Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration of Perennial Ryegrass (Lolium perenne L.)

식물생장조절물질이 페레니얼 라이그래스 (Lolium perenne L.)의 캘러스 유도와 식물체 재분화에 미치는 영향

  • Published : 2007.12.31
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Abstract

Optimum tissue culture conditions for an efficient induction of embryogenic callus from mature seeds of perennial ryegrass (Lolium perenne L.) and regeneration of plants from callus tissues were investigated. MS medium containing 3 mg/L 2,4-D and 0.1 mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (58.3%) was observed when the embryogenic callus tissues were cultured on N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be helpful for molecular breeding of perennial ryegrass through Agrobacterium-mediated genetic transformation.

페레니얼 라이그래스의 최적 조직배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 효율적인 식물체 재분화조건을 체계적으로 확립하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 9 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS 배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 5 mg/L BA가 첨가된 N6 배지에서 배양했을 때 58.3%의 높은 재분화율을 나타내었다. 본 연구를 통하여 확립된 단기간의 효율적인 재분화 시스템은 분자육종을 통한 신품종 페레니얼 라이그래스 개발에 유용하게 응용되어질 수 있을 것이다.

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References

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