• 제목/요약/키워드: Soluble group

검색결과 688건 처리시간 0.033초

천마 분획물이 고지방식이를 급여한 흰쥐의 혈청 지질농도에 미치는 영향 (Effect of Gastrodiae rhizoma Fractions on Serum Lipid Concentrations in Rats Fed with High Fat Diet)

  • 홍희도;김영찬;금인경;김성수;김경임;한찬규
    • Applied Biological Chemistry
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    • 제48권4호
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    • pp.370-374
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    • 2005
  • 건강식품소재로서 천마의 효능과 활용방안을 모색하고자 고지방식이를 급여한 흰쥐에게 천마성분 분획물이 혈청 지질함량과 동맥경화유발지수 등에 미치는 영향을 조사하였다. 시험기간은 총 12주간으로 실험 1기는 초기 6주 동안 흰쥐사료에 유지를 첨가한 고지방식이(HFCD)를 급여하였고, 실험 2기는 6주 동안 천마성분 분획물을 함께 투여하였다. 천마성분 분획물로는 에탄올 가용성인 저분자분획(MW<1K, GR-1), 수용성 획분에 pronase처리한 다당체분획(GR-2), periodate를 이용한 다당체산화를 통하여 단백질분획(GR-3)을 조제하여 사용하였다. 시험 종료체중은 고지방대조군이 천마분획물군 보다 평균 44 g 정도 높았지만 통계적인 차이는 없었으며 증체량, 식이섭취량 및 식이효율(FER) 역시 유의적인 차이가 없었다. 실험 2기 종료후 측정한 혈청 지질중 TC와 TG농도는 다당체 분획을 투여한 경우에 고지방대조군에 비해 각각 21.5, 39.6% 낮았다. HDL콜레스테롤은 단백질분획이 저분자분획과 다당체분획 보다 높았고, LDL콜레스테롤은 천마분획물 투여군에서 고지방대조군 보다 유의하게 낮았다. 동맥경화위험지수는 단백질분획이 1.45로 다른 천마성분 분획물들의 $1.94{\sim}2.05$, 대조군의 2.12에 비해 가장 낮았다. 따라서 천마 다당체 또는 단백질분획 같은 수용성 고분자분획은 혈중 지질함량 및 조성을 개선시킬 수 있는 기능성 식품소재로 활용될 수 있을 것으로 사료된다.

수산화칼슘 엽면살포가 '대월' 복숭아의 신초 생육 및 과실 품질에 미치는 영향 (Effects of Foliar Spray of Calcium Hydroxide on Shoot Growth and Fruit Quality in 'Daewol' Peach (Prunus persica Batsch))

  • 박지영;손인창;김대일
    • 한국환경농학회지
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    • 제29권2호
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    • pp.102-108
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    • 2010
  • 과실비대기 중에 수산화칼슘의 엽면살포가 '대월' 복숭아의 수체생육 및 과실품질에 미치는 영향을 구명하고자 실험을 수행하였다. 적과 후 수산화칼슘 1, 2, 5, 10 $g{\cdot}L^{-1}$을 일주일 간격으로 엽면살포한 결과, 신초생장은 대조구에 비해 현저히 억제되었다. 신초 기부로부터 5번째 잎의 엽색도를 측정한 결과, 수산화칼슘 엽면살포구에서 평균 42.1 SCDSV로 대조구의 40.9 SCDSV보다 높게 나타났으며, 광합성률은 수산화칼슘을 고농도로 처리할수록 크게 증가하였다. 과실 품질을 조사한 결과, 과중은 고농도의 수산화칼슘을 처리할 수록 유의하게 증가하였다. 당 함량은 대조구가 8.78 $^{\circ}Brix$로 가장 낮았던 반면, 수산화칼슘 처리구에서 9.17, 9.22, 9.71, 10.58 $^{\circ}Brix$로 고농도로 처리할수록 증가하였다. 산도와 안토시아닌 함량은 모든 처리구에서 유의성이 인정되지 않았으며 과피와 과육의 경도는 고농도의 수산화칼슘 처리에 의해 유의하게 증가하였다. 잎의 형태적 관찰 결과, 대조구의 책상조직(63.5 ${\mu}m$)에 비하여, 수산화칼슘 처리구는 86.5, 87.5, 93.6, 107.4 ${\mu}m$로 처리농도가 높을수록 조직이 건전하게 유지되고 잎의 두께 또한 증가하였다. 과실의 표피와 하피의 세포벽을 관찰한 결과, 수산화칼슘 엽면살포에 의해 세포벽의 두께가 증가하는 것을 확인할 수 있었다.

