• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.561-572
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• 2017

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.4
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• pp.514-522
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• 2017

Objective: The study aimed to assess the N use efficiency (NUE) of pig slurry (in comparison with chemical fertilizer) for each regrowth yield and annual herbage production and their nutritive value. Methods: Consecutive field experiments were separately performed using a single application with a full dose of N (200 kg N/ha) in 2014 and by four split applications in 2015 in different sites. The experiment consisted of three treatments: i) control plots that received no additional N, ii) chemical fertilizer-N as urea, and iii) pig-slurry-N with five replicates. Results: The effect of N fertilization on herbage yield, N recovery in herbage, residual inorganic N in soil, and crude protein were significantly positive. When comparing the NUE between the two N sources (urea and pig slurry), pig slurry was significantly less effective for the earlier two regrowth periods, as shown by lower regrowth dry matter (DM) yield, N amount recovered in herbage, and inorganic N availability in soil at the 1st and 2nd cut compared to those of urea-applied plots. However, the effect of split application of the two N sources was significantly positive at the last two regrowth periods (at the 3rd and 4th cut). The two N sources and/or split application had little or no influence on neutral detergent fiber (NDF) content, acid detergent fiber (ADF) content, and in vitro DM digestibility, whereas cutting date was a large source of variation for these variables, resulting in a significant increase in in vitro DM digestibility for the last two regrowth periods when an increase in NDF and ADF content occurred. Split application of N reduced the N loss via nitrate leaching by 36% on average for the two N sources compared to a single application. Conclusion: The pig slurry-N was utilized as efficiently as urea-N for annual herbage yield, with a significant increase in NUE especially for the latter regrowth periods.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.12
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• pp.861-867
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• 2011

In order to understand the extent of contamination of the sediments on the Mankyoung and Dongjin Rivers flowing into the Saemangeum Reservoir, the concentrations of ignition loss (IL), COD, total nitrogen (T-N), total phosphorus (T-P), and heavy metals (As, Cd, Cr, Pb, Zn) were measured. The average concentrations of IL, COD, and T-P were 2.40 (${\pm}2.18$)%, 2.97 (${\pm}3.06$) mg/g sediment, 0.370 (${\pm}0.351$) mg/g sediment, respectively, showing the extent of contamination is not serious. However, the concentrations of these parameters in the samples taken in year 2009 were higher than those in year 2008, indicating the contamination of sediments goes on gradually. The concentrations of T-N were in the range of 0.59~13.11 mg/g sediment with variation of locations and seasons. It was determined that the T-N contamination is serious when the concentrations were compared with the dredging guidelines of sediments in the Korean freshwaters, indicating the countermeasures are required to maintain the water quality of the Saemangeum Reservoir. The concentrations of heavy metals except chromium did not exceed the worrisome level of soil contamination stipulated by the Soil Environment Conservation Act of Korea. The high concentration of chromium to be concerned in some samples from the upstream of Mankyoung River requires source analysis and countermeasure to control the contamination.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.5
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• pp.272-279
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• 2002

A field experiment was conducted to investigate the effect of N-use efficiency and rice yield by rapeseed cake(organic fertilizer) application at panicle initiation stage in machine transplating cultivation from 1999 to 2000. The rice yield increased by 4% in "50% rapeseed cake application", by 2% in "30% rapeseed cake application" at panicle initiation stage, respectively. Amount of fertilizer N uptake was high according to increasing amount of rapeseed cake application at panicle initiation stage compared with conventional treatment, but percentage recovery of fertilizer N was higher in 30% rapeseed cake application than in 50% rapeseed cake application at panicle initiation stage. Thus, this result was thought that there was more adventageous in 30% rapeseed cake application than 50% rapeseed cake application at panicle initiation stage in the fertilizer reduction or N-use efficiency respects in rice paddy.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.403-410
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• 1996

Variations of temperature and physicochemical environment during composting of a mixtures(2:1, v/v) of cattle manure and rice hulls(CMR) in two different composting methods, static windrow(SW) and aerated static pile system(ASPS), were monitored for evaluating the efficient composting system in greenhouse. The pH of composting materials increased to around 8.9 initially, then decreased and stabilized slowly to the neutral value. Composting materials in ASPS showed a rapid stabilization in pH value from the 4th week comparing to the speed in SW. Thermophilic stage for ASPS Lasted at 3 week whereas 6 weeks for WS. Required time to get thermophilic zone in compost was shorter in ASPS than in WS. Reduction rate in total carbon(T-C) was higher in ASPS than in WS. Organic matter was reduced more rapidly in ASPS than in SW showing 9 percent difference after the 6th week. Total nitrogen(T-N) increased while composting process, showing 9 percent after 6th week in WS and 1.8 percent after 7th week in ASPS. C/N ratio was stabilized after 6th week showing 17 and 21 level in WS and ASPS each. Quantity of ash and mineral content increased during composting in both system, showing higher content in ASPS. Composting process by intermittent, aerated static pile system in greenhouse had a significant effect on the reduction of required period for composting.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.1
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• pp.31-36
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• 1993

