• Journal of Biosystems Engineering
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• v.26 no.5
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• pp.423-430
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• 2001

The purpose of this study is to develop high-speed rotary tillage system for a power tiller by improving the rotary blade and the power train of transmission. Mechanical structure of gear train of rotary drive of conventional power tiller was simplified so that power can be transmitted directly from second shaft to tilling speed change shaft by rotating freely the transfer gear which changes the direction of rotation of shafts using needle bearing installed into middle shaft. A new gear train suitable for the single-edged rotary blade and high-speed rotary drive was developed with the rotational speed of rotary shaft faster than 7.5% at 1st-speed and 1.4% at 2nd-speed the one of conventional system by changing the numbers of teeth of gears of middle shaft, tilling speed change shaft and PTO shaft. Using the developed gear train for high-speed rotary drive, field tests were performed to compare tillage performances by the developed single-edged blade and by the conventional double-edged blade. The results showed that the performances by the single-edged blade compared with the one by the double-edged blade was improved about 18% in field capacity, about 34% in fuel consumption, and 9.4% in soil crushing ratio. Therefore, it may be concluded that tillage performance by the single-edged blade was improved compared to the one by the conventional blade. Evaluation of the developed system consisting of single-edged blade and gear train for high-speed rotary drive in field revealed that tillage performance of the developed system was similar to the one of field test conducted using the system consisting of single-edged blade and gear train for rotary drive of conventional power tiller However, considering the higher cone index of the upland field where evaluation was carried out compare to the one of the ordinary paddy field, it may be concluded that tillage performance of the developed rotary tilling system better than the one of conventional system.

• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.315-318
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• 1994

To obtain transformed plants, we cocultured cotyledonary explants of Codonopsis lanceolata with Agrobacterium tumefaciens LBA4404, a disamed strain harboring a binary vector pBI121 carrying the CaMV35S promoter-$\beta$-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker in MS liquid medium with 1mg/L BA. After 48 h of culture, explants were transferred onto MS solid medium with Img/L BA, 250mg/L carbenicillin, and 100mg/L kanamycin sulfate and cultured in the dark. Numerous adventitious buds formed on the cut edges of the explants after 2 weeks of culture. When subjected to GUS histochemical assay buds showed a positive response at a frequency of 15%. Explants formed adventitious shoot at a frequency of 56.7%, after 6 weeks of culture. Upon transfer onto the basal medium, most of the shoots were rooted and subsequently the regenerants were transplanted to potting soil. Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.185-188
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• 2002

Off-white, friable embryogenic calluses were formed on the internal integument of mature seeds of yuzu (Citrus junos) cultured on Murashige and Skoog's basal medium at a frequency of 1.2%. Embryogenic calluses were proliferated when cultured on medium with 1 mg/L 2,4-D. Upon transfer to medium with 0.1 mg/L kinetin, embryogenic calluses produced numerous somatic embryos. Embryogenic suspension cultures were established by placing embryogenic calluses into liquid medium with 1 mg/L 2,4-D. When plated onto medium with 0.5 mg/L ABA, embryogenic cells developed into somatic embryos at a high frequency, and then regenerated into plantlets. Plantlets were successfully transplanted to potting soil and grown in a greenhouse.

• Plant Biotechnology Reports
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• v.3 no.3
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• pp.205-211
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• 2009

An efficient in vitro regeneration protocol for moth bean [Vigna aconitifolia (Jacq.) Marechal] via somatic embryogenesis has been developed. Embryogenic callus cultures were established from the cotyledonary node as explant on semi-solid Murashige and Skoog (MS) medium supplemented with $0.75mg\;1^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D) and $1.5mg\;1^{-1}$ 6-benzylaminopurine (BA) and with various additives ($50mg\;1^{-1}$ ascorbic acid and $25mg\;1^{-1}$ each of adenine sulphate, citric acid and $_L-arginine$). Numerous somatic embryos differentiated on MS basal nutrient medium supplemented with $0.25mg\;1^{-1}$ 2,4-D and $0.5mg\;1^{-1}$ of kinetin (Kin). Sustained cell division resulted in the formation of cell aggregates, which progressed to the globular- and heart-shaped somatic embryos and then, if they differentiated properly, to the torpedo shape and cotyledonary stages. The transfer of embryos onto fresh MS basal medium containing $0.2mg\;1^{-1}$ BA and $2.0mg\;1^{-1}$ gibberellic acid enabled the embryos to achieve complete maturation and germination. More than 80% of somatic embryos were converted into true-to-type fertile plants. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil.

