• Title/Summary/Keyword: Soluble factor

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High Throughput Fluorogenic Assay for TNF-alpha Converting Enzyme(TACE) inhibitors

  • Keum, Se-Hoon;Lee, Bong-Yong
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.125.2-126
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    • 2003
  • Human tumor necrosis factor-alpha (TNFa) is a key pro-inflammatory cytokine produced by activated monocytes and macrophage as a part of the self-defence machinery. TNF-a converting enzyme (TACE) is the metalloproteinase that processes the membrane bound precursor of TNFa to the soluble component. (omitted)

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Correlation between Soluble Solid Content and Physicochemical Properties of ‘Bing’ Cherry at Different Stages of Ripening after Harvest (‘Bing’ 체리의 숙기에 따른 당도와 이화학적 품질인자의 상관관계)

  • Hong, Yoon-Pyo;Choi, Sun-Young;Cho, Mi-Ae;Choi, Sun-Tay;Kim, Sung-Jong
    • Food Science and Preservation
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    • v.17 no.3
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    • pp.370-375
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    • 2010
  • Cherries (Prunus avium L) were selected at the light red (LR) and dark red (DR) stages of maturation. Soluble solid content and the physicochemical properties of fresh 'Bing' cherries were analyzed to identify an instrumental nondestructive attribute reflecting changes in sweetness. Soluble solid content was significantly correlated with various physicochemical properties (firmness, color, and acid level) in LR-stage cherries. In DR-stage cherries, only firmness was positively correlated with soluble solid content. A positive correlation was found between soluble solid content and firmness of 200 randomly selected cherries. Thus, flesh firmness may be a useful quality factor indicating potential consumer acceptance of 'Bing' cherries.

Study on the Quantitative Analysis Methods of Hexavalent Chromium in Flexography Inks and Organic Pigments - Alkaline digestion and Colorimetric Measurement - (플렉소 잉크 및 유기안료의 6가 크롬 함량 측정 방법에 관한 연구 - 알칼리 분해 및 비색측정법 -)

  • Kim, Jin-Woo;Youn, Hye-Jung;Lee, Hak-Lae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.41 no.4
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    • pp.33-42
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    • 2009
  • This study was performed to evaluate the hexavalent chromium content in flexography inks and organic pigments used as colorants for the ink. The digestion of sample was carried out under alkaline condition, and the content of soluble hexavalent chromium extracted from samples was determined by UV-visible colorimetric analysis method after alkaline digestion. Duplicate sampling system to obtain two digested samples, was applied in this study. To determine the hexavalent chromium content in the flexography ink and organic pigment colorimeteric analysis was employed. Because the organic pigment is the main ingredient of flexography ink it is important to remove the errors associated with the colorant of the ink in colorimetric determination. The duplicate sampling system allowed us to correct the errors associated with the colorimeteric measurement. The additional filtration was found an essential step to exclude colorimeteric error derived from the various precipitates. The soluble hexavalent chromium content in flexography inks was generally less than 5 ppm. Yellow, violet and some magenta colors showed higher soluble hexavalent chromium contents. The content of hexavalent chromium in organic pigments was greater than flexography inks, and yellow, violet and some magenta pigments contained greater amount of hexavalent chromium, which indicated that the hexavalent chromium in inks derives from the colorants. The soluble hexavalent chromium content in linerboards were below 1 ppm, and no hexavalent chromium was detected in UKP. Results suggested that flexography ink is the main factor to cause hexavalent chromium in linerboard and organic pigments.

The Preparation and the Gas Permeation Characteristics of the Soluble Polyimides (용해성 폴리이미드의 제조 및 기체투과특성)

  • Chun, Kyoung-Yong;Kim, Han-Sung;Han, Hak-Soo;Joe, Yung-il
    • Applied Chemistry for Engineering
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    • v.9 no.2
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    • pp.306-310
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    • 1998
  • The soluble polyimides(PMDA/diamine/dianhydride) were prepared and investigated. After coating the prepared PMDA/diamine/dianhydride solution into the commercial tubular alumina ceramic membranes, gas permeation characteristics was investigated. $T_g{\prime}s$ of the polyimides were in the range of $337{\sim}358^{\circ}C$ and thermal stability was good. The polymer was soluble in NMP, DMAc, DMSO, THF, and m-cresol. The adhesion between the alumina membrane and the soluble polyimide was excellent. The soluble polyimide/alumina membranes containing 6FDA showed the highest permeability among others. The permeability of nitrogen of PMDA/1,3PDA/6FDA-alumina membrane was about $7.6{\times}10^{-7}(mol/m^2{\cdot}Pa{\cdot}s)$ in the gas permeation experiments. The ideal separation factor of $O_2/N_2$ and $H_2/N_2$ in PMDA/1,3PDA/6FDA-alumina membrane were 6.19, and 70.0, respectively.

