• 식물병연구
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• 제21권3호
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• pp.180-185
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• 2015

In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato ${\beta}$-tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.

• The Plant Pathology Journal
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• 제29권3호
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• pp.285-293
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• 2013

Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ~1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ~580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.

• Molecules and Cells
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• 제22권1호
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• pp.89-96
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• 2006

"Determinate" and "indeterminate" inflorescences in plants are controlled by a single recessive gene, for example, SELF-PRUNING (SP) in Solanum lycopersicum, TERMINAL FLOWER1 in Arabidopsis, CENTRORADIALIS in Antirrhinum, and CENTRORADIALIS-like gene in tobacco. Pepper (Capsicum annuum L.) is an indeterminate species in which shoots grow indefinitely. In this study, we cloned and characterized the pepper SP-like gene (CaSP). RT-PCR revealed that the CaSP transcript accumulates to higher levels in floral buds than in other organs. Comparison of genomic DNA and cDNA sequences from indeterminate and determinate pepper plants revealed the insertion of a single base in the first exon of CaSP in the determinate pepper plants. CaSP is annotated in linkage group 8 (chromosome 6) of the SNU2 pepper genetic map and showed similar synteny to SP in tomato. Transgenic tobacco plants overexpressing CaSP displayed late-flowering phenotypes similar to the phenotypes caused by overexpression of CaSP orthologs in other plants. Collectively, these results suggest that pepper CaSP is an ortholog of SP in tomato.

• Molecules and Cells
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• 제37권1호
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• pp.36-42
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• 2014

The tomato (Solanum lycopersicum L.) is a model plant for genome research in Solanaceae, as well as for studying crop breeding. Genome-wide single nucleotide polymorphisms (SNPs) are a valuable resource in genetic research and breeding. However, to do discovery of genome-wide SNPs, most methods require expensive high-depth sequencing. Here, we describe a method for SNP calling using a modified version of SAMtools that improved its sensitivity. We analyzed 90 Gb of raw sequence data from next-generation sequencing of two resequencing and seven transcriptome data sets from several tomato accessions. Our study identified 4,812,432 non-redundant SNPs. Moreover, the workflow of SNP calling was improved by aligning the reference genome with its own raw data. Using this approach, 131,785 SNPs were discovered from transcriptome data of seven accessions. In addition, 4,680,647 SNPs were identified from the genome of S. pimpinellifolium, which are 60 times more than 71,637 of the PI212816 transcriptome. SNP distribution was compared between the whole genome and transcriptome of S. pimpinellifolium. Moreover, we surveyed the location of SNPs within genic and intergenic regions. Our results indicated that the sufficient genome-wide SNP markers and very sensitive SNP calling method allow for application of marker assisted breeding and genome-wide association studies.

• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.787-804
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• 2018

Flavor quality is import for determining consumer perception and acceptance of tomato products. In this study, 'Fendou' tomato fruit were harvested at six ripening stages and sampled to investigate the development of flavor-relevant compounds during vine ripening. Results showed that upon the initiation of ripening there was an increase in respiration rate and concomitant ethylene evolution that was associated with increased membrane permeability. In accordance with these physiological changes, flavor-relevant compounds demonstrated different expression patterns as fruit ripened, which contributed to 'red-ripe' flavor characteristics of red-ripe fruit. Based on correlation analysis between ethylene evolution and the flavor-relevant compounds during 'Fendou' tomato ripening and the other researchers' reports, the activation of System 2-dependent autocatalytic ethylene production plays an important role in the development of most flavor-relevant compounds during tomato vine ripening. Overall, our results suggested that most flavor-relevant compounds that accumulated the most during tomato fruit ripening at red stage could be under ethylene regulation and were among the most important contributors to the 'red-ripe' flavor. Due to the development of these compounds, the flavor quality at late ripening stages is different from that of fruit at early ripening stages.

• 생물환경조절학회지
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• 제18권4호
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• pp.413-419
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• 2009

토마토 펄라이트 자루재배에서의 효율적인 점적핀설치 위치를 규명하기 위해서, 지제부로부터 5cm 위치에 고정(F5), 15cm 고정(F15) 및 5cm~15cm로 이동(M5-15)의 3처리구를 두었다. 점적 핀 위치에 따른 배지 내 뿌리 분포를 알아보기 위해서 자루 배지를 9등분해 염색한 결과, F5 처리구에서는 다른 처리에 비해 뿌리량이 지제부 가까이에 좀 더 많았고 지제부에서 멀어질수록 뿌리량은 적었다. 지제부에 가까울수록 뿌리는 하위로 많이 발달해 있으며, 멀수록 배지 전체에 균일하게 분포하는 경향을 보였다. F15 처리구에서는 지제부에서 멀수록 뿌리가 많이 밀집되어 있었고 지제부에서 멀어질수록 위쪽에 많이 분포되어 있었다. 점적 핀 위치가 지제부로부터 5cm 위치에서 15cm로 이동한 M5-15 처리구에서는 전체적으로 뿌리가 균일하게 분포되어 있었다. 펄라이트 자루 내 배지 부위별 수분 분포를 생육단계에 따라 조사한 결과, 처리에 관계없이 수직분포는 하부로 갈수록 수분함량이 많았으며, 하부에서 특히 많았다. 관수종료 1시간 후에는 상부에서는 차이가 없었지만, 중부 및 하부에서는 지제부에 가까울수록 수분함량이 많았다. 중앙과 양측 면 사이에 큰 차이는 없었다. 수확량은 F15 처리구에서 가장 많은 경향을 나타내었다. 이상의 결과, 펄라이트 자루재배에서는 점적핀을 지제부로부터 15cm에 위치시키는 것이 뿌리분포나 수확량에 가장 좋은 것으로 나타났다.