• Korean Journal of Organic Agriculture
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• v.7 no.2
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• pp.125-151
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• 1999

After the release of facts that the high nitrate contents in vegetables by Korean organic farming, organic farmers were recommended by Korean Organic Farmer Association to apply 20t/ha compost while they applied before as much as they could, sometimes even more than 100t/ha. This study was aimed to check the safety of Korean organic product in terms of nitrate content. Current nitrate content in leaf and root vegetables by 3 different farming systems(organic farming, conventional farming, greenhouse farming) were monitored and furthermore it was compared with previously published another data in Korea to find out any changes among the monitored years(1993-1999). Current nitrate content in chinese cabbage, lettuce and kale became less compare to those of the beginning of monitoring, and especially those cultivated organic farming got the lowest value while previous organic vegetables at beginning of 90's showed the higher value than those cultivated in greenhouse. This decrease in organic vegetables was affected by less application rate of organic fertilizer than before at 20t/ha. But the nitrate level by organic farming was still higher compare to those cultivated by conventional farming in open field, since currently monitored organic vegetables were cultivated basically in greenhouse condition. It shows the organic farmer the necessity of transfer their farming site and condition from greenhouse to open field in order to decrease of its high level of nitrate caused long-term application and slow release characteristics of organic fertilizer. It was concluded the adoption of soil nitrate test to recommend a organic farmer the exact application rate for need of crop growth. Additionally the mechanism of lower nitrate accumulation in rice and fruit vegetables were also discussed in the paper.

• Journal of the Korean Geotechnical Society
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• v.33 no.2
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• pp.35-47
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• 2017

Thermal behavior of soils is mainly focused on thermal conduction, and the study of natural convection is very limited. Increase of soil temperature causes natural convection due to buoyancy from density change of pore water. The limitations of the analysis using fluid dynamics for natural convection in the porous media is discussed and a new numerical analysis is presented for natural convection in porous media using THM governing equations fully coupled in the macroscopic view. Numerical experiments for thermal probe show increase in the uncertainty of thermal conductivity estimated without considering natural convection, and suggest appropriate experimental procedures to minimize errors between analytical model and numerical results. Burial of submarine power cable should not exceed the temperature changes of $2^{\circ}C$ at the depth of 0.2 m under the seabed, but numerical analysis for high permeable ground exceeds this criterion. Temperature and THM properties of the seafloor are important design factors for the burial of power cable, and in this case effects of natural convection should be considered. Especially, in the presence of heat sources in soils with high permeability, natural convection due to the variation of density of pore water should be considered as an important heat transfer mechanism.

• Korean Journal of Remote Sensing
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• v.11 no.1
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• pp.65-80
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• 1995

Remote Sensing data with ancillary ground-based meteorological data provides the capalility of computing threeof the four surface energy balance components(i.e. net radiation, soil heat flux and sensible heat flux) at different spatial and temporal scales. As a result, this enablis the estimation of the remaining term, latent heat flux. One of the practical applications with this approach is to produce evapotranspiration maps over large areas. This results could estimate and reproduce areal evapotranspiration over large area as much as several hundred sequare kilometers. Moreover, some calculating simulations for the effects of the land use change on the surface heat flux has been made by this method, which is able to estimate evapotranspiration under arbitracy presumed condition. From the simulation of land use change, the results suggests that the land use change in study area can be produce the significant changes in surface heat flux. This preliminary research suggests that the future research should involve development of methods to account for the variability of meteorological parameters brought about by changes in surface conditions and improvements in the modeling of sensible heat transfer across the surface atmosphere interface for partical canopy conditions using remote sensing information.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.525-525
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• 2015

