• Journal of Applied Biological Chemistry
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• 제63권2호
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• pp.125-129
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• 2020

Environmental pollution caused by various heavy metals is a serious global problem. To solve this problem, microbial bioremediation of contaminated metals has developed rapidly as an effective strategy when physical and chemical techniques are not suitable. In this study, cadmium (Cd)-tolerant soil bacteria were isolated via artificial induction in laboratory conditions instead of screening bacteria naturally adapted to metal-contaminated soils. Wild-type (WT) bacteria grown in uncontaminated soils were artificially and sequentially adapted to gradually increasing Cd concentrations of up to 15 mM. The resultant cells, named Soil-CdR15, survived at a Cd concentration of 10 mM, whereas WT cells failed to survive with 4 mM Cd on solid media for 2 d. In liquid media containing Cd, the SoilCdR15 cells grew with 15 mM Cd for 7 d, whereas the WT cells could not grow with 5 mM Cd. Both Soil-CdR15 and WT cells removed approximately 35% of Cd at the same capacity from liquid media containing either 0.5 or 1.0 mM Cd over 2 d. In addition to Cd, the Soil-CdR15 cells showed increased resistance to nickel, zinc, and arsenic compared to WT cells. The Soil-CdR cells were identified as Burkholderia sp. by partial sequencing of 16S rRNA. The data presented in this study demonstrate that isolation of heavy metal-tolerant microorganisms via artificial induction in laboratory conditions is possible and may be useful for the application of the microorganisms for the bioremediation of heavy metals.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2002년도 추계학술발표회
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• pp.283-286
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• 2002

Solubilization and partitioning of naphthalene was investigated in an aqueous system containing soils and surfactants. The environmental behavior of polycyclic aromatic hydrocarbons(PAHs) was mainly governed by their solubility and partitioning properties on soil media in a subsurface system. In surfactant-enhanced remediation systems, surfactants might be an additional variable. a natural soil ,silica and kaolinite were tested as soil media. two nonionic surfactants, Triton X-100 and Hydropropy1-$\beta$-cyclodextrin (HPCD) were employed for naphthalene solubilization. Naphthalene showed linear on natural soil while non-linear sorption on silica and kaolinite. Soils have higher sorption capacity for Triton X-100 than HPCD indicating Triton X-100 formed ad-micelle on the soil surface. Desorption study showed a hysterysis and reversible desorption. The partitioning coefficient(K$_{D}$) of naphthalene was increased as the concentration of surfactant was increased. (below CMC), however, the coefficient was decreased above CMC. This indicates that naphthalene is partitioned into the micelles and the partition occurs competitively on both ad-micelle and free micelles as surfactant concentration increases. Therefore, the target compounds to be dissolved into aqueous phase in a surfactant enhanced remediation system might be highly partitioned on to the ad-micelle resulting in an adverse effect rather increased solubilization would be achieved.d.

• Weed & Turfgrass Science
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• 제3권3호
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• pp.240-245
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• 2014

This study was carried out to determine the possible use of volcano eruption sand at Merapi mountain area compared with clayey soil, alfisol as turfgrass growing media. Indonesia has abundant source of eruption sand and clayey soil. Native zoysiagrass (Z. matrella) was collected from Sleman district and seashore paspalum (P. vaginatum) from Solo region. The experimental plots were treated with both organic and inorganic fertilizers. Zoysiagrass and seashore paspalum can grow on the mixture of eruption volcano sand and alfisol soil. Fertilizers application increased turf color, surface coverage rate and recovery rate of turfgrass. While fertilizer treated alfisol have no effect on the length and dry weight of zoysiagrass root. Mixing of volcano eruption sand on growing media resulted in longer root length, higher shoot dry weight of zoysiagrass than in alfisol soil. Type of fertilizer affected the pH, soil organic matter, total N, available P, available K and electrical conductivity of sand and alfisol after cultivation. Similarly to zoysiagrass, the recovery of seashore paspalum was increased by application of fertilizer, even the inorganic fertilizer showed faster recovery than organic fertilizer. Surface coverage of seashore paspalum was faster than that of zoysiagrass with or without fertilizer.

