• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.244-250
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• 1990

A bacterium capable of hydrotyzing raw starch was isolated from soil, which was identified as a strain of Bacillue. The effects of culture conditions and medium compositions on the enzyme production were investigated. Among tested carbon sources, soluble starch and wheat starch were most effective for the production of the enzyme, and the level of concentration for the optimal enzyme production was 0.5%. For nitrogen sources, polypeptone was best for the enzyme production, with the level of 0.5%. The enzyme was maximally produced by cultivating the organism at medium of initial pH 6.5, and temperature of $35^{\circ}C$. The enzyme was partially purified by Sepharose CL-6B gel filtration and DEAESephacel ion-exchange chromatography. The optimal pH and temperature for the enzyme reaction were 6.5 and $70^{\circ}C$, respectively. The enzyme most stable at pH 8.0, and temperature up to $60^{\circ}C$. In kinetic studies, the k, values for corn, wheat, rice and potato starch were 1.7, 1.4,2.5 and 1.090, respectively.

• Journal of the Korean Society of Groundwater Environment
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• v.5 no.2
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• pp.66-79
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• 1998

This study purposes to elucidate the characteristics of local water quality and the evolutional process of the spring water have been studied with the samples from 55 selected springs, from the coast region to the Baegrogdam, a caldera lake of Halla mountain in Cheju Island. Results of hydrochemical analyses in the 55 spring water show that Gwaneumsa is pollued with high hydrogen concentration, and spring waters from Yusuarmchun, Jangsumul, Dukjisemmul, Sinch'onk'nmul, Youngchunsa, Woonyangchun, Harwontongmul, Dongheamul, Seakdalsengsu, Andukkyekok, Dotimul, Kinmul, Harkangmul and Jungkeummul are polluted by the pollution sources from the surface surrounding ground. Spring waters of Samyang3suwon, Meungbusa, Sernurungmul, Tosanmul, Jungkeummul, Kwakgimul, Aewolharmul, Konemul and Nokgomul are also polluted from the sea water intrusion. The electrical conductivity, pH and the concentration of nitrate nitrogen and bicarbonate increase when the precipitation increases, but the concentration of sodium, chloride and sulphate decrease when the precipitation increases. The concentrations of potassium, magnessium and calcium are nearly independent of the precipitaion. Quality characteristics of spring water show complicated group of spring water through piper's trilinear diagram. The high mountain region consists of groups of sodium or potassium type and bicarbonate type; the middle mountain region consists of groups of sodium or potassium type and no dominant type; the low mountain consists of groups of no dominant type and sulfate or nitrate type; the coast region consists of groups of sodium or potassium type and chloride type or no dominant type. These characteristics indicate that the spring waters are changed from bicarbonate type in the high and middle mountain regions into non-bicarbonate type in the coastal region, as the precipitated waters flow downslope.

• Korean Journal of Weed Science
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• v.1 no.1
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• pp.5-14
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• 1981

Weed is one of the problems in the crop land as well as in uncultivated land, raising the farm management costs. Therefore, the weed control is essential for effective agricultural management. The cost for weed control in Korea occupies on the average 27.6% of the total labor cost required. Agricultural policies since 1960 were transferring from yield increase due to land productivities to increase of income due to labor productivities. Therefore, the weed control by hand is also changed to weed control by chemicals. The weed control by chemicals has also brought about some side-effects and needs better, improved weed control methods. The present weed control situation and related problems were studied to present new approaches for agricultural development in the future. There were 458 species of weeds in 82 families which were growing in the crop land. The weeds to control, however, are 12 in paddy field and 9 in upland. So far weeds in paddy field are well under control, while weeds in upland are poorly controlled due to change in chemical efficiency and chemical damage in the upland. The administration, research and extension work for the efficient use of agricultural chemicals have been done by various institutions, such as Office of Rural Development, Office of Forestry, and chemical companies. The courses for agricultural chemicals were offered in the agricultural colleges. However, the efficiency of chemicals could not be maximized due to the poor relationships among related institutes. The newly established Agricultural Chemical Research Center at the Office of Rural Development and the Korean Weed Science Association are expected to contribute toward improving weed control in Korea. The Korean agriculture in the future will eventually be mechanized and the varieties resistant to high nitrogen application and to high plant density will be required for high yields. The rice will be transplanted earlier and the whole growing period will be extended. The application of organic matter will be increased for increasing soil fertility, and the use of agricultural chemicals will be continued. Under such a condition, the studies on the weed occurrence and its integrated control measures will be needed. Also weed controls in the newly exclaimed land, crop varieties, horticultural varieties, forage crops, and forests are also needed to study. Basic and practical researches for the weed control to improve the labor productivity will be also needed. In order to meet the all requirements for efficient weed control, weed control systems including all the academics, research and extension workers, administratives, farmers and companies should be established. Also securing researchers and education of personnels are pre-required and research funds for the chemical studies should be provided efficiently and timely.

