• 제목/요약/키워드: Sole

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Three Separate Pathways for the Initial Oxidation of Limonene, Biphenyl, and Phenol by Rhodococcus sp. Strain T104

  • Kim, Dockyu;Park, Min-Jung;Koh, Sung-Cheol;So, Jae-Seong;Kim, Eungbin
    • Journal of Microbiology
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    • 제40권1호
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    • pp.86-89
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    • 2002
  • Rhodococcus sp. strain T104, which is able to grow on either biphenyl or limonene, was found to utilize phenol as sole carbon and energy sources. Furthermore, T104 was positively identified to possess three separate pathways for the degradation of limonene, phenol, and biphenyl. The fact that biphenyl and limonene induced almost the same amount of catechol 1,2-dioxygenase activity indicates that limonene can induce both upper and lower pathways for biphenyl degradation by T104.

Thiobacillus concretivorus의 분리 및 동정에 관한 연구 (Studies on isolation and identification of thiobacillus concretivorus)

  • 하영칠;차승희;안승구
    • 미생물학회지
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    • 제10권4호
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    • pp.155-166
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    • 1972
  • With respect to a bacterium isolated some of the similarities and differences previously indicated between Thiobacillus thiooxidans and Thiobacillus concretivorus wee confirmed and the bacteria also provided considerable diferences compared to the two species in some repects. Severe precipitation of sulfur occurred in colonies and in liquid media of the organism isolated. The organism isolated utilized nitrate and asparagine as well as ammonium sulfate as a sole nitrogen source and grew well without being nearly inhibited by citrate and malate. This organism also showed the resistance to heats and external physical stimulations. Owing to some characters decribed above and the reports proposed, the organism isolated could be concluded to be Thiobacillus concretivorus and it was suggested that Thiobacillus concretivorus might be an apparently different species from Thiobacillus thiooxidans.

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토양으로부터 Malonate를 이용하는 Acinetobacter calcoaceticus의 분리 (Isolation of a Malonate-utilixing Acinetobacter calcoaceticus from Soil)

  • 김성준;김유삼
    • 미생물학회지
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    • 제23권3호
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    • pp.230-234
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    • 1985
  • Malonate를 유일한 탄소원으로 활용할 수 있는 세균을 토양으로부터 분리하였다. 이 세균은 행태, 배양, 생리 그리고 생화학적 연구를 통하여 Acinetobacter calcoaceticus임이 확인되었다. 이 미생물을 malonate를 유일한 탄소원으로 하는 배지에서 배양하였을 갱우, malonyl CoA synthetase, isocitrate lyase 및 malate, synthase가 유도 되었다. 따라서 이 미생물에서도 Pseudomonas fluorescens에서 제안되었던 대사경로 즉 $malonate{\rightarrow}malonyl-CoA{\rightarrow}acetyl-CoA{\rightarrow}glyoxylate\;cycle$을 통하여 malonate를 이용하는 것으로 판단된다.

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.betha.-1, 3-glucanase 생성균의 분리 및 효소 생성 조건 (Isolation of .betha.-1, 3-glucanase producing strain and cultural conditions of its enzyme production)

  • 정기택;방광웅;송형익;김재근;유대식
    • 미생물학회지
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    • 제24권3호
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    • pp.295-301
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    • 1986
  • The bacteria, which were capable of producing ${\beta}-1$, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination. The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. the enzyme production was reached maximum state shen the broth cultured for 72hr at $30^{\circ}C$. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% $MnSO_4$ at pH 7.5.

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Catabolism of 4-Hydroxybenzoic Acid by Pseudomonas sp. DJ-12

  • Tim;Chae, Jong-Chan;Kim, Chi-Kyung
    • Journal of Microbiology
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    • 제37권3호
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    • pp.123-127
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    • 1999
  • A Pseudomonas sp. strain DJ-12 isolated by 4-cholrobiphenyl enrichment culture technique is capable of utilizing 4-hydroxybenzoic acid as a sole source of carbon and energy. The bacterium catabolized 4-hydroxybenzoic acid through the intermediate formation of protocatechuic acid, which was further metabolized. The cell free extracts of pseudomonas sp. DJ-12, grown on 4-hydroxybenzoic acid showed higher activities of 4-hydroxyenzoate 3-hydroxylase and protocatechuate 4,5-dioxygenase, but the activity of catechnol 2,3-dioxygenase was lower. The results suggest that 4-hydroxybenzoic acid is catabolized via protocatechuic acid rather than catechol or gentisic acid in this bacterium and that the protocatechuic acid formed was metabolized through a metacleavage pathway by protocatechuate 4,5-dioxygenase.

