• Title/Summary/Keyword: Soil Reaction

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A report of 42 unrecorded actinobacterial species in Korea

  • Lee, Na-Young;Cha, Chang-Jun;Im, Wan-Taek;Kim, Seung-Bum;Seong, Chi-Nam;Bae, Jin-Woo;Jahng, Kwang Yeop;Cho, Jang-Cheon;Joh, Kiseong;Jeon, Che Ok;Yi, Hana;Lee, Soon Dong
    • Journal of Species Research
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    • v.7 no.1
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    • pp.36-49
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    • 2018
  • During a study to discover indigenous prokaryotic species in Korea in 2016, a total of 42 actinobacterial isolates were recovered from various environmental samples collected from natural cave, squid, sewage, sea water, trees, droppings of birds, freshwater, eelgrass, mud flat, sediment and soil. On the basis of a tight phylogenetic clade with the closest species and high level of 16S rRNA gene sequence similarity, it was shown that each isolate was assigned to independent and previously described bacterial species which were assigned to the phylum Actinobacteria. The following 42 species have not been reported in Korea: eight species in two genera n the order Corynebacteriales, 26 species of 16 genera in the Micrococcales, one species of one genus in the Micromonosporales, one species of one genus in the Propionibacteriales, four species of two genera in the Streptomycetales and two species of two genera in the Streptosporangiale. Cell morphology, Gram staining reaction, colony colors and features, the media and conditions of incubation, physiological and biochemical characteristics, origins of isolation and strain IDs of 42 unrecorded actinobacterial species are presented in the species description.

A report on 24 unrecorded bacterial species of Korea isolated in 2016, belonging to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria

  • Joung, Yochan;Cha, Chang-Jun;Im, Wan-Taek;Jeon, Che Ok;Joh, Kiseong;Kim, Seung-Bum;Kim, Wonyong;Lee, Soon Dong;Cho, Jang-Cheon
    • Journal of Species Research
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    • v.7 no.1
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    • pp.13-23
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    • 2018
  • In 2016, as a part of the research program 'Survey of Korean Indigenous Species', diverse environmental samples were collected from various sources of freshwater, seawater, soil, wetland, reclaimed land, sand, pine forest, plant root, ginseng field, solar saltern, and caves. Thousands of bacterial strains were isolated from the diverse samples and identified based on 16S rRNA gene sequence analyses. The present study, as a phylogenetic subset of the primary research program, reports 24 unrecorded bacterial species in Korea that belong to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria. Based on the high 16S rRNA gene sequence similarities (>98.8%) and formation of a robust phylogenetic clade with the closest type species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 24 bacterial species have been described in Korea; therefore, 10 species of nine genera in the order Rhizobiales and 14 species of seven genera in the order Sphingomonadales are described for unreported alphaproteobacterial species in Korea. Gram reaction, colony and cell morphology, biochemical properties, and isolation sources are also provided in the species description section.

Purification and Biological Characteristics of Alkaline Protease from Aspergillus sp. CC-29 (Aspergillus sp. CC-29 생성하는 Alkaline Protease의 정제 및 특성)

  • Choi, Cheong;Kim, Doo-Ki;Cho, Young-Je;Sung, Tae-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.5
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    • pp.434-442
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    • 1990
  • Aspergillus sp. CC-29 ws selected for its strong protease activity among various stains of molds found in soil. It was found that the production of alkaline protease reached to maximum when the wheat bran medium containing glucose as carbon source had been cultured for 4 days. Alkaline proteased was purified 36.10 fold from Aspergillus sp. CC-29 The purification procedures included ammonium sulfate fractunation gel filteration on Sepha-dex G-75 G-150 and DEAE-cellulose ion-exchange chromatography, The yield of the purified enzyme was 22.40% The purified enzyme was confirmed as a single band by the polyacryla-mide. When the purified enzyme was applied to SDS-PAGE the molecular weight was estima-ted 24000. The optimum pH for the enzyme activity was 9.0 and the optimum temperature was 4$0^{\circ}C$ The reaction of this enzyme followed typical Michaelis-Menten kinetics with the Km value of 2.10$\times$10-4M with the Vmax of 29.41 $\mu$g/min. The enzyme was reactively stable in alkalic condition and unstable by heat treatment. The activity of alkaline protease was increased by the addition of Ca2+ whereas it was inhibited by Hg2+ Zn2+ at concentration of 1$\times$10-3M.

