• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2002년도 춘계 국제 학술대회
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• pp.9-12
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• 2002

Diffusive transport of volatile organic compounds(VOCs) and their degradation by bacteria in unsaturated soils are couple by poorly understood mass transfer kinetics at the gas/water interface. Determination of the fate of VOCs in unsaturated soil is necessary to evaluate the feasibility of natural attenuation as a VOC remediation strategy. The objective of this study was to develop a mechanistically based mathematical model that would consider the interdependence of VOC transport, microbial activity, and sorptive interaction in a moist, unsaturated soil. Because the focus of the model was on description of natural attenuation, the advective VOC transport that is induced in engineered remediation processes such as vapor extraction was not considered. The utility of the model was assessed through its ability to describe experimental observations form diffusion experiments using toluene as a representative VOC in well-defined soil columns that contained a toluene degrading bacterium, Pseudomonas Putida, as the sole active microbial species. The coefficient for gas-liquid mass-transfer, K$\sub$LA/, was found to be a key parameter controlling the ability of bacteria to degrade VOCs. This finding indicates that soil size and geometry are likely to be important parameters in assessing the possible success of natural attenuation of VOCs in contaminated unsaturated soils.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2000년도 창립총회 및 춘계학술발표회
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• pp.108-111
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• 2000

FeS/FeS$_2$ minerals have been known to be potentially useful reductant to the removal of common organic contaminants in groundwater and soil. This research is aimed at improving our understanding of factors affecting the pathways and rates of reductive transformation of Hexachloroethane by catalytical iron minerals in natural system. Hexachloroethane is reduced by FeS/FeS$_2$ minerals under anaerobic condition to tetrachloroethylene and trichloroethylene with pentachloroethyl radical as the intermediate products. The kinetics of reductive transformations of the Hexachloroethane have been investigated in aqueous solution containing FeS, FeS$_2$. The proposed reduction mechanism for the adsorbed nitrobenzene involves the electron donor-acceptor complex as a precursor to electron transfer. The adsorbed Hexachloroethane undergo a series of electron transfer, proton transfer and dehydration to achieve complete reduction. It can be concluded that the reductive transformation reaction takes place at surface of iron-bearing minerals and is dependent on surface area and pH. Nitrobenzene reduction kinetics is affected by reductant type, surface area, pH, the surface site density, and the surface charge. FeS/FeS$_2$-mediated reductive dechlorination may be an important transformation pathway in natural systems.

• Journal of Radiation Protection and Research
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• 제16권1호
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• pp.33-42
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• 1991

Sr-90과 소석회를 처리한 pH 6.05의 양질사토로부터 보리에 의한 Sr-90 흡수 실험을 온실내에서 포트 재배로 실시하였다. 수확기에 보리 지 상부로의 Sr-90 흡수율은 늘쌀보리가 평균 0.41%, 올보리가 평균 0.23%였고 전이계수도 전자가 후자보다 부위에 따라 30-60% 정도 높았다. 흡수율과 전이 계수는 전체 적으로 볼 때 Sr-90 처 리 농도간에 유의 차가 인정 되지 않았다. 10a당 약 94kg에 해당하는 소석회 처리는 Sr-90 흡수를 억제하고 전이계수를 감소시키는데 효과적이지 못한 수준이었다. 보리의 부위별 Sr-90 전이 계수는 건조 중 기준으로 줄기 1.51, 및 4.45, 종실 0.35, 전지상부 1.30으로 제안될 수 있었다. Sr-90 흡수에 따른 작물체의 생육장해나 수량감소는 없었다.