• Applied Biological Chemistry
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• v.44 no.4
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• pp.240-245
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• 2001

Suppression of nitrate accumulation in vegetables through foliar application of micronutrients was investigated. Spinach and lettuce were grown in pots under greenhouse condition. Micronutrient solutions containing Cu, Mn, Mo, and Zn were used; chitosan was added into one and the other contained chitosan oligomers. The micronutrient solutions were sprayed on the leaves at 3 and 4 weeks after transplanting of 20-day-old seedlings. Plants were harvested at 5-weeks after transplanting. Yield, contents of chlorophyll, Brix value, micronutrient, and nitrate, and nitrate reductase activity were measured. Fresh weights of lettuce and spinach were significantly increased by the foliar application of micronutrients. Contents of chlorophyll and micronutrients were higher in micronutrient-treated plants, while those of nitrate were reduced by about 10 and 14-23% in lettuce and spinach, respectively. Compared to the control plants, nitrate reductase activity was higher in plants treated with micronutrients. Results of this study indicate the effect of micronutrients on the suppression of nitrate accumulation was relatively small in comparison to the contents of nitrate in leaves of spinach and lettuce. To maximize the effect, nutrient composition in solution, application time, and frequency should be further examined, taking into consideration nitrogen level in soil and other environmental factors including light condition.

• Weed & Turfgrass Science
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• v.6 no.3
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• pp.272-281
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• 2017

This study was conducted to evaluate the effect of liquid fertilizer containing humic acid (LFHA) on changes of creeping bentgrass quality and growth. Treatments were designed as follows; control fertilizer (CF), HA-1 ($CF+1ml\;m^{-2}LFHA$ LFHA), HA-2 ($CF+2ml\;m^{-2}LFHA$ LFHA), and HA-3 ($CF+4ml\;m^{-2}LFHA$). As compared with CF, soil chemical properties and chlorophyll content of clipping of LFHA treatments were not significantly different. Visual quality in both of HA-2 and HA-3 treatments was higher than that of CF treatment from September to November, and clipping yield on October 27. Shoot density, root length, and the content of nitrogen, phosphorus or potassium were increased by application of LFHA. The clipping yield was positively correlated with phosphorus content, potassium content or shoot density. Similarly, LFHA level was proportionate to clipping yield of creeping bentgrass, and N, P, K contents in the leaf tissue. These results generally demonstrated that the application of LFHA improved the growth and quality of creeping bentgrass by increase of N content or P in leaf tissue.

• Journal of Wetlands Research
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• v.16 no.4
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• pp.443-452
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• 2014

Feces contain nutrients such as nitrogen and phosphate, potassium and research of the impact on the environment from feces is very diverse. However, despite the importance of mammals to environment, studies of mammals into the terrestrial ecosystems are rare. Korean water deer (Hydropotes inermis) is an endemic species of Korea and China, and we conducted on factor of plant growth in relation to feces of Korean water deer depending on land use patterns. The analysis of growth, biomass and C/N ratio of Zea mays, amount of feces and land use patterns are strongly affecting the C/N ratio and growth of the Zea mays. The quantity of the feces also influences the growth of plants instead of the land use patterns. Furthermore, the feces of Korean water deer have a lower C/N ratio than that of other animals' feces. Being the process of composting, it is able to serve a suitable fertilizer for a crop growth. In this study, we investigated the impact of feces into the environment in the terrestrial ecosystems and we were able to provide the basic data to the resources of an efficient scheme of animal feces. Further research is needed to quantify the materials amongst the plant, soil and animal, and to understand the relationship ecosystem.

