• Title/Summary/Keyword: Solanum

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Effect of IOA and Media on Production of Somatic Hybrid by the Asymmetric Protoplast Fusion Between Three Solanum Species

  • Kim Hag-Hyun;Kwon Soo-Jeong;Shin Un-Dong
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.418-421
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    • 2006
  • In this study, we surveyed the influence of IOA (iodoacetamide) and media upon protoplast fusion for the efficient production of the somatic hybrid among S. sisymbriifolium and other Solanum species (S. intergrifolium and S. toxicarium). Regardless of a breed, as the IOA concentration increases, the cell division tends to decrease. As the influence of the media on the colony formation, we could get 5 colonies from the fusion of S. sisymbriifolium and IOA-treated S. integrifolium protoplast, but none was observed in other fusions. As a result of analyzing their IDH isozyme, we found a somatic hybrid in 2 objects.

Anticancer Properties of Total Alkaloid fraction of Solanum pseudocapsicum unripe fruits

  • Badami, Shrishailappa;Dhonde, Satish G.;Reddy SA, Manohar;P., Vijayan;B, Suresh
    • Advances in Traditional Medicine
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    • v.4 no.1
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    • pp.49-52
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    • 2004
  • The total alkaloid fraction of the methanolic extract of Solanum pseudocapsicum unripe fruits was tested for its in-vivo anticancer activity against Dalton's Lymphoma Ascites model in mice. The total alkaloid fraction at 5.0 and 10.0 mg/kg body weight showed significant increase in the mean survival time and the percentage increase in the life span of tumor bearing mice. The increase in the body weight was found to be less than that of the control. However, the treatment at 20 mg/ kg body weight was found to be toxic and showed a decrease in the mean survival time, and body weight when compared to control mice. The antitumor activity observed may be due to the cytotoxic activity of the total alkaloid fraction.

Antioxidant Activity of Ethanolic Extract of Potato(Solanum tuberlosum) (감자(Solanum tuberlosum) 에탄올 추출물의 황산화 활성)

  • Choi Eun-Mi;Koo Sung-Ja
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.5
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    • pp.479-486
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    • 2004
  • 감자는 전세계적으로 섭취되는 주요 작물로서 우리나라에서는 감자의 생즙을 관절염 및 통증을 억제하는 민간요법으로 사용되어 왔다. 본 연구에서는 감자 에탄을 추출물의 항산화 작용을 검토하였다. 감자 에탄올 추출물(0.05~5 mg/mL) 은 1,1-diphenyl-2-picrylhydrazyl radical에 대하여 강한 free radical scavenger 활성을 나타내었다. 또한 감자 추출물(0.5 mg/mL)은 쥐간 균질액 에서 생성 된 TBARS의 양을 유의적으로 억제하였으며 (p<0.05) Fe/sup 2+//ascorbate induction system에서 감자 추출물(0.5~2 mg/mL)은 DNPH 반응으로 측정된 carbonyl 형성을 억제하였다. 항산화 활성과 마찬가지로 감자 추출물의 환원력은 0.5 mg/mL 농도에서 가장 높았고 이는 α-tocopherol의 경우와 비슷하였다. 이 결과는 감자 추출물이 항산화력을 통해 생체에서 작용할 수 있는 가능성을 제시한다.

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Studies on The Anther Culture of Solanum nigrum L. II (Solanum nigrum L.의 약배양에 관한 연구 II)

  • 한창열
    • Journal of Plant Biology
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    • v.14 no.2
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    • pp.7-10
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    • 1971
  • Haploid cell obta-ined from microspores of Solanum nigrum were cultured on two kinds of medium, "Callus-inducing medium" and "Differentiation medium", in order to conduct histological studies of callus and examine differentiation of plantlets. On the callus-inducing medium the calli grew rapidly. The bulk of callus mass was light brown colored "Wet callus" covered on the surface with thin layers of rough and gleaming "White callus". The wet callus was consisted of parenchyma and meristematic tissues, while the white callus had no meristematic tissues. Large parenchyma cells, by successive divisions, became multicellular or poly nucleate cells which developed later to be meristematic tissues. The calli embedded on the differentiation medium quickly turned to dark brown color. Plantlets, however, came out later from these blackened callus mass. In the callus sectioned about ten weeks after imbedding on the differentiation medium, radially elongated tissue, concentric tissue, epidermis, tracheid-like structure, and plant jprimordia were observed.ure, and plant jprimordia were observed.

