• Title/Summary/Keyword: Solanum

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Anti-hypertensive Activity of New Potato(Solanum tuberosum) Variety of Gui Valley Via Inhibition of Angiotensin Converting Enzyme (안지오텐신 전환효소활성 저해에 의해 항고혈압 효과를 갖는 신품종 감자 구이벨리 추출물)

  • Kim, Kwan-Hyun;Kim, Sun-Hee;Lee, Eun-Ok;Kwon, Hyun-Jung;Choi, Jong-Won;Lim, Hak-Tae;Kim, Sung-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.1
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    • pp.93-96
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    • 2009
  • This study was attempted to investigate the anti-hypertensive activity of Solanum tuberosum, cv. Gui valley. Ethanol extract of Gui vally (EGV) increased free radical scarvenge activity up to 91.6% of control at $200{\mu}g/m{\ell}$. It's anti-oxidant activity is similar to 10 uM of ascorbic acid, well known as antioxidant. EGV inhibited Angiotensin-I-converting enzyme (ACE) activity in vitro. ACE plays a important role in regulation of blood pressure and ACE inhibitors are important for the treatment of hypertension. Anti-hypertensive activity of EGV in vivo was assessed in lead acetate (LAT)-induced hypertensive rats for 8 weeks. Elevated blood pressure in control group was significantly decreased by EGV at 200 mg/kg. Also, ACE activity in blood was also suppressed by EGV treatment. Taken together, these results suggest that EGV has an anti-hypersive activity via inhibition of ACE and can be used for the treatment or prevention of hypertension.

Cloning and Characterization of GDP-mannose Pyrophosphorylase from Solanum Tuberosum L.

  • Hyun, Tae-Kyung;Lim, Jung-Dae;Kim, Jae-Kwang;Seong, Eun-Soo;Lee, Jae-Geun;Yoon, Byeong-Sung;Kim, Myong-Jo;Cho, Dong-Ha;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.5
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    • pp.276-283
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    • 2005
  • Ascorbic acid is a great antioxidant and helps protect the body against pollutants. GDP-mannose pyrophosphorylase (GMPase) is a key enzyme in manufacturing GDP-mannose, a glycosyl donor for ascorbate and cell wall biosynthesis as well as for protein glycosylation. In this study, we described molecular cloning of a full-length cDNA from Potato (Solanum tuberosum L. cv. Jasim), using tuber. The cDNA isolated encoded a GDP-mannose pyrophosphrylase. The nucleotide sequence of pGMPC showed about 95%, 89% and 80% homology with S. tuberosum (AF022716), N. tabacum (AB066279) and A. thaliana (AF076484) cDNAs clone known as GMPase, respectively. We detected the expression of GMPase using RT-PCR. The highest expression of GMPase was found in stems, and the largest amount of ascorbic acid was also presented in stems. In contrast, the leaf showed minimal level of GMPase transcript and ascorbic acid content. We propose that GMPase expression patterns were similar to the changes of ascorbic acid content in the leaves treated with diverse stresses.

Antioxidant Activities of Extracts of Solanum nigrum L. (용규 추출물의 항산화 활성 탐색)

  • Kim, A-Ram;Kim, Dong-Hyun;Pyeun, Soo Min;Jeong, Gangwon;Gwon, Jae Hwan;Yeo, Chung Won;Lee, Jung-Seag;Jeong, Gwi-Taek
    • KSBB Journal
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    • v.29 no.6
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    • pp.421-425
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    • 2014
  • In this study, the antioxidant activities of fractions (methanol, hexane, methylene chloride, butanol, ethyl acetate and water) of Solanum nigrum L. extract was investigated. The contents of total phenolic compounds of each fractions of methanol, butanol, methylene chloride, ethyl acetate, hexane and water are $4.41{\pm}0.23%$, $5.57{\pm}0.35%$, $9.89{\pm}0.19%$, $9.86{\pm}0.19%$, $1.89{\pm}0.04%$, and $3.18{\pm}0.06%$, respectively. For assay of antioxidant activity, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity, reducing power and nitrite scavenging activity are evaluated. In DPPH radical scavenging activity, the highest effect was obtained from the fraction of ethyl acetate. Reducing power is ordered as ethyl acetate > methylene chloride > methanol. In nitrite scavenging activity, the highest activity was 5.5% (butanol fraction), whereas hexane fraction did not detected. Overall, antioxidant activities are closely related the content of phenolic compound in extracts of S. nigrum L.