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Different Catabolism Pathways Triggered by Various Methylxanthines in Caffeine-Tolerant Bacterium Pseudomonas putida CT25 Isolated from Tea Garden Soil

  • Ma, Yi-Xiao;Wu, Xiao-Han;Wu, Hui-Shi;Dong, Zhan-Bo;Ye, Jian-Hui;Zheng, Xin-Qiang;Liang, Yue-Rong;Lu, Jian-Liang
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1147-1155
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    • 2018
  • The degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that the degradation efficiency of various MXs varied with the number and position of the methyl groups on the molecule (i.e., xanthine > 7-methylxanthine ${\approx}$ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MX catabolism pathways coexisted in strain CT25, and a different pathway would be triggered by various MXs. Demethylation dominated in the degradation of N-7-methylated MXs (such as 7-methylxanthine, theobromine, and caffeine), where C-8 oxidation was the major pathway in the catabolism of 1-methylxanthine, whereas demethylation and C-8 oxidation are likely both involved in the degradation of theophylline. Enzymes responsible for MX degradation were located inside the cell. Both cell culture and cell-free enzyme assays revealed that N-1 demethylation might be a rate-limiting step for the catabolism of the MXs. Surprisingly, accumulation of uric acid was observed in a cell-free reaction system, which might be attributed to the lack of activity of uricase, a cytochrome c-coupled membrane integral enzyme.

Physiological properties and transformation of alkaline-tolerant bacteria (알카리내성 세균의 생리적 특성 및 형질전환)

  • 유주현;정용준;정건섭;오두환
    • Microbiology and Biotechnology Letters
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    • v.14 no.3
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    • pp.239-244
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    • 1986
  • To develop the potential use as new host strain for gene cloning, alkaline-tolerant isolates from soil were examined for amylase activity, protease activity, antimicrobial activity and transformability by using plasmid pUB 110. Of these strains, one was selected and identified as Bacillus sp. YA-14. in the enzymatic properties of Bacillus sp. YA-14 the optimal conditions for the reaction of amylase and protease were at pH 0.8 and pH 7.5 respectively. The antimicrobial activity of Bacillus sp. YA-14 was also found. For the transformation, Bacillus sp. YA-14 was cultured to late logarithmic growth phase ai 37$^{\circ}C$ in modified SPI medium (pH 8.0) containing 0.4% MgSO$_4$. The presence of pUB 110 plasmid DNA in transformants was confirmed by electrophoresis and stably maintained in the new host.

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A Survey on Green Infrastructure Design Element in Urban Hub Green - Focused on ASLA's Case Studies- (도시 거점녹지유형의 그린인프라 계획요소 고찰 -ASLA 사례분석을 중심으로-)

  • Kwon, Jin Wook;Kim, Gunwoo
    • Journal of Environmental Science International
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    • v.28 no.12
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    • pp.1147-1156
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    • 2019
  • The purpose of this study was to analyze examples of green infrastructure presented by the American Society of Landscape Architects as a part of basic research to assess hub green spaces in cities. With the specific goal of green infrasturcture in mind, the study samples were classified according to their purpose: 'humanities', 'hydrology', 'ecology', and 'environment'. Based on this we assessed the elements of planning for the target sites and obtained the following results. With regard to the aspect of humanities, planning urban hub green spaces was related to the satisfaction in leisure activities and the 'quality of life' that people expect to enjoy at parks or other green areas in general. Rather than focusing on direct and visible benefits, which might come from green infrastructure's technological elements, people hoped that parks and green areas have macroscopic values. For hydrological characteristics, the 'ecologically manages stormwater' was applied the most in planning hub green spaces in cities, and it mainly took the form of technological elements or factors. Third, the planning elements pertaining to ecological characteristics were identified as a combination of strategies and technological elements that 'reintroduces native plants' and 'habitat for wildlife'. As for the plans to instill eco-friendly aspects, the study found that the research on air, climate, weather, heat reaction, soil, energy efficiency, and use and application of resources is important. However, it was difficult to measure the potential quantitative benefits of 'reusing or recycling materials', 'reducing urban heat', and 'cooling air temperature'. The result of this study is meaningful in that it can be used for the assessment of urban hub green spaces in the future.

Properties of an Extracellular 5-Fluorocytosine Deaminase (세포외 5-Fluorocytosine Deaminase의 특성)

