• 제목/요약/키워드: Solid support

검색결과 356건 처리시간 0.025초

c크기의 폴리스타이렌 담체에 고정화된 단세포군 항체와 항원 BSA의 속도론 (Kinetics of 125I-BSA Binding to Monoclonal Anti-BSA Immobilized on $0.5{\mu}m$ Polystyrene Beads)

  • 박흥우
    • KSBB Journal
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    • 제11권2호
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    • pp.225-237
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    • 1996
  • 고정화된 단세포군 항체와 항원인 알부민과의 결 합시 결합속도기작을 조사하였다. 항체의 고정화에 는 극소의 폴리스타이렌(37^{\circ}C)을 담체로 사용하여 결합속도 측정시 물질저항의 영향을 최소화하였다. 이론적 해석과 실험 결과는 결합반응이 속도론으로 제어됨을 보였는데 결합속도는 2차이고 분리속도는 1차임을 보인다. 고정화 항체와 항원의 평형실험으 로부터의 평형상수는 결합속도상수와 분리속도상수 의 측정치로부터 계산한 값들과 잘 일치한다. 속도 상수들을 $^{\circ}C~37^{\circ}C$에서 측정하였는데 결합속도상 수의 활성화에너지는 9kcal/mole이고 분리속도상수 의 활성화에너지는 2kcal/mole이다. 위의 연구 결과 들은 작은 담체의 사용이 결합속도 기작을 연구하는 데 물질전탈 저항의 영향을 제거할 수 있음을 보이 고 위의 실험 방법들은 고정화 항체의 고유 결합속 도의 측정에 유용할 것이다.

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Production of an Acidic Polygalacturonase from Aspergillus kawachii by Solid State Fermentation and Their Application for Pectin Extraction

  • Martinez-Avila, Guillermo Cristian Guadalupe;Wicker, Louise;Aguilar, Cristobal Noe;Rodriguez-Herrera, Raul;Contreras-Esquivel, Juan Carlos
    • Food Science and Biotechnology
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    • 제18권3호
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    • pp.732-738
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    • 2009
  • An acidic polygalacturonase (PG) from Aspergillus kawachii was produced by solid state fermentation employing a polyurethane foam support. The conditions used for the production of acidic PG were particle size of support (0.6 or 500 $mm^3$) and fermentation time. From the factors studied, the particle size had important influence on enzyme production. The best conditions for acidic PG production were $0.6\;mm^3$ particle size, 18 hr at $30^{\circ}C$ and initial pH of 5.0. In addition, pectin was extracted from citrus pomaces (grapefruit, lime, and tangerine) by acidic PG at $50^{\circ}C$ for 24 hr with citric acid solution. Infrared spectroscopy showed that lime pomace had more high-methoxylated (65%) endogenous pectin than was obtained than from grapefruit or tangerine pomaces. The enzymatically extracted pectin yield in dry basis (d.b.) for grapefruit and lime pectins were 6.95 and 4.25%, respectively. The citric acid solution alone also contributed to pectin extraction from citrus pomaces (7-9%, d.b.). Limited pectin extraction by acidic PG from tangerine pomace was most likely due to the presence of low-methoxylated endogenous pectin. The enzymatic method for pectin extraction using acidic PG from A. kawachii is a promising technique for releasing highly polymerized pectic substances from high-methoxylated lime or grapefruit pomaces.

Decision support Systems research in Korea (1985-1998): An Empirical Investigation through Exploratory Factor Analysis

  • Yoo, Sangjin;Eom, Sean B.
    • 경영과학
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    • 제17권2호
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    • pp.151-159
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    • 2000
  • This study applies factor analysis of an author cocitation frequency matrix derived from a database file that consists of a total of 1095 cited reference records taken from 95 citing decision support systems articles published in Korean journals. This study identified the influence of social psychology, cognitive psychology, management information systems, strategic management, and organizational behavior/organizational design to the development of group support systems, user-interface, and executive information systems research subspecialties. This research establishes a benchmark to be used in the future research, which is necessary to account for the ongoing changes in the intellectual development and structure of DSS Research in Korea before solid conclusion can be reached about the maturity and on-going development of the DSS field in Korean.

