• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.644-651
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• 2013

The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormone-like activity. The material was found to harbor a bacterial community of $2.38{\times}10^8$ CFU/g dw dominated by Gram-positives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of ${\beta}$-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.

• 한국초지조사료학회지
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• 제28권3호
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• pp.165-170
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• 2008

티모시의 colt 품종을 이용하여 최적 조직배양조건을 확립하기 위하여 성숙종자를 배양함에 있어 첨가되어지는 식물 생장조절 물질의종류와 농도가 배발생 캘러스 유도 및 식물체재분화에 미치는 영향을 조사하였다. 배발생 캘러스 유도시 첨가되는 2,4-D는 3 mg/L 첨가 구에서 78%로 캘러스 유도율이 가장 높았으며, 3 mg/L 2,4-D와 0.1 mg/L의 BA를 첨가하여 배양했을 때 형성된 캘러스는 조직적으로 치밀하며 유백색의 배발생 캘러스가 가장 많이 형성되었다. Type B 캘러스만을 이용하여 식물체재분화에는 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 배지에 캘러스를 배양했을 때 25.3%의 재분화율을 나타내었다. 본 연구를 통하여 확립된 효율적인 재분화 시스템은 분자육종을 통한 신품종 티모시의 개발에 유용하게 이용될 수 있을 것이다.

• 한국자원식물학회지
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• 제35권1호
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• pp.29-35
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• 2022

변산향유는 꿀풀과의 국내 자생식물로 식물분류학회지에 처음으로 알려졌다. 초장은 20-35 cm로 작고 잎은 혁질로 윤택이 있다. 꽃은 11월에 보라색으로 개화하는 특징을 갖고 있어 분화 및 지피용 관상소재로 활용도가 매우 높다. 따라서 본 연구는 관상식물로 활용하기 위해 변산향유의 육묘 시 플러그 트레이 종류(128, 162, 200구), 토양조성(원예상토: 마사 1:1, 2:1, 4:1), 파종 립수(1, 2, 3립) 및 추비 농도(0, 250, 500, 1000 mg/L)의 수준을 구명하기 위해 수행하였다. 연구의 결과 변산향유는 트레이의 셀 크기가 커질수록 생육도 비례하여 증가하였다. 토양조성에 따른 생육은 마사토의 비율이 증가할수록 생육이 억제되었다. 셀 당 파종량에 따른 생육은 파종량이 많아질수록 초장, 초폭, 엽장, 엽폭이 감소하였으나 개체당 엽수, 줄기직경, 근장은 큰 차이가 없었다. 추비농도별 처리는 농도가 높아질수록 생육도 증가하는 경향을 보였다. 따라서 변산향유는 육묘시 128구 트레이에 원예상토를 충진한 다음 셀 당 1립 파종하여 재배하며, 추비는 비교적 고농도인 1000 mg/L로 엽면살포하여 육묘하는 것이 가장 효과적일 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.883-888
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• 2012

The efficacy of cowdung, Bangladesh Institute of Nuclear Agriculture (BINA)-biofertilizer, and Bangladesh Agricultural University (BAU)-biofungicide, alone or in combination, was evaluated for controlling foot rot disease of lentil. The results exhibited that BINA-biofertilizer and BAU-biofungicide (peat soil-based Rhizobium leguminosarum and black gram bran-based Trichoderma harzianum) are compatible and have combined effects in controlling the pathogenic fungi Fusarium oxysporum and Sclerotium rolfsii, which cause the root rot of lentil. Cowdung mixing with soil (at 5 t/ha) during final land preparation and seed coating with BINA-biofertilizer and BAU-biofungicide (at 2.5% of seed weight) before sowing recorded 81.50% field emergence of lentil, which showed up to 19.85% higher field emergence over the control. Post-emergence deaths of plants due to foot rot disease were significantly reduced after combined seed treatment with BINA-biofertilizer and BAU-biofungicide. Among the treatments used, only BAU-biofungicide as the seed treating agent resulted in higher plant stand (84.82%). Use of BINA-biofertilizer and BAU-biofungicide as seed treating biocontrol agents and application of cowdung in the soil as an organic source of nutrient resulted in higher shoot and root lengths, and dry shoot and root weights of lentil. BINA-biofertilizer significantly increased the number of nodules per plant and nodules weight of lentil. Seeds treating with BAU-biofungicide and BINA-biofertilizer and soil amendment with cowdung increased the biomass production of lentil up to 75.56% over the control.

• 한국유기농업학회지
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• 제12권3호
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• pp.249-264
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• 2004

High yield arable and crop farming demands a temporally and quantitatively determined application of plant nutrients according to field and culture. The nutrients may come from commercial fertilizers or from animal wastes. Regarding the dangers to soil, water and air, which come from current agricultural application measures for nitrogen, a sectoral approach for a nonpolluting liquid manure utilization can-not be used ally longer. An integrated system approach has to be found, leading to a drastic improvement of nutrient utilization and hence to a considerably reduced nutrient use. This can be only expected, if the organic manure can be applicated at times, when losses through leaching and volatilization can be minimized.

