• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.44-44
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• 2003

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.261-261
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• 2005

• Proceedings of the Korea Water Resources Association Conference
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• 2005.09a
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• pp.446-447
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.1011-1011
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• 2005

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.403-406
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• 2002

This experimental shows the possibility of using as biofertilizer, which convert insoluble inorganic phosphate salts to plant-usable phosphate type by immobilized microorganism with calcium alginate. In the case of culture of P. agglomerans on constant medium pH, phosphate was produced 357 mg/L after 18hrs. And in the case of culture of immobilzed P. agglomerans bead, phosphate was produced maximum 295.6 mg/L after 120 hrs. Also as using rock phosphate as insoluble phosphate salts, phosphate was respectably produced 190.3 and 195.2 mg/L after 36 hrs at free cells and immobilized cells. In our experiments, the using soils contained 23.16 g-P/kg-soil total phosphate and 3.76 g-P/kg-soil soluble phosphate. The result of 1g immobilized bead seeding, soluble phosphate was produced maximum 6.14 g-P/kg-soil phosphate and this value was increased continuously.

• KSBB Journal
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• v.25 no.3
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• pp.230-238
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• 2010

A microbial strain having high keratinase activity was isolated from the soil of poultry factories of Gyeonggi or Chungcheong-do. The isolated strain was identified as Bacillus sp. based on its morphological and biochemical characteristics. In this study, the optimal conditions for the production of keratinase by this strain were investigated. The optimal medium composition for the keratinase production was determined to be 3.5% chicken feather as carbon source, 1.0% tryptone as organic nitrogen source, 1.0% $KNO_3$ as inorganic nitrogen source and 0.05% KCl, 0.05% $KH_2PO_4$, 0.03% $K_2HPO_4$ as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH was $40^{\circ}C$ and 8.5 with shaking culture (200 rpm), respectively. The maximum keratinase production reached to 123 units/ml after 42 hr of cultivation under the optimal condition. When the hair was used as the sole carbon source, the maximum enzyme activity was 88 units/ml after 120 hr and in this case, the hair added in the medium was not degraded completely but got thinner than the control by 20%.

• KSBB Journal
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• v.32 no.3
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• pp.206-211
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• 2017

The physical method used to prevent a landslide has the risk of environmental pollution. Calcite forming bacteria (CFB) have been received increasing attention as a novel and environmental friendly strategy for the soil improvement. In this study, we selected 11 CFB strains with high calcite production. We also examined survivability and calcite productivity of the strains under various stress conditions to select strains with high resistance to various stresses. Two strains was selected by environment stress. Sphingobacterium sp. KJ-32 and Viridibacillus arenosi B-25 precipitate calcite more than other strains at pH 5 and $15^{\circ}C$ respectively. Bio-consolidated soil cakes were made using various calcium salts (calcium chloride, calcium acetate, calcium lactate, calcium gluconate) with mixed culture of 2 strains. Among them, the calcite made using calcium chloride was the largest. These observations demonstrate that this bio-consolidation technology has the potential for eco-friendly prevention of landslide and soil improvement.

• KSBB Journal
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• v.25 no.1
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• pp.73-78
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• 2010

In this study, recovering agent was produced with organic sludge and modified peat moss (MPM) in pilot plant mixer to recover oil contaminated soil, and field test of it was estimated using landfarming method. Oil contaminated soil recovering agent was thought to contain more microorganisms than raw waste sludge and was no problem to come onto the market because there were not any items of specified wastes. According to the results of TPH variation with time, it was observed the initial degradation velocity of oil with produced recovering agent was rapid up to 50% after 4 days, remarkably. Because the microorganisms in the organic sludge discharged from chemical plant already acclimated with oil, therefore, it could be estimated initial degradation velocity of recovering agent might be rapid. It was concluded that the oil contaminated soil recovering agent produced in this study have high marketability because of its two aspects on recycling of wastes and initial rapid degradation capacity.

• Journal of Applied Biological Chemistry
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• v.50 no.4
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• pp.221-226
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• 2007

The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

• Animal Systematics, Evolution and Diversity
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• v.38 no.2
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• pp.51-58
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• 2022

Four monogonont rotifers, Filinia hofmanni Koste, 1980, Lecane pusilla Harring, 1914, Mikrocodides chlaena (Gosse, 1886), and Proales fallaciosa Wulfert, 1937, were newly recorded in Korea. The genera Mikrocodides Bergendal, 1892 and Proales Gosse, 1886 were recorded for the first time in Korea. Mikrocodides chlaena and Proales fallaciosa were found from soil samples and are both soft-bodied species. Filinia hofmanni has previously been recorded mainly in Europe, and this is its first record in Asia. Lecane pusilla is the 24th lecanid rotifer recorded in Korea, and its morphological characteristics are consistent with previous research of L. pusilla. We have provided the morphological diagnoses of the four Korean specimens in this study, along with the partial sequences of mitochondrial cytochrome c oxidase subunit I(COI) gene of three species.