• Title/Summary/Keyword: Solid surface

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Optimization of Medium Composition for Lipopeptide Production from Bacillus subtilis N7 using Response Surface Methodology

  • Luo, Yi;Zhang, Guoyi;Zhu, Zhen;Wang, Xiaohui;Ran, Wei;Shen, Qirong
    • Microbiology and Biotechnology Letters
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    • v.41 no.1
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    • pp.52-59
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    • 2013
  • The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett-Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, $MnSO_4$ and $FeSO_4{\cdot}6H_2O$ were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, $MgSO_4$ 0.37 g/L, $KH_2PO_4$ 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and $NH_4Cl$ (5:3 w/w) 10 g/L, KCl 0.16 g/L, $FeSO_4{\cdot}6H_2O$ 0.24 mg/L, $MnSO_4$ 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production ($706.57{\pm}3.70$ mg/L) in the optimized medium confirmed the validity of the predicted model.

A Study on Molecular Dynamics Method for Improving Characteristics of Ion Implantation (이온주입 특성 개선을 위한 분자동역학적 연구)

  • Yang, Young-Joon;Lee, Chi-Woo
    • Journal of Energy Engineering
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    • v.18 no.2
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    • pp.125-131
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    • 2009
  • Physical characteristics of metals such as hardness, wear-resistance and corrosion-resistance can be artificially controlled by ion implantation. The interaction between ion and solid surface was modeled in molecular scale and simulated by the molecular dynamics method in order to understand the ion implantation mechanism. From the microscopic point of view, the molecular behaviors were observed for improving characteristics of ion implantation. For these purposes, the implantation mechanism and the influences of incident energy, surface temperature and molecular weight were discussed in this study. As the results, the penetration probability was even decreased if incident energy was exceeded any values in the case of high temperature of solid surface. Moreover, it was confirmed that ion implantation into solid surface with amorphous state could be more effective for some conditions.

A Study on the Tribological Characteristics of Low Friction Coating Deposited on SUJ2 Bearing Steel (고탄소크롬 베어링강 2종(SUJ2) 베어링강에 증착된 저마찰 코팅의 트라이볼로지적 특성 연구)

  • Kang, Kyung-Mo;Shin, Dong-Gap;Park, Young-Hun;Kim, Se-Woong;Kim, Dae-Eun
    • Tribology and Lubricants
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    • v.34 no.6
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    • pp.254-261
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    • 2018
  • In order to reduce resistance torque and energy loss, minimizing friction between race surface and rolling elements of a bearing is necessary. Recently, to reduce friction in bearing element, solid lubricant coating for the bearing raceway surface has been receiving much attention. Considering the operating conditions of real bearings, verifying the effect of solid lubricant coatings under extreme conditions of high load that is more than 1 GPa is necessary. In this study, we evaluated the friction and wear characteristics of SUJ2 bearing steels deposited by carbon-based coatings (Si-DLC, ta-C), $MoS_2$ and graphite. In case of $MoS_2$ and graphite coatings, different surface treatments were applied to the coatings to verify the effect of surface treatment. A pin-on-disc type tribotester was used to evaluate the tribological characteristics of the coatings. It was possible to quantitatively estimate the friction and wear characteristics of solid lubricant under dry and lubrication conditions. The carbon-based coatings improved the friction and wear properties of SUJ2 bearing steels under the high load condition, but $MoS_2$ and graphite coatings were not suitable for high load conditions due to its low hardness. Different friction and wear behaviors were found for different substrate surface treatment method. Also, it was confirmed that solid lubricant coatings had a more positive effect than just applying the lubricant for improving the tribological characteristics.

Estimation of Solid Sediments Load by Sewer and Land Surface for Maintenance of Combined Sewer Systems (합류식 관거 유지관리를 위한 하수 및 지표면 고형물 부하량 산정)

  • Lee Jae-Soo;Park Moo-Jong
    • Journal of Korea Water Resources Association
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    • v.39 no.6 s.167
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    • pp.533-544
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    • 2006
  • The deposition of solids in combined sewer systems results in a loss of flow capacity that may restrict flow and cause a local flooding and enhanced solids deposition. In order to solve these problems and proper pipe management, estimation of solid loads from sewer and surface in a drainage basin is needed but this task is very difficult and extremely expensive. In this study, generalized procedures for estimating sewer solid loads during dry weather in combined sewer systems and for estimating solid loads on surface in a drainage basin developed by the U. S. Environmental Protection Agency were applied and analyzed in Gunja drainage basin in Korea. As result, the estimated solid loads from sewer and surface are 205.8,759kg/yr and 1,321,993kg/yr respectively, and total solid loads is 1,527,752kg/yr. The estimated solid removal from street cleaning, dredging from pipe system and pumping house is 1,486,636kg/yr. Therefore, the applied methods show resonable results. More reliable estimation can be achieved if long-term measurements and adjustment of estimation equations are carried out, and this estimation methods can be used usefully for the management of combined sewer system with reduction of cost and effort.

Interplay between Defect Propagation and Surface Hydrogen in Silicon Nanowire Kinking Superstructures

  • Sin, Nae-Cheol
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.221.1-221.1
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    • 2015
  • The vapor-liquid-solid (VLS) method, where the "liquid" catalytic droplets collecting atoms from vapor precursors build the solid crystal layers via supersaturation, is a ubiquitous technique to synthesize 1-dimensional nanoscale materials. However, the lack of fundamental understanding of chemical information governing the process inhibits the rational route to the structural programming. By combining the in situ or operando IR spectroscopy with post-growth high resolution electron microscopy, we show the strong correlation between the surface chemical species concentration and nanowire structures. More specifically, the critical role of surface adsorbed hydrogen, generated from the decomposition of Si2H6 precursor on the interplay between nanowire / kinking and the defect propagation is demonstrated. Our results show that adsorbed hydrogen atoms are responsible for selecting -oriented growth and indicate that a twin boundary imparts structural coherence. The twin boundary, only continuous at / kinks, reduces the symmetry of the trijunction and limits the number of degenerate directions available to the nanowire. These findings constitute a general approach for rationally engineering kinking superstructures and also provide important insight into the role of surface chemical bonding during VLS synthesis.

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Analysis and Measurement of Rough Surface Temperature Rise in Lubricated Condition (거친 표면의 마찰온도 해석 및 온도측정 실험에 관한 연구)

  • Lee, Sang-Don;Cho, Yong-Joo
    • Tribology and Lubricants
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    • v.23 no.2
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    • pp.56-60
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    • 2007
  • The main object of this study is to compare the results that have been concluded by the experiment and to estimate the temperature rise that can cause the contacting surface to be damaged. The former studies are based on the Blok and Jaeger formula. By these formulas we assume that two of the contacted objects are a kind of semi-infinite solid and with this assumption we can make a temperature analysis. But this method doesn't consider lubrication conditions and the calculation time requires a lot of time in that we have to face many difficulties in measuring the actual temperature rise. In this study we combines the semi-infinite solid method and the finite volume method to analyze the temperature of the contacting surface. And we measure temperature rise of the contact surface by dynamic thermocouple.