• Journal of Soil and Groundwater Environment
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• v.11 no.5
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• pp.43-50
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• 2006

Bioreporter bacteria, such as recombinant bioluminescent bacteria, have been used for the detection of specific compounds in complex environmental media. In this study, optimum conditions for the preparation and application of deep-freezed and Iyophilized recombinant bioluminescent strain KG1206 were investigated for the future application on contaminated environmental sites. Genetically engineered microorganism, Pseudomonas putida mt-2 KG1206, contains TOL plasmid and the plasmid inserted $P_{m}$, promoter on the upper part of lux gone in vector pUCD615, and m-toluate and benzoate are considered direct inducers for bioluminescence. Optimum conditions determined for the preparation and application of the deep-freezed and lyophilized strain were followings: cryoprotective agent (24% sucrose), lyophilization time (12 hrs), strain concentration ($OD_{600}=0.6$), reconstitution for freezed strain (quick reconstitution at $35^{\circ}C$), reconstitution for lyophilized strain ($3{\sim}6$ hrs exposure on LB medium), carrying conditions (keep at $20^{\circ}C$ after reconstitution). These results demonstrate the feasibility of deep-freezed or lyophilized state of genetically engineered bioluminescent strain for environmental usage.

• Journal of The Geomorphological Association of Korea
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• v.19 no.3
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• pp.23-36
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• 2012

This study aims to think about the problem of dolmens: the reasons for the presence of dolmens and how to construct it, at the point of view of geographical and geomorphological. The subjects of this study is the dolmens which locate between at Dogok Hyosanri and Chunyang Daesinri Hwasoon-gun, Jeonlanamdo. The study areas in Hyosanri, Daesinri has been observed as follows. First, the long axis direction of Dolmen upper stone and the slope one are the same. Second, tor, block stream, hockey stick, etc. Third, Composition of the soil silt> sand> clay is distributed in the order. Forth, The soil of high quality silt and the roundness of angular, sub-angular-level and the high frequency, peaks of quarts and illite clay minerals show. Fifth, in the result of $SiO_2/Al_2O_3$, $SiO_2/R_2O_3$, and CIA(Chemical Index of Alteration), Hyosanri, Daesinri areas show mechanical weathering was dominant and chemical weathering environment was not being progressed. The blocks used in construction of dolmens had moved to the bottom of slopes by mass movement such as solifluction then them which had been placed in the position seem to be used by people of Bronze. Based on the above results, the process of construction of dolmens can be estimated as follows. They would dig up the ground under the upper stone of dolmens, put the supporting stone in the place, then dig up earth, place into remains, close the obturating stones, then heal up earth.

• Journal of Conservation Science
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• v.36 no.6
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• pp.494-504
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• 2020

Ten historic sites (denoted as A- J in this study) of a tomb were found during the construction of the east-west expressway in District 2 of Hyangnam, Hwaseong, which is implemented by the Gyeonggi-do headquarters of the Korea Land & Housing Corporation. Thetombswere first detected at siteH, and further investigations revealed various tombs from the Three Kingdoms period; artifacts such as gilt-bronze shoes and caps were excavated from wooden coffins in the tombs. The pottery examined in this study was the only pottery artifact excavated from the site. Its raw clay was soft and loose, reddish brown, and had quartz and feldspar particles of < 1 mm, which appeared to have been added as reinforcing agents. The firing temperature of the pottery was estimated to be under 800-870 ℃ as the mica remains and tridimite, which is the phase transition mineral of quartz, was not produced; a slight endothermic peak was also detected because of the hydration of sericite at 800 ℃. The condition of the artifact was severely weakened because of various factors, such as soil pressure from the stratum formed over the site and repeated freezing and thawing. The artifact could not be collected alone, and thus, surrounding soil that had attached to the artifact was also collected; the artifact was transported to the laboratory and conservation treatment was conducted in a safe and systematic manner.