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Ionic and Elemental Compositions of PM2.5 at the 1,100 m-Highland of Mt. Hallasan in Jeju Island (한라산 1,100 m 지역의 대기 중 PM2.5에 함유된 이온 및 원소 성분의 조성특성)

  • Lee, Ki-Ho;Hu, Chul-Goo
    • Journal of Environmental Science International
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    • v.25 no.6
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    • pp.865-875
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    • 2016
  • In this study, mass concentrations and chemical compositions of $PM_{2.5}$, including water-soluble ions and elements were determined at the 1,100 m-highland of Mt. Hallasan in Jeju Island across four seasons from August 2013 to August 2014. The average mass concentration of $PM_{2.5}$ was $12.5{\pm}8.41{\mu}g/m^3$ with 45.8% of the contribution from eight water-soluble ionic species. Three ionic species ($SO{_4}^{2-}$, $NH{_4}^+$, and $NO{_3}^-$) comprised 96.2% of the total concentration of ions contained in $PM_{2.5}$ and were the dominant ions, accounting for 43.5% of the $PM_{2.5}$ mass at Mt. Hallasan. On the basis of the mass concentration level, seasonal variation, enrichment factor, and relationship among elements, we can presume that Mg, K, Ca, Mn, Fe, Co, Sr, Ba, Nd, and Dy originated mainly from crust or soil and that V, Cr, Ni, Cu, Zn, As, Cd, and Pb were significantly enriched in $PM_{2.5}$ owing to the effects of the anthropogenic emissions. These results and the local distribution of emission sources and topographic characteristics near this sampling site suggest that the compositions of $PM_{2.5}$ collected at the 1100 m-highland of Mt. Hallasan were largely influenced by inflow from outside of Jeju Island.

The High Expressed Serum Soluble Neural Cell Adhesion Molecule, a High Risk Factor Indicating Hepatic Encephalopathy in Hepatocelular Carcinoma Patients

  • Liu, Tian-Hua;Guo, Kun;Liu, Ri-Qiang;Zhang, Shu;Huang, Zhuo-Hui;Liu, Yin-Kun
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.8
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    • pp.3131-3135
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    • 2015
  • Objective: To investigate whether the expression of serum soluble neural cell adhesion molecule (sNCAM) is associated with hepatic encephalopathy (HE) in hepatocelular carcinoma (HCC) patients. Materials and Methods: The Oncomine Cancer Microarray database was used to determine the clinical relevance of NCAM expression in different kinds of human cancers. Sera from 75 HCC cases enrolled in this study were assessed for expression of sNCAM by enzyme linked immunosorbent assay (ELISA). Results: Dependent on the Oncomine Cancer Microarray database analysis, NCAM was down regulated in 10 different kinds of cancer, like bladder cancer, brain and central nervous system cancer, while up-regulated in lung cancer, uterine corpus leiomyoma and sarcoma, compared to normal groups. Puzzlingly, NCAM expression demonstrated no significant difference between normal and HCC groups. However, we found by quantitative ELISA that the level of sNCAM in sera from HCC patients with HE ($347.4{\pm}151.9ng/ml$) was significantly more up-regulated than that in HCC patients without HE ($260.3{\pm}104.2ng/ml$), the p-value being 0.008. sNCAM may be an important risk factor of HE in HCC patients, the correlation coefficients was 0.278 (P<0.05) on rank correlation analysis. Conclusions: This study highlights that up-regulated level of serum sNCAM is associated with HE in HCC patients and suggests that the high expression can be used as an indicator.

Proliferation Effect of Conditioned Medium Produced by Lymph Node Stromal Cells

  • Lee, Sang-Han;Moon, Sung-Hoon;Ha, Sang-Chul;Ma, Jin-Yeul;Kim, Min-Sook;Lee, Jin-Man;Kwon, O-Yu
    • Journal of Life Science
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    • v.12 no.1
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    • pp.22-26
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    • 2002
  • CS21 lymphoma cells that preferentially metastasize to lymph nodes after s.c. inoculation into BALB/c mice were grown in vitro in the presence of CA12 stromal cells isolated from lymph nodes. To obtain fundamental data on identification and characterization of the soluble factor(s) produced by CA12 stromal cells, we investigated the biological profile of the conditioned medium produced by CAl2 stromal cells. CAl2 conditioned medium has no affinity with Con A. CAl2 conditioned medium is associated with the proliferation of splenic T- and thymic T-cells with-out adding mitogen, although the conditioned medium cannot induce the differentiation of thymocytes. Additionally, we showed that H-7, not HA-1004 inhibits CS21 cell proliferation. These results suggest that CAl2 conditioned medium has a specific soluble factor(s) produced by lymph node stromal cells.