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CAPP를 위한 3차원 CAD에서의 공차정보관리에 관한 연구 (A study on 3D CAD tolerance information handling for inspection plnning)

  • 황인식;이관복;하성도
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 1995년도 추계학술대회 논문집
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    • pp.952-956
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    • 1995
  • It is known that the 3D Solid CAD system can provide various information which is useful for implementing CAPP and CAE. However the commercial 3D CAD systems available today do not support the handling of non-geometric information such as geometry tolerance and surface finish. It is impossible to input the non-geometric information during designof parts while CAPP needs the information for selecting machine tools. fiztures, inspection method, etc. In this paper the need of research on handling tolerance information In 3D CAD systems is considered. The development of inspection planning support system is also explained with an example. The development of inspection planning support systm receives the design geometry information from the 3D CAD system in the form of 2D draft and generates the inspection data base and the inspection sheet through the user interaction.

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고속시작 시스템을 위한 삼각형 기반 형상모델링 (Triangle Based Geometric modeling for rapid Prototyping CAM system)

  • 채희창
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 1996년도 춘계학술대회 논문집
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    • pp.587-591
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    • 1996
  • Usually triangular patches are used to transfer geometric shape in Rpaid Prototyping CAM system. STL, a list of triangles, is de facto in RP industry. Because STL has no topology data, it can cause errornous results. So, STL should be verified before using. After adding support structures to anchor the part to the platform and to prevent sagging or distortion, slicing and layer by layer manufacturing process are done. But triangular patch is surface model and cannot provide dufficient information on geometry in the above processes. So, geometric modeling is necessary in verifying STL, adding support structures, and slicing. It is natural that triangle based modeling is the best when traingular patches are used as input. Considering support structures, solid and faces coexist in RP process. Therefore non-manifold modeler is required. In this study, triangle based non-manifold geometric modeling is proposed for RP system consitent with STL input.

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Polymer Support Immobilized Acidic Ionic Liquid: Preparation and Its Application as Catalyst in the Synthesis of Hantzsch 1,4-Dihydropyridines

  • Jahanbin, Bentolhoda;Davoodnia, Abolghasem;Behmadi, Hossein;Tavakoli-Hoseini, Niloofar
    • Bulletin of the Korean Chemical Society
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    • 제33권7호
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    • pp.2140-2144
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    • 2012
  • A polymer support immobilized acidic ionic liquid was prepared by copolymerization of 3-vinyl-1-(4-sulfonic acid)butylimidazolium hydrogen sulfate with styrene in the presence of benzoyl peroxide and its primary application as a solid acidic heterogeneous catalyst to the synthesis of Hantzsch 1,4-dihydropyridines through a one-pot three-component reaction of aromatic aldehydes, ethyl acetoacetate and ammonium acetate was investigated. The results showed that this heterogeneous catalyst has high catalytic activity and the desired products were obtained in good to high yields. Moreover, the catalyst was found to be reusable and a considerable catalytic activity still could be achieved after third run.

STL에 위상정보를 부여하기 위한 삼각형 기반 형상모델링 (Creation of Topological Information from STL Using Triangle Based Geometric Modeling)

  • 채희창
    • 한국정밀공학회지
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    • 제14권2호
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    • pp.136-144
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    • 1997
  • Usually triangular patches are used to transfer geometric shape in Rapid Prototyping CAM system. STL, a list of triangles, is de facto standard in RP industry. Because STL does not have topological infoma- tion, it can cause errornous results. So STL should be verified before using. After adding support structures to anchor the part to the platform and to prevent sagging or distortion, slicing and layer by layer manufactur- ing process are done. But triangular patch is surface model and cannot provide sufficient information on geometry in the above processes. So, geometric modeling is necessary in verifying STL, adding support structures and slicing. It is natural that triangle based modeling is the best when tringular patches are used as input. Considering support structures, solid and faces coexist in RP process. Therefore non-manifold modeler is required. In this study, triangle based non-manifold geometric modeling is proposed for RP sys- tem consistent with STL input.