Introduction of Maize Transposable Elements, Ac and Ds into the Genome of a Diploid Potato Species (옥수수 전위유전자 Ac 및 Ds의 2배체종 감자 Genome 내로의 도입)

  • 김화영;임용표
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.39-45
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    • 2000
  • Two maize transposable elements, immobilized Ac (iAc) and Ds, have been introduced into the genome of a diploid potato clone (Solanum tuberosum Group Phureja clone 1.22). The iAc is a modified Ac that is supposed to be unable to transpose but is expected to trans-activate the transposition of a Ds that is unable to transpose by itself. When the leaf and stem explants of in vitro shoots of the clone 1.22 were inoculated with Agrobacterium tumefaciens strains harboring binary vectors containing the iAc and the Ds, calli were formed from the explants on media containing 50 mg/L of kanamycin, and shoots were regenerated from the calli. The regenerated shoots formed roots when cultured on media containing 100 mg/L of kanamycin, whereas untransformed shoots did not form roots on the same media. The PCR amplification of the DNA's from the transgenic plants confirmed that the iAc and the Ds elements were introduced into the potato genome of 1.22.

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Effects of Planting Density and Fertilizer Level on Agronomic Characteristics and Yield of Solanum njgrum L. (재식밀도와 시비량이 야생약초 까마중(Solanum njgrum L.)의 주요 형질과 수량에 미치는 영향)

  • 최성규;이종일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.4
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    • pp.340-344
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    • 1991
  • This study was investigated to find out the optimum planting densities fo Solanum njrum L. about growth characters. yield components and yield at 6 planting densities by direct sowing culture. The results obtained are summarized as follows: Studying the correlationship. the highly negative significances were found between planting densities and fresh eights weights per plant, on the other hand, the highly positive significances were found between planting densities and plant height. Plant height was long in dense planting, and short in spacious planting by direct sowing culture. Stem diameter was thick in spacious planting. and was thin in dense planting. Fresh wt. per plant was decreased in dense planting and was increased in spacious planting by direct sowing culture. Yield was highest in dense planting densities (10 x 20cm : 50plants/$m^2$) by direct sowing culture. Fertilizing ((N: P$_2$O$_{5}$ $K_2$O=6 : 6 : 6Kg/10a) was increased than without fertilizing because plant height and number of branch was good.d.

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Study on Antioxidative Activity of Plant Extracts in Fish Oil (식물 추출물이 어유의 산화 안정성에 미치는 영향)

  • Jang, Ji-Sun;Hong, Jang-Hwan;Lee, Ki-Teak
    • Food Science and Preservation
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    • v.13 no.6
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    • pp.726-731
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    • 2006
  • Extract from forty four species of natural plants in Korea were obtained. Each extinct (1,000 ppm) was added into fish oil and their antioxidative activities were screened by rancimat, From the results, seven species including Brassica juncea, Solanum tuberosum, Capsella bursa, Ulums davidiana, Broussonetia kazinoki, Oenanthe javanica, and Rhus chinensis were selected due to their relatively prolonged induction periods. When the concentration (100 - 1,000 ppm) of the selected extract was considered, the most prolonged induction period was observed from Solanum tuberosum throughout the studied concentrations, suggesting that its extinct showed the strongest antioxidative activity against fish oil. In addition, peroxide value (POV) and thiobarbituric acid reactive substance (TBARS) value were considered at $60^{\circ}C$ for 5 days. Among the selected extract, extract from Solanum tuberosum showed the lout POV while the extract from Brassica juncea var showed the least TBARS values after 5 days. Also, the free radical scavenging effect against DPPH radical were studied with the selected extract, showing that the extract from Rhus chinensis was found to be the most effective.

