• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.13-18
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• 2003

This study was carried out to investigate the expression patterns of foreign gene that controlled by tuber-specific patatin promoter in transgenic potatoes. Potato leaf disc cultured in vitro were transformed by the Agrobacterium strain LBA4404 containing pBl121 or pATGUS from potato cv. Desiree. In order to select the transgenic lines, gene-specific primers deduced from the NPTII were synthesized and used for polymerase chain reaction. The down part of the putative transgenic potatoes was transplanted weekly onto sucrose-enriched medium to accelerate the microtuber formation. RNA gel blot analysis was performed on the total RNAs obtained from tuber that had been harvested at a week interval. Also, histochemical assay was observed in the explants transformed with either pBI121 or pATGUS. Results showed that the transgenic plant containing pATGUS expressed GUS transcripts mainly at the tuber, not in stem, with the highest expression level in 5 weeks-grown microtubers. In contrast to pATGUS plants, the transformed plants with pBI121 showed an equal expression pattern throughout the whole developing stages. Consistent with RNA gel blot analysis, histochemical GUS staining and enzyme activity exhibited pATGUS transcripts were at the highest level in 5 weeks cultures. From these results, we suggest that the best stage to analyze the foreign gene introduced by patatin promoter into potato plants is at 5 weeks cultures after tuber formation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.251-255
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• 2008

This study was carried out in 2002 to determine the usefulness of Jeju scoria for a component of a growth medium and optimum planting density of 'Dejima' seed potatoes (Solanum tuberosum L.) grown in a wick hydroponic system. The minitubers ($7.0{\pm}0.2\;g$) produced in an aeroponics system were planted at five planting densities (3 to 15 tubers/box; 19 to $95\;tubers/m^2$) in polystyrene boxes (51 cm long $\times$ 31 cm wide $\times$ 20 cm high) containing two media (perlite + peatmoss and Jeju scoria + peatmoss 1:2, v/v mixtures). There were no significant interactions between medium and planting density for the growth and tuber yield traits. Shoot growth and the number of tubers per plant were not significantly affected by the media. However, tuber yield was higher in the perlite + peatmoss mixture than in the Jeju scoria + peatmoss mixture. The percentage of underdeveloped plants ranged from 8.3 to 14.7% at four lower planting densities (3 to 12 tubers/ box), and was 25.8% at the highest planting density (15 tubers/box). As planting density was increased from 3 to 15 tubers per box, seed potato (${\geq}5\;g$ tuber) number increased from 101 to 269 and yield from 6.3 to $11.6\;kg/m^2$. These results indicate that the perlite + peatmoss mixture might be more suitable for seed potato production in the wick hydroponic system, and considering the percentage of underdeveloped plants and tuber yield, optimum planting density would be 56 to $76\;tubers/m^2$ in the system depending on availability of seed potatoes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.164-173
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• 2018

Elevated atmospheric carbon dioxide concentration ($CO_2$) is a major component of climate change, and this increase can be expected to continue into the crop and food security in the future. In this study, Soil-Plant-Atmosphere-Research (SPAR) chambers were used to examine the effect of elevated $CO_2$, temperature, and drought on the canopy architecture and concentration of macronutrients in potatoes (Solanum tuberosum L.). Drought stress treatments were imposed on potato plants 40 days after emergence. Under AT+2.8C700 (30-year average temperature + $2.8^{\circ}C$ at $700{\mu}mol\;mol^{-1}$ of $CO_2$), at maximum leaf area, elevated $CO_2$, and no drought stress, a significant increase was observed in both the aboveground biomass and tuber, and for the developmental stage. Even though $CO_2$ and temperature had increased, AT+2.8C700DS (30-year average temperature + $2.8^{\circ}C$ at $700{\mu}mol\;mol^{-1}$ of $CO_2$ under drought stress) under drought stress showed that the leaf area index (LAI) and dry weight were reduced by drought stress. At maturity, potatoes grown under $CO_2$ enrichment and no drought stress exhibited significantly lower concentrations of N and P in their leaves, and of N, P, and K in tubers under AT+2.8C700. In contrast, elevated $CO_2$ and drought stress tended to increase the tuber Mg concentration under AT+2.8C700DS. Plants grown in AT+2.8C700 had lower protein contents than plants grown under ATC450 (30-year average temperature at $400{\mu}mol\;mol^{-1}$ of $CO_2$). However, plants grown under AT+2.8C700 showed higher tuber bulking than those grown under AT+2.8C700DS. These findings suggest that the increase in $CO_2$ concentrations and drought events in the future are likely to decrease the macronutrients and protein concentrations in potatoes, which are important for the human diet.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1130-1136
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• 2011

