• Journal of Microbiology and Biotechnology
• /
• v.19 no.4
• /
• pp.339-345
• /
• 2009

We evaluated the activity and abundance of the crude-oil-degrading bacterium Nocardia sp. H17-1 during bioremediation of oil-contaminated soil, using real-time PCR. The total petroleum hydrocarbon(TPH) degradation rate constants(k) of the soils treated with and without H17-1 were $0.103\;d^{-1}$ and $0.028\;d^{-1}$ respectively. The degradation rate constant was 3.6 times higher in the soil with H17-1 than in the soil without H17-1. In order to detect and quantify the Nocardia sp. H17-1 in soil samples, we quantified the genes encoding 16S ribosomal RNA(16S rRNA), alkane monooxygenase(alkB4), and catechol 2,3-dioxygenase(23CAT) with real-time PCR using SYBR green. The amounts of H17-1 16S rRNA and alkB4 detected increased rapidly up to 1,000-folds for the first 10 days, and then continued to increase only slightly or leveled off. However, the abundance of the 23CAT gene detected in H17-1-treated soil, where H17-1 had neither the 23CAT gene for the degradation of aromatic hydrocarbons nor the catechol 2,3-dioxygenase activity, did not differ significantly from that of the untreated soil($\alpha$=0.05,p>0.22). These results indicated that H17-1 is a potential candidate for the bioaugmentation of alkane-contaminated soil. Overall, we evaluated the abundance and metabolic activity of the bioremediation strain H17-1 using real-time PCR, independent of cultivation.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.10 no.1
• /
• pp.37-45
• /
• 1990

An analytical procedure is proposed for the seismic analysis of a soil-structure interaction system with besemat uplift, including the effects of concurrent vertical seismic ground motion, nonlinear distribution of bearing soil pressure under the basemat, and 3-dimensional behavior of the system. The soil-structure interaction system is assumed to have rectangular-shaped basemat on elastic half-space. Nonlinearity of soil spring constants and soil damping coefficients induced by the base mat uplift is modeled by considering not only the reduction of contact area between soil and structure but also the effects of rigid body rotational motion of the superstructure, and the shift in the point of action of the resultant reaction on the basemat. Throught various parametric studies. it has been confirmed that the seismic responses of the superstructure reduce notably while response at the basemat increases considerably. The results also show that the effects of concurrent vertical ground motion. nonlinear soil pressure distribution under basemat, and 3-dimensional behavior of the system shall be included in uplift analysis in order to obtain the correct structural responses.

• 한국환경농학회:학술대회논문집
• /
• 2011.07a
• /
• pp.207-222
• /
• 2011

Relationships between soil saturated hydraulic conductivity ($K_s$) and porosity (${\phi}$) have been developed over many years; however, use of these relationships for evaluating rain-induced seals is limited mainly because of difficulties in estimating seal pore-size characteristics. The objectives of this study were to evaluate the $K_s$ of soil surface seals over a range of thicknesses, where seal thickness was determined using a High-Resolution-Computed-Tomography (HRCT) scanner, and to investigate relationships between $K_s$ and ${\phi}$ of developing seals in samples with equivalent diameters (e.d.) ${\geq}15\;{\mu}m$. A Mexico silt loam soil was packed to a bulk density (${\rho}_b$) of $1.1\;Mg\;m^{-3}$ in cylinders 160-mm i.d. by 160-mm long and subjected to $61-mm\;h^{-1}$ simulated rainfall having a kinetic energy (KE) of $25\;J\;m^{-2}\;min^{-1}$ for 7.5, 15, 30, and 60 min to create a range in seal development. Thicknesses of the seal layers were determined by analysis of HRCT images of seals. The $K_s$ values of the seals were estimated using an effective $K_s$ value ($K_{s-eff}$). The $K_s-{\phi}$ relationship was described by a Kozeny and Carmen equation, $K_s=B{\phi}^n$; where B and n are empirical constants and n = 31. This approach explained 86% of the variation between $K_s$ and ${\phi}$ within the soil seals. Knowledge of surface seal information and hydraulic conductivity can provide useful information to use in management of sites prone to sealing formation.

• Geophysics and Geophysical Exploration
• /
• v.9 no.4
• /
• pp.304-315
• /
• 2006

We have examined the applicability of f-k analysis to the GPR direct wave measurement for water content to characterize vadose zone condition. When the vadose zone consists of a dry surface layer over wet substratum, we obtained f-k spectra where most of the energy is bounded by the air and dry soil velocities. In this case, dry soil velocity was successfully estimated by using high frequency data. On the other hands, when wet soil overlies dry substratum, the f-k spectra show a contrasting response where most of the energy travels with the velocity bounded by dry and wet soil velocities. In this case, the radar waves are trapped and guided within wet soil layer, exhibiting velocity dispersion. By adopting modal propagation theory, we could formulae a simple inversion code to find two layer's dielectric constants as well as layer thickness. By inverting the velocity dispersion curve obtained from f-k spectra of synthetic modeling data, we could obtain good estimates of dielectric constants of each layer as well as first layer thickness. Moreover, we could obtain more accurate results by including the higher mode data. We expect this method will be useful to get the quantitative property of real subsurface when the field condition is similar.