• 한국환경농학회지
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• 제19권3호
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• pp.234-241
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• 2000

슬러지를 토양개량제로 재활용하기 위하여 슬러지 처리에 요업소성 공법을 적용하여 다공극성의 인공배지를 생산하였다. 슬러지를 이용해 인공배지를 생산하기 위하여 자바사이트와 석회를 첨가재로 사용하였으며, 첨가재와 슬러지를 혼합한 시료를 $800{\sim}1,100^{\circ}C$의 고온으로 열처리하였다. 비교 밭 토양에 인공배지를 혼합한 후, 혼합토양의 물리${\cdot}$화학성을 분석하였고, 작물 생육 시험을 통하여 인공배지 혼합에 따른 영향을 조사${\cdot}$분석하였다. 인공배지의 pH는 비교 밭 토양구보다 높았다. 작물 생장 시험 후 비교 밭 토양 보다 인공배지 처리구에서 대부분 CEC, OM, TN, TP 및 $AV.P_2O_5$가 높게 나타났다. 작물 생장 시험 결과 인공배지 처리구에서 콩과 옥수수의 생육 상태가 비교 밭 토양구에서 보다 좋은 것으로 나타났다. 또한 콩과 옥수수의 수확량도 각각 약 46kg/10a, 194kg/10a 정도 인공배지 처리구에서 높게 나타났다. 인공배지 처리구의 중금속 함량은 모든 처리구에서 기준치보다 낮게 나타났다. 따라서 슬러지로 만든 인공배지를 일반 밭 토양에 혼합하여 작물을 재배하여도 문제가 없을 것으로 판단된다.

• 한국농공학회:학술대회논문집
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• 한국농공학회 1998년도 학술발표회 발표논문집
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• pp.490-499
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• 1998

This study was initiated to investigate the applicability of sludge from water or waste water treatment on the crop cultivation. Sludge is generated in the process of water and wastewater treatment process in large quantity. The sludge can cause many environmental problems. we have a many available treatment methods of sludge However, these methods still shortcomings and are not. The composition of typical municipal sludge contains organics and inorganics. The organics components are normally burnt in high temperature and mainly inorganics components are left after thermal treatment process. For the production of artificial media, chabazite was used as additive, and the mixed material was thermally treated in the firing kiln at 800~1,10$0^{\circ}C$ for about 10 minutes. The physical and chemical characteristics of artificial media were analyzed and it showed that the artificial media could be used as a media for plants and soil conditioner for farmland. The concentrations of the toxic heavy metals in the media were lower then those in the soil quality standard for farmland. This study illustrated that the artificial media production process, and introduced how to produce it's possible application as a media for Plant growth.

• 한국토양비료학회지
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• 제47권6호
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• pp.510-517
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• 2014

The purpose of this study was to analyze microbial hazards for cultivation environments and personal hygiene of strawberry and tomato farms at the growth and harvesting stage. Samples were collected from thirty strawberry farms and forty tomato farms located in Korea and tested for Staphylococcus aureus and Bacillus cereus. To investigate the change in the distribution of the S. aureus and B. cereus, a total of 4,284 samples including air born, soil or medium, mulching film, harvest basket, groves and irrigation water etc. were collected from eight strawberry farms and nine tomato farms for one year. As a result, total S. aureus and B. cereus in all samples were detected. Among the total bacteria of strawberry farms, S. aureus (glove: $0{\sim}2.1Log\;CFU/100cm^2$, harvest basket: $0{\sim}3.0Log\;CFU/100cm^2$, soil or culture media: 0~4.1 Log CFU/g, mulching film: $0{\sim}3.8Log\;CFU/100cm^2$), B. cereus (glove: $0{\sim}2.8Log\;CFU/100cm^2$, harvest basket: $0{\sim}4.8Log\;CFU/100cm^2$, soil or culture media: 0~5.3 Log CFU/g, mulching film: $0{\sim}4.5Log\;CFU/100cm^2$) were detected in all samples. The total bacteria of tomato farms, S. aureus (glove: $0{\sim}4.0Log\;CFU/100cm^2$, harvest basket: $0{\sim}5.0Log\;CFU/100cm^2$, soil or culture media: 0~6.1 Log CFU/g, mulching film: $0{\sim}4.0Log\;CFU/100cm^2$), B. cereus (glove: $0{\sim}4.0Log\;CFU/100cm^2$, harvest basket: $0{\sim}4.3Log\;CFU/100cm^2$, soil or culture media: 0~5.9 Log CFU/g, mulching film: $0{\sim}4.7Log\;CFU/100cm^2$) were detected in all samples. The contamination of S. aureus and B. cereus were detected in soil, mulching film and harvest basket from planting until harvest to processing, with the highest count recorded from the soil. But S. aureus and B. cereus were not detected in irrigation water samples. The incidence of S. aureus and B. cereus in hydroponics culture farm were less than those in soil culture. The amount of S. aureus and B. cereus detected in strawberry and tomato farms were less than the minimum amount required to produce a toxin that induces food poisoning. In this way, the degree of contamination of food poisoning bacteria was lower in the production environment of the Korea strawberry and tomato, but problems can be caused by post-harvest management method. These results will be used as fundamental data to create a manual for sanitary agricultural environment management, and post-harvest management should be performed to reduce the contamination of hazardous microorganisms.