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Radio-protective Effects of the Extracts of Various Medicinal Plants against Human Normal Lung Cells

  • Lee, Ke-Yong;Kim, Chang-Han;Cho, Choa-Hyung
    • Natural Product Sciences
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    • v.8 no.4
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    • pp.144-146
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    • 2002
  • Rhapontica uniflora, Platycodon grandiflorum and Solanum tuberosum extracts affected the recovery of radiation-induced damage at all tested doses $(15.6,\;31.3,\;62.5\;and\;125\;{\mu}g/ml)$. The survival rate of pretreatment with extracts was increased by 2 times more than its compared with untreated cells. All tested extracts protected the growh-delay of normal cells and haematological parameters from their radiation-induced fall. In tested extracts, Solanum tuberosum was significantly superior to Platycodon grandiflorum and Rhapontica uniflora in radio-protection activity.

Studies on Growth Characteristics and Yield of Solanum njgrum L. (까마중(Solanum nigrum L.)의 생육특성과 전초수량에 관한 연구)

  • Choi, Seongkyu;Seo, Kyoungsun
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.596-602
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    • 2012
  • This study was carried out to effect on Growth Characteristics and Yield of Solanum njgrum L. The experimental results are summarized as follows. Germination, growth characteristics and yield of Solanum nigrum Linne collected from southern parts of Korea were investigated. The one fruit weight was the heaviest in Gochang traditional S. nigrum among the collected sites. The number of seeds in one fruit was high in the orders of Gochang traditional sites, Gwangju traditional sites, Suncheon traditional sites and Naju traditional sites. The earliest flowering of Solanum nigrum was observed on 10. August. The plant height, stem diameter, leaf numbers and branch numbers were ranged from 65 cm to 75 cm, 7.1 mm to 8.2 mm, 237 ea to 270 ea and 12 ea to 17 ea, respectively. The fresh weight per plants were ranged from 265 g to 279 g, and yield of biomass were ranged from 1,298 kg to 1,367 kg per 10a. The insect pest numbers of S. nigrum according to the collected sites were showed no significant in this test.

Culture and Fusion of Protoplasts from Potato (Solanum tuberosum L.) and Tobacco (Nicotiana tabacum L.) (감자(Solanum tuberosum L.)와 담배 (Nicotiana tabacum L.)의 원형질체 배양 및 융합)

  • 정상호
    • Journal of Plant Biology
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    • v.30 no.4
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    • pp.287-298
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    • 1987
  • The regenerative capacities of protoplasts isolated from potato (Solamum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

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Effect of Solution Culture System on Growth and Mini-tuber Yield of Hydroponically Grown Potato(Solanum tuberosum L. cv. Dejima) (양액재배방식이 '대지' 감자(Solanum tuberosum L.)의 생육 형 소괴경 형성에 미치는 영향)

  • 김기택;박용봉
    • Journal of Bio-Environment Control
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    • v.6 no.3
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    • pp.198-204
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    • 1997
  • This study was conducted to investigate the effect of solution culture systems on growth and mini-tuber yields of hydroponlcally grown potato (Solanum tuberosun L. cv. Dejima). The growth of stem and foliage at 40 and 60 days after planting were better in aeroponics system than the other systems, and stem length and number of stems per plant at 90 days after planting were the greatest as of 65.4 cm, 7.3 in aeroponics and the lowest as of 49.5 cm, 3.2 in scoria media system. Stolon length was the longest as of 30.4 cm in aeroponics at 90 days after planting, and number of stolons was the greatest as of 10.5 in NFT. Number of mini-tubers per plant at 90 days after planting was 67.1, 62. 5, 20.1 and 18.0 in aeroponics, NFT, perlite and scoria media, respectively. The perlite media system made the fewest enlargement of lenticels of mini-tubers. The results indicate that aeroponic system can be used effectively for mini-tuber potato production.

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Isolation and Characterization of a cDNA Encoding CycD3 Gene from Potato(Solanum tuberosum L.) (감자 (Solanum tuberosum L.) CycD3유전자의 분리 및 특성 분석)

  • Kang, In-Hong;Choi, Seung-Ho;Lee, Hong-Geun;Hwang, Hyun-Sik;Lee, Suk-Chan;Jung, Tae-Young;Lim, Hak-Tae;Bae, Shin-Chul
    • Journal of Plant Biotechnology
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    • v.30 no.4
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    • pp.329-334
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    • 2003
  • D-type cyclins are believed to regulate the G1 to S phase transition in response to nutrient and hormonal signals. We investigated the expression characteristics of the key cell-cycle regulators, mitotic and G1 cyclins in potato (Solanum tuberosum L.). We isolated D-type cyclin gene from potato and it was classified as D3 cyclin by sequence similarities and a phylogenetic analysis, and named as StcycD3;1. The accumulation of transcripts was predominantly associated with mitotically active organs, such as stolons, roots, flowers, leaves, and stems. Transcription of StcycD3;1 can be induced by sucrose.