A pot experiment was carried out to investigate the influence of application of nitrogen, phosphorus and calcium on the yield and uptake of Sr-90 by soybean plant. The results obtained were summarized as follows : 1. The application of N tended to decrease the dry matter yield of soybean. There was, however, difference in the effect on the yield between $NH_4-N$ and $NO_3-N$ ; the yield was higher in the former in the latter. On the other hand, the application of P and Ca increased the yield. 2. The uptake of Sr was decreased by the application of N, but such effect was greater in $NH_4-N$ than in $NO_3-N$. 3. The effect of N application in reducing the uptake of Sr. was enhanced by the application of P with N. 4. The combined application of P and lime showed significant effect of reducing the uptake of Sr by soybean plant. The uptake of Sr was lowest in the pot that received P, lime and $NH_4-N$ together.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.206-214
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• 2011

Fifteen clones of poplars, 2 clones of willows, and yellow poplar were used to evaluate the effects of 5 treatments such as SCBLF (slurry composting and biofiltration liquid fertilizer), general slurry liquid fertilizer, chemical fertilizer, groundwater, and control (no treatment) on vitality, growth performance, and biomass production. Five cuttings for each tree species were planted in 3 replications. After planting cuttings, a coppice was induced by cutting off stems at 10cm above the ground. Data were collected for first growing season and trees were harvested at the end of October. Maximum mortality rate i.e. 96% was recorded in the cuttings treated with groundwater and minimum 92% with control (no treatment). In all tree species, sprouting of stump was not differ significantly among the treatments. Total nitrogen concentrations of leaves and stump sprouts were higher in the treatment of SCBLF than the control, 26.6% and 22.9%, respectively. Biomass production was highest in the stumps treated with chemical fertilizer, $1.98Mg\;ha^{-1}\;year^{-1}$, and lowest in control ($1.34Mg\;ha^{-1}\;year^{-1}$).

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.347-354
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• 2015

Soybean is well known to be originated from Korea and far-east Asian countries, and studies of many root nodule bacteria associated with soybean have mainly-focused on nitrogen fixation, but much less study was carried out on bacterial community in the rhizosphere of soybean. In this study, we analyzed the bacterial community in rhizosphere of Korean soybean, Daepungkong using the pyrosequencing method based on the 16S rRNA gene to characterize the change of the rhizosphere community structure according to the growth stages of soybeans and to elucidate bacterial core community in rhizosphere of soybean. Our results revealed that bacterial community of rhizosphere soil differed from that of bulk soil and was composed of a total of 21 bacterial phyla. The predominant phylum in the rhizosphere of soybean was Proteobacteria (36.6-42.5%) and followed by Acidobacteria (8.6-9.4%), Bacteroidetes (6.1-10.9%), Actinobacteria (6.4-9.8%), and Firmicutes (5.7-6.3%). The bacterial core community in soybean rhizosphere was mainly composed of the operational taxonomic units (OTUs) belonging to the phylum Proteobacteria throughout all growth stages. The OTU00006 belonged to the genus Bradyrhizobium had the highest abundance and Steroidobacter, Streptomyces, Devosia were followed. These results show that bacterial core community in soybean rhizosphere was mainly composed of OTUs associated with plant growth promotion and nutrient cycles.

• Korean Journal of Agricultural Science
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• v.20 no.2
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• pp.145-152
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• 1993

Vegetational data obtained from 15 quadrats of Mt. Daeam area were analysed by applying two multivariate methods : two-way indicator species analysis(TWINSPAN) for classification and detrended canonical correspondence analysis(DCCA) for ordination. The dominant tree species were Quercus mongolica, Acer pseudo-sieboldianum, Betula costata, and Acer mono in descending order. The pH ranges were 5.0 to 5.5 in the 15 sites near bog and 5.3 to 5.4 in the 3 sites within bog. The forest communities in Mt. Daeam were classified into Quercus mongolica-Acer mono, Quercus mongolica-Prunus sargentii, Quercus mongolica-Abies nephrolepis-Acer tschonoskii var, rubripes, and Quercus mongolica-Abies nephrolepis-Betula costata according to the TWINSPAN. The relationships between the distribution of dominant species of forest vegetation and soil in the forest of Mt. Daeam were investigated by analyzing soil nutrition gradient. Quercus mongolica-Prunus sargentii forests were distributed in the good sites in nutrition levels of total nitrogen, cation exchange capacity, $Mg^{+{+}}$ and $Ca^{+{+}}$ but Quercus mongolica-Abies nephrolepis-Betula costata forests in the poor sites in the nutrition levels.

• Journal of the Korean earth science society
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• v.22 no.6
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• pp.480-490
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• 2001

The soil-air exchange processes of Hg were investigated from the Hari area of Kang Hwa Island during the late March 2001. In the course of our study, we determined the concentration gradients of Hg and combined these gradient data with micrometeorological components to derive its fluxes. Results of our study indicate that the concentration levels of Hg in the study area are notably lower than those typically found in urbanized areas of Korea. However, the computed fluxes were seen to be significantly larger for relatively remote areas, reaching over 200 ng/m$^2$/hr. Comparison of environmental conditions shows that the concentrations of most pollutant species including NO$_X$ and PM were significantly higher during emission, while meteorological conditions were characterized by high temperature and low humidity. Results of correlation analysis also indicate that such pollutants as hydrocarbons, nitrogen oxides, and PM generally exhibit strong positive correlations with Hg-related parameters during emission events, while such relationships were reversed during dry deposition events. The results of our present study suggest the possibility that the concentrations and fluxes of total gaseous Hg observed during deposition events can be influenced by the processes that are also important for the fine, rather than coarse, size fraction of particles.