Bifidobacterium longum HY8001 균주의 Escherichia coli O157:H7과 Salmonella typhimurium DT104 장곤 내 감염 예방효과 및 Vero cytotoxin 중화효과 (Prophylactic Effects of Bifidobacterium longum HY8001 against Escherichia coli O157:H7 and Salmonella typhimurium DT104 Enteric Infection and Evaluation of Vero Cytotxin Neutralizing Effects)

  • 양수진;윤장원;서근석;구혜정;김소현;배형석;백영진;박용호
    • 한국미생물·생명공학회지
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    • 제27권5호
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    • pp.419-425
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    • 1999
  • Prophylactic effects of Bifidobacterium longum HY8001, Korean isolate, against Escherichia coli O157:H7 and Salmonella typhimurium DT104 enteric infection were examined at four groups of specific pathogen free(SPF)-ICR mouse for each pathogen. B. longum HY8001+B. typhimurium DT104+B. longum HY8001(BL+ST+BL) group and B. longum HY8001+E. coli O157:H7+B. longum HY8001(BL+E+BL) group were fed with B. longum HY8001 before and after E. coli O157:H7 or s. typhimurium DT104 challenge, while B. longum HY8001+S. typhimurium DT104(BL+ST) and B. longum HY8001+e. coli O157:H7(BL+E) groups were fed with B. longum HY8001 only before E. coli O157:H7 or S. typhimurium DT104 challenge. E. coli O157:H7(E) and S. typhimurium DT104(ST) groups were challenged with each pathogen without B. longum HY8001 administration and control groups were administered with phosphate buffered solution(PBS). After the oral administration with B. longum HY8001(109cfu), th emice were challenged with E. coli O157:H7(2$\times$1010cfu) or S. typhimurium DT104(108cfu) and the mortality rate and the fecal shedding of challenged pathogen were also examined define the reactivity of the B. longum HY8001. Production of toxin neutralizing substance(s) of B. longum HY8001 was determined by cell cytotoxicity assay using Vero cells. Fecal shedding of th eS. typhimurium DT104 was significantly decreased in BL+ST+BL group fed with B. longum HY8--1 before and after challenge(p<0.05), while the fecal shedding s of S. typhimurium DT104 in BL+ST and St groups remained more than 106cfu. the protective effect of the B. longum HY8001 against E. coli O157:H7 was significantly high only in BL+E+BL group fed with b. longum Hy8001 before and after E. coli O157:H7 challenge from the result of fecal E. coli O157:H7 isolation rate, mortality rate, and intestinal contents culture to detect E. coli O157:H7. the mortality rate of the BL+e and E groups. The cytopathic effect (CPE) of the Vero cytotoxin (Shiga like toxin I & II) in Vero cell was neutralized in B. longum HY8001 culture supernatant added wells which indicate the presence of soluble Vero cytotxin neutralizing substance(s) in B. longum HY8001 culture suprnatant.