  • Yeeh, Yeehn;Jun, Hong-Ki;Yoon, Yong-Kyun
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.150-155
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    • 1992
  • - Some properties of an extracellular cytosine deaminase excreted from an isolate from soil samples were examined after 20~80%' ammonium sulfate fractionation. The enzyme catalyzed the conversion of cytosine and 5-fluorocytosine into uracil and 5-fluorouracil by substrate specificity, respectively. The optimum temperature and storage time on the stability of the enzvme preparation were below $50^{\circ}C$ keeping above 90% of the residual activity and near 4 days keeping above 80% of the residual one in Tris-HCI buffer. The maximum activity was also obtained at 8.0 in pH and 37'C in temperature. The pHs and temperatures for enzyme activity ranged from 8.0~8.5 and from 37~$45^{\circ}C$. respectively. The presence of $Ag^-,Hg^{2-}, Zn^{2-}, Cu^{2-}, Sn^{2-}, \; or\; Pb^{2-}$ in the reaction mixture resulted in the marked inhibition in enzyme activity, but 1 mM of $K^+, Fe^[3+}, Mg^{2+}, \; or \; Na^+$. slightly increased the activity. The enzyme preparation was heavily affected by most of inhibitors tested such as 1 mM of EIITA, NaCN and pentachlorophenol, and completely inactivated by p-CMB and TCA of 1 mM, or 10 mM.

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Hydrogeological Characteristics of the Pyeongyang Area in the 1930s (1930년대 평양지역의 수리지질 특성)

  • Oh, Yun-Yeong;Hamm, Se-Yeong;Lee, Chung-Mo;Liang, Wei Ming;Kim, Gyoo-Beom;Kim, MoonSu
    • The Journal of Engineering Geology
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    • v.24 no.4
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    • pp.583-595
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    • 2014
  • The chemical properties and composition (pH, CaO, Fe, $HCO{_3}^-$, and $SO{_4}^{2-}$) of groundwater (GW) and surface water (SW) from the northern (non-carbonate bedrock) and southern (carbonate bedrock) sections of the Daedong River, Pyeongyang were analyzed and compared period of the 1930s. In the southern section, the GW and SW has a higher pH and $SO{_4}^{2-}$ concentration, but lower $HCO{_3}^-$ and Fe levels than in the north. This finding reflects a reaction that formed acid by replacing metal ions in inorganic salts by hydrogen, which resulted from the oxidation of organic material in a clay layer.

Solumycin : A water-soluble antifungal antibiotic from Streptomyces sp. LAM-593 (Streptomyces sp. LAM-593이 생산하는 수용성 항진균성 항생물질)

  • Yi, Dong-Heui;Park, Seung-Lim;Kwon, Tae-Jong;Chung, Ho-Kwon
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.180-186
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    • 1991
  • A water soluble antifungal antibiotic, Solumycin, was separated from the culture broth of Streptomyces sp. LAM-593, isolated from soil, by butanol extraction, alumina-, 1st and 2nd Sephadex LH-20 column chromatography. The substance was pale yellow crystal which gave a single spot at Rf value 0.24 with ethanol-ammonia water-water (8:1:1), 0.46 with butanol-ethanol-water (5:1:4), 0.84 with 50% methanol on silica gel TLC. It was dissolved well in water, methanol and acidic aq. butanol but not in ethanol, acetone, ethyl acetate, chloroform. acetic acid etc., and gave positive Fehling and Molish reaction. The UV spectrum in methanol showed absorption at 342, 361, 380, and 404 nm. The antibiotic was active against fungi such as Candide, Cryptococcus, Saccharomyces, Trichophyton and Trichosporon, but not to bacteria such as Bacillus, Escherichia and Staphylococcus.

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Evaluation of Mungbean Genotypes Based on Yield Stability and Reaction to Mungbean Yellow Mosaic Virus Disease

  • Alam, A.K.M. Mahbubul;Somta, Prakit;Jompuk, Choosak;Chatwachirawong, Prasert;Srinives, Peerasak
    • The Plant Pathology Journal
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    • v.30 no.3
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    • pp.261-268
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    • 2014
  • This work was conducted to identify mungbean genotypes showing yield stability and resistance to mungbean yellow mosaic virus (MYMV) disease. Sixteen genotypes were evaluated in a randomized complete block design with two replications for two years (2011 and 2012) at three locations (Gazipur, Ishurdi and Madaripur) of the Bangladesh Agricultural Research Institute. An analysis of variance exhibited significant effects of genotype (G), environment (E), and genotype ${\times}$ environment ($G{\times}E$) on grain yield. Among eight agronomic characters, the principal component 1 (PC1) was always higher than the PC2. Considering $G{\times}E$ interaction, BM6 was the best genotype at all three locations in both years. Based on grain yield and stability performance, BM6 ranked first while the worst performing genotypes were BM1 and G10. Based on discrimination and representation, Gazipur was identified as an ideal environment for these mungbeans. Relationship between soil-plant analysis developments (SPAD) value was positive with yield but negative with MYMV severity. BM6, G1 and G2 were considered as promising sources of resistance for low disease score and stable response across the environments. The environment proved to have an influence on MYMV infection under natural infestation. A positive correlation was observed between disease score and the temperature under natural growing condition.