• Food Science of Animal Resources
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• v.43 no.6
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• pp.989-1001
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• 2023

Processed foods containing pork fat tissue to improve flavor and gain economic benefit may cause severe issues for Muslims, Jews, and vegetarians. This study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) based on a monoclonal antibody specific to thermal stable-soluble protein in pork fat tissue and apply it to detect pork fat tissue in heat-processed (autoclave, steam, roast, and fry) beef meatballs. To develop a sensitive iELISA, the optimal sample pre-cooking time, coating conditions, primary and secondary dilution time, and various buffer systems were tested. The change in the iELISA sensitivity with different 96-well microtiter microplates was confirmed. The detection limit of iELISA performed with an appropriate microplate was 0.015% (w/w) pork fat in raw and heat-treated beef. No cross-reactions to other meats or fats were shown. These results mean that the iELISA can be used as an analytical method to detect trace amounts of pork fat mixed in beef.

• The Korean Journal of Mycology
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• v.13 no.4
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• pp.235-241
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• 1985

The present study was designed to investigate biosynthetic patterns of polysaccharides under catabolic repression and derepression in Saccharomyces uvarum. Correlation coefficients between polysaccharide synthesis and polyphosphate accumulation were examined, according to the culture phase and under various phosphate concentrations (free, limited, sufficient). During catabolic derepression, biosynthesis of glycogen was enhanced. rapidly and highly in the cells grown on minimal medium, compared with those grown on the complete medium. Acid soluble glycogen type was the main component of total glycogen and alkali soluble glycogen was synthesized in small amount, after 24 hr culture, at the time of almost exhaustion of sugar in the medium. Total glycogen was accumulated highly in proportion to the amount of phosphate added to the medium. It could be postulated that type 'C' isoenzyme among ALPase was directly or indirectly correlated with the glucan synthesis. Mannan synthesis indicated maximal amount at the early exponential phase and stationary phase, and also acid soluble sugars at the stationary phase. Correlation coefficient between the mannan synthesis and poly-p-'C' accumulation, and also between mannan synthesis and phospholipid content indicated 0.866 and 0.726, respectively.

• Journal of the Korean Ceramic Society
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• v.26 no.5
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• pp.698-704
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• 1989

Basic investigation for the flexural strength and water stability of hardened cement pastes using ordinary portland cement with water-soluble polyer (hydroxypropyl methyl cellulose ; HPMC) was carried out with 0.2 of water cement ratio. For molding of the specimen, the paste was mixed by twin roll mill. According to increase in the content of HPMC, the setting time of cement paste was delayed and the flexural strength was increased. The maximum flexural strength of hardened cement paste with 5.0wt% of HPMC was about 330 kg/$\textrm{cm}^2$. The expansion of the hardened cement paste immersed in water was increased with the content of water soluble polymer(HPMC). Consequently, the strength and the water stability of the hardened cement pastes were remarkably reduced by the expansion of them.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.10 no.3
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• pp.87-93
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• 2011

CNC lathe machining has been widely used for parts machining of vehicles, aircraft, ships, electronics, etc. because cost savings for shortening processing time and increasing productivity are great. In this study, the purpose is to investigate the effect of cooling methods such as oil mist, water-soluble cutting oils on the workpiece surface roughness with the cutting speed, cutting depth, tool nose radius and feed rate of CNC lathe machine as a parameter in the cutting process of the aluminum alloy 2024 which is used a lot recently on aircraft parts. It is found that oil mist is coolant and water-soluble cooled by cutting the experimental conditions, cutting speed and cutting depth without effecting the surface roughness value was constant.

• The Journal of Korean Academy of Prosthodontics
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• v.9 no.1
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• pp.41-45
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• 1969

A major disadvantage of Dental cements is their solubility. So it is very important to measure the exact amount of solubility to select clinically suitable materials. The most common laboratory test for solubility is the measurement of disintegration in distilled water, as outlined in A.D.A. Specifications 8 and 9, In addition to the possible factors influencing the solubility, the experiments were all conducted in compliance with A.D.A. Specifications. The solubility of 2 Zinc Phosphate cements and 1 Silicate cement in time of dissolution, concentration of solute in dissolving medium, and type of dissolving medium were investigated. The following results were obtained. 1. Generally the materials were more soluble in organic acids than in distilled water. 2. The dissolution cements tends be minimized by tests utilizing prolonged storage in the same media. 3. In Acetic acid solution, Zinc Phosphate cements were more soluble than Silicate cement, and in Citric acid solution both were markedly more soluble. 4. Solubility was increased by continually presenting fresh liquid, unsaturated with solute, to the cement-water interface.