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배추 폐기물로부터 분리한 불용성 식이섬유가 고지방 식이를 급여한 쥐의 혈중 지질농도에 미치는 영향 (The Effect of Insoluble Dietary Fiber Extracted from Chinese Cabbage Waste on Plasma Lipid Profiles in Rats Fed a High Fat Diet)

  • 유문려;고강희;김학렬;김인철
    • 한국식품영양과학회지
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    • 제41권1호
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    • pp.33-40
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    • 2012
  • 농산물 유통과정 및 김치 제조과정 중 발생하는 배추 폐기물의 이용성을 확대하기 위해 이로부터 불용성 식이섬유를 분리하고 혈중 콜레스테롤 저하능을 확인하였다. 배추폐기물을 $121^{\circ}C$/15분 조건에서 추출한 다음, 수용성 물질은 제거하고 남은 박에 전분 및 단백질 분해효소를 처리하여 효소처리된 불용성 식이섬유 분말(EIDF)을 제조하였다. 고지혈증 및 동맥경화에 미치는 영향을 알아보기 위해 고지방 식이에 0.1~1%의 EIDF를 첨가하여 이를 흰쥐에 4주 동안 공급하였다. 체중증가량에 대해 p<0.05 수준에서는 유의적인 차이를 확인할 수 없었으나 p<0.1 수준에서는 고지방 식이를 한 경우보다 EIDF를 첨가한 군의 체중이 낮았다. 또한 EIDF는 혈중 중성지방 및 총 콜레스테롤, LDL-콜레스테롤, VLDL-콜레스테롤의 수준을 낮췄으며, HDL-콜레스테롤 함량은 증가시켜 동맥경화지수가 낮은 것으로 확인되었다. 특히 EIDF를 투여함에 따라 LDL-콜레스테롤 함량을 2주째 에 66.7%~72.8%, 4주째에 45.1%~56.5%까지 낮춰 배추 폐기물에서 추출한 불용성 식이섬유의 혈중 콜레스테롤 저하능이 우수한 것을 평가할 수 있었으며, 이를 이용하여 부가 가치가 높은 건강기능보조식품으로의 활용이 기대된다.

쇠비름(Portulaca oleracea L.) 추출물의 탄수화물 소화 효소 저해와 식후 고혈당 완화 효과 (Portulaca oleracea L. Extract Lowers Postprandial Hyperglycemia by Inhibiting Carbohydrate-digesting Enzymes)

  • 박재은;한지숙
    • 생명과학회지
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    • 제28권4호
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    • pp.421-428
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    • 2018
  • 식후고혈당은 제 2형 당뇨병의 발병에 부정적인 영향을 미치고 미세혈관 및 대혈관 질환 등의 당뇨병 합병증 유발과 밀접한 관계가 있다. 따라서 식후고혈당을 조절하는 것이 당뇨병 합병증의 위험을 줄이는 가장 중요한 요소이다. 식후고혈당은 소장에서 ${\alpha}$-글루코시다아제와 같은 탄수화물 소화 효소를 저해함으로써 조절될 수 있다. 이에 본 연구는 쇠비름 에탄올 추출물(PEE)과 물 추출물(PWE)이 탄수화물 소화 효소를 저해하고, 당뇨병 마우스에서 식후 고혈당을 강하시키는 효과에 대해 조사하였다. ${\alpha}$-글루코시다아제와 ${\alpha}$-아밀라아제에 대한 저해효과는 두 추출물 모두 양성대조군인 acarbose보다 더 효과적이었으며, PEE에 의한 ${\alpha}$-글루코시다아제 저해 효과가 PWE 보다 더 효과적이었다. Diabetic mice에 전분(2 g/kg)을 투여한 후의 혈당 증가는 30, 60, 120분에 각각 383.7, 429.3, 360.2 mg/dL로 나타났고, 전분(2 g/kg)과 PEE 또는 PWE 추출물(300 mg/Kg)을 투여한 후의 혈당 증가는 30, 60, 120분에 각각 337.0, 368.5, 290.1 mg/dL과 365.8, 379.2, 324.3 mg/dL로 나타나, PEE 추출물 투여군이 대조군에 비해 식후 혈당 강하가 효과적으로 나타남을 알 수 있었다. 이러한 결과는 쇠비름 추출물이 탄수화물 소화효소를 저해함으로써 식후 고혈당을 완화시키고, 특히 쇠비름 에탄올 추출물(PEE)이 쇠비름 물 추출물(PWE) 보다 식후 고혈당을 완화시키는데 더욱 효과가 있는 것으로 나타났다.

