• Title/Summary/Keyword: Solid support

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Kinetics of 125I-BSA Binding to Monoclonal Anti-BSA Immobilized on $0.5{\mu}m$ Polystyrene Beads (c크기의 폴리스타이렌 담체에 고정화된 단세포군 항체와 항원 BSA의 속도론)

  • ;Dan
    • KSBB Journal
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    • v.11 no.2
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    • pp.225-237
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    • 1996
  • Intrinsic binding kinetics of of 125I-bovine serum albumin (125I-BSA) and immobilized monoclonal anti-BSA (MAb 9.1) were studied. Small non-porous polystyrene beads (0.5${\mu}$m diameter) were used as a solid support to minimize the mass transfer interference on rate measurements. We demonstrated both theoretically and experimentally that the binding reaction is kinetically controlled. Rate measurements show that the association reaction is of second order and the dissociation reaction is of first order. Between 4 and $37^{\circ}C$ the measured equilibrium constant agrees well with the equilibrium constant calculated from the rate measurements. The temperature effects on association are much greater than on dissociation; the activation energy for association is about 9Kca1/mole, as compared to 2Kca1/mole for dissociation. The use of small non-porous beads as a solid support in binding studies essentially avoids mass transfer limitations; such a system makes it possible to determine the intrinsic binding characteristics of any immobilized antibody on a solid surface.

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Production of an Acidic Polygalacturonase from Aspergillus kawachii by Solid State Fermentation and Their Application for Pectin Extraction

  • Martinez-Avila, Guillermo Cristian Guadalupe;Wicker, Louise;Aguilar, Cristobal Noe;Rodriguez-Herrera, Raul;Contreras-Esquivel, Juan Carlos
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.732-738
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    • 2009
  • An acidic polygalacturonase (PG) from Aspergillus kawachii was produced by solid state fermentation employing a polyurethane foam support. The conditions used for the production of acidic PG were particle size of support (0.6 or 500 $mm^3$) and fermentation time. From the factors studied, the particle size had important influence on enzyme production. The best conditions for acidic PG production were $0.6\;mm^3$ particle size, 18 hr at $30^{\circ}C$ and initial pH of 5.0. In addition, pectin was extracted from citrus pomaces (grapefruit, lime, and tangerine) by acidic PG at $50^{\circ}C$ for 24 hr with citric acid solution. Infrared spectroscopy showed that lime pomace had more high-methoxylated (65%) endogenous pectin than was obtained than from grapefruit or tangerine pomaces. The enzymatically extracted pectin yield in dry basis (d.b.) for grapefruit and lime pectins were 6.95 and 4.25%, respectively. The citric acid solution alone also contributed to pectin extraction from citrus pomaces (7-9%, d.b.). Limited pectin extraction by acidic PG from tangerine pomace was most likely due to the presence of low-methoxylated endogenous pectin. The enzymatic method for pectin extraction using acidic PG from A. kawachii is a promising technique for releasing highly polymerized pectic substances from high-methoxylated lime or grapefruit pomaces.

Decision support Systems research in Korea (1985-1998): An Empirical Investigation through Exploratory Factor Analysis

  • Yoo, Sangjin;Eom, Sean B.
    • Korean Management Science Review
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    • v.17 no.2
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    • pp.151-159
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    • 2000
  • This study applies factor analysis of an author cocitation frequency matrix derived from a database file that consists of a total of 1095 cited reference records taken from 95 citing decision support systems articles published in Korean journals. This study identified the influence of social psychology, cognitive psychology, management information systems, strategic management, and organizational behavior/organizational design to the development of group support systems, user-interface, and executive information systems research subspecialties. This research establishes a benchmark to be used in the future research, which is necessary to account for the ongoing changes in the intellectual development and structure of DSS Research in Korea before solid conclusion can be reached about the maturity and on-going development of the DSS field in Korean.

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A study on 3D CAD tolerance information handling for inspection plnning (CAPP를 위한 3차원 CAD에서의 공차정보관리에 관한 연구)

  • 황인식;이관복;하성도
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1995.10a
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    • pp.952-956
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    • 1995
  • It is known that the 3D Solid CAD system can provide various information which is useful for implementing CAPP and CAE. However the commercial 3D CAD systems available today do not support the handling of non-geometric information such as geometry tolerance and surface finish. It is impossible to input the non-geometric information during designof parts while CAPP needs the information for selecting machine tools. fiztures, inspection method, etc. In this paper the need of research on handling tolerance information In 3D CAD systems is considered. The development of inspection planning support system is also explained with an example. The development of inspection planning support systm receives the design geometry information from the 3D CAD system in the form of 2D draft and generates the inspection data base and the inspection sheet through the user interaction.

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Triangle Based Geometric modeling for rapid Prototyping CAM system (고속시작 시스템을 위한 삼각형 기반 형상모델링)

  • 채희창
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.587-591
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    • 1996
  • Usually triangular patches are used to transfer geometric shape in Rpaid Prototyping CAM system. STL, a list of triangles, is de facto in RP industry. Because STL has no topology data, it can cause errornous results. So, STL should be verified before using. After adding support structures to anchor the part to the platform and to prevent sagging or distortion, slicing and layer by layer manufacturing process are done. But triangular patch is surface model and cannot provide dufficient information on geometry in the above processes. So, geometric modeling is necessary in verifying STL, adding support structures, and slicing. It is natural that triangle based modeling is the best when traingular patches are used as input. Considering support structures, solid and faces coexist in RP process. Therefore non-manifold modeler is required. In this study, triangle based non-manifold geometric modeling is proposed for RP system consitent with STL input.

