• Title/Summary/Keyword: Solid support

Search Result 356, Processing Time 0.025 seconds

An Antioxidant Capacity Assay Using a Polyvinyl Alcohol-Based DPPH Pellet

  • Ahn, Yeong-Hee;Yoo, Jong-Shin;Kim, Sung-Ho
    • Bulletin of the Korean Chemical Society
    • /
    • v.31 no.9
    • /
    • pp.2557-2560
    • /
    • 2010
  • To assay for antioxidant capacity of natural products considered important in producing human health benefits, a practical and economical method using pellet techniques was developed. A standard visualizing reagent, 1,1diphenyl-2-picryl-hydrazyl (DPPH), was mixed with a water-miscible polyvinyl alcohol (PVA), serving as a solid phase support for the DPPH reagent. A DPPH pellet was prepared by dropping a small volume of the DPPH solution onto PET film, and drying in an oven. The PVA-based DPPH pellet was dissolved into water, in which the water-miscible PVA plays as a non-ionic surfactant to help the DPPH reagent to be dissolved into the solvent. Using the DPPH assay, the antioxidant capacity of water-soluble extracts of black soybean, barley, green tea, and green gram was examined. Among the natural products tested, green tea showed the highest antioxidant capacity. This PVA-based DPPH antioxidant assay can be further applied in the natural food, raw plant material, and health product inspection field.

Dynamic Stability Analysis of Tapered Beck Columns (변단면 Beck 기둥의 동적안정 해석)

  • Lee Byoung-Koo;Lee Tae-Eun;Kang Hee-Jong;Kim Gwon-Sik
    • Proceedings of the Computational Structural Engineering Institute Conference
    • /
    • 2006.04a
    • /
    • pp.949-954
    • /
    • 2006
  • The purpose of this paper is to investigate the stability of tapered columns with clamped one end and carrying a tip mass of rotatory inertia with translational elastic support at the other end. The linearly tapered columns with the solid rectangular cross-section is adopted as the column taper. The differential equation governing free vibrations of such Beck columns is derived using the Bernoulli-Euler beam theory. Both the divergence and flutter critical loads are calculated from the load-frequency curves which are obtained by solving the differential equation. The critical loads are presented as functions of various non-dimensional system parameters: the taper type, the subtangential parameter, mass ratio and spring stiffness.

  • PDF

Cosmetic Industry and Its Development Strategy (화장품산업의 현황 및 발전방안)

  • 김영찬;황순욱;김대중
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.30 no.1
    • /
    • pp.1-6
    • /
    • 2004
  • Although the cosmetic market continuously develops in korea, the foreign product occupies more and more its market3hare in korea. For the future of our industry, institutional support for the ‘functional cosmetics’ (Cosmeceuticals) development should be secured and export infrastructure should be more solid. This paper examines recent trend in cosmetics market (home and foreign) and identifies barriers for the development of cosmetic market. In conclusion, we present several policy strategies for the development of its market.

Nutritional Support for Acute Diarrhea in Children: Focused on Age-appropriate Diet Therapy after Rehydration (영유아 급성 설사의 영양 공급: 탈수 치료 후 연령별 식이요법을 중심으로)

  • Chu, Mi Ae;Choe, Byung-Ho
    • Pediatric Gastroenterology, Hepatology & Nutrition
    • /
    • v.12 no.sup1
    • /
    • pp.53-61
    • /
    • 2009
  • The mainstay in the management of mild to moderately dehydrated children is fast rehydration by using hypotonic ORS (oral rehydration solution) and complete resumption of normal diet, including lactose-containing formula after 4 hours rehydration. Since the majority of young children with uncomplicated acute diarrhea will tolerate large amounts of undiluted non-human milk, withholding food and milk from children during diarrhea is not recommended anymore, regarding time to resolution and diarrhea control. In addition, routine dilution of milk and routine use of lactose-free formula are not necessary after fast ORS therapy. Breastfed infants and children fed with solid foods may safely continue receiving their usual diets during diarrhea instead of gradual reintroduction of feeding. However, young infants or children with severe diarrhea or malnutrition should be carefully treated under supervision if fed with lactose containing, non-human milk exclusively.

Structural Analysis for the Single Crystal of 2-(4-(9H-carbazol-9-yl)benzylidene) based Dye Compound

  • Hwang, Jiyong;Son, Young-A
    • Textile Coloration and Finishing
    • /
    • v.26 no.2
    • /
    • pp.143-149
    • /
    • 2014
  • The designed dye material, namely 2-(4-(9H-carbazol-9-yl)benzylidene) compound, was synthesized. After the reaction, the solid was filtered and purified by recrystalization with acetone/water. To confirm and analyze its synthesis and structural formation, the single crystal was prepared and its measurement was carried out. A yellow needle crystal of $C_{22}H_{13}N_3$ were made on a Rigaku R-AXIS RAPID diffractometer using graphite monochromated CuK${\alpha}$ radiation. All details were suggested and introduced to support and communicate this study.