한반도의 황사 관측현황 및 배경지역 미세먼지의 화학적 조성에 관한 연구 (Study on the Yellow Sandy Dust Phenomena in Korean Peninsula and Chemical Compositions in Fine Particles at Background Sites of Korea.)

  • 백광욱;정진도
    • 환경위생공학
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    • 제19권4호
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    • pp.9-18
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    • 2004
  • In this study, the observation data for the yellow sandy dust phenomena from the year 1999 to 2003 at background sites in Korea were collected at Global Atmospheric Observatory at An-Myeon island and its temporal variation were analyzed. The chemical characteristics of the fine particles were also analyzed in order to evaluate sources of the yellow sandy dust particles. The results showed that the monthly average mass concentration of the fine particles was the highest in springtime and the lowest in summertime in general. The magnitude of its variation was also the highest in March in which the occurrence of yellow sandy dust was the most frequent and thus the number of samples was the largest, while the lowest in June through September. The yearly variation of ion components contributions to the total mass concentration of the fine particles was slowly decreasing, showing that $63\%$ in 1999, $59\%$ in 2000 and $56\%$ in 2003. The most prevalent ion components in the fine particles were found to be $NO_3$ and $SO_4^{2-}$, which are known to be source materials of acidic precipitation, and $NH_4^+$, a neutralizing material of the acid precipitation. Relative proportion of metal components in the fine particles was calculated as $14\%$ in average, and their concentrations are in an order of Fe > Al > Na > Ca > Zn > Pb > Cu > Mn > Ni > Cd > Cr > Co > U. The results indicated that main sources of the metals was soil-originated Fe, Al, Ca, and Mg, and the contribution of anthropogenic air Pollution-originated Zn, Pb, Cu, Mn were also high and keep slightly increasing. Statistical analysis showed that the chemical components could be divided into soil-originated group of Mg, Al, Ca, Fe, and Mn and air pollution-originated group of $NO_3$, Zn, Pb, and they are occupying more than $60\%$of all the components in the dusty sand. The results explain that An-Myeon island is more influenced by soil-originated source than ocean-originated one and also the influencing strength of anthropogenic poilution-originated source is less than $50\%$ of that of soil-originated sources. Compared to non-yellow sandy period, the yellow sandy dust period showed that the amounts of soil-originated $Mg^{2+}$ and $Ca^{2+}$ and ocean-originated $Na^+$ and $Cl^-$ were increased to more than double and the metals of Mg, Al, Ca, Fe were also highly increased, while micro metal components such as Pb, Cd, Zn, which have a tendency of concentrating in air, were either decreased or maintained at nearly constant level. In the period of yellow sandy dust, a strong positive correlation was observed between water soluble ions and between metals in terms of its concentration, respectively. Factor analysis showed that the first group being comprised of about $43\%$ of the total inorganic components was affected by soil and they are ions of $Na^+,\;Mg^{2+}\;and\;Ca^{2+}$ and metals of Na, Fe, Mn and Ni. The result also showed that the metals of Mg and Cr were classified as second group and they were also highly affected by soil sources.

Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic α-Amylase Activity in Korean Native Steer

  • Lee, S.B.;Choi, C.W.;Jin, Y.C.;Wang, T.;Lee, K.H.;Ku, M.B.;Hwang, J.H.;Kim, K.H.;Vega, R.S.A.;Lee, H.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권5호
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    • pp.654-660
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    • 2013
  • Three Korean native steers ($779{\pm}24$ kg) fitted with duodenal cannulas were used in a $3{\times}3$ Latin square design to investigate the influence of oral administration of soluble proteins, intact casein (IC) and acid hydrolyzed casein (AHC), on gastro-intestinal hormone (GIH) secretion in the blood and pancreatic ${\alpha}$-amylase activity in the duodenum. Oral treatment consisted of a basic diet (control), IC (C+100% protein), or AHC (C+80% amino acid, 20% peptide) for 21 d. Blood and duodenum samples were collected for measurement of serum GI hormones, and pancreatic ${\alpha}$-amylase activity was determined at 900, 1030, 1330, 1630, and 1930 h after feeding on d 21 of treatment. The levels of serum cholecystokinin (CCK) and secretin in the IC treatment group were higher compared to the other treatment groups (p<0.05). In addition to the changes in CCK and secretin levels upon IC treatment, the pancreatic ${\alpha}$-amylase activity in the duodenum was higher in the IC group compared to the control diet group (p<0.05). The response of serum ghrelin to IC and AHC treatment was in accordance with the response of serum secretin. The level of peptide fragments flowing in the duodenum was higher in the IC treatment group than the other treatment groups (p<0.05). In conclusion, this study demonstrated that an increase in duodenal CCK and secretin upon IC oral administration increased pancreatic ${\alpha}$-amylase secretion. In addition, ghrelin may be associated with GI hormone secretion in Korean native steers.

Determination of Carnitine Renal Threshold and Effect of Medium-Chain Triglycerides on Carnitine Profiles in Newborn Pigs

  • Heo, K.N.;Odle, J.;Lin, X.;van Kempen, T.A.T.G.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권2호
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    • pp.237-242
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    • 2001
  • Colostrum deprived, newborn pigs (N=12, $1.64{\pm}0.05kg$) were used to study the renal threshold of carnitine, and effects of emulsified medium-chain triglyceride (MCT, tri-8:0) feeding on kinetics of plasma carnitine and urinary carnitine excretion. An arterial catheter was inserted through an umbilical artery, and a bladder catheter was inserted via the urachus. Piglets were oro-gastrically gavaged with one of six carnitine levels (0, 60, 120, 180, 240, $480{\mu}mol/kg\;W^{0.75}$) with (+MCT) or without medium-chain triglycerides (-MCT) in 0.9% NaCl solution. Blood was sampled into heparinized tubes at 0, 1, 2, 4, 6, 8, 14, and 20 h after gavage, and urine was collected and pooled into 1 h or 2 h composite samples to determine free- and short-chain carnitine concentrations. Plasma from the 12 newborn piglets before gavage contained $10.6{\pm}1.2{\mu}mol/L$ free carnitine and $7.2{\pm}0.6{\mu}mol/L$ acid-soluble acyl carnitine. The renal threshold for carnitine was similar between the MCT and the +MCT group (42.6 13.1 and $46.4{\pm}2.0{\mu}mol/L$, respectively), but the correlation between plasma free carnitine and urinary excretion was altered. Plasma free carnitine linearly increased with increasing carnitine dosage (-MCT group, $R^2=0.95$, p<0.001; +MCT group, $R^2=0.91$, p<0.001), but was decreased by 50% when medium-chain triglycerides were fed. The peak in plasma free carnitine concentration was depressed by medium-chain triglycerides feeding also. Therefore, the plasma and urinary short-chain/free carnitine ratio of the +MCT group was increased by 100% and 40%, respectively (p<0.01). Feeding of medium-chain triglycerides may delay plasma carnitine elevation via altering the kinetics of absorption. Similarly, the plasma and urinary short-chain/free carnitine ratio were affected by interaction between medium-chain triglycerides and time (p<0.01). The present study suggests that an oral carnitine dose over $480{\mu}mol/kg\;W^{0.75}$ may be needed to reach the free carnitine renal threshold within a short period, especially when provided together with medium-chain triglyceride.