• The Plant Pathology Journal
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• 제32권2호
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• pp.136-144
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• 2016

Pseudomonas fluorescens pc78 is an effective biocontrol agent for soil-borne fungal diseases. We previously constructed a P43-gfp tagged biocontrol bacteria P. fluorescens pc78-48 to investigate bacterial traits in natural ecosystem and the environmental risk of genetically modified biocontrol bacteria in tomato rhizosphere. Fluctuation of culturable bacteria profile, microbial community structure, and potential horizontal gene transfer was investigated over time after the bacteria treatment to the tomato rhizosphere. Tagged gene transfer to other organisms such as tomato plants and bacteria cultured on various media was examined by polymerase chain reaction, using gene specific primers. Transfer of chromosomally integrated P43-gfp from pc78 to other organisms was not apparent. Population and colony types of culturable bacteria were not significantly affected by the introduction of P. fluorescens pc78 or pc78-48 into tomato rhizosphere. Additionally, terminal restriction fragment length polymorphism profiles were investigated to estimate the influence on the microbial community structure in tomato rhizosphere between non-treated and pc78-48-treated samples. Interestingly, rhizosphere soil treated with strain pc78-48 exhibited a significantly different bacterial community structure compared to that of non-treated rhizosphere soil. Our results suggest that biocontrol bacteria treatment influences microbial community in tomato rhizosphere, while the chromosomally modified biocontrol bacteria may not pose any specific environmental risk in terms of gene transfer.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1130-1134
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• 2005

To monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or non-transgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at $25^{\circ}C$. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato.

• Geomechanics and Engineering
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• 제11권4호
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• pp.553-570
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• 2016

A series of three-dimensional (3D) parametric finite element analyses have been performed to study the influence of the relative locations of pile tips with regards to the tunnel position on the behaviour of single piles and pile groups to adjacent tunnelling in weathered soil. When the pile tips are inside the influence zone, which considers the relative pile tip location with respect to the tunnel position, tunnelling-induced pile head settlements are larger than those computed from the Greenfield condition. However, when the pile tips are outside the influence zone, a reverse trend is obtained. When the pile tips are inside the influence zone, the tunnelling-induced tensile pile forces mobilised, but when the pile tips are outside the influence zone, compressive pile forces are induced because of tunnelling, depending on the shear stress transfer mechanism at the pile-soil interface. For piles connected to a cap, tensile and compressive forces are mobilised at the top of the centre and side piles, respectively. It has been shown that the increases in the tunnelling-induced pile head settlements have resulted in reductions of the apparent factor of safety up to approximately 43% when the pile tips are inside the influence zone, therefore severely affecting the serviceability of the piles. The pile behaviour, when considering the location of the pile tips with regards to the tunnel, has been analysed in great detail by taking the tunnelling-induced pile head settlements, axial pile forces, apparent factor of safety of the piles and shear transfer mechanism into account.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.187-187
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• 2017

Cadmium (Cd) pollution is rapidly increasing in worldwide due to industrialization and urbanization. In addition to its negative effects on the environment, Cd pollution adversely affects human health. Rice (Oryza sativa L.) is an important agricultural crop worldwide, including South Korea, and studies have examined its ability to alleviate Cd uptake from the soil into plants. However, information about the relationship between sulfur (S) and antioxidants in rice seedlings is still limited with regard to Cd phytotoxicity. We therefore investigated the changes in reactive oxygen species (ROS) and antioxidants in rice (Oryza sativa L. 'Dongjin') seedlings exposed to toxic Cd, S treatment, or both. The exposure of rice seedlings to $30{\mu}M$ Cd inhibited plant growth; increased the contents of superoxide, hydrogen peroxide, and malondialdehyde (MDA); and induced Cd uptake by the roots, stems, and leaves. Application of S to Cd-stressed seedlings decreased Cd-induced oxidative stress by increasing the capacity of the glutathione (GSH)-ascorbate (AsA) cycle, promoted S assimilation by increasing cysteine, GSH, and AsA contents in treated plants, and decreased Cd transfer from the roots to the stems and leaves. In conclusion, S application of plants under Cd stress promoted Cys and GSH biosynthesis and GSH-AsA cycle activity, thereby lowering the rate of Cd transfer to plant shoots and promoting the scavenging of the ROS that resulted from Cd toxicity, thus alleviating the overall Cd toxicity. Therefore, these results provide insights into the role of S in regulating the tolerance, uptake, and translocation of Cd in rice seedlings. The results of this study indicate that S application should have potential as a tool for mitigating Cd-stress in cereal crops, especially rice.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.376-383
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• 2005

