• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.87-91
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• 2005

Although 2D-PAGE has been widely used as the primary method for protein separation, difficulties in displaying proteins with an extreme values of isoelectric paint (pI), molecular size and hydrophobicity limit the technique. In addition, time consuming steps involving protein transfer and extraction from the gel-pieces can result in sample loss. Here, we describe a novel protein separation technique with capillary size-exclusion chromatography (CSEC) for rapid protein identification from human plasma. The method includes protein fractionation along with molecular size followed by in-solution tryptic digestion and peptide analysis through reversed phase liquid chromatography (RPLC) coupled to nanoflow electrospray-tandem mass spectrometry (ESI-MS/MS). Tryptic peptides are applied an a $100\;{\mu}m\;i.d.{\times}10mm$ length pre-column and then separated on a $75\;{\mu}m{\times}200mm$ analytical column at -100 nL/min flaw rate. Proteins were identified over the wide ranges of pI (3.7-12.3) when this technique was applied to the analysis of $1-2\;{\mu}L$ of human plasma. This gel-free system provides fast fractionation and may be considered a complementary technique to SDS-PAGE in proteomics.

• Structural Engineering and Mechanics
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• v.61 no.3
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• pp.359-370
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• 2017

This study presents a particle swarm optimization algorithm integrated with weighted particle concept and improved fly-back technique. The rationale behind this integration is to utilize the affirmative properties of these new terms to improve the search capability of the standard particle swarm optimizer. Improved fly-back technique introduced in this study can be a proper alternative for widely used penalty functions to handle existing constraints. This technique emphasizes the role of the weighted particle on escaping from trapping into local optimum(s) by utilizing a recursive procedure. On the other hand, it guaranties the feasibility of the final solution by rejecting infeasible solutions throughout the optimization process. Additionally, in contrast with penalty method, the improved fly-back technique does not contain any adjustable terms, thus it does not inflict any extra ad hoc parameters to the main optimizer algorithm. The improved fly-back approach, as independent unit, can easily be integrated with other optimizers to handle the constraints. Consequently, to evaluate the performance of the proposed method on solving the truss weight minimization problems with discrete variables, several benchmark examples taken from the technical literature are examined using the presented method. The results obtained are comparatively reported through proper graphs and tables. Based on the results acquired in this study, it can be stated that the proposed method (integrated particle swarm optimizer, iPSO) is competitive with other metaheuristic algorithms in solving this class of truss optimization problems.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.1 no.2
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• pp.1-14
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• 1991

In growing single crystals, which exhibit incongruent melting behavor, it is extremely difficult to maintain the stoichiometry of its chemical compositions for given crystals. For instance, $LiNbO_3$ is a typical one which exhibits such incongruent melt, especially with a large solid solution region that makes it difficult to maintain the chemical compositions. Such a variation can then cause a serious problem for the practical applications in designing a precision electro /optic device. Of the known methods of determining its composition and quality, an optical technique of measuring refractive index of the crystals has been implemented. This technique is also capable of determining optical uniformity of the grown crystals and the chemical compositions. The technique used for such characterizations is herein described and some of results are discussed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.4
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• pp.847-856
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• 2013

This paper presents an AP placement technique considering indoor location-awareness and examines its performance. The proposed AP placement technique is addressed from three performance metrics: location-awareness and AP-based wireless network performance as well as its cost. The proposed AP placement technique consists of meta-heuristic algorithms that yield a near optimal AP configuration for given performance metrics, and deterministic algorithms that improve the fast convergence of the near optimal AP configuration. The performance of the AP placement technique presented in this paper is measured under the environments simulating indoor space, and numerical results obtained by experimental evaluation yield the fast convergence of a near-optimal solution to a given performance metric.

• Journal of Power Electronics
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• v.17 no.5
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• pp.1186-1194
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• 2017

This paper proposes an improved low frequency Selective Harmonic Mitigation-PWM (SHM-PWM) technique. The proposed method mitigates the low order harmonics of the output voltage up to the $50^{th}$ harmonic well and satisfies the grid codes EN 50160 and CIGRE-WG 36-05. Using a modified criterion for the switching angles, the range of the modulation index for non-linear SHM equations is improved, without increasing the switching frequency of the CHB converter. Due to the low switching frequency of the CHB converter, mitigating the harmonics of the converter up to the $50^{th}$ order and finding a wider modulation index range, the size and cost of the passive filters can be significantly reduced with the proposed technique. Therefore, the proposed technique is more efficient than the conventional SHM-PWM. To verify the effectiveness of the proposed method, a 7-level Cascaded H-bridge (CHB) converter is utilized for the study. Simulation and experimental results confirm the validity of the above claims.

