• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.137-142
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• 1998

Helicobacter pylori is a Gram-negative bacterium which causes chronic gastritis and is associated with gastric ulcer, duodenal ulcer and gastric carcinoma. In the process of screening of antibacterial activities against H. pylori from soil microorganisms, fungus No. 60686 was isolated. After fermentation of No.60686, the antibacterial compound was isolated, purified and followed by extraction of mycelium with organic solvents, acetone and ethyl acetate, through silica gel chromatography, LH-20 gel chromatography and HPLC. As a result of the structural analyses of the compound by IR, $^1$H- and $^{13}$C-NMR, FAB/Mass spectrophotometer, the compound having the antimicrobial activity was identified as chaetoglobosin A ($C_{32}H_{36}N_2O_5$), a cytochalasan derivative. The antimicrobial activity of chaetoglobosin A was tested against Gram-positive and negative bacteria by paper disk method. Among the test strains of 9 Gram-positive bacteria and 18 Gram-negative bacteria containing 4 H. pylori strains, the growth of 4 H. pylori strains and 3 S. aureus strains (SG 511, 285 and 503) was only inhibited by chaetoglobosin A. Also it was shown that its growth inhibition against H. pylori strains was stronger than that against S. aureus strains at the treatment of the same concentration. Therefore it was concluded that chaetoglobosin A has a specific growth inhibition against H. pylori of the tested bacteria.

• Korean Journal of Environmental Agriculture
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• v.24 no.4
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• pp.334-340
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• 2005

This experiment was conducted to evaluate the nutrient loss and to assess the eutrophication into small stream by intensive rains in the steeped cornfield during cultivation. The crop cultivated was a soiling com (DW5969), and the experimental plots were divided into two parts that were 10 and 18% of slope degrees. The amount of T-N and T-P loss was calculated by analysis of surface run-off water quality, and was investigated the effect of eutrophication to small stream as a part of life cycle assessment (LCA) methodology application. For the surface run-off water quality, EC and T-N values were highest in first runoff event as compared to the other events and maintained the stage state with litter variations at every hour during the runoff period except for EC in the slope 18%. However, T-P concentration has been a transient stage after runoff event of July 27. Total surface run-off ratio was not significantly different with slope degrees, but amount of T-N and T-P losses at 18% of slope were high as $5.96kg\;ha^{-1}\;and\;0.65kg\;ha^{-1}$ as relative to 10% of slope degree, respectively. Furthermore, T-N losses from run-off water in the sloped cornfield 10 and 18% were approximately 9.8 and 12.5% of the N applied as fertilizer when the fertilizer applied at recommended rates after soil test, respectively. For the eutrophication impact to the small stream, it was shown that $PO_4$ equivalence and Eco-indicator value at 18% of slope degree were greater as much $6.11kg\;ha^{-1}$ and 0.81 as compared to the slope angle 10%, respectively. Therefore, it was appeared that each effect of nutrient losses, eutrophication and Eco-indicator value was enhanced according with higher slope degree.

• Research in Plant Disease
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• v.10 no.4
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• pp.331-336
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• 2004

The fungus genus Sclerotinia contains a number of important plant pathogens. Vegetable growers in our country are probably most familiar with Sclerotinia sclerotiorum, the causes of sclerotinia rot on crisphead lettuce. S. sclerotiorum has a wide host range which can include lettuce as well as crops such as broccoli, cabbage, carrots, celery, beans, peppers, potatoes, stocks, and tomato. Some fungicides, including benomyl, are effective in some crops, but not all. So, we isolated a antagonistic bacteria that are active on sclerotinia rot caused by S. sclerotiorum and that can be used to control it. About 702 strains had been isolated from soil around plant roots in the field. Ten strains showed strong antifungal activity against S. sclerotiorum. In pot test for antagonistic activity, A-7 strain showed high control value against the pathogen when compared with others. The strain was, therefore, selected as a biocontrol candidate against sclerotinia rot and its biochemical properties and 16S rDNA sequence was analyzed. The A-7 strain was highly related to Bacillus subtilis and B. amyloliquefaciens. To confirm precise identification, we had performed gyr A gene sequences analysis. Its sequence had 96% similarity with B. amyloliquefaciens. Consequently, the isolate was identified as B. amyloliquefaciens A-7.