Microenvironmental Optimizaton of Immobilized Invertase for Methyl- $\beta$ -D-Fructofuranoside Synthesis (Methyl- $\beta$ -D-Fructofuranoside 합성을 위한 고정화 전화당 효소의 미소환경 최적화)

  • 허주형;안형환
    • Journal of the Korea Safety Management & Science
    • /
    • v.1 no.1
    • /
    • pp.259-272
    • /
    • 1999
  • In order to enhance the selectivity, productivity and yield of methyl fructoside, which was synthesized by enzymatic glycosylation of sucrose and methanol solution, controlling of surface property of solid support using different immobilization procedures optimized microenvironment of immobilized invertase. Silanization and polyethylene imine coating methods were adopted to give a hydrophobic and hydrophilic environment of immobilized invertase. As a result, polyethyleneimine coating method gave higher loading of enzyme, effective activity, and relative activity than silanization method, because it brought on increasing the functional density of amino group and enhancing the conservation of activity by regulating of hydrophilicity. And then, hydrophilic environment was possible to restraint the assessing of methyl fructoside molecule, which was more hydrophobic than sucrose, fructose, and glucose molecule in the reaction mixture, into .the active site of immobilizedinvertase. Consequently, hydrophilic microenvironment of immobilized invertase by polyethyleneimine coating obtained higher yield and productivity with increasing conversion than silanized and native invertase. Thus, this procedure optimized the microenvironment of immobilized invertase suitable for the enzymatic synthesis of methyl fructoside.

  • PDF

Hardware accelerated Voxelization using a Stencil Buffer (Stencil Buffer를 이용한 형상의 복셀화)

  • Jang Dong Go;Kim Gwang Su
    • Proceedings of the Korean Operations and Management Science Society Conference
    • /
    • 2002.05a
    • /
    • pp.266-271
    • /
    • 2002
  • We propose a hardware accelerated voxelization method for various 3D object model such as surface models, solid models, and volumetric CSG models. The algorithm utilizes the stencil buffer that is one of modern Open히 graphics hardware features. The stencil buffer is originally used to restrict drawing to certain portions of the screen. The volumetric representations of given 3D objects are constructed slice-by-slice. For each slice, the algorithm restricts the drawing areas constructed inner region of 3D objects using the stencil buffer, and generates slices of the volumetric representation for target objects. As a result, we can provide volume graphics support for various engineering applications such as multi-axis machining simulation, collision detection and finite element analysis.

  • PDF

Phase Equilibria and Reaction Paths in the System Si3N4-SiC-TiCxN1-x-C-N

  • H.J.Seifert
    • Journal of Powder Materials
    • /
    • v.6 no.1
    • /
    • pp.18-35
    • /
    • 1999
  • Phase equilibria in the system Si3N4-TiC-TiCxN1-x-C-N were determined by thermodynamic calculations (CALPHAD-method). The reaction peaction paths for Si3N4-TiC and SiC-TiC composites in the Ti-Si-C-n system were simulated at I bar N2-pressure and varying terpreatures. At a temperature of 1923 K two tie-triangles (TiC0.34N0.66+SiC+C and TiC0.13N0.87+SiC+Si3N4) and two 2-phase fieds (TiCxN1-x+SiC; 0.13

$NH_2-terminal$ Amino acid Sequence Analysis of Monoclonal Antibody by Electroblotting Method (Electroblotting을 이용한 단일클론항체의 $NH_2$-말단 아미노산 배열분석)

  • Nam, Kyung-Soo;Chang, Hyeun-Wook;Chung, Kyu-Charn
    • YAKHAK HOEJI
    • /
    • v.34 no.1
    • /
    • pp.11-14
    • /
    • 1990
  • $NH_2-terminal$ amino acid sequence analysis of monoclonal antibody is very important to identify gene family and diversities of antigen-antibody recognition. When we used the PVDF (Polyvinylidene difluoride) membrane blotting method, we could easily analyze $NH_2-terminal$ sequence of monoclonal-antibody which specifically binds to phosphatidylinositol 4,5-biphosphate. PVDF membrane is an ideal solid-phase support for sequence analysis, especially when used with electroblotting method. This method is superior to continual method and will be applied to the sequence analysis of picomole quantities of proteins by gel electrophoresis.

  • PDF

Eco-friendly Solventless Synthesis of 5-Indolylpyrimido[4,5-d]pyrimidinones and Their Antimicrobial Activity

  • Gupta, Ragini;Jain, Anshu;Joshi, Rahul;Jain, Meenakshi
    • Bulletin of the Korean Chemical Society
    • /
    • v.32 no.3
    • /
    • pp.899-904
    • /
    • 2011
  • A series of 5-indolylpyrimido[4,5-d]pyrimidinones (4a-h) were obtained by multi-component reaction of 3-formylindole (1), thiobarbituric acid/barbituric acid (2) and thiourea/urea (3) under microwave irradiation in dry media. Secondly, grinding together neat reactants also gave the titled compounds in good yields. All the synthesized compounds have been characterized on the basis of elemental analyses and spectral data (IR, $^1H$ NMR and Mass). Representative compounds were also evaluated for their antimicrobial activity against Rhizopus stolonifer, Fusarium oxysporum, Escherichia coli, and Pseudomonas aeruginosa at different concentrations. Some of the compounds showed promising activity.