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Molecular Cloning of a Pepper Gene that Is Homologous to SELF-PRUNING  

Kim, Dong Hwan (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Han, Myeong Suk (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Cho, Hyun Wooh (Department of Plant Sciences, College of Agriculture and Life Sciences, Seoul National University)
Jo, Yeong Deuk (Department of Plant Sciences, College of Agriculture and Life Sciences, Seoul National University)
Cho, Myeong Cheoul (National Horticultural Research Institute, Rural Development Administration)
Kim, Byung-Dong (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Publication Information
Molecules and Cells / v.22, no.1, 2006 , pp. 89-96 More about this Journal
Abstract
"Determinate" and "indeterminate" inflorescences in plants are controlled by a single recessive gene, for example, SELF-PRUNING (SP) in Solanum lycopersicum, TERMINAL FLOWER1 in Arabidopsis, CENTRORADIALIS in Antirrhinum, and CENTRORADIALIS-like gene in tobacco. Pepper (Capsicum annuum L.) is an indeterminate species in which shoots grow indefinitely. In this study, we cloned and characterized the pepper SP-like gene (CaSP). RT-PCR revealed that the CaSP transcript accumulates to higher levels in floral buds than in other organs. Comparison of genomic DNA and cDNA sequences from indeterminate and determinate pepper plants revealed the insertion of a single base in the first exon of CaSP in the determinate pepper plants. CaSP is annotated in linkage group 8 (chromosome 6) of the SNU2 pepper genetic map and showed similar synteny to SP in tomato. Transgenic tobacco plants overexpressing CaSP displayed late-flowering phenotypes similar to the phenotypes caused by overexpression of CaSP orthologs in other plants. Collectively, these results suggest that pepper CaSP is an ortholog of SP in tomato.
Keywords
Capsicum annuum L.; Determinate Inflorescence; Linkage Map; Phylogenic Tree; SELF-PRUNING (SP);
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1 Amaya, I., Ratcliffe, O. J., and Bradley, D. J. (1999) Expression of CENTRORADIALIS (CEN) and CEN-like genes in tobacco reveals a conserved mechanism controlling phase change in diverse species. Plant Cell 11, 1405−1418   DOI   ScienceOn
2 Bradley, D., Carpenter, R., Copsey, L., Vincent, C., Rothstein, S., et al. (1996a) Control of inflorescence architecture in Antirrinum. Nature 379, 791−797
3 Gustafson-Brown, C., Savidge, B., and Yanofsky, M. F. (1994) Regulation of the Arabidopsis floral homeotic gene APETALA1. Cell 14, 131−143
4 Ratcliffe, O. J., Amaya, I., Vincent, C. A., Rothstein, S., Carpenter, R., et al. (1998) A common mechanism controls the life cycle and architecture of plants. Development 125, 1609− 1615
5 Ratcliffe, O. J., Bradley, D. J., and Coen, E. S. (1999) Separation of shoot and floral identity in Arabidopsis. Development 126, 1109−1120
6 Lander, E., Green, P., Abrahamson, J., Barlow, A., Daley, M., et al. (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174−181
7 Ahn, J. H., Miller, D., Winter, V. J., Banfield, M. J., Lee, J. H., et al. (2006) A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J. 25, 605− 614   DOI   ScienceOn
8 Mandel, M. A., Gustafson-Brown, C., Savidge, B., and Yanofsky, M. F. (1992) Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature 260, 273−277   DOI
9 Kang, B. C., Nahm, S. H., Huh, J. H., Yoo, H. S., Yu, J. W., et al. (2001) An interspecific (Capsicum annuum x C. chinense) F2 linkage map in pepper using RFLP and AFLP markers. Theor. Appl. Genet. 102, 531−539
10 Felsenstein, J. (1993) PHYLIP (Phylogeny Inference Package) version 3.5c. distributed by the author. Department of Genetics, University of Washington
11 Alvarez, J., Guli, C. L., Yu, X.-H., and Smyth, D. R. (1992) TERMINAL FLOWER. A gene affecting inflorescence development in Arabidopsis thaliana. Plant J. 2, 103−116   DOI
12 Mimida, N., Sakamoto, W., Murata, M., and Motoyoshi, F. (1999) TERMINAL FLOWER 1-like genes in Brassica species. Plant Sci. 142, 155−162   DOI   ScienceOn
13 Yeung, K., Seitz, T., Li, S., Janosch, P., McFerran, B., et al. (1999) Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401, 173−177   DOI   ScienceOn
14 Pnueli, L., Carmel-Gore, L., Hareven, D., Gutfinger, T., Alvarez, J., et al. (1998) The SELF-PRUNING gene of tomato regulates vegetative to reproductive switching of sympodial meristems and is the ortholog of CEN and TFL1. Development 125, 1979−1989
15 Weigel, D., Alvarez, J., Smyth, D. R., Yanofsky, M. F., and Meyerowitz, E. M. (1992) LEAFY controls floral meristem identity in Arabidopsis. Cell 69, 843−860
16 Ronen, G., Carmel-Goren, L., Zamir, D., and Hirschberg, J. (2000) An alternative pathway to $\beta$-carotene formation in plant chromoplasts discovered by map-based cloning of Beta and old-gold color mutations in tomato. Proc. Natl. Acad. Sci. USA 97, 11102−11107
17 Chung, E. S., Seong, E. S., Kim, Y. C., Chung, E. J., Oh, S. K., et al. (2004) A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang). Mol. Cells 17, 377−380
18 Lee, J. M., Nahm, S. H., Kim, Y. M., and Kim, B. D. (2004) Characterization and molecular genetic mapping of microsatellite loci in pepper. Theor. Appl. Genet. 108, 619−627   DOI   ScienceOn
19 Bowman, J. L., Alvarez, J., Weigel, D., Meyerowitz, E. M., and Smyth, D. R. (1993) Control of flower development in Arabidopsis thaliana by APETAIA1 and interacting genes. Development 119, 721−743
20 Jensen, C. S., Salchert, K., and Nielsen, K. K. (2001) A TERMINAL FLOWER1-like gene from perennial ryegrass involved in floral transition and axillary meristem identity. Plant Physiol. 125, 1517−1528   DOI   ScienceOn
21 Shannon, S. and Meeks-Wagner, D. R. (1991) A mutation in the Arabidopsis TFL1 gene affects inflorescence meristem development. Plant Cell 3, 877−892   DOI   ScienceOn
22 Nahm, S. H., Yu, J. W., Kang, B. C., and Kim, B. D. (1997) Election of parental lines of hot pepper mapping population using RFLP and AFLP analysis. J. Kor. Soc. Hort. Sci. 38, 693−697
23 Lee, M. H. (1994) Genetic analysis of determinate plant shape, fruit bearing habit and deciduous character of hot pepper (Capsicum annuum L.). Ph.D. dissertation. Chungbuk National University
24 Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions- specific gap penalities and weight matrix choice. Nucleic Acids Res. 22, 4673−4680   DOI   ScienceOn
25 Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., et al. (2006) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 31, 3497−3500   DOI   ScienceOn
26 Pillitteri, L. J., Lovatt, C. J., and Walling, L. L. (2004) Isolation and characterization of a TERMINAL FLOWER homolog and its correlation with juvenility in Citrus. Plant Physiol. 135, 1540−1551   DOI   ScienceOn
27 Pnueli, L., Gutfinger, T., Hareven, D., Ben-Naim, O., Ron, N., et al. (2001) Tomato SP-interacting proteins define a conserved signaling system that regulates shoot architecture and flowering. Plant Cell 13, 2687−2702   DOI   ScienceOn