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http://dx.doi.org/10.5352/JLS.2005.15.6.948

The Effects on Antimicrobial and Cytotoxicity of Solanum Iyratum Fractions  

Shin Mi-Ok (Dept. of Food and Nutrition, Silla University)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 948-954 More about this Journal
Abstract
In this study, we investigated antimicrobial and cytotoxicity effects to each fraction extracted from Solanum lyratum (SL), which were extracted methanol (SLM) and then the extract was fractionated into five different types : hexane (SLMH), ethyl ether (SLMEE), ethylacetate (SLMEA), butanol (SLMB) and aqueous (SLMA). The antimicrobial activity was analyzed by the paper disc method. Among the various solvent fractions, SLMEA showed the strongest antimicrobial activies. The cytotoxicity of SL fractions on HeLa, MCF-7, HT-29 and HepG2 cells was evaluated by MTT assay. Among various partition layers, SLMEE showed the strongest cytotoxic effects to all cancer cell lines. We also observed that quinone reductase (QR) was induced by all fraction layers of SL to HepG2 cells. Since the QR-induced effects of SLMEE on HepG2 cells at $160{\mu}g/ml$ concentration showed 2.1 when compared with a control value of 1.0, inducer of QR for cancer protection may be contained in this fraction.
Keywords
antimicrobial; cytotoxicity; quinone reductase; Solanum lyratum;
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