer conforms to this assumption when the load approaches the failure load. Also, bearing capacity and stiffness of the strengthened specimens are much larger than those without CFRP sheet. The bearing capacity, initial stiffness and elastic-plastic stiffness of specimen strengthened by double-layer CFRP are larger than those strengthened by single-layer CFRP. Weighting the cost-effective effect, it is more economical and reasonable to strengthen with single-layer CFRP sheet. The results can provide a reference to the same type of poles for strengthening design.

• Journal of Veterinary Science
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• 제24권2호
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• pp.29.1-29.12
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• 2023

Background: Feline panleukopenia virus (FPV) is a widespread and highly infectious pathogen in cats with a high mortality rate. Although Yanji has a developed cat breeding industry, the variation of FPV locally is still unclear. Objectives: This study aimed to isolate and investigate the epidemiology of FPV in Yanji between 2021 and 2022. Methods: A strain of FPV was isolated from F81 cells. Cats suspected of FPV infection (n = 80) between 2021 and 2022 from Yanji were enrolled in this study. The capsid protein 2 (VP2) of FPV was amplified. It was cloned into the pMD-19T vector and transformed into a competent Escherichia coli strain. The positive colonies were analyzed via VP2 Sanger sequencing. A phylogenetic analysis based on a VP2 coding sequence was performed to identify the genetic relationships between the strains. Results: An FPV strain named YBYJ-1 was successfully isolated. The virus diameter was approximately 20-24 nm, 50% tissue culture infectious dose = 1 × 10^-4.94/mL, which caused cytopathic effect in F81 cells. The epidemiological survey from 2021 to 2022 showed that 27 of the 80 samples were FPV-positive. Additionally, three strains positive for CPV-2c were unexpectedly found. Phylogenetic analysis showed that most of the 27 FPV strains belonged to the same group, and no mutations were found in the critical amino acids. Conclusions: A local FPV strain named YBYJ-1 was successfully isolated. There was no critical mutation in FPV in Yanji, but some cases with CPV-2c infected cats were identified.

• 무역학회지
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• 제41권3호
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• pp.157-176
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• 2016

중국은 2014년 5월 1일부터 제3차 개정 중화인민공화국 상표법(《中华人民共和国商标法》)을 시행하고 있는바, 제3차 개정은 선사용 항변조항(제59조 제3항)을 신설하여 선사용인의 항변권을 법적으로 보호하기 시작하였다. 이에 대한 실무적 기준을 검토하기 위하여 본고는 중국최고인민법원이 공표한 금년도 10대 지식재산권판결 중 선사용 항변권이 인정된 판결을 중심으로 선사용 항변권이 인정되는 기준과 요건을 고찰한다.

• 식물병연구
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• 제29권2호
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• pp.101-107
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• 2023

배 검은별무늬병을 일으키는 원인 병원균은 Venturia nashicola는 국내를 포함한 일본, 중국, 대만에서 가장 문제가 되는 대표적인 병이다. 이 병을 관리하기 위해서는 주로 화학적 방제에 의존하는 방식으로 관리가 되고 있었다. 하지만 지속인 화학적방제는 환경오염뿐만 아니라 병원균의 저항성 출현을 야기하여 보다 지속 가능한 관리방안이 필요한 시점이다. 이에 V. nashicola의 생환 및 감염 특성을 이해하고 과거부터 이어져온 달력형 방제나 특정 계통의 살균제 연용에서보다 기상환경조건에 따라 능동적인 방제시기 설정이 필요한 상황이다. 이 논문에서는 배 검은별무늬병인 V. nashicola의 병환, 감염특성 그리고 방제체계에 대한 내용을 정리하고 보다 효과적인 방제관리체계에 대한 방안을 논의하고자 다양한 연구결과들을 리뷰하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.320-320
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• 2022