• Journal of Plant Biology
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• 제22권1_2호
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• pp.45-51
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• 1979

From 32 angiosperm species, pollen surface characters such as sculpture pattern, roughness, oil abundance and stickiness were subjectively scored, and compared with size and weight of the grains, and pollination mechanisms. There was a linear relationship between size and weight of the grains. Animal-pollinated grains showed significantly higher roughness, oil abundance and stickiness than wind-pollinated grains. In the former, the grain size was highly correlated with roughness and oil abundance. No such relationship was found in the latter. It was suggested, therefore, that the large the animal-pollinated grains, the greater is the stickiness required, where the stickiness depends on the surface soil abundance and roughness. No functional significance of the sculpture pattern could not be detected in this study.

• Journal of Forest and Environmental Science
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• 제40권1호
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• pp.43-52
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• 2024

Cadmium (Cd) is non-essential heavy metal that negatively affects plant metabolism. Nitric oxide (NO) is an increasingly important molecule for plant metabolism that makes signaling. In this study, it was aimed to investigate the alleviating effect of sodium nitroprusside (SNP) application as NO donor in white poplar (Populus alba) under Cd stress conditions. SNP and without SNP treatments increased the Cd accumulation in root tissue. While photosynthetic pigments (Chl a, Chl b, Chl a+b, and carotenoid) content decreased by only Cd application, SNP+Cd application decreased the rate of photosynthetic pigments reduction. When the results of Cd and Cd+SNP applications were evaluated for mineral (Fe, Zn, Mn and Cu) uptake, it was found that the positive effect of SNP was heterogeneously affected. Depending on SNP application, it was found that malondialdehyde (MDA) amount decreased in leaf in 100 µM Cd applications while hydrogen peroxide (H₂O₂) amount decreased in 100 and 500 µM Cd applications. When antioxidant enzyme activities were examined, it was found that catalase (CAT) and ascorbate peroxidase (APX) enzyme activities increased with 100 µM SNP applications under all Cd applications. As a result, it was found that SNP application under Cd stress generally supports physiological processes positively in white poplar, suggesting that NO molecule plays important alleviating roles in plant metabolism.

• 생물환경조절학회지
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• 제19권3호
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• pp.130-134
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• 2010

본 연구는 전북 고창의 수박 시설 농기에서 '우리꿀'을 대상으로 토양의 이화학성, 생육 및 과실품질에 대한 정식 전 심경로타리 처리의 효과를 알아보고자 수행하였다. 정식 전에 비해 재배 및 수확 후 토양 pH 변화는 거의 없었으나 토양 EC는 크게 떨어졌다. 유기물 및 유효인산 함량은 두 처리 모두에서 증가하였는데, 유효인산의 누적 함량이 심경로타리 처리구에서 적었다. 치환성 양이온도 Mg을 제외하고는 모두 감소한 경향이었는데 심경로타리 처리구에서 K의 감소가 뚜렷하였다. 수박의 정식 후 27일째 생육 특성 중 일반로타리 처리구에 비해 심경로타리 처리구에서 초장, 10절까지의 줄기 길이, 엽폭에서 유의하게 길었고, 1번 및 2번화의착과 절위가 낮았다. 그리고 육안 관찰 시 식물체 위조 증상 정도이 적었다. 수확기에는 일반로타리 처리구에 비해 심경로타리 처리구에서 30절까지의 줄기 길이가 유의하게 길었고, 육안 관찰 시 잎의 황변 현상이 적었다. 수확된 과실의 길이, 직경, 과피경도, 그리고 무게는 심경로타리 처리구에서 유의하게 높았다.

• 한국초지조사료학회지
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• 제27권4호
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• pp.235-240
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• 2007

페레니얼 라이그래스의 최적 조직배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 효율적인 식물체 재분화조건을 체계적으로 확립하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 9 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS 배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 5 mg/L BA가 첨가된 N6 배지에서 배양했을 때 58.3%의 높은 재분화율을 나타내었다. 본 연구를 통하여 확립된 단기간의 효율적인 재분화 시스템은 분자육종을 통한 신품종 페레니얼 라이그래스 개발에 유용하게 응용되어질 수 있을 것이다.

• 한국동물생명공학회지
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• 제35권1호
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• pp.28-34
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• 2020

Imazosulfuron is globally considered as a relatively safe herbicide that controls plant growth by interfering with amino acid synthesis. It is stable, persists in the soil, and has low toxicity; however, studies about the toxic effects of imazosulfuron on non-targeted aquatic vertebrates are scarce. In this study, imazosulfuron was able to induce acute lethality on zebrafish embryos within 48 h. Imazosulfuron also had adverse effects on heartbeats and induced abnormal development with pericardial edema and scoliosis. Moreover, apoptosis and oxidative stress were increased by imazosulfuron in a dose-dependent manner. Thus, all our results showed that imazosulfuron has toxic effects on zebrafish embryogenesis.