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Isolation, Identification and Optimal Culture Condition of Bacillus sp. FF-9 Having Antifungal on the Turf Grass Pathogens Caused by Rhizoctonia solani AGII-II  

Park, Jin-Chul (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
Yoo, Ji-Hyun (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
Cha, Jae-Young (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
Kim, Min-Seok (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
Cho, Young-Su (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
Publication Information
Applied Biological Chemistry / v.47, no.4, 2004 , pp. 373-378 More about this Journal
Abstract
In this study, established soil-borne Bacillus sp. FF-9 with strong antifungal activity was isolated for identification and to determine optimal culture condition. By using 16s rDNA sequencing method, FF-9 of the selected bacteria was identified as genus Bacillus sp., Bacillus sp. FF-9 was cultured at $30^{\circ}C$, for 24 h in the LB medium. Cell growth increased quickly after 6 h and the highest cell growth was indicated at 12 h. The most antifungal activity against Rhizoctoina solani AGII-II appeared at 18 h and the optimal temperature and pH were 30 and pH 8.0, respectively. A testing of carbon and nitrogen sources showed the highest antifungal activity at 1% lactose and 1% yeast extract Furthermore an addition of salt showed the most antibiotic activity in the 0.15% $K_2HPO_4$.
Keywords
antifungal activity; Bacillus sp.; Rhizoctonia solani AGII-II;
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