• Journal of Ocean Engineering and Technology
• /
• v.15 no.4
• /
• pp.86-91
• /
• 2001

In lateral ground flow, slope stability, and land slide problems, H-piles have been often used for a horizontally deforming ground to prevent the failure of mass of soil in a downward and outward movement of a slope. Here, Theoretical equations are derived to estimate the lateral force, assuming that the Mohr-coulomb's Plastic states occures in the ground just around H-piles. In this study, the mechanism of lateral force acting on passive pile that is in a row, situated in the ground undergoing plastic deformation was discussed, and its theoretical analysis was carried out considering the interval between H-piles. The solution of the theoretical equation derived from here showed resonable characteristic for constants of soil as well as for the interval, widths, and heights of H-pile.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.03a
• /
• pp.1095-1099
• /
• 2008

Critical state theory involves two state boundary surface. One is Roscoe surface and the other is Hvorslev surface. The shape of these boundary surface was changed because of several parameters : Critical state constant(M), spacing ratio (r) and critical state pore pressure coefficient($\wedge$). As these constants make difference to each model and the way of solution, they may affect the shape of state boundary surface. Specially, spacing ratio (r) is important. On this study, triaxial compression test was performed using remolded weathered mudstone soil and investigated variation of state boundary surface because of spacing ratio. In the results of prediction, critical state point was located highly and the shape of boundary surface was changed more tightly curve as decreasing spacing ratio.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2002.09a
• /
• pp.122-124
• /
• 2002

The removal capacity of zero-valent iron for Cr(Ⅵ) was evaluated using batch kinetic tests. The rate constants for zero-valent iron dramatically increased as initial Cr(Ⅵ) concentration decreased. Generally, the reaction rates of Cr(Ⅵ) with zero-valent iron were faster than that of a biotic degradation of Cr(Ⅵ), and furthermore the reaction rates were inversely proportional to the initial Cr(Ⅵ) concentrations. After certain reaction time elapsed. no further decrease of Cr(Ⅵ) was observed, indicating a loss of iron reactivity. The loss of iron reactivity was primarily due to the passivation of iron surfaces with iron-Cr precipitates, but the reactivity of iron was recovered by adding iron-reducing bacteria. Even though the addition of bacteria itself removed Cr(Ⅵ), the combination of iron-reducing bactera and oxidized iron significantly enhanced the reaction rate for Cr(Ⅵ) removal. The results from column tests also confirmed that the innoculation of iron-reducing bacteria to the column containing completely oxidized iron partially enhanced the recovery of the iron reactivity.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2006.04a
• /
• pp.123-126
• /
• 2006

Reduction kinetics and intermediates behaviour of three high explosives (HMX, RDX, and TNT) were studies in batch reactors using either nano or micro size zero valent iron(ZVI) as reducing agent. The kinetics constants normalize to the mass of iron($k_M$) or to the surface area ($k_{SA}$) were measured and compared along with the changes of intermediate concentrations of each explosive. Results showed that $k_M$ and $k_{SA}$ values neither correlated each other nor explained the behaviour of intermediates of each high explosive in the batch reactor, in which initial intermediates decreased rapidly with nano ZVI treatment whereas the intermediates accumulated and stayed longer in the micro ZVI treated reactor.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.04a
• /
• pp.120-123
• /
• 2003

Immobilization behavior of methyl tert-butyl ether (MTBE) by various cyclodextrins(CDs) was studied to investigate the feasibility of MTBE removal using cyclodexrins. Even though MTBE has relatively low hydrophobicity and higher polarity compared to other organics, it was effectively immobilized by CDs. The immobilization isotherms was shown as a type of Freundlich isotherms, and the immobilization capacity of -CDs was the largest among natural COs. The initial apparent association constant for MTBE-CD complex follows the order : gamma = beta > methyl-beta > hydroxypropyl beta > alpha. These differences of the constants are related to the size of MTBE and CDs. The size of beta-CD and gamma-CD is large to encapsulate MTBE molecule into the cavity, which that of alpha-CB is too small to encapsulate MTBE.

• Bulletin of the Korean Chemical Society
• /
• v.14 no.4
• /
• pp.453-457
• /
• 1993

Cadmium(II) complexation by a well characterized soil fulvic acid (FA) from the Okchun Metamorphic Belt were studied at pH of 6.0 in 0.1 M $NaClO_4$ using the ultrafiltration technique. The conditional stability constants thus obtained were log K= 3.90${\pm}$0.15 and 3.99${\pm}$0.12 $L{\cdot}mol^{-1}$ at fulvic acid concentrations of 101 and 226 mg${\cdot}L^{-1}$ respectively. When free cadmium ion concentration was measured directly using an ion selective electrode, log K of 4.12${\pm}$0.03 $L{\cdot}mol^{-1}$ was obtained. These results show that fulvic acid forms predominately 1 : 1 complex with $Cd^{2+}$ ions. The maximum binding ability of this polyelectrolyte material was 0.886 mmol Cd/g FA. The average gram formula weight of fulvic acid was estimated to be 1130 daltons.