• Journal of the Korean Geotechnical Society
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• v.40 no.3
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• pp.93-100
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• 2024

Pore water freezes in low-temperature compaction, which leads to different compaction characteristics compared to room temperature conditions. In regions like Alberta, Canada, where organic soils are prevalent, compaction performance is influenced by the high water retention and compressibility of organic soils, as well as their sensitivity to freezing and thawing. Alberta's strict environmental regulations demand the reuse of excavated soil for backfill, and the long winter season creates challenging conditions for civil engineering projects. In this study, a laboratory compaction test was conducted to evaluate the low-temperature compaction characteristics of organic soils with varying organic content. The results indicate that the optimum moisture content increases as the organic content increases, and the maximum dry unit weight decreases by up to 21.9%. In addition, under temperature conditions below -4℃, no optimum moisture content was observed, and the dry unit weight decreased as the moisture content increased.

• Korean journal of applied entomology
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• v.61 no.3
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• pp.409-422
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• 2022

Two dominant thrips in hot pepper (Capsicum annuum) cultivating in greenhouses are Frankliniella occidentalis and F. intonsa in Korea. This study investigated their overwintering physiology. These two thrips were freeze-susceptible and suppressed the body freezing temperature by lowering supercooling point (SCP) down to -15~-27℃. However, these SCPs varied among species and developmental stages. SCPs of F. occidentalis were -25.7±0.5℃ for adults, -17.2±0.3℃ for pupae, and -15.0±0.4℃ for larvae. SCPs of F. intonsa were -24.0±1.0℃ for adults, -27.0±0.5℃ for pupae, -17.2±0.8℃ for larvae. Cold injuries of both species occurred at low temperature treatments above SCPs. Thrips mortality increased as the treatment temperature decreased and its exposure period increased. F. occidentalis exhibited higher cold tolerance than F. intonsa. In both species, adults were more cold-tolerant than larvae. Two thrips species exhibited a rapid cold hardening because a pre-exposure to 0℃ for 2 h significantly enhanced the cold tolerance to a lethal cold temperature treatment at -10℃ for 2 h. In addition, a sequential exposure of the thrips to decreasing temperatures made them to be acclimated to low temperatures. To investigate the overwintering sites of the two species, winter monitoring of the thrips was performed at the greenhouses. During winter season (November~February), adults of the two species were not captured in outside of the greenhouses. However, F. occidentalis adults were captured to the traps and observed in weeds within the greenhouses. F. occidentalis adults were also emerged from soil samples obtained from the greenhouses during the winter season. F. intonsa adults did not come out from the soil samples at November and December, but emerged from the soil samples obtained after January. To determine the adult emergence due to diapause development, two thrips species were reared under different photoperiods. Adult development occurred in all photoperiod treatments in F. occidentalis, but did not in F. intonsa especially under short periods. Tomato spotted wilt virus, which is transmitted by these two species, was detected in the weeds infested by the thrips during the winter season. These results suggest that F. occidentalis develops on weeds in the greenhouses while F. intonsa undergoes a diapause in the soil during winter.

• Journal of the Korean Geosynthetics Society
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• v.15 no.3
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• pp.57-66
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• 2016

In this study, to prevent possible collapse caused by hydraulic or mechanical instability, liquid nitrogen injection method is developed and implemented at the tip of drilling auger of steel pipe propulsion tunneling. In this study, 1/5-scale model auger and sand chamber were manufactured. The prototype diameter of steel pile (or casing) is assumed about 1,000 mm. For the frictional sandy soils and plastic weathered soils, liquid nitrogen injection methods were tested varying water contents of the soils. For the induced hydraulic instability, the ground near the drilling auger was frozen within approximately 5 minutes preventing mechanical collapse and water infiltration. Securing stability of steel pile propulsion tunneling using liquid nitrogen was much more effective for which the water content of the soil somewhat exceeds the optimum water content.