Coriander(Coriandrum sativum L.) belongs to the family Umbelliferae/Apiaceae. It is cultivated as a spice and medicinal herb around the world, including its leaves and seeds. Coriander leaves have soft and fragrant, so they can be used in cuisines such as China, Mexico, and, Southeast Asia. Coriander leaves contain a high amount of vitamin C, carotene, and multiple polyphenols. Coriander essential oils and extracts have various chemical components and are known to have antioxidant, antibacterial, and antifungal activities. This study was carried out for resource discovery, propagation, and DB construction of aromatic plants. In order to evaluate the genetic characteristics, 30 kinds of Coriander seeds were supplied from the Center for Genetic Resources. The evaluation of characteristics of the basal part leaf number, leaf shape, and plant height was investigated. Also, Essential oils extract from various parts of plants including the leaves, flowers, and steam isolated by simultaneous distillation extraction(SDE) apparatus. In the results, heights showed growing to 70 cm over and basal part leaf number 0 to7. The leaves are variable, they are measured according to leaves incisions, and most of the included incision. The qualitative analysis of EOs was performed using gas chromatography-mass spectrometry. EOs had various chemical compositions. Major compounds were trans-2-Decenal, linalool, decanal, 2-Dodecenal, 13-Tetradecanal, 2-Undecenal.

• Wind and Structures
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• 제37권4호
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• pp.315-329
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• 2023

We investigated the wind characteristics in the near-surface layer during the landfall of Typhoon Mujigae (2015) based on observations from wind towers in the coastal areas of Guandong province. Typhoon Mujigae made landfall in this region from 01:00 UTC to 10:00 UTC on October 4, 2015. In the region influenced by the eyewall of the tropical cyclone, the horizontal wind speed was characterized by a double peak, the wind direction changed by >180°, the vertical wind speed increased by three to four times, and the angle of attack increased significantly to a maximum of 7°, exceeding the recommended values in current design criteria. The vertical wind profile may not conform to a power law distribution in the near-surface layer in the region impacted by the eyewall and spiral rainband. The gust factors were relatively dispersed when the horizontal wind speed was small and tended to a smaller value and became more stable with an increase in the horizontal wind speed. The variation in the gust factors was the combined result of the height, wind direction, and circulation systems of the tropical cyclone. The turbulence intensity and the downwind turbulence energy spectrum both increased notably in the eyewall and spiral rainband and no longer satisfied the assumption of isotropy in the inertial subrange and the -5/3 law. This result was more significant in the eyewall area than in the spiral rainband. These results provide a reference for forecasting tropical cyclones, wind-resistant design, and hazard prevention in coastal areas of China to reduce the damage caused by high winds induced by tropical cyclones.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.65-66
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• 2021

Glycyrrhiza species (Licorice) are one of the most commonly used medicinal plants in Asian countries such as China, India and Korea. It has been traditionally used to treat many disease including cough, cold, asthma, fatigue, gastritis and respiratory tract infections. Glycyrrhiza new variety, Wongam (WG), have been developed by Korea Rural Development Administration and revealed several pharmacological effects. However, limited data are available on the potential adverse effects of the WG. Here, we evaluated the general toxicity of the WG extract through single oral dose toxicity study in Sprague-Dawley rats. After single oral dose administration, there was no mortality up to 5000 mg/kg during experiment period. In addition, there was no clinical signs including body weight change, gross findings and necropsy findings up to 5000 mg/kg during experiment period. To conclude, the no-observed-adverse-effect level (NOAEL) of WG was higher than 5000 mg/kg and no target organs were identified in male and female Sprague-Dawley rats.

• 통합자연과학논문집
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• 제17권1호
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• pp.1-12
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• 2024

Many abnormal climate events are occurring around the world. The cause of abnormal climate is related to temperature. Factors that affect temperature include excessive emissions of carbon and greenhouse gases from a global perspective, and air circulation from a local perspective. Due to the air circulation, many abnormal climate phenomena such as abnormally high temperature and abnormally low temperature are occurring in certain areas, which can cause very serious human damage. Therefore, the problem of abnormal temperature should not be approached only as a case of climate change, but should be studied as a new category of climate crisis. In this study, we proposed a model for the classification of abnormal temperature using random forests based on various meteorological data such as longitudinal observations, yellow dust, ultraviolet radiation from 2018 to 2022 for each region in Korea. Here, the meteorological data had an imbalance problem, so the imbalance problem was solved by oversampling. As a result, we found that the variables affecting abnormal temperature are different in different regions. In particular, the central and southern regions are influenced by high pressure (Mainland China, Siberian high pressure, and North Pacific high pressure) due to their regional characteristics, so pressure-related variables had a significant impact on the classification of abnormal temperature. This suggests that a regional approach can be taken to predict abnormal temperatures from the surrounding meteorological environment. In addition, in the event of an abnormal temperature, it seems that it is possible to take preventive measures in advance according to regional characteristics.