Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Molecular Cloning of a Pepper Gene that Is Homologous to SELF-PRUNING

  • Kim, Dong Hwan (Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Han, Myeong Suk (Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Cho, Hyun Wooh (Department of Plant Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Jo, Yeong Deuk (Department of Plant Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Cho, Myeong Cheoul (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Byung-Dong (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
  • Received : 2006.04.22
  • Accepted : 2006.06.11
  • Published : 2006.08.31
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Abstract

"Determinate" and "indeterminate" inflorescences in plants are controlled by a single recessive gene, for example, SELF-PRUNING (SP) in Solanum lycopersicum, TERMINAL FLOWER1 in Arabidopsis, CENTRORADIALIS in Antirrhinum, and CENTRORADIALIS-like gene in tobacco. Pepper (Capsicum annuum L.) is an indeterminate species in which shoots grow indefinitely. In this study, we cloned and characterized the pepper SP-like gene (CaSP). RT-PCR revealed that the CaSP transcript accumulates to higher levels in floral buds than in other organs. Comparison of genomic DNA and cDNA sequences from indeterminate and determinate pepper plants revealed the insertion of a single base in the first exon of CaSP in the determinate pepper plants. CaSP is annotated in linkage group 8 (chromosome 6) of the SNU2 pepper genetic map and showed similar synteny to SP in tomato. Transgenic tobacco plants overexpressing CaSP displayed late-flowering phenotypes similar to the phenotypes caused by overexpression of CaSP orthologs in other plants. Collectively, these results suggest that pepper CaSP is an ortholog of SP in tomato.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation (KOSEF)

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