• Korean Journal of Environmental Agriculture
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• v.26 no.4
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• pp.313-318
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• 2007

In greenhouse farming, a variety of humic acids have been applied to improve soil conditions and plant growth. However, it is still unclear that how humic acids combined with chemical fertilizers affect growth and quality of fruit vegetable crops. This study was conducted to determine the combination effect of humic acids and chemical fertilizers on the growth and fruit quality of tomato (Lycopersicon esculentum MILL.) grown under greenhouse conditions. Three different formulation types of humic acid were used: liquid type A, liquid type B and solid type C. The tomato plants were grown in three treatment combination plots and in conventional fertilizer (CF) plot with recommended levels of nitrogen, phosphorus and potassium: HA combined with CF (HA+CF), HB combined with CF (HB+CF) and HC combined with CF (HC+CF). For most of growth characteristics (i.e. leaf number, internode length, maximum leaf length, leaf width and chlorophyll contents) determined in this experiment, no significant differences were observed between all combination treatments and CF. However, integrated fruit qualities (i.e. averaged weight, sugar contents and acidity) were slightly improved in the humic acid combined with CF treatments when compared with CF alone treatment. No phytotoxicity was observed with humic acid treatments. However, further studies will probably be needed to use widely and safely these humic acids, in order to ensure a maximizing growth, fruit yield and quality of tomato.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.15-22
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• 2002

This study was conducted from April 13 to October 8, 2001 in Jeju to determine the influence of cutting height (2, 4, 6, 8 and 10 cm from the soil surface) on growth, forage yield and chemical composition of kenaf (Hibiscus cannabinus L.). As cutting height was increased from 2 to 10 cm, plant height averaged across two cuttings increased from 157.7 to 184.7 cm. This pattern held fir the number of leaves and branches per plant, stem diameter and plant weight per plant. As cutting height increased from 2 to 10 cm, fresh forage, dry matter, crude protein and TDN yields increased from 85.5 to 113.7MT/ha, from 11.97 to 15.63 MT/ha, from 1.63 to 2.72, and from 4.95 to 7.54 MT/ha, respectively. As cutting height was increased from 2 cm to 10 cm, crude protein, ether extract, nitrogen free extract, and TDN contents increased from 14.2 to 17.6%, 2.9 to 3.9%, 24.2 to 25.8% and 43.1 to 48.5%, respectively, while crude fiber and crude ash contents decreased from 35.5 to 30.4 % and 9.9 to 8.1 %, respectively.

• Korean Journal of Food Science and Technology
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• v.45 no.4
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• pp.478-487
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• 2013

Onions were treated with different amounts of sulfur (S) during cultivation and examined for their physicochemical properties and flavor. Onions cultivated without S application were the control group; the treatment groups were grown in soil that had been pretreated with S and received additional S applications or four times before harvest. As the number of S applications increased, the levels of crude protein, quantified with total nitrogen; ash, approximating total amounts of minerals; and dietary fiber of the onions tended to increase. The mineral compositions also improved, with noticeable increases in the levels of Mg, K, Fe, and Zn. In particular, the reducing capacity of the onions increased appreciably (p<0.05), without increases in the levels of S-containing compounds such as thiosulfinate or S-containing amino acids. Nevertheless, the spicy hot taste and flavor, which is generated mainly from S-containing compounds, were perceived more strongly in the onions that had received more S applications.

• Applied Biological Chemistry
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• v.47 no.4
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• pp.373-378
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• 2004

In this study, established soil-borne Bacillus sp. FF-9 with strong antifungal activity was isolated for identification and to determine optimal culture condition. By using 16s rDNA sequencing method, FF-9 of the selected bacteria was identified as genus Bacillus sp., Bacillus sp. FF-9 was cultured at $30^{\circ}C$, for 24 h in the LB medium. Cell growth increased quickly after 6 h and the highest cell growth was indicated at 12 h. The most antifungal activity against Rhizoctoina solani AGII-II appeared at 18 h and the optimal temperature and pH were 30 and pH 8.0, respectively. A testing of carbon and nitrogen sources showed the highest antifungal activity at 1% lactose and 1% yeast extract Furthermore an addition of salt showed the most antibiotic activity in the 0.15% $K_2HPO_4$.