• Toxicological Research
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• v.23 no.3
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• pp.239-244
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• 2007

Respirable particles cause many occupational and environmental diseases of the lung. To study these diseases, laboratory animals are often exposed to these particles. Inhalation and instillation are the well-known techniques for experimental exposures of the lung to respirable particles. Recently, another technique called oropharyngeal aspiration (OPA) has been introduced for exposing the lung to pathogens and/or particles. The conventional OPA technique for the mouse is generally carried out using a micropipette with a fixed slant board. In order to modify the conventional OPA in this study, anesthetized mice were placed on an adjustable slant board, a syringe pump was used to deliver the solution to the oropharynx, and the mice were allowed to recover in vertically positioned tubes for 6 minutes until fully awaked. Most importantly, the whole process of OPA could be carried out simply by an examiner. This modified OPA technique was validated by exposing the mouse lung to Evans Blue dye with a success rate of 95%.

• Journal of Internet Computing and Services
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• v.6 no.2
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• pp.13-24
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• 2005

Software development by component integration is the mainstream for time-to-market and is the solution for overcoming the short lifecycle of software. Therefore, the effective techniques for component integration have been working. However, the systematic and practical technique has not been proposed. One of main issues for component integration is how to specify integration and the component architecture for operating the specification, in this paper, we propose a workflow variability design technique for component integration, This technique focuses on designing to a connection contract based on the component architecture. The connection contract is designed to use the provided interface of component and the architecture can assemble and customize components by the connection contract dynamically.

• Journal of Periodontal and Implant Science
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• v.27 no.1
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• pp.263-290
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• 1997

Homogenization technique is employed to investigate the series of stress analyses of mandible for three different types of dental implants. This technique helps to make proper material model of bone and analyze such a non homogeneous structure at the level of individual microstructural unit. The stress analyses with homogenization technique show much higher stress level in the sponge bone, compared to those of conventional FEM. It also manifested that even a minor lateral force results in crucial stresses in the dental implant system and that the macroscale model should take the shape and size after real mandible to produce reasonable solution in the analyses of dental implant systems. The shapes of dental implants simulated in this study are rectangular-cross-sectioned type, hemi-sphere rooted type, and wedge type implant. The stress states of mandible with hemisphere rooted type implant and wedge type implant show similar levels, while those with sectioned rectangular implant results in higher stresses. It is suggested that the distance between the implant tip and cortical bone be kept far enough to prevent stress concentrations in the mandible.

• The Journal of the Korean dental association
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• v.56 no.7
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• pp.366-374
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• 2018

Purpose : The purpose of this study was to compare the marginal fit of monolithic zirconia restorations fabricated form the conventional and the digital impression technique. Materials and methods : Ten patients were restored with monolithic zirconia restorations fabricated from the digital and the conventional impression technique. Before definitive insertion, silicone replicas were produced from all twenty crowns. The silicone replicas were cut in three sections; each section was evaluated at two points. The measurement was carried out by using a measuring microscope (Olympus BX 51) and I-Solution. Data from the silicone replica scores were analyzed by Shapiro-Wilk test for normal distribution and the t-test for equality of two population's mean. All tests were performed with ${\alpha}-level$ of 0.05. Results : The average marginal gaps of monolithic zirconia restorations were $133.81{\pm}36.46{\mu}m$ for the conventional impression technique, $90.07{\pm}9.47{\mu}m$ for the digital impression technique. No statistical differences were found between the two impression techniques Conclusion : Both prostheses presented clinically acceptable results with comparing the marginal fit.

• Journal of Electrochemical Science and Technology
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• v.1 no.1
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• pp.45-49
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• 2010

$Li_2PtO_3$ thin film electrodes, which might be possible candidate for the cathode materials for implantable batteries, were synthesized using an electrostatic spray deposition (ESD) technique onto a platinum foil substrate. Single phase $Li_2PtO_3$with a structure similar to layered $LiCoO_2$ structure were synthesized by spraying a precursor solution of $CH_3CO_2Li2H_2O$ in ethanol onto a Pt substrate at temperatures ranging from 200 to $400^{\circ}C$ followed by annealing at above $600^{\circ}C$. Lithium carbonate was the only major phase at temperatures up to $500^{\circ}C$. The X-ray diffraction (XRD) peaks of the Pt foil substrate and lithium carbonate disappeared at temperatures >$600^{\circ}C$. The volumetric capacity of the $Li_2PtO_3$ thin film synthesized using the ESD technique was approximately 817 mAh/$cm^3$, which exceeded that of $LiCoO_2$ (711 mAh/$cm^3$).