Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Isolation of feline panleukopenia virus from Yanji of China and molecular epidemiology from 2021 to 2022

  • Haowen Xue (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Chunyi Hu (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Haoyuan Ma (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Yanhao Song (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Kunru Zhu (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Jingfeng Fu (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Biying Mu (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University) ;
  • Xu Gao (Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, Agriculture College, Yanbian University)
  • Received : 2022.07.22
  • Accepted : 2022.11.23
  • Published : 2023.03.31
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Abstract

Background: Feline panleukopenia virus (FPV) is a widespread and highly infectious pathogen in cats with a high mortality rate. Although Yanji has a developed cat breeding industry, the variation of FPV locally is still unclear. Objectives: This study aimed to isolate and investigate the epidemiology of FPV in Yanji between 2021 and 2022. Methods: A strain of FPV was isolated from F81 cells. Cats suspected of FPV infection (n = 80) between 2021 and 2022 from Yanji were enrolled in this study. The capsid protein 2 (VP2) of FPV was amplified. It was cloned into the pMD-19T vector and transformed into a competent Escherichia coli strain. The positive colonies were analyzed via VP2 Sanger sequencing. A phylogenetic analysis based on a VP2 coding sequence was performed to identify the genetic relationships between the strains. Results: An FPV strain named YBYJ-1 was successfully isolated. The virus diameter was approximately 20-24 nm, 50% tissue culture infectious dose = 1 × 10-4.94/mL, which caused cytopathic effect in F81 cells. The epidemiological survey from 2021 to 2022 showed that 27 of the 80 samples were FPV-positive. Additionally, three strains positive for CPV-2c were unexpectedly found. Phylogenetic analysis showed that most of the 27 FPV strains belonged to the same group, and no mutations were found in the critical amino acids. Conclusions: A local FPV strain named YBYJ-1 was successfully isolated. There was no critical mutation in FPV in Yanji, but some cases with CPV-2c infected cats were identified.

Keywords

Acknowledgement

This study was supported by Natural Science Foundation (20210101368JC) and "13th Five-Year" Science and Technology Project of Education Department of Jilin Province (JJKH20200521KJ) of Yanbian University, China.

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