This study was carried out to determine the effects of pig slurry on growth of potato (Solanum tuberosum L. cv. Dejima), soil chemistry properties and infiltration water quality in volcanic ash soil and non-volcanic ash soil of Jeju. Fertilization of liquid pig manure was based on nitrogen. In volcanic ash soil and non-volcanic ash soil, there was no difference in the height and diameter of stems in chemical fertilizer and liquid pig manure application treatments. Also yields of potatoes were no significantly difference in chemical fertilizer and liquid pig manure application treatments. pH in all soil was increased by application of liquid pig manure compared to the chemical fertilizer plot. Contents of exchangeable K in all soil were accumulated excessively by fertilization of pig manure 100% compared to the chemical fertilizer 100%. But there was no difference between the chemical fertilizer 50%+liquid pig manure 50% and chemical fertilizer 100%. No difference between the chemical fertilizer and liquid pig manure was observed in available phosphate, exchangeable Ca and Mg. $NO_3$-N concentration of infiltration water sample collected at 70cm of soil depth was lower non-fertilizer than chemical fertilizer and liquid pig manure application treatments. In volcanic ash soil, the $NO_3$-N concentration of infiltration water was decreased from early, except liquid manure 100%. In non volcanic ash soil, the $NO_3$-N concentration of infiltration water increased until October 8, but then was reduced. In all soils, $NO_3$-N concentration of infiltration water was higher in the liquid manure 100% than those in the chemical fertilizer 100% and chemical fertilizer 50%+liquid pig manure 50%, but there were no differences. In conclusion, the growth of potato, fertilization of soil and $NO_3$-N content of infiltration water were not different between chemical 50%+liquid pig manure 50% and chemical 100% plot. So, liquid pig manure could be substituted for some amount of chemical fertilizer.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.3
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• pp.145-154
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• 2022

Due to abnormal weather conditions caused by climate change, natural disasters and damages are gradually increasing around the world. Global climate change as accompanied by warming is projected to exert adverse impact on production of potato, which is known as cool season crop. Even though, role of potato as a food security crop is expected to increase in the future, the climate change impacts on potato and adaption strategies are not sufficiently established. Therefore, this study was conducted to analyze the damage pattern of potatoes due to high temperature treatment and to evaluate the response of cultivars. T he high temperature treatment (35~38℃) induced heat stress by sealing the plastic house in midsummer (July), and the quantity and quality characteristics of potatoes were compared with the control group. T otal yield, marketable yield (>80 g) and the number of tubers per plants decreased when heat treatment was performed, and statistical significance was evident. In the heat treatment, 'Jayoung' cultivar suffered a high heat damage with an 84% reduction in yield of >80 g compared to the control group. However, in Jopung cultivar, the decrease was relatively small at 26%. Tuber physiological disturbances (Secondary growth, Tuber cracking, Malformation) tended to increase in the heat stress. Under heat conditions, the tubers were elongated overall, which means that the marketability of potatoes was lowered. T he tuber firmness and dry matter content tended to decrease significantly in the heat-treated group. T herefore, the yield and quality of tubers were damaged when growing potatoes in heat conditions. T he cultivar with high heat adaptability was 'Jopung'. T his result can be used as basic data for potato growers and breeding of heat-resistant cultivars.