• Journal of Industrial Technology
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• v.28 no.B
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• pp.59-63
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• 2008

Rapid production of therapeutic proteins such as angiostatin and endostatin angiogenic inhibititors has been highly demanded for cancer treatment. In this regard, recombinant human angiostatin and endostatin were successfully expressed as soluble forms by maltose binding protein (MBP)-mediated fusion expression in Escherichia coli. PCR amplified, angiostatin and endostatin genes from human placenta cDNA library were inserted into an expression vector pMAL-c2e to construct prokaryotic expression vectors, pMAL-c2e/AS and pMAL-c2e/ES, respectively. Recombinant angiostatin and endostatin were efficiently expressed in E. coli origami (DE3) after IPTG induction and protein expression were confirmed by SDS-PAGE analyses. The expressed recombinant proteins were purified near homogenity using an amylose affinty column chromatography. In contrast that previous E. coli expressions were all insoluble, our results first time demonstrated that MBP fused human angiostatin and endostatin were soluble in E. coli.

• YAKHAK HOEJI
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• v.28 no.4
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• pp.231-235
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• 1984

Active principles for the anti-fatigue activity of Panax ginseng were studied in mice using the swimming performance method. Ginseng water extract maximized the prolongation of swimming time 18 hours after administration. The potencies of anti-fatigue activities were found as in the order of ether soluble fraction and butanol soluble fraction as those of antioxidant activities previously determined. The anti-oxidant components, maltol, salicylic acid and vanillic acid isolated from the ether soluble fraction of Panax ginseng strongly exhibited the antifatigue activities, where as highly purified crystalline ginsenoside $4Rb_1$, Re and $4Rg_1$ did not.

• Korean journal of food and cookery science
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• v.30 no.3
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• pp.351-359
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• 2014

The application of persimmon for making new products was investigated by analyzing the degree of astringency removal, softness of fruit, and sensory properties of Daebong persimmons (Diospyros kaki L) treated with three different astringency removal methods of steeping in water, steeping in 1% salt solution, and vacuum packing at $40^{\circ}C$. The weight of persimmon fruit increased with increasing steeping time, but decreased with increasing vacuum packing time. The fruit steeped in $40^{\circ}C$ salt solution (1%, w/w) was not detected with soluble tannin and not developed color with ferric chloride after steeping for 9 h. A total of 12 h of steeping time was required for removing soluble tannin using other methods. The lightness (L) in color and hardness in texture decreased when storage time increased, regardless of astringency removal methods. In overall, the preference was shown with the highest score in persimmon steeped in salt solution for 9 h and 12 h that were vacuum packed at $40^{\circ}C$. In conclusion, the astringency removal condition of Daebong persimmon was good after being steeped on 1% salt solution for 9 h at $40^{\circ}C$.

• Safety and Health at Work
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• v.3 no.1
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• pp.1-10
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• 2012

The aim of this review was to assess current knowledge related to the occupational exposure limit (OEL) for fluid aerosols including either mineral or chemical oil that are generated in metalworking operations, and to discuss whether their OEL can be appropriately used to prevent several health risks that may vary among metalworking fluid (MWF) types. The OEL (time-weighted average; 5 mg/$m^3$, short-term exposure limit ; 15 mg/$m^3$) has been applied to MWF aerosols without consideration of different fluid aerosol-size fractions. The OEL, is also based on the assumption that there are no significant differences in risk among fluid types, which may be contentious. Particularly, the health risks from exposure to water-soluble fluids may not have been sufficiently considered. Although adoption of The National Institute for Occupational Safety and Health's recommended exposure limit for MWF aerosol (0.5 mg/$m^3$ ) would be an effective step towards minimizing and evaluating the upper respiratory irritation that may be caused by neat or diluted MWF, this would fail to address the hazards (e.g., asthma and hypersensitivity pneumonitis) caused by microbial contaminants generated only by the use of water-soluble fluids. The absence of an OEL for the water-soluble fluids used in approximately 80-90 % of all applicants may result in limitations of the protection from health risks caused by exposure to those fluids.

• Membrane Journal
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• v.8 no.3
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• pp.170-176
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• 1998

A study on the bioconversion of soluble starch to cyclodextrin(CD) homologue by CGTase was performed in the membrane-enzyme reactor equipped with a dead-end type membrane module. in the batch reactor, the total conversion of soluble starch to CD homologue was decreased rapidly from a maximum value of 45 % with increasing reaction time due to the product inhibition and breakdown of CD homologue to the reducing sugars. However, in the membrane-enzyme reactor, the total conversion of soluble starch was maintained at a constant value of 35 % throughout the reaction, since the membrane(MWCO = 10,000) promptly separated CD homologue from the reaction mixture. After the macdon for 24 hr in the membrane-enzyme reactor using a 10 % soluble starch solution, the cumulative production amount of CD homologue was about 3.7 kg/m$^2$ at the operating pressure of 2 atm.