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Changes in Free Amino Acids and Sugars in Water-soluble Extracts of Fresh Ginseng during Browning Reaction (수삼물추출물의 갈변반응중 아미노산과 당류변화)

  • 김만욱;박래정
    • Journal of Ginseng Research
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    • v.5 no.2
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    • pp.122-131
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    • 1981
  • An aqueous extract s of fresh ginseng roots was heated at loot for 64 hrs. and the changes of color intensity, pH and the amount of free sugars and amino acids during the various intervals of the heating time were investigated. Color intensity and absorbance of the solution at 490nm were increased in proportion to the length of the heating time. Most of brown pigments produced during the treatment were water soluble, and pH 5.1 at initial stage of the solution, was slightly decreased at the final stages of the reaction. Sucrose, glucose and fructose were major free sugars in ginseng roots, and the amounts of sucrose was over 90 % of total free sugars. Sucrose. was largely decreased approximately 50%, by 64 hrs of the treatment, whereas sharp increase in the amount of glucose and fructose was observed during the reaction in the solution. The observed increase in reducing sugars, glucose and fructose was presumed due to hydrolysis of sucrose. Evidently, glucose and fructose were not important factor to control the browning reaction of the solution. Most of free amino acids and peptides except alanine and isoleucine especially arginine, serine and threonine, were sharply decreased up to 40 : 50% of the original concentration within 2 hrs. Accordingly, the content of free amino acids and peptides seems to be extremely important factor to control the browning reaction in ginseng. A free amino acid, presumed to be nor-leucine, was found in fresh ginseng root on the basis of re mention on liquid chromatography. Kinetic analysis of the browning reaction indicated a pseudo second order with respect to amino acid concentration at the initial stage.

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The Study on the Actual Condition of Customer's Consumption and the Analysis of Market on Food Service Industry Category (외식산업의 유형에 따른 시장분석과 소비 기호성의 탐색적 연구 -스페셜러티(Speciality Coffee) 커피산업을 중심으로-)

  • Gang, Seok-U
    • Journal of Applied Tourism Food and Beverage Management and Research
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    • v.15 no.2
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    • pp.1-22
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    • 2004
  • This study was intended to analysis about quantity and quality aspects of speciality coffee industry in korea. All date was analysed by statistics package program spss 10.0. All market of coffee In korea, the market share of speciailty coffee have been maintained about 10 percent since it was analysed statically about coffee market in korea. The rest market is soluble coffee market. But by comparison with developed country case, korea's coffee market is being unbalanced between soluble coffee and speciality coffee market. This study was analyze actual consumer's consulnption styles of the speciality coffee as well as its industry. According to the qualitative analysis results from peter's model was analysed that made a high growth potentiality and profitability in the future. According to the quantitative analysis results, sampling unit's 87.6 percent made a recognize about the speciality coffee. Sensory evaluation results was researched that factors impacted on the overall speciality coffee quality was shown a significant diffrrence on the two dimension such as the body factor, acidity factor. This study has limit which can not represent overall recognition because of analysis based on limited data. It is thought that steady study should be continued hereafter through more systematic and reliable study method.

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Prion Protein Does Not Interfere with SNARE Complex Formation and Membrane Fusion

  • Yang, Yoo-Soo;Shin, Jae-Il;Shin, Jae-Yoon;Oh, Jung-Mi;Lee, Sang-Ho;Yang, Joo-Sung;Kweon, Dae-Hyuk
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.782-787
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    • 2009
  • In prion disease, spongiform neurodegeneration is preceded by earlier synaptic dysfunction. There is evidence that soluble N-ethylmaleimide sensitive factor attachment receptor (SNARE) complex formation is reduced in scrapie-infected in vivo models, which might explain this synaptic dysfunction because SNARE complex plays a crucial role in neuroexocytosis. In the present study, however, it is shown that prion protein (PrP) does not interfere with SNARE complex formation of 3 SNARE proteins: syntaxin 1a, SNAP-25, and synaptobrevin. Sodium dodecyl sulfate-resistant complex formation, SNAREdriven membrane fusion, and neuroexocytosis of PC12 cells were not altered by PrP. Thus, PrP does not alter synaptic function by directly interfering with SNARE complex formation.