혈중지질에 대한 진피추출물의 유효성 평가를 위한 인체적용시험 : 무작위배정, 이중맹검 (Clinical Trial to Evaluate the Efficacy of Extract of Citri Pericarpium on Serum Lipid Profiles in Subjects: a Randomized, Double-blind)

  • 이진상;도은주;곽민아;박현진;하일도;성기준;김미려
    • 대한본초학회지
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    • 제26권1호
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    • pp.125-132
    • /
    • 2011
  • Objectives : This study was performed to evaluate the efficacy of extract from Citri Pericarpium (CP) on lipid-lowering effect in semi-healthy subjects with increased serum levels of total cholesterol and triglyceride. Methods : A experimental group (n=45) and placebo group (n=46) were randomly established based on the plasma lipid profiles (triglyceride, total cholesterol etc.) in the semi-healthy subjects, then all subjects randomly took experimental or placebo capsules, which contain 1,200 mg of CP or the same dose of soluble starch per day with regular meals for 8 weeks by double-blind method. Results : One-hundred forty (140) subjects were screened, then 91 subjects enrolled in the study. No serious adverse events were reported for trial period. The CP treatment showed statistically significant decrease in serum levels of triglyceride both at 4-weeks (p<0.01) and at 8-weeks (p=0.0001). Futhermore, there was significant difference in the triglyceride levels between CP-treated and placebo group. The CP-treated but not placebo group, experienced decrease in serum levels of total cholesterol at 8-weeks. Then there was no significant difference in the total cholesterol levels between the two groups. In addition, statistically significant difference of AST, ALT, ${\gamma}$-GT serum levels wasn't shown before and after trial in two groups. Conclusion : These results demonstrate that CP can significantly suppress the elevated triglyceride but not total cholesterol level. Therefore, CP may play a role in the improvement of blood lipid levels, as an effective functional food.

국산한약재 추출물을 이용한 전통 콩된장의 품질특성 (Quality Properties of Traditional Doenjang Supplemented with Extracts of Korean Herb Medicines.)

  • 박석규;정회정;김성희;권선화;이상원
    • 생명과학회지
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    • 제14권4호
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    • pp.553-559
    • /
    • 2004
  • 국산 한약재를 이용하여 식품학적 품질 및 기능성이 증진된 전통된장을 개발할 목적으로 여러 종류의 국산 한약재를I∼IV군으로 조합하여 121$^{\circ}C$에서 2시간 동안 추출물을 제조한 다음, 담금용 염수에 여러 농도로 첨가한 후 3개월 동안 자연발효시킨 한방 콩된장의 품질특성을 조사하였다. 한약재 첨가 된장의 일반성분 분석에서 대조구는 수분 61.9%, pH 5.50, 총산도 3.25 ml, 염도 12.6%를 나타내었으나 시험구는 수분 58.2% ∼61.9%, pH 5.32∼5.56, 총산도 3.07∼3.70 ml, 염도 11.7∼13.9%범위로 거의 비슷한 값을 나타내었으며, 환원당, 조단백, 조지방 및 아미노태 질소함량은 대조구보다 약간 높게 나타났다. 지용성 갈변도는 대조구와 큰 차이가 없었지만, 수용성 갈변도는 I군과 II군은 III군과 IV군에서 매우 높은 경향이었다. 한약재 첨가 된장의 색도 측정에서 명도(L)는 대조구 된장(31.72)에 비하여 I군과 II군의 된장이 낮은 수치(24.32∼27.71)를 나타내어 된장의 색깔이 검은 색을 띄었다. 적색도(a)는 제 I군을 제외한 나머지 된장에서는 대조구보다 약간 낮은 값을 나타내었으며, 황색도(b)는 III군과 IV군의 경우 대조구 된장과 비슷하였으나 I군과 II군은 낮게 나타났다.