Biological Control Effects of Hedera helix, Lantana camara, Solanum pseudocapsicum (아이비, 란타나, 예루살렘체리 추출물의 생물적 방제 효과)

  • Lee, Kyu Hoi;Kim, Ju Hyoung;Kim, Hyun Ju;Choi, Won Ho;Jang, Tae-Won;Choi, Ji-Soo;Park, Min-Jeong;Mun, Jeong-Yun;Im, Jong-Yun;Kim, Do-Wan;Park, Jae-Ho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.76-76
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    • 2018
  • 화학적 살충제는 빠르고 정확하게 해충을 방제하는 효과를 나타내지만, 인간과 다른 유기체에 대한 살충제 잔류의 위험성, 화학적 살충에 대한 저항성을 가진 해충의 출현, 생태계의 교란 등 다양한 부작용이 있다. 이 때문에 생물적 방제를 통한 해충 방제는 효과적인 수단이 될 수 있다. 특히 채소 작물 등에 주로 발생하는 응애는 생산량 저하 및 품질 저하 등과 같은 다양한 문제를 일으킬 수 있다. 특히 진딧물이나 점박이응애는 화학적 살충제에 대해 높은 저항성이 발달되어 문제가 되고 있다. 본 연구에서는 아이비(Hedera helix), 란타나(Lantana camara), 예루살렘체리(Solanum pseudocapsicum)를 통한 천연 식물 추출물의 응애에 대한 생물적 방제 효과를 확인하였다. 각 추출물은 0.5%, 0.05% 주정 및 메탄올로 추출하였으며, 응애 방제 효과(%)는 아이비>란타나>예루살렘체리로 나타났다. 각 추출물은 GC/MS 분석을 통해 생리활성 후보 물질을 확인하였다. 이를 통해 농업 이익 증대, 생산량 유지와 더불어 환경 및 인간과 다른 유기체에 피해를 주지 않으며 효과적으로 응애를 방제 할 수 있는 천연 생물적 방제제로의 가치를 확인하였다.

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Occurrence and Biovar Classification of Bacterial Wilt Caused by Ralstonia solanacearum in Eggplant (Solanum melongena) (가지의 Ralstonia solanacearum에 의한 풋마름병 발생과 생리형의 분화)

  • Lim, Yang-Sook;Lee, Mun-Jung;Cheung, Jong-Do;Rew, Young-Hyun;Kim, Byung-Soo
    • Research in Plant Disease
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    • v.14 no.1
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    • pp.10-14
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    • 2008
  • Batcterial wilt caused by Ralstonia solanacearum is one of important and widespread diseases worldwide as well as in Korea. Bacterial wilt disease caused by R. solanacearum has been reported mainly in solanaceous crops including eggplant (Solanum melongena), tomato (Solanum lycopersicum), potato (S. tuberosum), and pepper (Capsicum annuum). A total of 48 strains of R. solanacearum from eggplant were collected during 2005 and 2006. They were confirmed as R. solanacearum by PCR amplification with primer pair flipcF/flipcR resulting in production of 470-bp DNA fragment. The 15 isolates exhibited pathogenicity on eggplant and tomato, but less virulent on pepper than other species. The biovar of collected isolates, which have been reported of five types worldwide, were classified as biovars 3 and 4 by physiological test. Biovar 4 was the dormant type without pathogenicity on eggplant rootstock, whereas biovar 3 had pathogenicity on eggplant rootstocks that is resistant to R. solanacearum, indicating necessity of breeding new rootstock with resistance to R. solanacearum biovar 3

The Effects on Antimicrobial and Cytotoxicity of Solanum Iyratum Fractions (배풍등 분획물의 항균 및 암세포 증식 억제효과)

  • Shin Mi-Ok
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.948-954
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    • 2005
  • In this study, we investigated antimicrobial and cytotoxicity effects to each fraction extracted from Solanum lyratum (SL), which were extracted methanol (SLM) and then the extract was fractionated into five different types : hexane (SLMH), ethyl ether (SLMEE), ethylacetate (SLMEA), butanol (SLMB) and aqueous (SLMA). The antimicrobial activity was analyzed by the paper disc method. Among the various solvent fractions, SLMEA showed the strongest antimicrobial activies. The cytotoxicity of SL fractions on HeLa, MCF-7, HT-29 and HepG2 cells was evaluated by MTT assay. Among various partition layers, SLMEE showed the strongest cytotoxic effects to all cancer cell lines. We also observed that quinone reductase (QR) was induced by all fraction layers of SL to HepG2 cells. Since the QR-induced effects of SLMEE on HepG2 cells at $160{\mu}g/ml$ concentration showed 2.1 when compared with a control value of 1.0, inducer of QR for cancer protection may be contained in this fraction.