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고속적층조형법에서 최적 적층방향의 자동결정 (The Automatic Determination of the Optimal Build-Direction in Rapid Prototyping)

  • 채희창
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 1997년도 춘계학술대회 논문집
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    • pp.150-155
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    • 1997
  • Rapid Prototyping(RP) is the technique which is used to make prototypes or functional parts directly using the 3-D solid data. Before building the prototype, several processes such as transfering 3D data from CAD system(STL) determination of build-direction, adding support structure and slicing are required. Among the above processes. determination of build-direction is the target of this study. The build direction is determined by many factors according to the objective of the user, like part accuracy, number of support structure, build time, amount of trapped volume, etc, But it is not easy to determine the build-direction because there are many factors and some factors have dependent properties with one another. So, in this study the part accuracy, the number of support structures and build time are considered as the main factor to determine the optimal build-direction. To determine the optimal build-direction for increasing part accuracy, sum of projected area which caused stairstepping effect was considered. The less the projected area is the better part accuracy is About the optimal build-direction to minimize the amount of support structure, sum of projected area of facets that require support structures was considered. About the build time, we considered the minimum height of part we intended. About the build time, we considered the minimun height of part we intended to make.

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뇌졸중환자의 자기 효능감, 사회적지지, 삶의질 연구 (The Study of Stroke Patient's of Social Support, Self- Efficacy and Qualty of Life)

  • 이혜선
    • 문화기술의 융합
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    • 제4권4호
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    • pp.219-225
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    • 2018
  • 연구의 목적은 뇌졸중환자의 자기효능감과 사회적지지도과 삶의 질을 평가하고 뇌졸중환자의 사회적지지도, 자기 효능감과 삶의 질의 상관성을 알아보고자 하였다. 연구대상은 G시, P시에 소재하는 재활병원에 뇌졸중 진단을 받고 입원해있는 환자 60명을 대상으로 실시하였고 자료 수집은 2017년 8월 1일부터 10 월 1일까지였다. 본 연구 결과를 종합해보면 사회적지지, 자기효능감은 뇌졸중 환자의 삶의 질과 높은 상관관계를 가지고 있는 것으로 판명되었다. 이상의 결과를 토대로 뇌졸중 환자의 삶의 질을 높이기 위한 재활에 있어서 사회적 지지와 자기 효능감 모두 향상시키는 다양한 방법을 치료에 제공한다면 뇌졸중 환자의 삶의 질을 높일 것으로 사료된다.

전착법과 담금법에 의한 음극지지형 SOFC 지르코니아 전해질막 제조 (Preparation of Electrolyte Thin Film for Anode Support Type Solid Oxide Fuel Cells by Electrophoretic Deposition and Dip-Coating)

  • 김상우;이병호;손용배;송휴섭
    • 한국세라믹학회지
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    • 제36권8호
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    • pp.791-798
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    • 1999
  • 다공성 NiO-YSZ 기판위에 전착법(EPD; Electrophoretic Deposition)법과 담금(Dip-coating)법에 의해 음극지지형 고체연료전지용 이트리아 안정화 지르코니아 박막 제조법을 연구하였다. 이를 위해 슬러리 농도 및 시간에 따른 박막의 무게, 박막의 결함 및 미세구조변화에 영향을 주는 제조조건들을 살펴 봄으로써 전착법과 담금법의 차이를 보았다. 담금법에서는 막생성 초기인 30초까지 막의 무게가 증가하지만 그 후에는 탈락이 일어나 시간을 증가하여도 막의 무게가 오히려 감소하였다. 전착법에서는 임계 인가전류 이상에서 시간에 따라 막의 무게가 증가하고 균일하고 치밀한 막이 형성하였다 전장이 매우 낮은 0.035 mA/$cm^2$ 의 정전류를 120초 이상 장시간 인가하면 막의 흘러내림(sagging)으로 인한 결함이 발생하였다. 전착법에 의해 균일하고도 치밀하게 가스 누출성이 없는 음극지지형 고체산화물 연료전지에 적합한 전해질 박막을 제조할 수 있었다.

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