• 한국환경과학회지
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• 제22권7호
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• pp.801-810
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• 2013

To improve the physical and chemical properties of the soil and increasing water-retaining property of the soil, Superabsorbent synthetic polymeric materials have been used. The experiment carried out from April to July 2012 after the influence evaluation of Superabsorbent synthetic polymeric materials to vines plant. The result shows that the study of Hedera japonica Tobler, the growth and the survival rates rank as media > hydroponic > superabsorbent synthetic polymers, and the growth and the survival rates are considerably pessimistic in the experiment of hydroponic and superabsorbent synthetic polymers. In the study of Trachelospermum asiaticum var. intermedium, the growth and the survival rates rank as hydroponic > media > superabsorbent synthetic polymers, the difference between the experiment of hydroponic and is very small and the survival rates are not very good in the experiment of superabsorbent synthetic polymers. In the study of Euonymus fortunei var. radicans Rehder,it is insignificantly difference among the different of planting based. Judging from these results, the differences are depending on the species of plants. The thesis holds that the characteristics of plant should be considered in plant cultivation and soil improvement in the future, and it is desirable to use the appropriate mixing ratio of soil in soil improvement as well.

• 한국토양비료학회지
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• 제44권3호
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• pp.415-424
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• 2011

This research was conducted to verified the effectiveness of a sintered porous media coated with organic matter as nutrient source and microorganisms as decomposer effective in TPH decomposition for a closed-typed biopole system. The organic matter content in the sintered porous media which was developed with bentonite increased with increasing dilution ratio of pig slurry and the sintered porous media as well as decrease in the particle size of sintered porous media. The decomposition rate of TPH was significantly increased with increasing aeration than that under atmospheric condition. Also the sintered porous media containing organic matter and microorganisms proved that the decomposition was enhanced with addition of nutrients sources in addition to aeration periodically.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.252-254
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• 2004

In the past several years phytoremediation, defined as the use of plants for removing contaminants from media such as soils or water, has attracted a great deal of interest as a potentially useful remediation technology. In this study, we have attempted to asses the effectiveness of phytoremeidation of disel contaminate soils using hybrid poplar species. 3 poplar species had removed disel from soil effectively and toxic effect was also observed over 2500mg/kg disel contaminated soil, which indicating reducing disel removal.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2001년도 총회 및 춘계학술발표회
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• pp.132-135
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• 2001

To understand and compare tracer transport in fractured and porous media. multiple tracer tests were conducted in Wonju and Uiwang sites. The target media were fractured in Wonju site and porous in Uiwang site. It was known that groundwater flow for the two hydrogeologic systems could be represented using a EPM approach. However, the tracer transport in the two aquifer systems was greatly different. In this study, we analyzed the different tracer transport behavior in the two systems, from which our understanding of the tracer dispersion was greatly enhanced. we used bromide and chloride as tracers.