국립기상과학원은 국가 물관리를 효율적으로 지원하기 위하여 TOPLATS(TOPmodel based Land-Atmosphere Transfer Scheme) 지면모델 기반을 활용한 전국 수문기상 분석 및 예측정보 생산체계를 구축하였다. TOPLATS 지면모델에서는 토양, 식생 등을 표현하기 위한 다양한 매개변수들이 사용되고 있으며, 그 중에서도 토양 속성과 관련 매개변수들은 토양수분, 증발산 등의 수문기상요소 생산에 큰 영향을 미치고 있어 현실적인 토양 특성에 대한 고려가 요구된다. 본 연구는 국립농업과학원의 토양도 정보를 이용하여 TOPLATS 지면모델에서 요구되는 토양 속성 및 관련 매개변수를 산정하고 이를 모델에 적용하고자 하였다. TOPLATS 모델에 사용되는 토양 매개변수는 총 22개 이며, 본 연구에서는 국립농업과학원에서 제공한 총 405개의 토양통에 대한 매개변수를 각각 산정하였다. TOPLATS 모델을 강제하기 위한 기상자료는 동네예보 분석자료, KLAPS(Korea Local Analysis and Prediction System) 분석자료, 입사 단 장파 복사량은 ASOS 관측자료를 기반으로 한 5km 해상도의 남한 격자자료이며, 2010~2013년 기간의 토양수분, 증발산량에 대한 검증 연구를 수행하였다. 본 연구의 결과는 기존의 11개 토양속성정보로 산출된 결과와 비교 분석하여 추후 제시할 예정이며, 본 연구에서 산출된 국내 토양 특성을 반영한 고해상도 수문기상정보는 향후 홍수 예측 및 가뭄 평가에 활용 할 수 있을 것으로 기대된다.

• Korean Journal of Environmental Agriculture
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• v.40 no.1
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• pp.67-72
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• 2021

BACKGROUND: Biological iron redox transformation alters iron minerals, which may act as effective adsorbents for arsenate [As(V)] in the environments. In the viewpoint of alleviating arsenate, microbial Fe(III) reduction was sought under high concentration of As(V). In this study, Fe(III)-reducing bacteria were isolated from the wild plant rhizosphere soils collected at abandoned mine areas, which showed tolerance to high concentration of As(V), in pursuit of potential agents for As(V) bioremediation. METHODS AND RESULTS: Bacterial isolation was performed by a series of enrichment, transfer, and dilutions. Among the isolated strains, two strains (JSAR-1 and JSAR-3) with abilities of tolerance to 10 mM As(V) and Fe(III) reduction were selected. Phylogenetic analysis using 16S rRNA genesequences indicated the closest members of Pseudomonas stutzeri DSM 5190 and Paenibacillus selenii W126, respectively for JSAR-1 and JSAR-3. Ferric and ferrous iron concentrations were measured by ferrozine assay, and arsenic concentration was analyzed by ICP-AES, suggesting inability of As(V) reduction whereas ability of Fe(III) reduction. CONCLUSION: Fe(III)-reducing bacteria isolated from the enrichments with arsenate and ferric iron were found to be resistant to a high concentration of As(III) at 10 mM. We suppose that those kinds of microorganisms may suggest good application potentials for As(V) bioremediation, since the bacteria can transform Fe while surviving under As-contaminated environments. The isolated Fe(III)-reducing bacterial strains could contribute to transformations of iron minerals which may act as effective adsorbents for arsenate, and therefore contribute to As(V) immobilization

• Journal of Practical Agriculture & Fisheries Research
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• v.17 no.1
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• pp.183-204
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• 2015

Papua New Guinea, birthplace of the South Pacific, is a natural nation which have potential of increasing crops output because it has optimum condition for crop growth as tropical rain forest climate under hot and humid climate. Farming village of Papua New Guinea want to produce crops for create income beyond the self-sufficiency. It needs the technological transfer such as irrigation facilities and understanding of agricultural weather condition for good crops production. In particular, it needs a improvement through pH, EC, ORP for make optimum soil condition and it need the standardization production and farm products what the consumer wants. Internationally technical cooperation is needed for agricultural development of Papua New Guinea and maintenance of international cooperation will help for economic development between the two countries. In particular, basic environment research for agricultural development of Papua New Guinea is expected to play a larger role of technical cooperation of agriculture.