• Journal of Preventive Medicine and Public Health
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• v.27 no.4 s.48
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• pp.693-709
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• 1994

This epidemiologic study was carried out to investigate cauuse and magnitude of food-poisoning like epidemic occurred among inhabitants of a village who have eaten dead cow's meat near Kyongju in February of 1994, around lunar new year, The investigation consisted of interview survey on all inhabitants of 77 households (111 males and 119 females) and their visitors (40 males and 35 females), skin test with anthraxinum (Russian product), study on clinical characteristics for the patients hospitalized, and microbiologic examination on microbes isolated from cow's meat, patient and soils of dead cow's barn. The results obtained are as followings; 1. The proportion of the inhabitants who ingested the dead cow's meat was 36.4%. The incidence rate of the disease was 65.1% for males, 41.7% for females and the cases were distributed evenly for all age groups. The group ingested raw meat showed higher incidence than the group ingested cooked meat. There was no case among people who did not eat the meat. 2. The most clinical symptoms were significantly more frequent among cases than non-cases : sore throat (57%), nausea (51%), fever (47%), indigestion (43%), cough (41%), anorexia (41%), abdominal distention (41%), and abdominal pain (39%) were the major symptoms among cases. 3. Among 29 cases hospitalized out of total 61 cases, three patients, all old and feeble persons, deceased from the disease resulting in 4.9% fatality rate among total patient and 10.3% among hospitalized. Septicemia and meningitis were the causes of the deaths. 4. Three strains isolated from patients, and three strains from dead cow's meat and soil revealed typical microbiologic characteristics of Bacillus Anthracis, which also proved to be fatal to experimentally infected mice.

• Journal of Aquaculture
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• v.13 no.3
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• pp.223-230
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• 2000

A rearing experiment of the olive flounder was performed in a set of water-reuse system to test the reusability of the water in culture system with (i) a foam fractionator to separate particles from water and (ii) a culture tank contain mineral particles to filter the metabolic wastes by adsorption and/or decomposition. Two kinds of commercially processed loess particles and a dolomite particle (all 50 ${\mu}$diameter) were tested. The mineral particles were suspended in the culture tank and the water was pumped into the foam fractionator, where the particles were separated and drained out with foam from the system. In a circular culture tank of 4.8 m in diameter with 10 d water, the juvenile olive flounders (23.1 g/fish, 5,555 fish, 128 kg total body weight) were stocked. 90 % of the rearing water was reused and turnover rate of the water in the tank was two times per hour. Water temperature was maintained 17${\pm}$1$^{\circ}C$. At the end of 75 day-experimental rearing, 5,532 flounders, weighing 468 kg, were harvested. An individual flounder grew to 84.6 g of body weight. The final stocking density was 26.0 kg/$m^2$. No diseases were observed during the experiment.

• The Korean Journal of Mycology
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• v.9 no.3
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• pp.133-139
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• 1981

Two hundred forty seven isolates obtained from 21 white or cream-colored mushroom strain by single spore isolation or multiple spore germination were compared type of mycelial growth in vitro and yield trial in a preliminary test. As a result of these tests, four isolates were selected and compared the yields of sporophores with those of 505 and 702 which are leading strains in mushroom production. The newly selected isolate No. 705 showed high yield of mushroom with good quality as described below. 1. The isolate No. 705 produced 13% more mushrooms than those from the strain No. 703. Both produced creamy type of mushroom. The isolate No. 705 showed high blanching yield ratio and moderate resistance to Mycogone perniciosa. 2. For the isolate No. 705 obtained by multiple spore germinations, the optimum temperature of mycelial growth was $25^{\circ}C$, also the mycelial growth was better at $15^{\circ}C$ than others, optimum moisture content of the compost was 65% and optimum casing soil pH for mycelial growth was 7.8. 3. The new isolate No. 705 produced more number of sporophores and the ratio between parts of sporophores were intermediate of those from the strains No. 505 and No. 703.

