Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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An Antioxidant Capacity Assay Using a Polyvinyl Alcohol-Based DPPH Pellet

  • Ahn, Yeong-Hee (Mass Spectrometry Division, Korea Basic Science Institute) ;
  • Yoo, Jong-Shin (Mass Spectrometry Division, Korea Basic Science Institute) ;
  • Kim, Sung-Ho (Department of Chemistry, Soonchunhyang University)
  • Received : 2010.04.17
  • Accepted : 2010.07.26
  • Published : 2010.09.20
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Abstract

To assay for antioxidant capacity of natural products considered important in producing human health benefits, a practical and economical method using pellet techniques was developed. A standard visualizing reagent, 1,1diphenyl-2-picryl-hydrazyl (DPPH), was mixed with a water-miscible polyvinyl alcohol (PVA), serving as a solid phase support for the DPPH reagent. A DPPH pellet was prepared by dropping a small volume of the DPPH solution onto PET film, and drying in an oven. The PVA-based DPPH pellet was dissolved into water, in which the water-miscible PVA plays as a non-ionic surfactant to help the DPPH reagent to be dissolved into the solvent. Using the DPPH assay, the antioxidant capacity of water-soluble extracts of black soybean, barley, green tea, and green gram was examined. Among the natural products tested, green tea showed the highest antioxidant capacity. This PVA-based DPPH antioxidant assay can be further applied in the natural food, raw plant material, and health product inspection field.

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References

  1. Boo, Y. C.; Jeon, C. O.; Kim, J. S.; Park, S. N. Preprint, 1st Scientific Conference of the Asian Soc. Cosm. Sci. 1993, 143-149.
  2. Lloyd, B. J.; Siebenmorgen, T. J.; Beers, K. W. Cereal Chem. 2000, 77, 551-555. https://doi.org/10.1094/CCHEM.2000.77.5.551
  3. Benzie, I. F.; Strain, J. J. Anal. Biochem. 1996, 239, 70-76. https://doi.org/10.1006/abio.1996.0292
  4. Koutelidakis, A. E.; Argiri, K.; Serafini, M.; Proestos, C.; Komaitis, M.; Pecorari, M.; Kapsokefalou, M. Nutrition 2009, 25, 453-458. https://doi.org/10.1016/j.nut.2008.10.007
  5. Dudonne, S.; Vitrac, X.; Coutiere, P.; Woillez, M.; Mérillon, J. M. J. Agric. Food Chem. 2009, 57, 1768-1774. https://doi.org/10.1021/jf803011r
  6. Ghiselli, A.; Serafini, M.; Natella, F.; Scaccini, C. Free Radic. Biol. Med. 2000, 29, 1106-1114. https://doi.org/10.1016/S0891-5849(00)00394-4
  7. Prior, R. L.; Hoang, H.; Gu, L.; Wu, X.; Bacchiocca, M.; Howard, L.; Hampsch-Woodill, M.; Huang, D.; Ou, B.; Jacob, R. J. Agric. Food Chem. 2003, 51, 3273-3279. https://doi.org/10.1021/jf0262256
  8. Alma, M. H.; Mavi, A.; Yildirim, A.; Digrak, M.; Hirata, T. Biological and Pharmaceutical Bulletin 2003, 26, 1725-1729. https://doi.org/10.1248/bpb.26.1725
  9. Kordali, S.; Cakir, A.; Mavi, A.; Kilic, H.; Yildirim, A. J. Agric. Food Chem. 2005, 53, 1408-1416. https://doi.org/10.1021/jf048429n
  10. Bandoniene, D.; Murkvic, M. J. Agric. Food Chem. 2002, 50, 2482-2487. https://doi.org/10.1021/jf011475s
  11. Cheng, Z.; Jeffrey, M.; Yu, L. J. Agric. Food Chem. 2006, 54, 7429-7436. https://doi.org/10.1021/jf0611668
  12. Chyu, K. Y.; Babbidge, S. M.; Zhao, X.; Dandillaya, R.; Rietveld, A. G.; Yano, J.; Dimayuga, P.; Cercek, B. Circulation 2004, 109, 2448-2453. https://doi.org/10.1161/01.CIR.0000128034.70732.C2
  13. Mittal, A.; Pate, M. S.; Wylie, R. C.; Tollefsbol, T. O.; Katiyar, S. K. Int. J. Oncol. 2004, 24, 703-710.
  14. Katiyar, S.; Elmets, C. A.; Katiyar, S. K. J. Nutr. Biochem. 2007, 18, 287-296. https://doi.org/10.1016/j.jnutbio.2006.08.004

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