Alcaligenes sp. JMP228 carrying 2,4­dichlorophenoxyacetic acid (2,4-D) degradative plasmid pJP4 was inoculated into natural soil, and transfer of the plasmid pJP4 to indigenous soil bacteria was investigated with and without 2,4-D amendment. Plasmid pJP4 transfer was enhanced in the soils treated with 2,4-D, compared to the soils not amended with 2,4-D. Several different transconjugants were isolated from the soils treated with 2,4-D, while no indigenous transconjugants were obtained from the unamended soils. Inoculation of the soils with both the donor Alcaligenes sp. JMP228/pJP4 and a recipient Burkholderia cepacia DBO 1 produced less diverse transconjugants than the soils inoculated with the donor alone. Repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) analysis of the transconjugants exhibited seven distinct genomic DNA fingerprints. Analysis of 16S rDNA sequences indicated that the transconjugants were related to members of the genera Burkholderia and Pandoraea. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that inoculation of the donor caused clear changes in the bacterial community structure of the 2,4-D­amended soils. The new 16S rRNA gene bands in the DGGE profile corresponded with the 16S rRNA genes of 2,4-D­degrading transconjugants isolated from the soil. The results indicate that introduction of the 2,4-D degradative plasmid as Alcaligenes sp. JMP228/pJP4 has a substantial impact on the bacterial community structure in the 2,4-D-amended soil.

• Geomechanics and Engineering
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• 제6권6호
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• pp.517-530
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• 2014

Seismic ground response analysis is one of the most important issues in geotechnical earthquake engineering. Conventional seismic site response and free field analysis of layered soils does not consider the effect of surcharge mass which may be present on the top layer. Surcharge mass may develop extra inertial force to the soil and, hence, significantly affect on the results of seismic ground response analysis. Methods of analysis of ground response may also be categorized into time domain and frequency domain concepts. Simplicity in developing analytical relations and accuracy in considering soil dynamic properties dependency to loading frequency are benefits of frequency domain analysis. In this part of the paper, seismic ground response is analyzed using transfer function method for soil layers considering surcharge mass on the top layer. Equation of motion, wave equation, is solved using amended boundary conditions which effectively take the impact of surcharge mass into account. A computer program is developed by MATLAB software based on the solution method developed for wave equation. Layered soils subjected to earthquake loading were numerically studied and solved especially by the computer program developed in this research. Results obtained were compared with those given by DEEP SOIL computer program. Such comparison showed the accuracy of the program developed in this study. Also in this part, the effects of geometrical and mechanical properties of soil layers and especially the impact of surcharge mass on transfer function are investigated using the current approach and the program developed. The efficiency and accuracy of the method developed here is shown through some worked examples and through comparison of the results obtained here with those given by other approaches. Discussions on the results obtained are presented throughout in this part.

• 대한토목학회논문집
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• 제28권2C호
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• pp.133-141
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• 2008

토목섬유보강 성토지지말뚝시스템에서 지반아칭에 의한 하중전이 특성을 규명할 수 있는 이론해석법을 개발하였다. 이론해석 결과, 성토지지말뚝에 토목섬유가 복합시공되면, 말뚝의 효율이 증가하였으며, 효율의 증대효과는 지반아치가 완전히 발달하기 이전인 저성토 구간에서 특히 두드러지게 나타났다. 또한, 이론해석법에 의한 하중전이는 말뚝의 간격, 성토고, 지반정수 및 토목섬유에 크게 영향을 받고 있다. 즉, 말뚝의 간격이 증가하면 말뚝의 효율은 감소하며, 성토고, 성토지반의 내부마찰각 및 토목섬유의 강도가 증가하면 효율이 커지게 된다. 이와같은 해석결과는 본 제안식이 토목섬유보강 성토지지말뚝시스템에서의 지반아칭효과를 잘 설명해주는 합리적인 해석법임을 나타내고 있다.