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Polymer Support Immobilized Acidic Ionic Liquid: Preparation and Its Application as Catalyst in the Synthesis of Hantzsch 1,4-Dihydropyridines

  • Jahanbin, Bentolhoda;Davoodnia, Abolghasem;Behmadi, Hossein;Tavakoli-Hoseini, Niloofar
    • Bulletin of the Korean Chemical Society
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    • v.33 no.7
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    • pp.2140-2144
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    • 2012
  • A polymer support immobilized acidic ionic liquid was prepared by copolymerization of 3-vinyl-1-(4-sulfonic acid)butylimidazolium hydrogen sulfate with styrene in the presence of benzoyl peroxide and its primary application as a solid acidic heterogeneous catalyst to the synthesis of Hantzsch 1,4-dihydropyridines through a one-pot three-component reaction of aromatic aldehydes, ethyl acetoacetate and ammonium acetate was investigated. The results showed that this heterogeneous catalyst has high catalytic activity and the desired products were obtained in good to high yields. Moreover, the catalyst was found to be reusable and a considerable catalytic activity still could be achieved after third run.

Creation of Topological Information from STL Using Triangle Based Geometric Modeling (STL에 위상정보를 부여하기 위한 삼각형 기반 형상모델링)

  • Chae, Hee-Chang
    • Journal of the Korean Society for Precision Engineering
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    • v.14 no.2
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    • pp.136-144
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    • 1997
  • Usually triangular patches are used to transfer geometric shape in Rapid Prototyping CAM system. STL, a list of triangles, is de facto standard in RP industry. Because STL does not have topological infoma- tion, it can cause errornous results. So STL should be verified before using. After adding support structures to anchor the part to the platform and to prevent sagging or distortion, slicing and layer by layer manufactur- ing process are done. But triangular patch is surface model and cannot provide sufficient information on geometry in the above processes. So, geometric modeling is necessary in verifying STL, adding support structures and slicing. It is natural that triangle based modeling is the best when tringular patches are used as input. Considering support structures, solid and faces coexist in RP process. Therefore non-manifold modeler is required. In this study, triangle based non-manifold geometric modeling is proposed for RP sys- tem consistent with STL input.

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The Automatic Determination of the Optimal Build-Direction in Rapid Prototyping (고속적층조형법에서 최적 적층방향의 자동결정)

  • 채희창
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 1997.04a
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    • pp.150-155
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    • 1997
  • Rapid Prototyping(RP) is the technique which is used to make prototypes or functional parts directly using the 3-D solid data. Before building the prototype, several processes such as transfering 3D data from CAD system(STL) determination of build-direction, adding support structure and slicing are required. Among the above processes. determination of build-direction is the target of this study. The build direction is determined by many factors according to the objective of the user, like part accuracy, number of support structure, build time, amount of trapped volume, etc, But it is not easy to determine the build-direction because there are many factors and some factors have dependent properties with one another. So, in this study the part accuracy, the number of support structures and build time are considered as the main factor to determine the optimal build-direction. To determine the optimal build-direction for increasing part accuracy, sum of projected area which caused stairstepping effect was considered. The less the projected area is the better part accuracy is About the optimal build-direction to minimize the amount of support structure, sum of projected area of facets that require support structures was considered. About the build time, we considered the minimum height of part we intended. About the build time, we considered the minimun height of part we intended to make.

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The Study of Stroke Patient's of Social Support, Self- Efficacy and Qualty of Life (뇌졸중환자의 자기 효능감, 사회적지지, 삶의질 연구)

  • Lee, hye sun
    • The Journal of the Convergence on Culture Technology
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    • v.4 no.4
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    • pp.219-225
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    • 2018
  • This study was conducted to see if a correlation between social support, self-efficacy and quality of life of stroke patients. The study sample was composed of 60 stroke patients who participated in the rehabilitation hospital in G-city and P-city. The survey period for data collection was from August 8.1 - October 10.1, 2017. social support, self-efficacy and quality of life of stroke were administered to those patients. Among the sum of social support, self-efficacy and quality of life marks, a significant correlation was shown statistically at all levels. Through this study, it shows a solid data base is necessary for the improvement of cognitive function in elderly stroke patients.

Preparation of Electrolyte Thin Film for Anode Support Type Solid Oxide Fuel Cells by Electrophoretic Deposition and Dip-Coating (전착법과 담금법에 의한 음극지지형 SOFC 지르코니아 전해질막 제조)

  • 김상우;이병호;손용배;송휴섭
    • Journal of the Korean Ceramic Society
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    • v.36 no.8
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    • pp.791-798
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    • 1999
  • The preparation method of yttria-stabilized zirconia(YSZ) thin film for an anode support type solid oxide fuel cell(SOFC) by electrophoretic deposition(EPD) and dip-coating was studied. And the difference in both preparation method was investigated through basic understanding of processing parameters which may significantly affect weight microstruxcture and defect of film. In dip-coating the thickness of film increased with time until 30 s and then the weight of film decreased with time due to particle falling off from the coagulated film. In EPD although the weight of film increased with time and applied constant-current sagging of the film was observed when the applied current was less that 0.035 mA/$cm^2$ and more than 120 s. Since YSZ thin film by EPD on porous substrate was dense smooth and homogeneous it was expected to be suitable for the electrolyte of an anode support type SOFC.

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