• Journal of the Korean GEO-environmental Society
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• v.8 no.6
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• pp.53-60
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• 2007

L/EPS, manufactured in the shape of block and used for civil engineering, is a lightweight material with an excellent resistance to compression, and provides a superb self-sufficient stability. EPS is a suitable material capable of resolving the problem of settlement and lateral flow if it is applied as the soil on soft ground. The Korean Standards (KS) has not yet proposed any testing method for use of EPS as an engineering banking material. Only its testing and quality ordinance as a heat insulation material has been standardized. The design criteria for EPS has been established and applied through the trial construction of KHC (Korea Highway Corporation) and quality test of manufacturer, but most studies on them have been confined to factory products. This study is focused on comparing and analyzing long-term durability by conducting cyclic load test, freezing and thawing test, absorption rate test and others. EPS used in the test was chosen from construction sites and factory products, focusing on the long-term durability of EPS depending on the passage of time. Unconfined compression test results indicated that the strength of collected samples was lower than factory products. While the triaxial compression test results indicated that the shear strength increased in proportion to the increase of confining pressure, and factory products had declining shear strength as the confining pressure rose.

• Journal of Mushroom
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• v.15 no.2
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• pp.69-72
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• 2017

Diversification to develop oak pruned neck utilization technology reduction of operation cost of cheonma(Gastrodia elata) farmers is the purpose of this research, but the results of the experiment are as follows. The soil chemistry of the plantation was very weak in 2015 when the pH was 5.7, weakly acidic and EC was 0.41 (ds/m), but the faux spring area was very sunny, but at the end of May, early June, late July And so on were at a maximum temperature of $25^{\circ}C$ or more, and underground temperature fell below freezing in late January 2016. In the content of the survey of the harvested volume, treatment with a diameter of 6 to 10 cm appeared in 1,366 g total weight and in treated plots with a diameter of 20 cm or more, appeared in 1,542 g, confirming that the number and weight of the interspaces from the small neck is higher than the practice.

• Microbiology and Biotechnology Letters
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• v.7 no.3
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• pp.135-140
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• 1979

A Streptomyces sp. strain AS-727 which was capable of producing penicillinase, was isolated from soil. The enzyme production was affected by the carbon and nitrogen sources added. Among them so far tested, glucose (or maltose) and sodium nitrate increased the enzyme production. And the amount of enzyme prodced reached maximum in 4 days cultivation. The optimla pH and temperature of the penicillinase was between pH 6.0 to 8.0 and 4$0^{\circ}C$ respectively. The stabel pH range of the enzyme was stable at 4$0^{\circ}C$, but it lost about 30％ and 40％ of the the activity respectively when it was treated at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 60 minutes. The activity of the enzyme was inhibited by Z $n^{++}$, but A $g^{+}$, $Co^{++}$, $_Mn^{++}$, $Ca^{++}$, P $b^{++}$ did not affected enzyme activity. Peculiarly, the enzyme protein precipitated by freezing or addition of ammonium sulfate, urea, sodium chloride and some organic solvents as etanol, methanol, acetone was not dissolved in deionized water or any buffer solution.n.n.ion.n.n.

• Journal of the Korean GEO-environmental Society
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• v.18 no.8
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• pp.23-32
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• 2017

For exposed slopes, the weathering degree over time has a major effect on the engineering properties of rocks and the slope stability. Rocks are gradually changed by weathering into soil over time, and the resulting physical, chemical and mechanical changes of rocks affect the engineering stability of the slope. However, there are not many ways to objectively evaluate the weathering degree of a slope. In this study, therefore, to investigate the weathering characteristics of rocks, granite, gneiss and shale distributed in the Chungbuk region were sampled by weathering stage and changes in their component minerals and tissues were investigated. Furthermore, artificial weathering was induced using the freezing and thawing test and quantitatively investigated through porosity and absorption rate. In addition, the changes of microcracks due to artificial weathering were evaluated through box fractal dimension ($D_B$). Through mineralogical study the phase change of constituting minerals, the growth of secondary minerals, the development of micro-cracks and the fabric changes due to weathering were observed. The mineralogical, chemical and engineering evaluations of the weathering degree through the experimental results in this study are expected to be useful for analyzing the weathering characteristics and causes by rock type and for proposing a methodology to evaluate the degradation of physical properties comparatively and quantitatively.