• The Korean Journal of Mycology
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• v.30 no.1
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• pp.44-49
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• 2002

The study was conducted to isolate and identify insecticidal bacteria for biological control of larvae of mushroom fly, Lycoriella mali, which is one of serious pests to oyster mushrooms during its cultivation period. Among eight bacteria isolated from the soil in the oyster mushroom beds and the dead body of L. mali, two bacteria, Bti-D and Bti-U showed more toxicity with mortality rate than other six-bacteria isolates. The two bacteria showed more toxicity in three instar of the period of development of the mushroom fly than in other instar. Symptoms of the larvae of L. mali infected by the two bacteria developed as follows: at the early infection, the front middle gut changed color to light brown, the middle gut to brown, whole body to black brown, and eventually, the fly died. For the identification of these isolates, cultural and biochemical characteristics by Bergey's manual and Biolog system, cell morphology by TEM, endospore and endotoxin by phase-contrast microscope, and test using 33H antisera were examined. According to the results, these two isolates, Bti-D and Bti-U were identified as Bacillus thuringiensis subsp. israelensis respectively.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.142-148
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• 2001

An indigenous antagonistic bacterium Bacillus sp. 7079 was isolated from a local soil sampled at Kyongju area in Korea . The strain has strong antagonistic ability which was originated from multifunctional mechanisms of chitinase and antibiotic and is a powerful antagonistic biocontrol agent against red-pepper rotting fungus Phytophthora capsici and Wilt fungus Fusarium oxysporum. The chitinase might degrade the cell wasll for Fusarium species. The selected Bacilus sp. 7079 was identified as a Bacillus amyloliquefaciens 7079. The maximal production of the chitinase from B, amyloliquefaciens 7079 were obtained in chitin-yeast extract medium containing 0.7%, $K_2$$HPO_4$, $0.2KH_2$$PO_4$, 0.1% ($NH_4$)$_2$$SO_4$, 0.05% sodium cirate, 0.01% $MgSO_4$$7H_2$O, 0.1% yeast extract and 0.1% colloidal chitin after cultivation of 3 days at pH 7.0 and $30^{\circ}C$. The best carbon and nitrogen sources for the production of the chitinase from B amyloliquefaciens 7079 were determined to be 0.1% colloi- dal chitin and 0.15% proteose peptone NO 3 respectively, The antagonistic activity of B amyloliquefaciens 7079 was confirmed using P. capsici by in vivo pot test with red-pepper plant.

• 한국지구물리탐사학회:학술대회논문집
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• 2005.09a
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• pp.93-116
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• 2005

SPT-Uphole tomography method was introducedand verified in this paper. In SPT-Uphole method, SPT (Standard Penetration Test) which is common in site investigation, was used as a source and several surface geophones in line were used as receivers. Shear wave velocity (Vs) distribution map which has triangular shape around the boring point can be obtained by tomography inversion. The factors for obtaining reliable result of SPT-Uphole tomography are exact travel time information and accurate inversion method. To establish of the SPT-Uphole tomography procedure, the most reliable method for obtaining exact travel time information and verification of tomography inversion method were studied by using theoretical travel time information and finite element method (FEM) analysis. finally, SPT-Uphole tomography method was performed at the weathered soil site in Kimje. By comparing with several boring data including SPT-N value, feasibility of this method was verified in the field.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.30-37
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• 2013

Analysis of new approaches to achieve naturally good ecological potential of forest road cut-slope by making the best use of advantages of gabion systems with vegetation base materials to prevent slope failure and erosion, in the area with highly erodible soil. Existing gabion systems can be divided into monolithic and modular system and can be divided into ten subtypes according to the purpose of establishment and combination of other measures. As a result on the monitoring of erosion amount from forest road cut-slopes in the test applications, the order of erosion amount from largest to smallest is as follows: the curved road cut-slope site where normal gabion system was established 5,840 $cm^3$; the control site 5,833 $cm^3$; the straight road cut-slope site where normal gabion system was established 5,621 $cm^3$; the curved road cut-slope site where the new gabion system was established 4,298 $cm^3$; and the straight road cut-slope site where the new gabion system 4,117 $cm^3$. Therefore, the result shows that the new gabion system is more effective than the normal gabion system to reduce erosion amount from forest road cut-slopes. During the study period, vegetation coverages of the straight and curved road cut-slope site where the new gabion system was established were about 56(30~85)% and about 45(28~65)%, so average vegetation coverage of the sites where the new gabion systems was established was higher than the sites where the normal gabion systems was established. Therefore, it was concluded that the new gabion system can be more effective for cut-slope revegetation.