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Synthesis of Poly(1,6-heptadiyne) Derivatives Containing Bulky Substitutents by Metathesis Polymerization

  • Jin, Sung-Ho;Jin, Jeong-Yi;Kim, Young-Inn;Park, Dong-Kyu;Gal, Yeong-Soon
    • Macromolecular Research
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    • 제11권6호
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    • pp.501-505
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    • 2003
  • Poly(l,6-heptadiyne) derivatives with phenoxy and 3,7-dimethyloctyloxyphenoxy substituents were synthesized using metathesis polymerization. Polymerizations using MoCl$\sub$5/ as the sole catalyst gave high yields and the resulting polymers were completely soluble in organic solvents. The polymers' structures and thermal properties were characterized using NMR and UV-Vis spectroscopy, as well as with TGA and DSC thermograms. From the analysis of the high-resolution $\^$13/C-NMR spectra, we was found that these polymers contain six-membered rings. The number-average molecular weights and polydispersities of the polymers were ca. 7.0∼20 ${\times}$ 10$^3$ and 3.1∼5.8, respectively. The glass transition temperatures of the polymers were found to be in the range 91∼159$^{\circ}C$.

Abscisic Acid Binding to Extracts from Normal and Viviparous-1 Mutant Aleurone Layers of Zea mays L.

  • Bai, Dong-Gyu
    • Journal of Plant Biology
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    • 제37권2호
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    • pp.151-158
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    • 1994
  • Aleurone layers of normal and vp1 mutant maize kernels were extracted and centrifuged at 100,000g to yield a cytosol fraction. Binding of [3H]ABA cis, trans (+)ABA to a soluble macromolecular components present in the cytosol was demonstrated by Sephadex chromatography and non-denaturing PAGE. The binding component was of high molecular weight and seems to be an aggregate of proteins. A rapid DEAE-cellulose filter method for assaying bound [3H]ABA to a soluble protein was adapted. Binding assays were performed with cytosol that had been preheated or incubated with several enzymes, indicating that heat and protease treatments disrupted the binding. This suggested that binding occurred to proteins. Some properties of the ABA binding proteins were described. The [3H]ABA binding were reduced dramatically when unlabeled ABA was added as a competitor, suggesting a specific binding of [3H]ABA. Gel filtration profiles and autoradiogram of [3H]ABA binding showed no difference in the binding components of Vp1 and vp1/vp1 mutant cytosol, indicating that Vp1 protein is not a sole ABA binding protein.

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식품조사(食品照射) 실용화(實用化)에 대한 배경(背景)과 전망(展望) (Background and Prospect for the Commercialization of Food Irradiation)

  • 권중호
    • 한국식품영양과학회지
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    • 제14권1호
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    • pp.88-94
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    • 1985
  • Food irradiation is increasingly recognized as the sole viable alternative to traditional method of food preservation in terms of reducing the overall quantity of spoiled food, reducing energy used in food storage, and reducing reliance on chemicals known to be hazardous but currently required for disinfestation. Irradiation with ionizing radiation can be applied in the fields of sprout inhibition, disinfestation of insects, sterilization, delay of ripening, and improvement of organoleptic properties in food. In order to back up the commercialization of food irradiation in Korea, this review not only dealt with the international background for food irradiation and wholesomeness of irradiated food, but also evaluated economic feasibility of irradiated food, irradiation facilities, and domestic status of food irradiation studies.

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에지 추출 향상을 위한 Mean Shift 알고리즘의 이용 (Using Mean Shift Algorithm Enhance Edge Detection Effect)

  • ;신성윤;이양원
    • 한국컴퓨터정보학회:학술대회논문집
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    • 한국컴퓨터정보학회 2008년도 제39차 동계학술발표논문집 16권2호
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    • pp.211-214
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    • 2009
  • Edge detection always influenced by noise belong to the original image, therefore need use some methods to sort this issue, mean shift algorithm has the smooth function which suit for the edge detection purpose, so adopted to fade out the unimportant information, and the sensitive noise portions. After this section, use the Canny algorithm to pick up the contour of the objects we focus on, meanwhile select the Soble operator that has the orientation attribute to support the method work well. In final, take experiment and get the perfect result we wanted, make sure this method make sense and better than the sole Edge detection algorithm,

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Adjusted Techniques by Architects Trained as Craftsmen - Cases of Adolf Loos, Mies van der Rohe, Peter Zumthor -

  • Song, Ha-Yub
    • Architectural research
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    • 제12권1호
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    • pp.33-38
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    • 2010
  • Many theorists acknowledge that Modern architecture denied the architect's crafty gesture for the sake of pure formal and compositional status of a building. As well, modern technology in construction has been prevailing as the sole support for modern architecture. But there exists architects who proposed a dialectic development of traditional and modern techniques. This thought was prominent in Germanic circle where technological development was in advance. Throughout $20^{th}$ century, the works of Adolf Loos, Mies Van der Rohe, and Peter Zumthor are representatives in each period. A common point begins from their apprenticeships as craftsmen: Loos and Mies as stonemasons, Zumthor as a cabinetmaker. More than this fact, their craftsmanship is embedded in their works and express creativity of architects. While mass production system raises non-participatory practice that merely require assemblage of products, the adjusted techniques with craftsmanship brings forth a participatory practice that does not limit the creativity of architects.