• 생물환경조절학회지
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• 제18권4호
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• pp.408-412
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• 2009

펄라이트 자루재배시 일중 관수마감시각을 달리함으로써 최적 생장을 유지하면서도 용수이용효율(WUE)과 비료이용효율(FUE)을 높이기 위해 실험을 실시하였다. 관수마감시각에 따라 일몰 1시간 전부터 4시간 전까지 4단계로 나누어 처리한 결과, 배지 내 수분함량은 마감시각이 늦을수록 많은 경향을 나타냈다. 최종 생육은 일몰 4시간 전 처리구에서 약간 낮은 경향을 보이기는 했으나, 통계적 유의차는 보이지 않았다. 상품수량은 일몰 1시간 전 및 2시간 전에서 가장 많았고, 4시간전에서 가장 적었다. 관수마감시각 처리별 128일 동안의 급액량을 조사한 결과, WUE와 FUE는 일몰마감 1시간 전 처리구에서 가장 낮았고, 3시간 전 처리구에서 가장 높았다. 식물생육, 수확량 및 WUE와 FUE 등의 면에서 일몰 4시간 전에 관수를 중단하는 것은 바람직하지 않으며, 2~3시간 전에 마감하는 것이 좋을 것으로 사료된다.

• The Plant Pathology Journal
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• 제33권2호
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• pp.163-169
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• 2017

$SIMAPKKK{\alpha}$, a tomato (Solanum lycopersicum) mitogen-activated protein kinase kinase kinase, is a positive regulator of Pto-mediated effector-triggered immunity, which elicits programmed cell death (PCD) in plants. In this study, we examined whether putative phosphorylation sites in the conserved activation segment of the $SIMAPKKK{\alpha}$ kinase domain are critical for eliciting PCD. Three amino acids, $threonine^{353}$, $serine^{360}$ ($Ser^{360}$), or $serine^{364}$ ($Ser^{364}$), in the conserved activation segment of $SIMAPKKK{\alpha}$ kinase domain were substituted to alanine (T353A, S360A, or S364A), and these variants were transiently expressed in tomato and Nicotiana benthamiana plants. Two alanine substitutions, S360A and S364A, completely abolished $SIMAPKKK{\alpha}$ PCD-eliciting activity in both plants, while T353A substitution did not affect its PCD-eliciting activity. $SIMAPKKK{\alpha}$ wild type and variant proteins accumulated to similar levels in plant leaves. However, $SIMAPKKK{\alpha}$ protein with the largest size was missed when either S360A or S364A substitutions were expressed, whereas proteins with the smaller masses were more accumulated than those of full-length of $SIMAPKKK{\alpha}$ and T353A. These results suggest that phosphorylation of $SIMAPKKK{\alpha}$ at $Ser^{360}$ and $Ser^{364}$ is critical for PCD elicitation in plants.

• 한국토양비료학회지
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• 제45권6호
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• pp.910-919
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• 2012

The role of inorganic nitrogen assimilation in the production of amino acids, organic acids and soluble sugars is one of the most important biochemical processes in plants, and, in order to achieve normally, nitrate uptake and assimilation is essential. For this reason, the characterization of nitrate assimilation and metabolite composition from leaves, roots and xylem sap of tomato (Solanum lycopersicum) was investigated under different nitrate levels in media. Tomato plants were grown hydroponically in liquid culture under five different nitrate regimes: deficient (0.25 and 0.75 mM $NO_3{^-}$), normal (2.5 mM $NO_3{^-}$) and excessive (5.0 and 10.0 mM $NO_3{^-}$). All samples, leaves, roots and xylem sap, were collected after 7 and 14 days after treatment. The levels of amino acids, soluble sugars and organic acids were significantly decreased by N-deficiency whereas, interestingly, they remained higher in xylem sap as compared with N-normal and -surplus. The N-excessive condition did not exert any significant changes in metabolites composition, and thus their levels were similar with N-normal. The gene expression and enzyme activity of nitrate reductase (NR), nitrite reductase (NIR) and glutamine synthetase (GS) were greatly influenced by nitrate. The data presented here suggest that metabolites, as a signal messenger, existed in xylem sap seem to play a crucial role to acquire nitrate, and, in addition, an increase in ${\alpha}$-ketoglutarate pathway-derived amino acids under N-deficiency may help to better understand plant C/N metabolism.

• 원예과학기술지
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• 제29권6호
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• pp.568-574
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• 2011

토마토 펄라이트 베드재배에서 급액방법으로 배액전극 제어법을 적용하기 위한 적정 측정틀 개발을 위해 본 실험이 수행되었다. 작물로는 대과종 토마토를 사용하였고, 재배 방법은 자루재배와 동일하게 적용하였으며, 급액제어 방법으로 배액전극 제어법을 적용한 다섯 가지 측정틀 처리와 타이머 제어법을 적용한 한 가지 측정틀 처리를 대조구로 하였다. 1차 실험에서는 배지의 수분함량 안정성 조사로 가장 불안정한 Tube-2 처리를 제외하였고, 2차 실험에서는 배지의 수분함량 안정성과 생육과 수확량을 조사하여 성적이 가장 나빴던 Tube-1 처리를 제외하였다. 3차 실험에서 배지의 수분함량 안정성과 생육 및 수확량에서 우수하였으며, WUE와 FUE가 가장 높았던 Up-Board 처리를 선택하였다. Up-Board 처리는 여섯 가지 처리 중에서 가장 경제적이며 측정틀 제작도 용이하여 베드충진 방식에서 배액전극 제어법을 적용하기에 가장 우수한 측정틀 설계였다.